Epigenetic modulation expressed as methylation changes in DNA from primary school children of two different geographical environments II

Autores
Ratti, Silvia Gabriela; Vizioli, Nora Matilde; Alvarez, Edgardo Oscar
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In previous work, proportions of the phenotypic characteristics attributable to the HSR (Hand Skill Relative, OMIM 139900) gene were found modified in primary school children from one of two distinct geographical regions of La Rioja province (Argentina). HSR gene has been described subjected to epigenetic regulation, and one of the main molecular mechanisms whereby this regulation is performed is by methylation of DNA. Thus, it was of interest in the present work to analyze in these children the possibility of changes in the DNA methylation patterns. 40 children from the two regions (20 from Region 2, and 20 from Region 1, considered control) were randomly selected from the original total sample of 547 children, and blood samples were taken to analyze the DNA methylation patterns by capillary electrophoresis technique. The phenotypic characterization for HSR also was performed on the children sample. Results showed that children from Region 2 had significantly altered the writing/reading capacity with a proportion about 65% of writing disturbances over control children of Region 1. No statistical differences were found in the other two phenotypic traits linked to the HSR gene (brain asymmetry and handedness). Molecular analysis of the methylation patterns revealed a significantly higher ratio of non-methylated to methylated cytosine in DNA from children of Region 2, compared to those of Region 1 (control). The present results suggest that the altered proportions of phenotypic characteristics found in children of Region 2 are supported by altered DNA methylation patterns, suggesting an environmental epigenetic modulation of DNA expression.
Fil: Ratti, Silvia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Fundación H. Barceló; Argentina. Universidad Nacional de Cuyo; Argentina
Fil: Vizioli, Nora Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Alvarez, Edgardo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo; Argentina
Materia
Trace Elements
Epigenesis
Methylation Patterns
Dna
Hrs
Cytosine/Methyl Cytosine Ratio
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/18239
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/18239
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Epigenetic modulation expressed as methylation changes in DNA from primary school children of two different geographical environments IIRatti, Silvia GabrielaVizioli, Nora MatildeAlvarez, Edgardo OscarTrace ElementsEpigenesisMethylation PatternsDnaHrsCytosine/Methyl Cytosine Ratiohttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3In previous work, proportions of the phenotypic characteristics attributable to the HSR (Hand Skill Relative, OMIM 139900) gene were found modified in primary school children from one of two distinct geographical regions of La Rioja province (Argentina). HSR gene has been described subjected to epigenetic regulation, and one of the main molecular mechanisms whereby this regulation is performed is by methylation of DNA. Thus, it was of interest in the present work to analyze in these children the possibility of changes in the DNA methylation patterns. 40 children from the two regions (20 from Region 2, and 20 from Region 1, considered control) were randomly selected from the original total sample of 547 children, and blood samples were taken to analyze the DNA methylation patterns by capillary electrophoresis technique. The phenotypic characterization for HSR also was performed on the children sample. Results showed that children from Region 2 had significantly altered the writing/reading capacity with a proportion about 65% of writing disturbances over control children of Region 1. No statistical differences were found in the other two phenotypic traits linked to the HSR gene (brain asymmetry and handedness). Molecular analysis of the methylation patterns revealed a significantly higher ratio of non-methylated to methylated cytosine in DNA from children of Region 2, compared to those of Region 1 (control). The present results suggest that the altered proportions of phenotypic characteristics found in children of Region 2 are supported by altered DNA methylation patterns, suggesting an environmental epigenetic modulation of DNA expression.Fil: Ratti, Silvia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Fundación H. Barceló; Argentina. Universidad Nacional de Cuyo; ArgentinaFil: Vizioli, Nora Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Alvarez, Edgardo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo; ArgentinaAmerican Scientific Publishers2010-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/18239Ratti, Silvia Gabriela; Vizioli, Nora Matilde; Alvarez, Edgardo Oscar; Epigenetic modulation expressed as methylation changes in DNA from primary school children of two different geographical environments II; American Scientific Publishers; American Journal of Neuroprotection and Neuroregeneration; 2; 1; 6-2010; 65-701947-29511947-296XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1166/ajnn.2010.1014info:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/content/asp/ajnn/2010/00000002/00000001/art00008?token=004d1d0cc98f808e70a646e5865462431636f3b6b35217e6b6a775c5e4e6b634014e594efe018info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:57Zoai:ri.conicet.gov.ar:11336/18239instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:58.016CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Epigenetic modulation expressed as methylation changes in DNA from primary school children of two different geographical environments II
title Epigenetic modulation expressed as methylation changes in DNA from primary school children of two different geographical environments II
spellingShingle Epigenetic modulation expressed as methylation changes in DNA from primary school children of two different geographical environments II
Ratti, Silvia Gabriela
Trace Elements
Epigenesis
Methylation Patterns
Dna
Hrs
Cytosine/Methyl Cytosine Ratio
