Conjugated linoleic acid is a preferential substrate for fatty acid nitration

Autores
Bonacci, Gustavo Roberto; Baker, Paul R. S.; Salvatore, Sonia Rosana; Shores, Darla; Khoo, Nicholas K. H.; Koenitzer, Jeffrey R.; Vitturi, Dario A.; Woodcock, Steven R.; Golin-Bisello, Franca; Cole, Marsha P.; Watkins, Simon; St. Croix, Claudette; Batthyany, Carlos I.; Freeman, Bruce A.; Schopfer, Francisco J.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The oxidation and nitration of unsaturated fatty acids by oxides of nitrogen yield electrophilic derivatives that can modulate protein function via post-translational protein modifications. The biological mechanisms accounting for fatty acid nitration and the specific structural characteristics of products remain to be defined. Herein, conjugated linoleic acid (CLA) is identified as the primary endogenous substrate for fatty acid nitration in vitro and in vivo, yielding up to 105 greater extent of nitration products as compared with bis-allylic linoleic acid. Multiple enzymatic and cellular mechanisms account for CLA nitration, including reactions catalyzed by mitochondria, activated macrophages, and gastric acidification. Nitroalkene derivatives of CLA and their metabolites are detected in the plasma of healthy humans and are increased in tissues undergoing episodes of ischemia reperfusion. Dietary CLA and nitrite supplementation in rodents elevates NO2-CLA levels in plasma, urine, and tissues, which in turn induces heme oxygenase-1 (HO-1) expression in the colonic epithelium. These results affirm that metabolic and inflammatory reactions yield electrophilic products that can modulate adaptive cell signaling mechanisms.
Fil: Bonacci, Gustavo Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados Unidos
Fil: Baker, Paul R. S.. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados Unidos
Fil: Salvatore, Sonia Rosana. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados Unidos
Fil: Shores, Darla. University of Pittsburgh; Estados Unidos
Fil: Khoo, Nicholas K. H.. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados Unidos
Fil: Koenitzer, Jeffrey R.. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados Unidos
Fil: Vitturi, Dario A.. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados Unidos
Fil: Woodcock, Steven R.. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados Unidos
Fil: Golin-Bisello, Franca. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados Unidos
Fil: Cole, Marsha P.. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados Unidos
Fil: Watkins, Simon. University of Pittsburgh; Estados Unidos
Fil: St. Croix, Claudette. University of Pittsburgh; Estados Unidos
Fil: Batthyany, Carlos I.. Instituto Pasteur; Uruguay. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados Unidos
Fil: Freeman, Bruce A.. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados Unidos
Fil: Schopfer, Francisco J.. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados Unidos
Materia
Fatty Acid Nitration
Conjugated Linoleic Acid
Nitration
Nitrite
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54763
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/54763
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Conjugated linoleic acid is a preferential substrate for fatty acid nitrationBonacci, Gustavo RobertoBaker, Paul R. S.Salvatore, Sonia RosanaShores, DarlaKhoo, Nicholas K. H.Koenitzer, Jeffrey R.Vitturi, Dario A.Woodcock, Steven R.Golin-Bisello, FrancaCole, Marsha P.Watkins, SimonSt. Croix, ClaudetteBatthyany, Carlos I.Freeman, Bruce A.Schopfer, Francisco J.Fatty Acid NitrationConjugated Linoleic AcidNitrationNitritehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The oxidation and nitration of unsaturated fatty acids by oxides of nitrogen yield electrophilic derivatives that can modulate protein function via post-translational protein modifications. The biological mechanisms accounting for fatty acid nitration and the specific structural characteristics of products remain to be defined. Herein, conjugated linoleic acid (CLA) is identified as the primary endogenous substrate for fatty acid nitration in vitro and in vivo, yielding up to 105 greater extent of nitration products as compared with bis-allylic linoleic acid. Multiple enzymatic and cellular mechanisms account for CLA nitration, including reactions catalyzed by