Lactic acid bacteria isolated from fish gut produce conjugated linoleic acid without the addition of exogenous substrate

Autores
Vela Gurovic, Maria Soledad; Gentili, Alejandro Raúl; Olivera, Nelda Lila; Rodriguez, Maria Susana
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The production of conjugated linoleic acid (CLA) by four strains of lactic acid bacteria isolated from fish, i.e., Leuconostoc mesenteroides H20, Leuconostoc mesenteroides H22, Leuconostoc lactis H24 and Lactobacil- lus pentosus H16, was evaluated in MRS broth and on MRS agar. The bioconversion and production of CLA by resting cells were also assessed. Linoleic acid was detected in cultures grown on agar at per- centages of up to 18.3% (w/w) of total fatty acid, and conjugated isomers were found in the fatty acid profiles of Lactobacillus pentosus H16. The percentage of CLA relative to total fatty acid increased from 5.68 ± 1.65% to 23.69 ± 0.79% when resting cells were removed from agar plates and incubated with- out the addition of exogenous linoleic acid as a substrate. When Lactobacillus pentosus H16 cells were incubated with linoleic acid, cyclization and changes in monounsaturated fatty acid percentages were observed instead of conjugation. These results show that growth on a solid support is required for CLA production. More significantly, an increase in the CLA content could be achieved by incubating resting cells without exogenous substrate.
Fil: Vela Gurovic, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Gentili, Alejandro Raúl. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Olivera, Nelda Lila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico; Argentina
Fil: Rodriguez, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Materia
Conjugated Linoleic Acid
Lactic Acic Bacteria
Fish
Linoleic Acid
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/20537
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/20537
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lactic acid bacteria isolated from fish gut produce conjugated linoleic acid without the addition of exogenous substrateVela Gurovic, Maria SoledadGentili, Alejandro RaúlOlivera, Nelda LilaRodriguez, Maria SusanaConjugated Linoleic AcidLactic Acic BacteriaFishLinoleic Acidhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2The production of conjugated linoleic acid (CLA) by four strains of lactic acid bacteria isolated from fish, i.e., Leuconostoc mesenteroides H20, Leuconostoc mesenteroides H22, Leuconostoc lactis H24 and Lactobacil- lus pentosus H16, was evaluated in MRS broth and on MRS agar. The bioconversion and production of CLA by resting cells were also assessed. Linoleic acid was detected in cultures grown on agar at per- centages of up to 18.3% (w/w) of total fatty acid, and conjugated isomers were found in the fatty acid profiles of Lactobacillus pentosus H16. The percentage of CLA relative to total fatty acid increased from 5.68 ± 1.65% to 23.69 ± 0.79% when resting cells were removed from agar plates and incubated with- out the addition of exogenous linoleic acid as a substrate. When Lactobacillus pentosus H16 cells were incubated with linoleic acid, cyclization and changes in monounsaturated fatty acid percentages were observed instead of conjugation. These results show that growth on a solid support is required for CLA production. More significantly, an increase in the CLA content could be achieved by incubating resting cells without exogenous substrate.Fil: Vela Gurovic, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Gentili, Alejandro Raúl. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Olivera, Nelda Lila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico; ArgentinaFil: Rodriguez, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaElsevier2014-04-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/20537Vela Gurovic, Maria Soledad; Gentili, Alejandro Raúl; Olivera, Nelda Lila; Rodriguez, Maria Susana; Lactic acid bacteria isolated from fish gut produce conjugated linoleic acid without the addition of exogenous substrate; Elsevier; Process Biochemistry; 49; 7; 16-4-2014; 1071-10771359-5113CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.procbio.2014.04.004info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1359511314002281info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:54Zoai:ri.conicet.gov.ar:11336/20537instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:54.483CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lactic acid bacteria isolated from fish gut produce conjugated linoleic acid without the addition of exogenous substrate
title Lactic acid bacteria isolated from fish gut produce conjugated linoleic acid without the addition of exogenous substrate
spellingShingle Lactic acid bacteria isolated from fish gut produce conjugated linoleic acid without the addition of exogenous substrate
Vela Gurovic, Maria Soledad
Conjugated Linoleic Acid
Lactic Acic Bacteria
