Use and comparison of serologic assays to detect anti-Neospora caninum antibodies in farmed red deer (Cervus elaphus)

Autores
Novoa, María Belén; Soler, Juan Pablo; Cirone, Karina Mariela; Hecker, Yanina; Valentini, Beatriz Susana; Primo, María Evangelina; Moore, Prando Dadin
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Neospora caninum is a protozoan parasite that cause abortion in different ruminant species, including red deer ( Cervus elaphus). There are no validated assays to be performed with sera from red deer. At the present work, we evaluated the agreement among indirect fluorescent antibody test (IFAT), competitive inhibition ELISA based on a recombinant protein (ciELISA tSAG1) and immunoblot (IB) to detect anti- N. caninum antibodies in a red deer herd that presented reproductive losses due to N. caninum. In addition, we analyzed the relationship between the serologic results and 15 hinds were analyzed by IFAT, ciELISA tSAG1 and IB to detect anti- N. caninum antibodies. In the three assays, the cut-off established for cattle was used. Besides, sera were analyzed by IFAT to detect anti- Toxoplasma gondii antibodies. The hinds were monitored by ultrasound scanning during the gestational period to detect abortions. Gwet’s agreement coefficient (AC1) and the percentage of agreement were used to estimate the agreement between pairs of assays. Chi-square test and odds ratio (OR) were used for the statistical association between abortion and seropositivity to N. caninum or to T. gondii. The N. caninum seropositivity rate was 53.9% (62/115), 57.4% (66/115) and 55.7% (64/115) for IFAT, ciELISA tSAG1 and IB, respectively. The AC1 and percentage of agreement were 0.760% and 87.8% for the pair ciELISA tSAG1 /IFAT, 0.793% and 89.6% for the pair IFAT/IB, and 0.966% and 98.3% for the pair IB/ciELISA tSAG1. The T. gondii seropositivity rate was 53.0% (61/115). Seropositive hinds to N. caninum were more likely to abort than seronegative hinds by the 3 assays. The OR for the association between N. caninum seropositivity and abortion was 72.70, 22.96 and 83.24 when ciELISA tSAG1, IFAT or IB assays were used, respectively. between T. gondii seropositivity and abortion. The three serologic assays were useful to detect N. caninum infected hinds. The validation of the assays for use in red deer would be an improvement for diagnosis of neosporosis in this species.
EEA Rafaela
Fil: Novoa, María Belen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Novoa, María Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Instituto de Investigación de la Cadena Láctea (IdICaL) (INTA-CONICET); Argentina
Fil: Soler, Juan Pablo. Servicio Veterinario CERVUS; Argentina.
Fil: Cirone, Karina Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Cirone, Karina Mariela. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Hecker, Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valentini, Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Valentini, Beatriz. Instituto de Investigación de la Cadena Láctea (IdICaL) (INTA-CONICET); Argentina
Fil: Primo, María Evangelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Primo, María Evangelina. Instituto de Investigación de la Cadena Láctea (IdICaL) (INTA-CONICET); Argentina
Fil: Moore, D.P. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moore, D.P. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fuente
Veterinary Parasitology 313 : 109839 (January 2023)
Materia
Neospora caninum
Neosporosis
Serology
Antibodies
ELISA
Serología
Anticuerpos
Cervus elaphus
Red Deer
Ciervo Colorado
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/13886
Acceder
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oai_identifier_str oai:localhost:20.500.12123/13886
