Neurotrophin-3 regulates ribbon synapse density in the cochlea and induces synapse regeneration after acoustic trauma

• Autores: Wan, Guoqiang; Gomez Casati, Maria Eugenia; Gigliello, Angelica R.; Liberman, M. Charles; Corfas, Gabriel
• Año de publicación: 2014
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: HAIR CELLS; TRAUMA; RIBBON SYNAPSE
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Neurotrophin-3 (Ntf3) and brain derived neurotrophic factor (Bdnf) are critical for sensory neuron survival and the establishment of neuronal projections to sensory epithelia in the embryonic inner ear, but their postnatal functions remain poorly understood. Using cell-specific inducible gene recombination in mice we found that, in the postnatal inner ear, Bbnf and Ntf3 are required for the formation and maintenance of hair cell ribbon synapses in the vestibular and cochlear epithelia, respectively. We also show that supporting cells in these epithelia are the key endogenous source of the neurotrophins. Using a new hair cell CreERT line with mosaic expression, we also found that Ntf3´s effect on cochlear synaptogenesis is highly localized. Moreover, supporting cell-derived Ntf3, but not Bbnf, promoted recovery of cochlear function and ribbon synapse regeneration after acoustic trauma. These results indicate that glial-derived neurotrophins play critical roles in inner ear synapse density and synaptic regeneration after injury.
Fil: Wan, Guoqiang. Boston Children; Estados Unidos. Harvard Medical School. Department of Neurology; Estados Unidos. University of Michigan. Department of Otolaryngology - Head and Neck Surgery. Kresge Hearing Research Institute; Estados Unidos
Fil: Gomez Casati, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina. Boston Children; Estados Unidos. Harvard Medical School. Department of Neurology; Estados Unidos
Fil: Gigliello, Angelica R.. Boston Children; Estados Unidos
Fil: Liberman, M. Charles. Harvard Medical School. Department of Otology and Laryngology; Estados Unidos. Massachusetts Eye and Ear Infirmary. Eaton Peabody Laboratories; Estados Unidos
Fil: Corfas, Gabriel. Boston Children; Estados Unidos. Harvard Medical School. Department of Neurology; Estados Unidos. University of Michigan. Department of Otolaryngology - Head and Neck Surgery. Kresge Hearing Research Institute; Estados Unidos