Fish
Linoleic Acid
title_short Lactic acid bacteria isolated from fish gut produce conjugated linoleic acid without the addition of exogenous substrate
title_full Lactic acid bacteria isolated from fish gut produce conjugated linoleic acid without the addition of exogenous substrate
title_fullStr Lactic acid bacteria isolated from fish gut produce conjugated linoleic acid without the addition of exogenous substrate
title_full_unstemmed Lactic acid bacteria isolated from fish gut produce conjugated linoleic acid without the addition of exogenous substrate
title_sort Lactic acid bacteria isolated from fish gut produce conjugated linoleic acid without the addition of exogenous substrate
dc.creator.none.fl_str_mv Vela Gurovic, Maria Soledad
Gentili, Alejandro Raúl
Olivera, Nelda Lila
Rodriguez, Maria Susana
author Vela Gurovic, Maria Soledad
author_facet Vela Gurovic, Maria Soledad
Gentili, Alejandro Raúl
Olivera, Nelda Lila
Rodriguez, Maria Susana
author_role author
author2 Gentili, Alejandro Raúl
Olivera, Nelda Lila
Rodriguez, Maria Susana
author2_role author
author
author
dc.subject.none.fl_str_mv Conjugated Linoleic Acid
Lactic Acic Bacteria
Fish
Linoleic Acid
topic Conjugated Linoleic Acid
Lactic Acic Bacteria
Fish
Linoleic Acid
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The production of conjugated linoleic acid (CLA) by four strains of lactic acid bacteria isolated from fish, i.e., Leuconostoc mesenteroides H20, Leuconostoc mesenteroides H22, Leuconostoc lactis H24 and Lactobacil- lus pentosus H16, was evaluated in MRS broth and on MRS agar. The bioconversion and production of CLA by resting cells were also assessed. Linoleic acid was detected in cultures grown on agar at per- centages of up to 18.3% (w/w) of total fatty acid, and conjugated isomers were found in the fatty acid profiles of Lactobacillus pentosus H16. The percentage of CLA relative to total fatty acid increased from 5.68 ± 1.65% to 23.69 ± 0.79% when resting cells were removed from agar plates and incubated with- out the addition of exogenous linoleic acid as a substrate. When Lactobacillus pentosus H16 cells were incubated with linoleic acid, cyclization and changes in monounsaturated fatty acid percentages were observed instead of conjugation. These results show that growth on a solid support is required for CLA production. More significantly, an increase in the CLA content could be achieved by incubating resting cells without exogenous substrate.
Fil: Vela Gurovic, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Gentili, Alejandro Raúl. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Olivera, Nelda Lila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico; Argentina
Fil: Rodriguez, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
description The production of conjugated linoleic acid (CLA) by four strains of lactic acid bacteria isolated from fish, i.e., Leuconostoc mesenteroides H20, Leuconostoc mesenteroides H22, Leuconostoc lactis H24 and Lactobacil- lus pentosus H16, was evaluated in MRS broth and on MRS agar. The bioconversion and production of CLA by resting cells were also assessed. Linoleic acid was detected in cultures grown on agar at per- centages of up to 18.3% (w/w) of total fatty acid, and conjugated isomers were found in the fatty acid profiles of Lactobacillus pentosus H16. The percentage of CLA relative to total fatty acid increased from 5.68 ± 1.65% to 23.69 ± 0.79% when resting cells were removed from agar plates and incubated with- out the addition of exogenous linoleic acid as a substrate. When Lactobacillus pentosus H16 cells were incubated with linoleic acid, cyclization and changes in monounsaturated fatty acid percentages were observed instead of conjugation. These results show that growth on a solid support is required for CLA production. More significantly, an increase in the CLA content could be achieved by incubating resting cells without exogenous substrate.
publishDate 2014
dc.date.none.fl_str_mv 2014-04-16
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/20537
Vela Gurovic, Maria Soledad; Gentili, Alejandro Raúl; Olivera, Nelda Lila; Rodriguez, Maria Susana; Lactic acid bacteria isolated from fish gut produce conjugated linoleic acid without the addition of exogenous substrate; Elsevier; Process Biochemistry; 49; 7; 16-4-2014; 1071-1077
1359-5113
CONICET Digital
CONICET
url http://hdl.handle.net/11336/20537
identifier_str_mv Vela Gurovic, Maria Soledad; Gentili, Alejandro Raúl; Olivera, Nelda Lila; Rodriguez, Maria Susana; Lactic acid bacteria isolated from fish gut produce conjugated linoleic acid without the addition of exogenous substrate; Elsevier; Process Biochemistry; 49; 7; 16-4-2014; 1071-1077
1359-5113
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.procbio.2014.04.004
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1359511314002281
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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