Intestinal/uterine antispasmodics, sedative effects of Fuchsia magellanica Lam. leaves’ and flowers’ extracts and their flavonolic components

Autores
Bernal Ochoa, Ana María; Colareda, German Andres; Matera, Soledad Inés; Ragone, María Inés; Isolabella Capua, Santiago Alfredo; Filip, Rosana; Consolini, Alicia Elvira
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Fuchsia magellanica Lam. ( Onagraceae ) is a native plant of the Andean-Patagonian region, and it is also naturalized in Argentina and other parts of the world. Leaves are used for treating indigestion, stomachache, as sedative, for difficult delivery and as antiemmenagogue. However, up to now the pharmacological basis of such uses were not studied. Purpose: This work evaluates the gastrointestinal, uterine and sedative effects of leaves (L) and flowers (F) tinc- tures of F. magellanica from wild patagonian (T-Fm-P) and naturalized (T-Fm-BA) plants, as well as the mechanism of action and the flavonoids profile of both plants. Methods: Phytochemical studies were evaluated by using TLC and HPLC methodologies. The ex-vivo effects of T-Fm-BA and T-Fm-P were evaluated on contractile concentration-response curves (CRC) of carbachol (CCh) and calcium (Ca 2 + ) in rat isolated intestinal and uterine tissues. In vivo tests for intestinal transit, elevated cross plus-maze and open-field tests were performed in mice. Results: Wild and cultivated leaves and flowers ethanolic extracts induced antispasmodic effect, as a non- competitive inhibitor of the CCh-CRC in intestine, with IC 50 of 272.8 ± 64.3 μg/ml for T-L-Fm-P and 257.4 ± 36 μg/ml for T-L-Fm-BA. A similar effect was obtained in Ca 2 + -CRC with IC 50 of 152.9 ± 29.1 μg/ml and 138.4 ± 48.6 μg/ml respectively. Mice intestinal transit was reduced at oral doses of 73.5 mg/kg of T-L-Fm-P. In uterine tissues, both T-L-Fm reduced the contractions of CCh CRC´s as non-competitive antagonists, with IC 50 near those in intestine. The T-L-Fm-BA also inhibited the serotonin CRC´s and contribution of other relaxing mechanisms was investigated. Although extracts didn?t show anxiolytic effect in the elevated cross plus-maze test, they reduced the spontaneous activity of mice in the open-field at 32.7 mg/kg T-L-Fm-P and 41.7 mg/kg T-L-Fm-BA. Conclusions: Leaves and flowers ethanolic extracts from wild and cultivated plant F. magellanica showed intesti- nal and uterine antispasmodic effects, mainly by interfering with Ca 2 + influx. In mice, the ethanolic extracts reduced the intestinal transit and showed sedative effect. The effects agree with the presence of flavonoids, such as quercetin and kaempferol. This is the first study which gives experimental support for some of the traditional uses of F. magellanica .