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Use and comparison of serologic assays to detect anti-Neospora caninum antibodies in farmed red deer (Cervus elaphus)Novoa, María BelénSoler, Juan PabloCirone, Karina MarielaHecker, YaninaValentini, Beatriz SusanaPrimo, María EvangelinaMoore, Prando DadinNeospora caninumNeosporosisSerologyAntibodiesELISASerologíaAnticuerposCervus elaphusRed DeerCiervo ColoradoNeospora caninum is a protozoan parasite that cause abortion in different ruminant species, including red deer ( Cervus elaphus). There are no validated assays to be performed with sera from red deer. At the present work, we evaluated the agreement among indirect fluorescent antibody test (IFAT), competitive inhibition ELISA based on a recombinant protein (ciELISA tSAG1) and immunoblot (IB) to detect anti- N. caninum antibodies in a red deer herd that presented reproductive losses due to N. caninum. In addition, we analyzed the relationship between the serologic results and 15 hinds were analyzed by IFAT, ciELISA tSAG1 and IB to detect anti- N. caninum antibodies. In the three assays, the cut-off established for cattle was used. Besides, sera were analyzed by IFAT to detect anti- Toxoplasma gondii antibodies. The hinds were monitored by ultrasound scanning during the gestational period to detect abortions. Gwet’s agreement coefficient (AC1) and the percentage of agreement were used to estimate the agreement between pairs of assays. Chi-square test and odds ratio (OR) were used for the statistical association between abortion and seropositivity to N. caninum or to T. gondii. The N. caninum seropositivity rate was 53.9% (62/115), 57.4% (66/115) and 55.7% (64/115) for IFAT, ciELISA tSAG1 and IB, respectively. The AC1 and percentage of agreement were 0.760% and 87.8% for the pair ciELISA tSAG1 /IFAT, 0.793% and 89.6% for the pair IFAT/IB, and 0.966% and 98.3% for the pair IB/ciELISA tSAG1. The T. gondii seropositivity rate was 53.0% (61/115). Seropositive hinds to N. caninum were more likely to abort than seronegative hinds by the 3 assays. The OR for the association between N. caninum seropositivity and abortion was 72.70, 22.96 and 83.24 when ciELISA tSAG1, IFAT or IB assays were used, respectively. between T. gondii seropositivity and abortion. The three serologic assays were useful to detect N. caninum infected hinds. The validation of the assays for use in red deer would be an improvement for diagnosis of neosporosis in this species.EEA RafaelaFil: Novoa, María Belen. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Novoa, María Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Instituto de Investigación de la Cadena Láctea (IdICaL) (INTA-CONICET); ArgentinaFil: Soler, Juan Pablo. Servicio Veterinario CERVUS; Argentina.Fil: Cirone, Karina Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Cirone, Karina Mariela. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Hecker, Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Valentini, Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Valentini, Beatriz. Instituto de Investigación de la Cadena Láctea (IdICaL) (INTA-CONICET); ArgentinaFil: Primo, María Evangelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Primo, María Evangelina. Instituto de Investigación de la Cadena Láctea (IdICaL) (INTA-CONICET); ArgentinaFil: Moore, D.P. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Moore, D.P. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaElsevier2023-01-11T15:47:56Z2023-01-11T15:47:56Z2023-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/13886https://www.sciencedirect.com/science/article/pii/S0304401722001935M.B. Novoa, J.P. Soler, K.M. Cirone, Y.P. Hecker, B.S. Valentini, M.E. Primo, D.P. Moore, Use and comparison of serologic assays to detect anti-Neospora caninum antibodies in farmed red deer (Cervus elaphus), Veterinary Parasitology, Volume 313, 2023, 109839, ISSN 0304-4017, https://doi.org/10.1016/j.vetpar.2022.109839.1873-2550 (online)0304-4017 (print)https://doi.org/10.1016/j.vetpar.2022.109839Veterinary Parasitology 313 : 109839 (January 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-RIST-E5-I111-001/2019-RIST-E5-I111-001/AR./Laboratorios de Diagnóstico Veterinarioinfo:eu-repo/semantics/restrictedAccess2025-09-04T09:49:42Zoai:localhost:20.500.12123/13886instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:42.564INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Use and comparison of serologic assays to detect anti-Neospora caninum antibodies in farmed red deer (Cervus elaphus)
title Use and comparison of serologic assays to detect anti-Neospora caninum antibodies in farmed red deer (Cervus elaphus)
