Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice
- Autores
- Bonell, Martha Lucrecia; Arguissain, Gustavo Gabriel; Crepy, Maria Andrea; Fernandez, Paula del Carmen; Heinz, Ruth Amelia
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In Argentina, the yield potential of rice (Oryza sativa L.) under suboptimal temperatures of temperate regions and during early sowing in tropical regions depends on its low-temperature germinability (LTG) and subsequent cold tolerance at the seedling stage (CTS). This study focused on the phenotyping and identification of contrasting henotypes for LTG and CTS of 18 rice genotypes genotyped with 49 markers previously linked to cold tolerance. The selected markers revealed phenotypic variability among the genotypes evaluated. The results also demonstrated that both traits are regulated by different genes and that CTS is associated with differential alleles of candidate gene (CG)-based markers. Biplot clustering analysis allowed the selection of two cultivars, H298a/90 and Puitá INTA CL, contrasting for LTG, CTS and CG alleles. An F2 population (n = 128) derived from these two cultivars was genotyped with 43 SSR- and CG-based markers and 89 F2:3 families (~ 1800 seedlings) phenotyped for CTS. Single-marker analysis detected two major QTL that had been previously reported for CTS: qCTS11, which explained 18.35% of the phenotypic variance at 7 days of treatment, and qCTS12, which explained 26.62% and 47.94% of the phenotypic variance at 7 and 14 days of treatment, respectively. Marker-assisted introgression of QTL alleles favorable for CTS into Puitá INTA CL, which has high LTG, could contribute to obtaining breeding lines with early seedling vigor adapted to the sowing season in Argentina.
Fil: Bonell, Martha Lucrecia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Arguissain, Gustavo Gabriel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Crepy, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernandez, Paula del Carmen. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina - Materia
-
CANDIDATE GENES
COLD TOLERANCE (CT)
COLD TOLERANCE AT SEEDLING STAGE (CTS)
LOW-TEMPERATURE GERMINATION ABILITY (LTG)
MARKER-ASSISTED SELECTION (MAS) - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/142403
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/142403 |
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3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate riceBonell, Martha LucreciaArguissain, Gustavo GabrielCrepy, Maria AndreaFernandez, Paula del CarmenHeinz, Ruth AmeliaCANDIDATE GENESCOLD TOLERANCE (CT)COLD TOLERANCE AT SEEDLING STAGE (CTS)LOW-TEMPERATURE GERMINATION ABILITY (LTG)MARKER-ASSISTED SELECTION (MAS)https://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4In Argentina, the yield potential of rice (Oryza sativa L.) under suboptimal temperatures of temperate regions and during early sowing in tropical regions depends on its low-temperature germinability (LTG) and subsequent cold tolerance at the seedling stage (CTS). This study focused on the phenotyping and identification of contrasting henotypes for LTG and CTS of 18 rice genotypes genotyped with 49 markers previously linked to cold tolerance. The selected markers revealed phenotypic variability among the genotypes evaluated. The results also demonstrated that both traits are regulated by different genes and that CTS is associated with differential alleles of candidate gene (CG)-based markers. Biplot clustering analysis allowed the selection of two cultivars, H298a/90 and Puitá INTA CL, contrasting for LTG, CTS and CG alleles. An F2 population (n = 128) derived from these two cultivars was genotyped with 43 SSR- and CG-based markers and 89 F2:3 families (~ 1800 seedlings) phenotyped for CTS. Single-marker analysis detected two major QTL that had been previously reported for CTS: qCTS11, which explained 18.35% of the phenotypic variance at 7 days of treatment, and qCTS12, which explained 26.62% and 47.94% of the phenotypic variance at 7 and 14 days of treatment, respectively. Marker-assisted introgression of QTL alleles favorable for CTS into Puitá INTA CL, which has high LTG, could contribute to obtaining breeding lines with early seedling vigor adapted to the sowing season in Argentina.Fil: Bonell, Martha Lucrecia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Arguissain, Gustavo Gabriel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Crepy, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fernandez, Paula del Carmen. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaSpringer2020-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/142403Bonell, Martha Lucrecia; Arguissain, Gustavo Gabriel; Crepy, Maria Andrea; Fernandez, Paula del Carmen; Heinz, Ruth Amelia; Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice; Springer; Euphytica; 216; 8; 7-2020; 1-150014-2336CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s10681-020-02661-3info:eu-repo/semantics/altIdentifier/doi/10.1007/s10681-020-02661-3info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:47:13Zoai:ri.conicet.gov.ar:11336/142403instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:47:13.629CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice |
title |
Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice |
spellingShingle |
Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice Bonell, Martha Lucrecia CANDIDATE GENES COLD TOLERANCE (CT) COLD TOLERANCE AT SEEDLING STAGE (CTS) LOW-TEMPERATURE GERMINATION ABILITY (LTG) MARKER-ASSISTED SELECTION (MAS) |
