Biological Effects of Trace Elements on Lateralized Exploratory Activity, Defensive Behaviour, and Epigenetic DNA Molecular Changes in Maturing Rats

Autores
Ratti, Silvia Gabriela; Vizioli, Nora Matilde; Gaglio, Eliana; Alvarez Toro, Edgardo Orozimbo
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In a previous study, children of a determined geographical zone, characterized by the abundance of mineral deposits in La Rioja, province of Argentina, were found to have altered phenotypic expression attributed to the HSR gene. This gene has been found to be associated to handedness, brain asymmetry, reading-writing abilities and susceptibility to schizophrenia. A hypothesis was raised considering the epigenetic regulation of the HSR gene and its susceptibility to environmental influences; trace elements abundant in this region could be exogenous factors involved in the altered expression attributed to this gene. Thus, the objective of the present work was to test in a cognitive lateralization and associate behavioural responses rat model, the possible biological effect of ZnTe as representative trace element on some spontaneous and natural behavioural responses simile to HSR expression of humans. ZnTe treatment (0.03 µg/L - 3 µg/L) was applied in drinking water to pregnant mother animals along all gestation, delivery, weaning and preadolescent periods. Results showed that ZnTe treatment produced two opposing effects. On one hand, treated young rats displayed excitatory motor and selective motivated exploration responses in a behavioural automatic activity device, and on the other hand, impairments in motivated and lateralized behavioural display in a lateralized exploratory labyrinth. In other different test measuring defensive behaviour, natural defensive responses were attenuated in ZnTe treated rats. Biochemical determinations of the methylation patterns of DNA in prefrontal cortex and hippocampus, showed that ZnTe treatment modified the ratio of non-methylated- to methylated cytosine, suggesting an epigenetic change on the same line to that observed in children in the previous study of this laboratory. In conclusion, the behavioural rat model used in the present study confirms some of the previous evidence found in humans.
Fil: Ratti, Silvia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Vizioli, Nora Matilde. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina
Fil: Gaglio, Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Alvarez Toro, Edgardo Orozimbo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Materia
Defensive Behaviour
Trace Elements
Methyl Cytosine
Epigenetic Changes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/67670
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/67670
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biological Effects of Trace Elements on Lateralized Exploratory Activity, Defensive Behaviour, and Epigenetic DNA Molecular Changes in Maturing RatsRatti, Silvia GabrielaVizioli, Nora MatildeGaglio, ElianaAlvarez Toro, Edgardo OrozimboDefensive BehaviourTrace ElementsMethyl CytosineEpigenetic Changeshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3In a previous study, children of a determined geographical zone, characterized by the abundance of mineral deposits in La Rioja, province of Argentina, were found to have altered phenotypic expression attributed to the HSR gene. This gene has been found to be associated to handedness, brain asymmetry, reading-writing abilities and susceptibility to schizophrenia. A hypothesis was raised considering the epigenetic regulation of the HSR gene and its susceptibility to environmental influences; trace elements abundant in this region could be exogenous factors involved in the altered expression attributed to this gene. Thus, the objective of the present work was to test in a cognitive lateralization and associate behavioural responses rat model, the possible biological effect of ZnTe as representative trace element on some spontaneous and natural behavioural responses simile to HSR expression of humans. ZnTe treatment (0.03 µg/L - 3 µg/L) was applied in drinking water to pregnant mother animals along all gestation, delivery, weaning and preadolescent periods. Results showed that ZnTe treatment produced two opposing effects. On one hand, treated young rats displayed excitatory motor and selective motivated exploration responses in a behavioural automatic activity device, and on the other hand, impairments in motivated and lateralized behavioural display in a lateralized exploratory labyrinth. In other different test measuring defensive behaviour, natural defensive responses were attenuated in ZnTe treated rats. Biochemical determinations of the methylation patterns of DNA in prefrontal cortex and hippocampus, showed that ZnTe treatment modified the ratio of non-methylated- to methylated cytosine, suggesting an epigenetic change on the same line to that observed in children in the previous study of this laboratory. In conclusion, the behavioural rat model used in the present study confirms some of the previous evidence found in humans.Fil: Ratti, Silvia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Vizioli, Nora Matilde. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; ArgentinaFil: Gaglio, Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Alvarez Toro, Edgardo Orozimbo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaAmerican Scientific Publishers2012-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/67670Ratti, Silvia Gabriela; Vizioli, Nora Matilde; Gaglio, Eliana; Alvarez Toro, Edgardo Orozimbo; Biological Effects of Trace Elements on Lateralized Exploratory Activity, Defensive Behaviour, and Epigenetic DNA Molecular Changes in Maturing Rats; American Scientific Publishers; American Journal of Neuroprotection and Neuroregeneration; 4; 2; 12-2012; 167-1751947-2951CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1166/ajnn.2012.1047info:eu-repo/semantics/altIdentifier/url/https://www.ingentaconnect.com/content/asp/ajnn/2012/00000004/00000002/art00009info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:00:56Zoai:ri.conicet.gov.ar:11336/67670instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:00:56.458CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biological Effects of Trace Elements on Lateralized Exploratory Activity, Defensive Behaviour, and Epigenetic DNA Molecular Changes in Maturing Rats
title Biological Effects of Trace Elements on Lateralized Exploratory Activity, Defensive Behaviour, and Epigenetic DNA Molecular Changes in Maturing Rats
spellingShingle Biological Effects of Trace Elements on Lateralized Exploratory Activity, Defensive Behaviour, and Epigenetic DNA Molecular Changes in Maturing Rats
Ratti, Silvia Gabriela
Defensive Behaviour
Trace Elements
Methyl Cytosine
Epigenetic Changes
title_short Biological Effects of Trace Elements on Lateralized Exploratory Activity, Defensive Behaviour, and Epigenetic DNA Molecular Changes in Maturing Rats
title_full Biological Effects of Trace Elements on Lateralized Exploratory Activity, Defensive Behaviour, and Epigenetic DNA Molecular Changes in Maturing Rats
title_fullStr Biological Effects of Trace Elements on Lateralized Exploratory Activity, Defensive Behaviour, and Epigenetic DNA Molecular Changes in Maturing Rats
title_full_unstemmed Biological Effects of Trace Elements on Lateralized Exploratory Activity, Defensive Behaviour, and Epigenetic DNA Molecular Changes in Maturing Rats
title_sort Biological Effects of Trace Elements on Lateralized Exploratory Activity, Defensive Behaviour, and Epigenetic DNA Molecular Changes in Maturing Rats
dc.creator.none.fl_str_mv Ratti, Silvia Gabriela
Vizioli, Nora Matilde
Gaglio, Eliana
Alvarez Toro, Edgardo Orozimbo
author Ratti, Silvia Gabriela
author_facet Ratti, Silvia Gabriela
Vizioli, Nora Matilde
Gaglio, Eliana
Alvarez Toro, Edgardo Orozimbo
author_role author
author2 Vizioli, Nora Matilde
Gaglio, Eliana
Alvarez Toro, Edgardo Orozimbo
author2_role author
author
author
dc.subject.none.fl_str_mv Defensive Behaviour
Trace Elements
Methyl Cytosine
Epigenetic Changes
topic Defensive Behaviour
Trace Elements
Methyl Cytosine
Epigenetic Changes
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In a previous study, children of a determined geographical zone, characterized by the abundance of mineral deposits in La Rioja, province of Argentina, were found to have altered phenotypic expression attributed to the HSR gene. This gene has been found to be associated to handedness, brain asymmetry, reading-writing abilities and susceptibility to schizophrenia. A hypothesis was raised considering the epigenetic regulation of the HSR gene and its susceptibility to environmental influences; trace elements abundant in this region could be exogenous factors involved in the altered expression attributed to this gene. Thus, the objective of the present work was to test in a cognitive lateralization and associate behavioural responses rat model, the possible biological effect of ZnTe as representative trace element on some spontaneous and natural behavioural responses simile to HSR expression of humans. ZnTe treatment (0.03 µg/L - 3 µg/L) was applied in drinking water to pregnant mother animals along all gestation, delivery, weaning and preadolescent periods. Results showed that ZnTe treatment produced two opposing effects. On one hand, treated young rats displayed excitatory motor and selective motivated exploration responses in a behavioural automatic activity device, and on the other hand, impairments in motivated and lateralized behavioural display in a lateralized exploratory labyrinth. In other different test measuring defensive behaviour, natural defensive responses were attenuated in ZnTe treated rats. Biochemical determinations of the methylation patterns of DNA in prefrontal cortex and hippocampus, showed that ZnTe treatment modified the ratio of non-methylated- to methylated cytosine, suggesting an epigenetic change on the same line to that observed in children in the previous study of this laboratory. In conclusion, the behavioural rat model used in the present study confirms some of the previous evidence found in humans.