spellingShingle Use and comparison of serologic assays to detect anti-Neospora caninum antibodies in farmed red deer (Cervus elaphus)
Novoa, María Belén
Neospora caninum
Neosporosis
Serology
Antibodies
ELISA
Serología
Anticuerpos
Cervus elaphus
Red Deer
Ciervo Colorado
title_short Use and comparison of serologic assays to detect anti-Neospora caninum antibodies in farmed red deer (Cervus elaphus)
title_full Use and comparison of serologic assays to detect anti-Neospora caninum antibodies in farmed red deer (Cervus elaphus)
title_fullStr Use and comparison of serologic assays to detect anti-Neospora caninum antibodies in farmed red deer (Cervus elaphus)
title_full_unstemmed Use and comparison of serologic assays to detect anti-Neospora caninum antibodies in farmed red deer (Cervus elaphus)
title_sort Use and comparison of serologic assays to detect anti-Neospora caninum antibodies in farmed red deer (Cervus elaphus)
dc.creator.none.fl_str_mv Novoa, María Belén
Soler, Juan Pablo
Cirone, Karina Mariela
Hecker, Yanina
Valentini, Beatriz Susana
Primo, María Evangelina
Moore, Prando Dadin
author Novoa, María Belén
author_facet Novoa, María Belén
Soler, Juan Pablo
Cirone, Karina Mariela
Hecker, Yanina
Valentini, Beatriz Susana
Primo, María Evangelina
Moore, Prando Dadin
author_role author
author2 Soler, Juan Pablo
Cirone, Karina Mariela
Hecker, Yanina
Valentini, Beatriz Susana
Primo, María Evangelina
Moore, Prando Dadin
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Neospora caninum
Neosporosis
Serology
Antibodies
ELISA
Serología
Anticuerpos
Cervus elaphus
Red Deer
Ciervo Colorado
topic Neospora caninum
Neosporosis
Serology
Antibodies
ELISA
Serología
Anticuerpos
Cervus elaphus
Red Deer
Ciervo Colorado
dc.description.none.fl_txt_mv Neospora caninum is a protozoan parasite that cause abortion in different ruminant species, including red deer ( Cervus elaphus). There are no validated assays to be performed with sera from red deer. At the present work, we evaluated the agreement among indirect fluorescent antibody test (IFAT), competitive inhibition ELISA based on a recombinant protein (ciELISA tSAG1) and immunoblot (IB) to detect anti- N. caninum antibodies in a red deer herd that presented reproductive losses due to N. caninum. In addition, we analyzed the relationship between the serologic results and 15 hinds were analyzed by IFAT, ciELISA tSAG1 and IB to detect anti- N. caninum antibodies. In the three assays, the cut-off established for cattle was used. Besides, sera were analyzed by IFAT to detect anti- Toxoplasma gondii antibodies. The hinds were monitored by ultrasound scanning during the gestational period to detect abortions. Gwet’s agreement coefficient (AC1) and the percentage of agreement were used to estimate the agreement between pairs of assays. Chi-square test and odds ratio (OR) were used for the statistical association between abortion and seropositivity to N. caninum or to T. gondii. The N. caninum seropositivity rate was 53.9% (62/115), 57.4% (66/115) and 55.7% (64/115) for IFAT, ciELISA tSAG1 and IB, respectively. The AC1 and percentage of agreement were 0.760% and 87.8% for the pair ciELISA tSAG1 /IFAT, 0.793% and 89.6% for the pair IFAT/IB, and 0.966% and 98.3% for the pair IB/ciELISA tSAG1. The T. gondii seropositivity rate was 53.0% (61/115). Seropositive hinds to N. caninum were more likely to abort than seronegative hinds by the 3 assays. The OR for the association between N. caninum seropositivity and abortion was 72.70, 22.96 and 83.24 when ciELISA tSAG1, IFAT or IB assays were used, respectively. between T. gondii seropositivity and abortion. The three serologic assays were useful to detect N. caninum infected hinds. The validation of the assays for use in red deer would be an improvement for diagnosis of neosporosis in this species.
EEA Rafaela
Fil: Novoa, María Belen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Novoa, María Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Instituto de Investigación de la Cadena Láctea (IdICaL) (INTA-CONICET); Argentina
Fil: Soler, Juan Pablo. Servicio Veterinario CERVUS; Argentina.
