Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice

Autores
Bonell, Martha Lucrecia; Arguissain, Gustavo Gabriel; Crepy, Maria Andrea; Fernandez, Paula del Carmen; Heinz, Ruth Amelia
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In Argentina, the yield potential of rice (Oryza sativa L.) under suboptimal temperatures of temperate regions and during early sowing in tropical regions depends on its low-temperature germinability (LTG) and subsequent cold tolerance at the seedling stage (CTS). This study focused on the phenotyping and identification of contrasting henotypes for LTG and CTS of 18 rice genotypes genotyped with 49 markers previously linked to cold tolerance. The selected markers revealed phenotypic variability among the genotypes evaluated. The results also demonstrated that both traits are regulated by different genes and that CTS is associated with differential alleles of candidate gene (CG)-based markers. Biplot clustering analysis allowed the selection of two cultivars, H298a/90 and Puitá INTA CL, contrasting for LTG, CTS and CG alleles. An F2 population (n = 128) derived from these two cultivars was genotyped with 43 SSR- and CG-based markers and 89 F2:3 families (~ 1800 seedlings) phenotyped for CTS. Single-marker analysis detected two major QTL that had been previously reported for CTS: qCTS11, which explained 18.35% of the phenotypic variance at 7 days of treatment, and qCTS12, which explained 26.62% and 47.94% of the phenotypic variance at 7 and 14 days of treatment, respectively. Marker-assisted introgression of QTL alleles favorable for CTS into Puitá INTA CL, which has high LTG, could contribute to obtaining breeding lines with early seedling vigor adapted to the sowing season in Argentina.
Fil: Bonell, Martha Lucrecia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Arguissain, Gustavo Gabriel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Crepy, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernandez, Paula del Carmen. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Materia
CANDIDATE GENES
COLD TOLERANCE (CT)
COLD TOLERANCE AT SEEDLING STAGE (CTS)
LOW-TEMPERATURE GERMINATION ABILITY (LTG)
MARKER-ASSISTED SELECTION (MAS)
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/142403
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/142403
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate riceBonell, Martha LucreciaArguissain, Gustavo GabrielCrepy, Maria AndreaFernandez, Paula del CarmenHeinz, Ruth AmeliaCANDIDATE GENESCOLD TOLERANCE (CT)COLD TOLERANCE AT SEEDLING STAGE (CTS)LOW-TEMPERATURE GERMINATION ABILITY (LTG)MARKER-ASSISTED SELECTION (MAS)https://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4In Argentina, the yield potential of rice (Oryza sativa L.) under suboptimal temperatures of temperate regions and during early sowing in tropical regions depends on its low-temperature germinability (LTG) and subsequent cold tolerance at the seedling stage (CTS). This study focused on the phenotyping and identification of contrasting henotypes for LTG and CTS of 18 rice genotypes genotyped with 49 markers previously linked to cold tolerance. The selected markers revealed phenotypic variability among the genotypes evaluated. The results also demonstrated that both traits are regulated by different genes and that CTS is associated with differential alleles of candidate gene (CG)-based markers. Biplot clustering analysis allowed the selection of two cultivars, H298a/90 and Puitá INTA CL, contrasting for LTG, CTS and CG alleles. An F2 population (n = 128) derived from these two cultivars was genotyped with 43 SSR- and CG-based markers and 89 F2:3 families (~ 1800 seedlings) phenotyped for CTS. Single-marker analysis detected two major QTL that had been previously reported for CTS: qCTS11, which explained 18.35% of the phenotypic variance at 7 days of treatment, and qCTS12, which explained 26.62% and 47.94% of the phenotypic variance at 7 and 14 days of treatment, respectively. Marker-assisted introgression of QTL alleles favorable for CTS into Puitá INTA CL, which has high LTG, could contribute to obtaining breeding lines with early seedling vigor adapted to the sowing season in Argentina.Fil: Bonell, Martha Lucrecia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Arguissain, Gustavo Gabriel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Crepy, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fernandez, Paula del Carmen. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaSpringer2020-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/142403Bonell, Martha Lucrecia; Arguissain, Gustavo Gabriel; Crepy, Maria Andrea; Fernandez, Paula del Carmen; Heinz, Ruth Amelia; Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice; Springer; Euphytica; 216; 8; 7-2020; 1-150014-2336CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s10681-020-02661-3info:eu-repo/semantics/altIdentifier/doi/10.1007/s10681-020-02661-3info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:20:38Zoai:ri.conicet.gov.ar:11336/142403instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:20:38.672CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice
title Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice
spellingShingle Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice
Bonell, Martha Lucrecia
CANDIDATE GENES
COLD TOLERANCE (CT)
COLD TOLERANCE AT SEEDLING STAGE (CTS)
LOW-TEMPERATURE GERMINATION ABILITY (LTG)