Fil: Ratti, Silvia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Vizioli, Nora Matilde. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina
Fil: Gaglio, Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Alvarez Toro, Edgardo Orozimbo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
description In a previous study, children of a determined geographical zone, characterized by the abundance of mineral deposits in La Rioja, province of Argentina, were found to have altered phenotypic expression attributed to the HSR gene. This gene has been found to be associated to handedness, brain asymmetry, reading-writing abilities and susceptibility to schizophrenia. A hypothesis was raised considering the epigenetic regulation of the HSR gene and its susceptibility to environmental influences; trace elements abundant in this region could be exogenous factors involved in the altered expression attributed to this gene. Thus, the objective of the present work was to test in a cognitive lateralization and associate behavioural responses rat model, the possible biological effect of ZnTe as representative trace element on some spontaneous and natural behavioural responses simile to HSR expression of humans. ZnTe treatment (0.03 µg/L - 3 µg/L) was applied in drinking water to pregnant mother animals along all gestation, delivery, weaning and preadolescent periods. Results showed that ZnTe treatment produced two opposing effects. On one hand, treated young rats displayed excitatory motor and selective motivated exploration responses in a behavioural automatic activity device, and on the other hand, impairments in motivated and lateralized behavioural display in a lateralized exploratory labyrinth. In other different test measuring defensive behaviour, natural defensive responses were attenuated in ZnTe treated rats. Biochemical determinations of the methylation patterns of DNA in prefrontal cortex and hippocampus, showed that ZnTe treatment modified the ratio of non-methylated- to methylated cytosine, suggesting an epigenetic change on the same line to that observed in children in the previous study of this laboratory. In conclusion, the behavioural rat model used in the present study confirms some of the previous evidence found in humans.
publishDate 2012
dc.date.none.fl_str_mv 2012-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/67670
Ratti, Silvia Gabriela; Vizioli, Nora Matilde; Gaglio, Eliana; Alvarez Toro, Edgardo Orozimbo; Biological Effects of Trace Elements on Lateralized Exploratory Activity, Defensive Behaviour, and Epigenetic DNA Molecular Changes in Maturing Rats; American Scientific Publishers; American Journal of Neuroprotection and Neuroregeneration; 4; 2; 12-2012; 167-175
1947-2951
CONICET Digital
CONICET
url http://hdl.handle.net/11336/67670
identifier_str_mv Ratti, Silvia Gabriela; Vizioli, Nora Matilde; Gaglio, Eliana; Alvarez Toro, Edgardo Orozimbo; Biological Effects of Trace Elements on Lateralized Exploratory Activity, Defensive Behaviour, and Epigenetic DNA Molecular Changes in Maturing Rats; American Scientific Publishers; American Journal of Neuroprotection and Neuroregeneration; 4; 2; 12-2012; 167-175
1947-2951
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1166/ajnn.2012.1047
info:eu-repo/semantics/altIdentifier/url/https://www.ingentaconnect.com/content/asp/ajnn/2012/00000004/00000002/art00009
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Scientific Publishers
publisher.none.fl_str_mv American Scientific Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.48226

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