title_short |
Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice |
title_full |
Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice |
title_fullStr |
Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice |
title_full_unstemmed |
Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice |
title_sort |
Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice |
dc.creator.none.fl_str_mv |
Bonell, Martha Lucrecia Arguissain, Gustavo Gabriel Crepy, Maria Andrea Fernandez, Paula del Carmen Heinz, Ruth Amelia |
author |
Bonell, Martha Lucrecia |
author_facet |
Bonell, Martha Lucrecia Arguissain, Gustavo Gabriel Crepy, Maria Andrea Fernandez, Paula del Carmen Heinz, Ruth Amelia |
author_role |
author |
author2 |
Arguissain, Gustavo Gabriel Crepy, Maria Andrea Fernandez, Paula del Carmen Heinz, Ruth Amelia |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
CANDIDATE GENES COLD TOLERANCE (CT) COLD TOLERANCE AT SEEDLING STAGE (CTS) LOW-TEMPERATURE GERMINATION ABILITY (LTG) MARKER-ASSISTED SELECTION (MAS) |
topic |
CANDIDATE GENES COLD TOLERANCE (CT) COLD TOLERANCE AT SEEDLING STAGE (CTS) LOW-TEMPERATURE GERMINATION ABILITY (LTG) MARKER-ASSISTED SELECTION (MAS) |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.4 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
In Argentina, the yield potential of rice (Oryza sativa L.) under suboptimal temperatures of temperate regions and during early sowing in tropical regions depends on its low-temperature germinability (LTG) and subsequent cold tolerance at the seedling stage (CTS). This study focused on the phenotyping and identification of contrasting henotypes for LTG and CTS of 18 rice genotypes genotyped with 49 markers previously linked to cold tolerance. The selected markers revealed phenotypic variability among the genotypes evaluated. The results also demonstrated that both traits are regulated by different genes and that CTS is associated with differential alleles of candidate gene (CG)-based markers. Biplot clustering analysis allowed the selection of two cultivars, H298a/90 and Puitá INTA CL, contrasting for LTG, CTS and CG alleles. An F2 population (n = 128) derived from these two cultivars was genotyped with 43 SSR- and CG-based markers and 89 F2:3 families (~ 1800 seedlings) phenotyped for CTS. Single-marker analysis detected two major QTL that had been previously reported for CTS: qCTS11, which explained 18.35% of the phenotypic variance at 7 days of treatment, and qCTS12, which explained 26.62% and 47.94% of the phenotypic variance at 7 and 14 days of treatment, respectively. Marker-assisted introgression of QTL alleles favorable for CTS into Puitá INTA CL, which has high LTG, could contribute to obtaining breeding lines with early seedling vigor adapted to the sowing season in Argentina. Fil: Bonell, Martha Lucrecia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Arguissain, Gustavo Gabriel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Crepy, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fernandez, Paula del Carmen. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina |
description |
In Argentina, the yield potential of rice (Oryza sativa L.) under suboptimal temperatures of temperate regions and during early sowing in tropical regions depends on its low-temperature germinability (LTG) and subsequent cold tolerance at the seedling stage (CTS). This study focused on the phenotyping and identification of contrasting henotypes for LTG and CTS of 18 rice genotypes genotyped with 49 markers previously linked to cold tolerance. The selected markers revealed phenotypic variability among the genotypes evaluated. The results also demonstrated that both traits are regulated by different genes and that CTS is associated with differential alleles of candidate gene (CG)-based markers. Biplot clustering analysis allowed the selection of two cultivars, H298a/90 and Puitá INTA CL, contrasting for LTG, CTS and CG alleles. An F2 population (n = 128) derived from these two cultivars was genotyped with 43 SSR- and CG-based markers and 89 F2:3 families (~ 1800 seedlings) phenotyped for CTS. Single-marker analysis detected two major QTL that had been previously reported for CTS: qCTS11, which explained 18.35% of the phenotypic variance at 7 days of treatment, and qCTS12, which explained 26.62% and 47.94% of the phenotypic variance at 7 and 14 days of treatment, respectively. Marker-assisted introgression of QTL alleles favorable for CTS into Puitá INTA CL, which has high LTG, could contribute to obtaining breeding lines with early seedling vigor adapted to the sowing season in Argentina. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/142403 Bonell, Martha Lucrecia; Arguissain, Gustavo Gabriel; Crepy, Maria Andrea; Fernandez, Paula del Carmen; Heinz, Ruth Amelia; Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice; Springer; Euphytica; 216; 8; 7-2020; 1-15 0014-2336 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/142403 |
identifier_str_mv |
Bonell, Martha Lucrecia; Arguissain, Gustavo Gabriel; Crepy, Maria Andrea; Fernandez, Paula del Carmen; Heinz, Ruth Amelia; Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice; Springer; Euphytica; 216; 8; 7-2020; 1-15 0014-2336 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s10681-020-02661-3 info:eu-repo/semantics/altIdentifier/doi/10.1007/s10681-020-02661-3 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614515545604096 |
score |
13.070432 |