MARKER-ASSISTED SELECTION (MAS)
title_short Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice
title_full Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice
title_fullStr Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice
title_full_unstemmed Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice
title_sort Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice
dc.creator.none.fl_str_mv Bonell, Martha Lucrecia
Arguissain, Gustavo Gabriel
Crepy, Maria Andrea
Fernandez, Paula del Carmen
Heinz, Ruth Amelia
author Bonell, Martha Lucrecia
author_facet Bonell, Martha Lucrecia
Arguissain, Gustavo Gabriel
Crepy, Maria Andrea
Fernandez, Paula del Carmen
Heinz, Ruth Amelia
author_role author
author2 Arguissain, Gustavo Gabriel
Crepy, Maria Andrea
Fernandez, Paula del Carmen
Heinz, Ruth Amelia
author2_role author
author
author
author
dc.subject.none.fl_str_mv CANDIDATE GENES
COLD TOLERANCE (CT)
COLD TOLERANCE AT SEEDLING STAGE (CTS)
LOW-TEMPERATURE GERMINATION ABILITY (LTG)
MARKER-ASSISTED SELECTION (MAS)
topic CANDIDATE GENES
COLD TOLERANCE (CT)
COLD TOLERANCE AT SEEDLING STAGE (CTS)
LOW-TEMPERATURE GERMINATION ABILITY (LTG)
MARKER-ASSISTED SELECTION (MAS)
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv In Argentina, the yield potential of rice (Oryza sativa L.) under suboptimal temperatures of temperate regions and during early sowing in tropical regions depends on its low-temperature germinability (LTG) and subsequent cold tolerance at the seedling stage (CTS). This study focused on the phenotyping and identification of contrasting henotypes for LTG and CTS of 18 rice genotypes genotyped with 49 markers previously linked to cold tolerance. The selected markers revealed phenotypic variability among the genotypes evaluated. The results also demonstrated that both traits are regulated by different genes and that CTS is associated with differential alleles of candidate gene (CG)-based markers. Biplot clustering analysis allowed the selection of two cultivars, H298a/90 and Puitá INTA CL, contrasting for LTG, CTS and CG alleles. An F2 population (n = 128) derived from these two cultivars was genotyped with 43 SSR- and CG-based markers and 89 F2:3 families (~ 1800 seedlings) phenotyped for CTS. Single-marker analysis detected two major QTL that had been previously reported for CTS: qCTS11, which explained 18.35% of the phenotypic variance at 7 days of treatment, and qCTS12, which explained 26.62% and 47.94% of the phenotypic variance at 7 and 14 days of treatment, respectively. Marker-assisted introgression of QTL alleles favorable for CTS into Puitá INTA CL, which has high LTG, could contribute to obtaining breeding lines with early seedling vigor adapted to the sowing season in Argentina.
Fil: Bonell, Martha Lucrecia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Arguissain, Gustavo Gabriel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Crepy, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernandez, Paula del Carmen. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
description In Argentina, the yield potential of rice (Oryza sativa L.) under suboptimal temperatures of temperate regions and during early sowing in tropical regions depends on its low-temperature germinability (LTG) and subsequent cold tolerance at the seedling stage (CTS). This study focused on the phenotyping and identification of contrasting henotypes for LTG and CTS of 18 rice genotypes genotyped with 49 markers previously linked to cold tolerance. The selected markers revealed phenotypic variability among the genotypes evaluated. The results also demonstrated that both traits are regulated by different genes and that CTS is associated with differential alleles of candidate gene (CG)-based markers. Biplot clustering analysis allowed the selection of two cultivars, H298a/90 and Puitá INTA CL, contrasting for LTG, CTS and CG alleles. An F2 population (n = 128) derived from these two cultivars was genotyped with 43 SSR- and CG-based markers and 89 F2:3 families (~ 1800 seedlings) phenotyped for CTS. Single-marker analysis detected two major QTL that had been previously reported for CTS: qCTS11, which explained 18.35% of the phenotypic variance at 7 days of treatment, and qCTS12, which explained 26.62% and 47.94% of the phenotypic variance at 7 and 14 days of treatment, respectively. Marker-assisted introgression of QTL alleles favorable for CTS into Puitá INTA CL, which has high LTG, could contribute to obtaining breeding lines with early seedling vigor adapted to the sowing season in Argentina.
publishDate 2020
dc.date.none.fl_str_mv 2020-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/142403
Bonell, Martha Lucrecia; Arguissain, Gustavo Gabriel; Crepy, Maria Andrea; Fernandez, Paula del Carmen; Heinz, Ruth Amelia; Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice; Springer; Euphytica; 216; 8; 7-2020; 1-15
0014-2336
CONICET Digital
CONICET
url http://hdl.handle.net/11336/142403
identifier_str_mv Bonell, Martha Lucrecia; Arguissain, Gustavo Gabriel; Crepy, Maria Andrea; Fernandez, Paula del Carmen; Heinz, Ruth Amelia; Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice; Springer; Euphytica; 216; 8; 7-2020; 1-15
0014-2336
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s10681-020-02661-3
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10681-020-02661-3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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