Fil: Bernal Ochoa, Ana María. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina
Fil: Colareda, German Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina
Fil: Matera, Soledad Inés. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina
Fil: Ragone, María Inés. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Isolabella Capua, Santiago Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Filip, Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Consolini, Alicia Elvira. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina
Materia
FUCHSIA MAGELLANICA
ANTISPASMODIC
INTESTINE
OPEN-FIELD TEST
UTERUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/150256
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Intestinal/uterine antispasmodics, sedative effects of Fuchsia magellanica Lam. leaves’ and flowers’ extracts and their flavonolic componentsBernal Ochoa, Ana MaríaColareda, German AndresMatera, Soledad InésRagone, María InésIsolabella Capua, Santiago AlfredoFilip, RosanaConsolini, Alicia ElviraFUCHSIA MAGELLANICAANTISPASMODICINTESTINEOPEN-FIELD TESTUTERUShttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3Background: Fuchsia magellanica Lam. ( Onagraceae ) is a native plant of the Andean-Patagonian region, and it is also naturalized in Argentina and other parts of the world. Leaves are used for treating indigestion, stomachache, as sedative, for difficult delivery and as antiemmenagogue. However, up to now the pharmacological basis of such uses were not studied. Purpose: This work evaluates the gastrointestinal, uterine and sedative effects of leaves (L) and flowers (F) tinc- tures of F. magellanica from wild patagonian (T-Fm-P) and naturalized (T-Fm-BA) plants, as well as the mechanism of action and the flavonoids profile of both plants. Methods: Phytochemical studies were evaluated by using TLC and HPLC methodologies. The ex-vivo effects of T-Fm-BA and T-Fm-P were evaluated on contractile concentration-response curves (CRC) of carbachol (CCh) and calcium (Ca 2 + ) in rat isolated intestinal and uterine tissues. In vivo tests for intestinal transit, elevated cross plus-maze and open-field tests were performed in mice. Results: Wild and cultivated leaves and flowers ethanolic extracts induced antispasmodic effect, as a non- competitive inhibitor of the CCh-CRC in intestine, with IC 50 of 272.8 ± 64.3 μg/ml for T-L-Fm-P and 257.4 ± 36 μg/ml for T-L-Fm-BA. A similar effect was obtained in Ca 2 + -CRC with IC 50 of 152.9 ± 29.1 μg/ml and 138.4 ± 48.6 μg/ml respectively. Mice intestinal transit was reduced at oral doses of 73.5 mg/kg of T-L-Fm-P. In uterine tissues, both T-L-Fm reduced the contractions of CCh CRC´s as non-competitive antagonists, with IC 50 near those in intestine. The T-L-Fm-BA also inhibited the serotonin CRC´s and contribution of other relaxing mechanisms was investigated. Although extracts didn?t show anxiolytic effect in the elevated cross plus-maze test, they reduced the spontaneous activity of mice in the open-field at 32.7 mg/kg T-L-Fm-P and 41.7 mg/kg T-L-Fm-BA. Conclusions: Leaves and flowers ethanolic extracts from wild and cultivated plant F. magellanica showed intesti- nal and uterine antispasmodic effects, mainly by interfering with Ca 2 + influx. In mice, the ethanolic extracts reduced the intestinal transit and showed sedative effect. The effects agree with the presence of flavonoids, such as quercetin and kaempferol. This is the first study which gives experimental support for some of the traditional uses of F. magellanica .Fil: Bernal Ochoa, Ana María. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; ArgentinaFil: Colareda, German Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; ArgentinaFil: Matera, Soledad Inés. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; ArgentinaFil: Ragone, María Inés. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Isolabella Capua, Santiago Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Filip, Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Consolini, Alicia Elvira. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; ArgentinaElsevier2021-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/150256Bernal Ochoa, Ana María; Colareda, German Andres; Matera, Soledad Inés; Ragone, María Inés; Isolabella Capua, Santiago Alfredo; et al.; Intestinal/uterine antispasmodics, sedative effects of Fuchsia magellanica Lam. leaves’ and flowers’ extracts and their flavonolic components; Elsevier; Phytomedicine Plus; 1; 4; 11-2021; 1-102667-0313CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2667031321000427info:eu-repo/semantics/altIdentifier/doi/10.1016/j.phyplu.2021.100060info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:11:13Zoai:ri.conicet.gov.ar:11336/150256instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:11:13.587CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Intestinal/uterine antispasmodics, sedative effects of Fuchsia magellanica Lam. leaves’ and flowers’ extracts and their flavonolic components
