Lipid changes in rat spermatozoa during isolation and functional activation in vitro

Autores
Oresti, Gerardo Martin; Peñalva, Daniel Alejandro; Luquez, Jessica Mariela; Antollini, Silvia Susana; Aveldaño, Marta Isabel
Año de publicación
2012
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Hydrolysis of glycerophospholipids (GPL) and sphingomyelin (SM) are calcium-dependent reactions that are significantly stimulated in rat epididymal spermatozoa during incubations leading to capacitation (Cap) and particularly after acrosomal reaction (AR). In comparison with gametes whose contact during isolation with tissue divalent cations was avoided (C1), or kept to a minimum (C2), those used as controls in these incubations (C3) already had their glycerophospholipids (GPL) and sphingomyelin (SM) partially hydrolyzed. Using C1 as controls, the C3 gametes had less GPL per cell and more free fatty acids. The latter were released with cholesterol from cells to media during albumindependent capacitation. Significant decreases in membrane GPL occurred gradually in the order C1 > C2 > C3 > Cap > AR. Diminution in the major choline GPL specifically targeted phosphatidylcholine in the presence of a virtually unchanged amount of plasmenylcholine, the acrosome-reacted cells thus becoming relatively rich in plasmalogens. The unique SM with very long chain PUFA located on sperm heads was intact in C1 cells and maximally hydrolyzed into ceramides after completion of AR. Using fluorescent probes, liposomes prepared from the 5 conditions showed that C1 gametes had the minimun degree of order of the lipid bilayer, this increasing significantly as Cap andAR proceeded
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Peñalva, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Antollini, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XLVIII Reunión Nacional de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Mendoza
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Materia
SPHINGOLIPIDS
CERAMIDE
SPERMATOZOA
PLASMALOGENS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/235774
Acceder
id CONICETDig_f398b5dff64682c6e67b6552817ecaba
oai_identifier_str oai:ri.conicet.gov.ar:11336/235774
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lipid changes in rat spermatozoa during isolation and functional activation in vitroOresti, Gerardo MartinPeñalva, Daniel AlejandroLuquez, Jessica MarielaAntollini, Silvia SusanaAveldaño, Marta IsabelSPHINGOLIPIDSCERAMIDESPERMATOZOAPLASMALOGENShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Hydrolysis of glycerophospholipids (GPL) and sphingomyelin (SM) are calcium-dependent reactions that are significantly stimulated in rat epididymal spermatozoa during incubations leading to capacitation (Cap) and particularly after acrosomal reaction (AR). In comparison with gametes whose contact during isolation with tissue divalent cations was avoided (C1), or kept to a minimum (C2), those used as controls in these incubations (C3) already had their glycerophospholipids (GPL) and sphingomyelin (SM) partially hydrolyzed. Using C1 as controls, the C3 gametes had less GPL per cell and more free fatty acids. The latter were released with cholesterol from cells to media during albumindependent capacitation. Significant decreases in membrane GPL occurred gradually in the order C1 > C2 > C3 > Cap > AR. Diminution in the major choline GPL specifically targeted phosphatidylcholine in the presence of a virtually unchanged amount of plasmenylcholine, the acrosome-reacted cells thus becoming relatively rich in plasmalogens. The unique SM with very long chain PUFA located on sperm heads was intact in C1 cells and maximally hydrolyzed into ceramides after completion of AR. Using fluorescent probes, liposomes prepared from the 5 conditions showed that C1 gametes had the minimun degree of order of the lipid bilayer, this increasing significantly as Cap andAR proceededFil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Peñalva, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Antollini, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaXLVIII Reunión Nacional de la Sociedad Argentina de Investigación Bioquímica y Biología MolecularMendozaArgentinaSociedad Argentina de Investigación Bioquímica y Biología MolecularReunión Anual th Argentine Society for Biochemistry and Molecular Biology Sociedad Argentina de Investigación en Bioquímica y Biología Molecular2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/235774Lipid changes in rat spermatozoa during isolation and functional activation in vitro; XLVIII Reunión Nacional de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; Mendoza; Argentina; 2012; 52-520327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://newsite.saib.org.ar/publicaciones/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:01:11Zoai:ri.conicet.gov.ar:11336/235774instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:01:11.482CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lipid changes in rat spermatozoa during isolation and functional activation in vitro
title Lipid changes in rat spermatozoa during isolation and functional activation in vitro
spellingShingle Lipid changes in rat spermatozoa during isolation and functional activation in vitro
Oresti, Gerardo Martin