title_short Epigenetic modulation expressed as methylation changes in DNA from primary school children of two different geographical environments II
title_full Epigenetic modulation expressed as methylation changes in DNA from primary school children of two different geographical environments II
title_fullStr Epigenetic modulation expressed as methylation changes in DNA from primary school children of two different geographical environments II
title_full_unstemmed Epigenetic modulation expressed as methylation changes in DNA from primary school children of two different geographical environments II
title_sort Epigenetic modulation expressed as methylation changes in DNA from primary school children of two different geographical environments II
dc.creator.none.fl_str_mv Ratti, Silvia Gabriela
Vizioli, Nora Matilde
Alvarez, Edgardo Oscar
author Ratti, Silvia Gabriela
author_facet Ratti, Silvia Gabriela
Vizioli, Nora Matilde
Alvarez, Edgardo Oscar
author_role author
author2 Vizioli, Nora Matilde
Alvarez, Edgardo Oscar
author2_role author
author
dc.subject.none.fl_str_mv Trace Elements
Epigenesis
Methylation Patterns
Dna
Hrs
Cytosine/Methyl Cytosine Ratio
topic Trace Elements
Epigenesis
Methylation Patterns
Dna
Hrs
Cytosine/Methyl Cytosine Ratio
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In previous work, proportions of the phenotypic characteristics attributable to the HSR (Hand Skill Relative, OMIM 139900) gene were found modified in primary school children from one of two distinct geographical regions of La Rioja province (Argentina). HSR gene has been described subjected to epigenetic regulation, and one of the main molecular mechanisms whereby this regulation is performed is by methylation of DNA. Thus, it was of interest in the present work to analyze in these children the possibility of changes in the DNA methylation patterns. 40 children from the two regions (20 from Region 2, and 20 from Region 1, considered control) were randomly selected from the original total sample of 547 children, and blood samples were taken to analyze the DNA methylation patterns by capillary electrophoresis technique. The phenotypic characterization for HSR also was performed on the children sample. Results showed that children from Region 2 had significantly altered the writing/reading capacity with a proportion about 65% of writing disturbances over control children of Region 1. No statistical differences were found in the other two phenotypic traits linked to the HSR gene (brain asymmetry and handedness). Molecular analysis of the methylation patterns revealed a significantly higher ratio of non-methylated to methylated cytosine in DNA from children of Region 2, compared to those of Region 1 (control). The present results suggest that the altered proportions of phenotypic characteristics found in children of Region 2 are supported by altered DNA methylation patterns, suggesting an environmental epigenetic modulation of DNA expression.
Fil: Ratti, Silvia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Fundación H. Barceló; Argentina. Universidad Nacional de Cuyo; Argentina
Fil: Vizioli, Nora Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Alvarez, Edgardo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo; Argentina
description In previous work, proportions of the phenotypic characteristics attributable to the HSR (Hand Skill Relative, OMIM 139900) gene were found modified in primary school children from one of two distinct geographical regions of La Rioja province (Argentina). HSR gene has been described subjected to epigenetic regulation, and one of the main molecular mechanisms whereby this regulation is performed is by methylation of DNA. Thus, it was of interest in the present work to analyze in these children the possibility of changes in the DNA methylation patterns. 40 children from the two regions (20 from Region 2, and 20 from Region 1, considered control) were randomly selected from the original total sample of 547 children, and blood samples were taken to analyze the DNA methylation patterns by capillary electrophoresis technique. The phenotypic characterization for HSR also was performed on the children sample. Results showed that children from Region 2 had significantly altered the writing/reading capacity with a proportion about 65% of writing disturbances over control children of Region 1. No statistical differences were found in the other two phenotypic traits linked to the HSR gene (brain asymmetry and handedness). Molecular analysis of the methylation patterns revealed a significantly higher ratio of non-methylated to methylated cytosine in DNA from children of Region 2, compared to those of Region 1 (control). The present results suggest that the altered proportions of phenotypic characteristics found in children of Region 2 are supported by altered DNA methylation patterns, suggesting an environmental epigenetic modulation of DNA expression.
publishDate 2010
dc.date.none.fl_str_mv 2010-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/18239
Ratti, Silvia Gabriela; Vizioli, Nora Matilde; Alvarez, Edgardo Oscar; Epigenetic modulation expressed as methylation changes in DNA from primary school children of two different geographical environments II; American Scientific Publishers; American Journal of Neuroprotection and Neuroregeneration; 2; 1; 6-2010; 65-70
1947-2951
1947-296X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/18239
identifier_str_mv Ratti, Silvia Gabriela; Vizioli, Nora Matilde; Alvarez, Edgardo Oscar; Epigenetic modulation expressed as methylation changes in DNA from primary school children of two different geographical environments II; American Scientific Publishers; American Journal of Neuroprotection and Neuroregeneration; 2; 1; 6-2010; 65-70
1947-2951
1947-296X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1166/ajnn.2010.1014
info:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/content/asp/ajnn/2010/00000002/00000001/art00008?token=004d1d0cc98f808e70a646e5865462431636f3b6b35217e6b6a775c5e4e6b634014e594efe018
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Scientific Publishers
publisher.none.fl_str_mv American Scientific Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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