title Intestinal/uterine antispasmodics, sedative effects of Fuchsia magellanica Lam. leaves’ and flowers’ extracts and their flavonolic components
spellingShingle Intestinal/uterine antispasmodics, sedative effects of Fuchsia magellanica Lam. leaves’ and flowers’ extracts and their flavonolic components
Bernal Ochoa, Ana María
FUCHSIA MAGELLANICA
ANTISPASMODIC
INTESTINE
OPEN-FIELD TEST
UTERUS
title_short Intestinal/uterine antispasmodics, sedative effects of Fuchsia magellanica Lam. leaves’ and flowers’ extracts and their flavonolic components
title_full Intestinal/uterine antispasmodics, sedative effects of Fuchsia magellanica Lam. leaves’ and flowers’ extracts and their flavonolic components
title_fullStr Intestinal/uterine antispasmodics, sedative effects of Fuchsia magellanica Lam. leaves’ and flowers’ extracts and their flavonolic components
title_full_unstemmed Intestinal/uterine antispasmodics, sedative effects of Fuchsia magellanica Lam. leaves’ and flowers’ extracts and their flavonolic components
title_sort Intestinal/uterine antispasmodics, sedative effects of Fuchsia magellanica Lam. leaves’ and flowers’ extracts and their flavonolic components
dc.creator.none.fl_str_mv Bernal Ochoa, Ana María
Colareda, German Andres
Matera, Soledad Inés
Ragone, María Inés
Isolabella Capua, Santiago Alfredo
Filip, Rosana
Consolini, Alicia Elvira
author Bernal Ochoa, Ana María
author_facet Bernal Ochoa, Ana María
Colareda, German Andres
Matera, Soledad Inés
Ragone, María Inés
Isolabella Capua, Santiago Alfredo
Filip, Rosana
Consolini, Alicia Elvira
author_role author
author2 Colareda, German Andres
Matera, Soledad Inés
Ragone, María Inés
Isolabella Capua, Santiago Alfredo
Filip, Rosana
Consolini, Alicia Elvira
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv FUCHSIA MAGELLANICA
ANTISPASMODIC
INTESTINE
OPEN-FIELD TEST
UTERUS
topic FUCHSIA MAGELLANICA
ANTISPASMODIC
INTESTINE
OPEN-FIELD TEST
UTERUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: Fuchsia magellanica Lam. ( Onagraceae ) is a native plant of the Andean-Patagonian region, and it is also naturalized in Argentina and other parts of the world. Leaves are used for treating indigestion, stomachache, as sedative, for difficult delivery and as antiemmenagogue. However, up to now the pharmacological basis of such uses were not studied. Purpose: This work evaluates the gastrointestinal, uterine and sedative effects of leaves (L) and flowers (F) tinc- tures of F. magellanica from wild patagonian (T-Fm-P) and naturalized (T-Fm-BA) plants, as well as the mechanism of action and the flavonoids profile of both plants. Methods: Phytochemical studies were evaluated by using TLC and HPLC methodologies. The ex-vivo effects of T-Fm-BA and T-Fm-P were evaluated on contractile concentration-response curves (CRC) of carbachol (CCh) and calcium (Ca 2 + ) in rat isolated intestinal and uterine tissues. In vivo tests for intestinal transit, elevated cross plus-maze and open-field tests were performed in mice. Results: Wild and cultivated leaves and flowers ethanolic extracts induced antispasmodic effect, as a non- competitive inhibitor of the CCh-CRC in intestine, with IC 50 of 272.8 ± 64.3 μg/ml for T-L-Fm-P and 257.4 ± 36 μg/ml for T-L-Fm-BA. A similar effect was obtained in Ca 2 + -CRC with IC 50 of 152.9 ± 29.1 μg/ml and 138.4 ± 48.6 μg/ml respectively. Mice intestinal transit was reduced at oral doses of 73.5 mg/kg of T-L-Fm-P. In uterine tissues, both T-L-Fm reduced the contractions of CCh CRC´s as non-competitive antagonists, with IC 50 near those in intestine. The T-L-Fm-BA also inhibited the serotonin CRC´s and contribution of other relaxing mechanisms was investigated. Although extracts didn?t show anxiolytic effect in the elevated cross plus-maze test, they reduced the spontaneous activity of mice in the open-field at 32.7 mg/kg T-L-Fm-P and 41.7 mg/kg T-L-Fm-BA. Conclusions: Leaves and flowers ethanolic extracts from wild and cultivated plant F. magellanica showed intesti- nal and uterine antispasmodic effects, mainly by interfering with Ca 2 + influx. In mice, the ethanolic extracts reduced the intestinal transit and showed sedative effect. The effects agree with the presence of flavonoids, such as quercetin and kaempferol. This is the first study which gives experimental support for some of the traditional uses of F. magellanica .