mitochondria, activated macrophages, and gastric acidification. Nitroalkene derivatives of CLA and their metabolites are detected in the plasma of healthy humans and are increased in tissues undergoing episodes of ischemia reperfusion. Dietary CLA and nitrite supplementation in rodents elevates NO2-CLA levels in plasma, urine, and tissues, which in turn induces heme oxygenase-1 (HO-1) expression in the colonic epithelium. These results affirm that metabolic and inflammatory reactions yield electrophilic products that can modulate adaptive cell signaling mechanisms.Fil: Bonacci, Gustavo Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados UnidosFil: Baker, Paul R. S.. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados UnidosFil: Salvatore, Sonia Rosana. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados UnidosFil: Shores, Darla. University of Pittsburgh; Estados UnidosFil: Khoo, Nicholas K. H.. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados UnidosFil: Koenitzer, Jeffrey R.. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados UnidosFil: Vitturi, Dario A.. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados UnidosFil: Woodcock, Steven R.. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados UnidosFil: Golin-Bisello, Franca. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados UnidosFil: Cole, Marsha P.. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados UnidosFil: Watkins, Simon. University of Pittsburgh; Estados UnidosFil: St. Croix, Claudette. University of Pittsburgh; Estados UnidosFil: Batthyany, Carlos I.. Instituto Pasteur; Uruguay. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados UnidosFil: Freeman, Bruce A.. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados UnidosFil: Schopfer, Francisco J.. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados UnidosAmerican Society for Biochemistry and Molecular Biology2012-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54763Bonacci, Gustavo Roberto; Baker, Paul R. S.; Salvatore, Sonia Rosana; Shores, Darla; Khoo, Nicholas K. H.; et al.; Conjugated linoleic acid is a preferential substrate for fatty acid nitration; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 287; 53; 12-2012; 44071-440820021-92581083-351XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.jbc.org/content/287/53/44071info:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.M112.401356info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:54Zoai:ri.conicet.gov.ar:11336/54763instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:54.478CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Conjugated linoleic acid is a preferential substrate for fatty acid nitration
title Conjugated linoleic acid is a preferential substrate for fatty acid nitration
spellingShingle Conjugated linoleic acid is a preferential substrate for fatty acid nitration
Bonacci, Gustavo Roberto
Fatty Acid Nitration
Conjugated Linoleic Acid
Nitration
Nitrite
title_short Conjugated linoleic acid is a preferential substrate for fatty acid nitration
title_full Conjugated linoleic acid is a preferential substrate for fatty acid nitration
title_fullStr Conjugated linoleic acid is a preferential substrate for fatty acid nitration
title_full_unstemmed Conjugated linoleic acid is a preferential substrate for fatty acid nitration
title_sort Conjugated linoleic acid is a preferential substrate for fatty acid nitration
dc.creator.none.fl_str_mv Bonacci, Gustavo Roberto
Baker, Paul R. S.
Salvatore, Sonia Rosana
Shores, Darla
Khoo, Nicholas K. H.
Koenitzer, Jeffrey R.
Vitturi, Dario A.
Woodcock, Steven R.
Golin-Bisello, Franca
Cole, Marsha P.
Watkins, Simon
St. Croix, Claudette
Batthyany, Carlos I.
Freeman, Bruce A.
Schopfer, Francisco J.
author Bonacci, Gustavo Roberto
author_facet Bonacci, Gustavo Roberto
Baker, Paul R. S.
Salvatore, Sonia Rosana
Shores, Darla
Khoo, Nicholas K. H.
Koenitzer, Jeffrey R.
Vitturi, Dario A.
Woodcock, Steven R.
Golin-Bisello, Franca
Cole, Marsha P.
Watkins, Simon
St. Croix, Claudette
Batthyany, Carlos I.
Freeman, Bruce A.
Schopfer, Francisco J.
author_role author
author2 Baker, Paul R. S.
Salvatore, Sonia Rosana
Shores, Darla
Khoo, Nicholas K. H.
Koenitzer, Jeffrey R.
Vitturi, Dario A.
Woodcock, Steven R.
Golin-Bisello, Franca
Cole, Marsha P.
Watkins, Simon
St. Croix, Claudette
Batthyany, Carlos I.
Freeman, Bruce A.