SPHINGOLIPIDS
CERAMIDE
SPERMATOZOA
PLASMALOGENS
title_short Lipid changes in rat spermatozoa during isolation and functional activation in vitro
title_full Lipid changes in rat spermatozoa during isolation and functional activation in vitro
title_fullStr Lipid changes in rat spermatozoa during isolation and functional activation in vitro
title_full_unstemmed Lipid changes in rat spermatozoa during isolation and functional activation in vitro
title_sort Lipid changes in rat spermatozoa during isolation and functional activation in vitro
dc.creator.none.fl_str_mv Oresti, Gerardo Martin
Peñalva, Daniel Alejandro
Luquez, Jessica Mariela
Antollini, Silvia Susana
Aveldaño, Marta Isabel
author Oresti, Gerardo Martin
author_facet Oresti, Gerardo Martin
Peñalva, Daniel Alejandro
Luquez, Jessica Mariela
Antollini, Silvia Susana
Aveldaño, Marta Isabel
author_role author
author2 Peñalva, Daniel Alejandro
Luquez, Jessica Mariela
Antollini, Silvia Susana
Aveldaño, Marta Isabel
author2_role author
author
author
author
dc.subject.none.fl_str_mv SPHINGOLIPIDS
CERAMIDE
SPERMATOZOA
PLASMALOGENS
topic SPHINGOLIPIDS
CERAMIDE
SPERMATOZOA
PLASMALOGENS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Hydrolysis of glycerophospholipids (GPL) and sphingomyelin (SM) are calcium-dependent reactions that are significantly stimulated in rat epididymal spermatozoa during incubations leading to capacitation (Cap) and particularly after acrosomal reaction (AR). In comparison with gametes whose contact during isolation with tissue divalent cations was avoided (C1), or kept to a minimum (C2), those used as controls in these incubations (C3) already had their glycerophospholipids (GPL) and sphingomyelin (SM) partially hydrolyzed. Using C1 as controls, the C3 gametes had less GPL per cell and more free fatty acids. The latter were released with cholesterol from cells to media during albumindependent capacitation. Significant decreases in membrane GPL occurred gradually in the order C1 > C2 > C3 > Cap > AR. Diminution in the major choline GPL specifically targeted phosphatidylcholine in the presence of a virtually unchanged amount of plasmenylcholine, the acrosome-reacted cells thus becoming relatively rich in plasmalogens. The unique SM with very long chain PUFA located on sperm heads was intact in C1 cells and maximally hydrolyzed into ceramides after completion of AR. Using fluorescent probes, liposomes prepared from the 5 conditions showed that C1 gametes had the minimun degree of order of the lipid bilayer, this increasing significantly as Cap andAR proceeded
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Peñalva, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Antollini, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XLVIII Reunión Nacional de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Mendoza
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
description Hydrolysis of glycerophospholipids (GPL) and sphingomyelin (SM) are calcium-dependent reactions that are significantly stimulated in rat epididymal spermatozoa during incubations leading to capacitation (Cap) and particularly after acrosomal reaction (AR). In comparison with gametes whose contact during isolation with tissue divalent cations was avoided (C1), or kept to a minimum (C2), those used as controls in these incubations (C3) already had their glycerophospholipids (GPL) and sphingomyelin (SM) partially hydrolyzed. Using C1 as controls, the C3 gametes had less GPL per cell and more free fatty acids. The latter were released with cholesterol from cells to media during albumindependent capacitation. Significant decreases in membrane GPL occurred gradually in the order C1 > C2 > C3 > Cap > AR. Diminution in the major choline GPL specifically targeted phosphatidylcholine in the presence of a virtually unchanged amount of plasmenylcholine, the acrosome-reacted cells thus becoming relatively rich in plasmalogens. The unique SM with very long chain PUFA located on sperm heads was intact in C1 cells and maximally hydrolyzed into ceramides after completion of AR. Using fluorescent probes, liposomes prepared from the 5 conditions showed that C1 gametes had the minimun degree of order of the lipid bilayer, this increasing significantly as Cap andAR proceeded
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/235774
Lipid changes in rat spermatozoa during isolation and functional activation in vitro; XLVIII Reunión Nacional de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; Mendoza; Argentina; 2012; 52-52
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/235774
identifier_str_mv Lipid changes in rat spermatozoa during isolation and functional activation in vitro; XLVIII Reunión Nacional de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; Mendoza; Argentina; 2012; 52-52
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://newsite.saib.org.ar/publicaciones/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Reunión Anual th Argentine Society for Biochemistry and Molecular Biology Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
publisher.none.fl_str_mv Reunión Anual th Argentine Society for Biochemistry and Molecular Biology Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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