Fil: Cirone, Karina Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Cirone, Karina Mariela. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Hecker, Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valentini, Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Valentini, Beatriz. Instituto de Investigación de la Cadena Láctea (IdICaL) (INTA-CONICET); Argentina
Fil: Primo, María Evangelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Primo, María Evangelina. Instituto de Investigación de la Cadena Láctea (IdICaL) (INTA-CONICET); Argentina
Fil: Moore, D.P. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moore, D.P. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
description Neospora caninum is a protozoan parasite that cause abortion in different ruminant species, including red deer ( Cervus elaphus). There are no validated assays to be performed with sera from red deer. At the present work, we evaluated the agreement among indirect fluorescent antibody test (IFAT), competitive inhibition ELISA based on a recombinant protein (ciELISA tSAG1) and immunoblot (IB) to detect anti- N. caninum antibodies in a red deer herd that presented reproductive losses due to N. caninum. In addition, we analyzed the relationship between the serologic results and 15 hinds were analyzed by IFAT, ciELISA tSAG1 and IB to detect anti- N. caninum antibodies. In the three assays, the cut-off established for cattle was used. Besides, sera were analyzed by IFAT to detect anti- Toxoplasma gondii antibodies. The hinds were monitored by ultrasound scanning during the gestational period to detect abortions. Gwet’s agreement coefficient (AC1) and the percentage of agreement were used to estimate the agreement between pairs of assays. Chi-square test and odds ratio (OR) were used for the statistical association between abortion and seropositivity to N. caninum or to T. gondii. The N. caninum seropositivity rate was 53.9% (62/115), 57.4% (66/115) and 55.7% (64/115) for IFAT, ciELISA tSAG1 and IB, respectively. The AC1 and percentage of agreement were 0.760% and 87.8% for the pair ciELISA tSAG1 /IFAT, 0.793% and 89.6% for the pair IFAT/IB, and 0.966% and 98.3% for the pair IB/ciELISA tSAG1. The T. gondii seropositivity rate was 53.0% (61/115). Seropositive hinds to N. caninum were more likely to abort than seronegative hinds by the 3 assays. The OR for the association between N. caninum seropositivity and abortion was 72.70, 22.96 and 83.24 when ciELISA tSAG1, IFAT or IB assays were used, respectively. between T. gondii seropositivity and abortion. The three serologic assays were useful to detect N. caninum infected hinds. The validation of the assays for use in red deer would be an improvement for diagnosis of neosporosis in this species.
publishDate 2023
dc.date.none.fl_str_mv 2023-01-11T15:47:56Z
2023-01-11T15:47:56Z
2023-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/13886
https://www.sciencedirect.com/science/article/pii/S0304401722001935
M.B. Novoa, J.P. Soler, K.M. Cirone, Y.P. Hecker, B.S. Valentini, M.E. Primo, D.P. Moore, Use and comparison of serologic assays to detect anti-Neospora caninum antibodies in farmed red deer (Cervus elaphus), Veterinary Parasitology, Volume 313, 2023, 109839, ISSN 0304-4017, https://doi.org/10.1016/j.vetpar.2022.109839.
1873-2550 (online)
0304-4017 (print)
https://doi.org/10.1016/j.vetpar.2022.109839
url http://hdl.handle.net/20.500.12123/13886
https://www.sciencedirect.com/science/article/pii/S0304401722001935
https://doi.org/10.1016/j.vetpar.2022.109839
identifier_str_mv M.B. Novoa, J.P. Soler, K.M. Cirone, Y.P. Hecker, B.S. Valentini, M.E. Primo, D.P. Moore, Use and comparison of serologic assays to detect anti-Neospora caninum antibodies in farmed red deer (Cervus elaphus), Veterinary Parasitology, Volume 313, 2023, 109839, ISSN 0304-4017, https://doi.org/10.1016/j.vetpar.2022.109839.
1873-2550 (online)
0304-4017 (print)
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-RIST-E5-I111-001/2019-RIST-E5-I111-001/AR./Laboratorios de Diagnóstico Veterinario
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary Parasitology 313 : 109839 (January 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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