Fil: Bernal Ochoa, Ana María. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina
Fil: Colareda, German Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina
Fil: Matera, Soledad Inés. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina
Fil: Ragone, María Inés. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Isolabella Capua, Santiago Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Filip, Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Consolini, Alicia Elvira. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina
description Background: Fuchsia magellanica Lam. ( Onagraceae ) is a native plant of the Andean-Patagonian region, and it is also naturalized in Argentina and other parts of the world. Leaves are used for treating indigestion, stomachache, as sedative, for difficult delivery and as antiemmenagogue. However, up to now the pharmacological basis of such uses were not studied. Purpose: This work evaluates the gastrointestinal, uterine and sedative effects of leaves (L) and flowers (F) tinc- tures of F. magellanica from wild patagonian (T-Fm-P) and naturalized (T-Fm-BA) plants, as well as the mechanism of action and the flavonoids profile of both plants. Methods: Phytochemical studies were evaluated by using TLC and HPLC methodologies. The ex-vivo effects of T-Fm-BA and T-Fm-P were evaluated on contractile concentration-response curves (CRC) of carbachol (CCh) and calcium (Ca 2 + ) in rat isolated intestinal and uterine tissues. In vivo tests for intestinal transit, elevated cross plus-maze and open-field tests were performed in mice. Results: Wild and cultivated leaves and flowers ethanolic extracts induced antispasmodic effect, as a non- competitive inhibitor of the CCh-CRC in intestine, with IC 50 of 272.8 ± 64.3 μg/ml for T-L-Fm-P and 257.4 ± 36 μg/ml for T-L-Fm-BA. A similar effect was obtained in Ca 2 + -CRC with IC 50 of 152.9 ± 29.1 μg/ml and 138.4 ± 48.6 μg/ml respectively. Mice intestinal transit was reduced at oral doses of 73.5 mg/kg of T-L-Fm-P. In uterine tissues, both T-L-Fm reduced the contractions of CCh CRC´s as non-competitive antagonists, with IC 50 near those in intestine. The T-L-Fm-BA also inhibited the serotonin CRC´s and contribution of other relaxing mechanisms was investigated. Although extracts didn?t show anxiolytic effect in the elevated cross plus-maze test, they reduced the spontaneous activity of mice in the open-field at 32.7 mg/kg T-L-Fm-P and 41.7 mg/kg T-L-Fm-BA. Conclusions: Leaves and flowers ethanolic extracts from wild and cultivated plant F. magellanica showed intesti- nal and uterine antispasmodic effects, mainly by interfering with Ca 2 + influx. In mice, the ethanolic extracts reduced the intestinal transit and showed sedative effect. The effects agree with the presence of flavonoids, such as quercetin and kaempferol. This is the first study which gives experimental support for some of the traditional uses of F. magellanica .
publishDate 2021
dc.date.none.fl_str_mv 2021-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/150256
Bernal Ochoa, Ana María; Colareda, German Andres; Matera, Soledad Inés; Ragone, María Inés; Isolabella Capua, Santiago Alfredo; et al.; Intestinal/uterine antispasmodics, sedative effects of Fuchsia magellanica Lam. leaves’ and flowers’ extracts and their flavonolic components; Elsevier; Phytomedicine Plus; 1; 4; 11-2021; 1-10
2667-0313
CONICET Digital
CONICET
url http://hdl.handle.net/11336/150256
identifier_str_mv Bernal Ochoa, Ana María; Colareda, German Andres; Matera, Soledad Inés; Ragone, María Inés; Isolabella Capua, Santiago Alfredo; et al.; Intestinal/uterine antispasmodics, sedative effects of Fuchsia magellanica Lam. leaves’ and flowers’ extracts and their flavonolic components; Elsevier; Phytomedicine Plus; 1; 4; 11-2021; 1-10
2667-0313
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2667031321000427
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.phyplu.2021.100060
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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