Schopfer, Francisco J.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Fatty Acid Nitration
Conjugated Linoleic Acid
Nitration
Nitrite
topic Fatty Acid Nitration
Conjugated Linoleic Acid
Nitration
Nitrite
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The oxidation and nitration of unsaturated fatty acids by oxides of nitrogen yield electrophilic derivatives that can modulate protein function via post-translational protein modifications. The biological mechanisms accounting for fatty acid nitration and the specific structural characteristics of products remain to be defined. Herein, conjugated linoleic acid (CLA) is identified as the primary endogenous substrate for fatty acid nitration in vitro and in vivo, yielding up to 105 greater extent of nitration products as compared with bis-allylic linoleic acid. Multiple enzymatic and cellular mechanisms account for CLA nitration, including reactions catalyzed by mitochondria, activated macrophages, and gastric acidification. Nitroalkene derivatives of CLA and their metabolites are detected in the plasma of healthy humans and are increased in tissues undergoing episodes of ischemia reperfusion. Dietary CLA and nitrite supplementation in rodents elevates NO2-CLA levels in plasma, urine, and tissues, which in turn induces heme oxygenase-1 (HO-1) expression in the colonic epithelium. These results affirm that metabolic and inflammatory reactions yield electrophilic products that can modulate adaptive cell signaling mechanisms.
Fil: Bonacci, Gustavo Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados Unidos
Fil: Baker, Paul R. S.. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados Unidos
Fil: Salvatore, Sonia Rosana. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados Unidos
Fil: Shores, Darla. University of Pittsburgh; Estados Unidos
Fil: Khoo, Nicholas K. H.. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados Unidos
Fil: Koenitzer, Jeffrey R.. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados Unidos
Fil: Vitturi, Dario A.. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados Unidos
Fil: Woodcock, Steven R.. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados Unidos
Fil: Golin-Bisello, Franca. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados Unidos
Fil: Cole, Marsha P.. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados Unidos
Fil: Watkins, Simon. University of Pittsburgh; Estados Unidos
Fil: St. Croix, Claudette. University of Pittsburgh; Estados Unidos
Fil: Batthyany, Carlos I.. Instituto Pasteur; Uruguay. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados Unidos
Fil: Freeman, Bruce A.. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados Unidos
Fil: Schopfer, Francisco J.. Univeristy Of Pittsburgh. School Of Medicine. Department Of Pharmacology And Chemical Biology; Estados Unidos
description The oxidation and nitration of unsaturated fatty acids by oxides of nitrogen yield electrophilic derivatives that can modulate protein function via post-translational protein modifications. The biological mechanisms accounting for fatty acid nitration and the specific structural characteristics of products remain to be defined. Herein, conjugated linoleic acid (CLA) is identified as the primary endogenous substrate for fatty acid nitration in vitro and in vivo, yielding up to 105 greater extent of nitration products as compared with bis-allylic linoleic acid. Multiple enzymatic and cellular mechanisms account for CLA nitration, including reactions catalyzed by mitochondria, activated macrophages, and gastric acidification. Nitroalkene derivatives of CLA and their metabolites are detected in the plasma of healthy humans and are increased in tissues undergoing episodes of ischemia reperfusion. Dietary CLA and nitrite supplementation in rodents elevates NO2-CLA levels in plasma, urine, and tissues, which in turn induces heme oxygenase-1 (HO-1) expression in the colonic epithelium. These results affirm that metabolic and inflammatory reactions yield electrophilic products that can modulate adaptive cell signaling mechanisms.
publishDate 2012
dc.date.none.fl_str_mv 2012-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54763
Bonacci, Gustavo Roberto; Baker, Paul R. S.; Salvatore, Sonia Rosana; Shores, Darla; Khoo, Nicholas K. H.; et al.; Conjugated linoleic acid is a preferential substrate for fatty acid nitration; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 287; 53; 12-2012; 44071-44082
0021-9258
1083-351X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54763
identifier_str_mv Bonacci, Gustavo Roberto; Baker, Paul R. S.; Salvatore, Sonia Rosana; Shores, Darla; Khoo, Nicholas K. H.; et al.; Conjugated linoleic acid is a preferential substrate for fatty acid nitration; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 287; 53; 12-2012; 44071-44082
0021-9258
1083-351X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.jbc.org/content/287/53/44071
info:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.M112.401356
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269255884603392
score 13.13397

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