Abscisic acid enhances cold tolerance in honeybee larvae

Autores
Ramírez, Leonor; Negri, Pedro; Sturla, Laura; Guida, Lucrezia; Vigliarolo, Tiziana; Maggi, Matías Daniel; Eguaras, Martin Javier; Zocchi, Elena; Lamattina, Lorenzo
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The natural composition of nutrients present in food is a key factor determining the immune function and stress responses in the honeybee (Apis mellifera). We previously demonstrated that a supplement of abscisic acid (ABA), a natural component of nectar, pollen, and honey, increases honeybee colony survival overwinter. Here we further explored the role of ABA in in vitro-reared larvae exposed to low temperatures. Four-day-old larvae (L4) exposed to 258C for 3 days showed lower survival rates and delayed development compared to individuals growing at a standard temperature (348C). Cold-stressed larvae maintained higher levels of ABA for longer than do larvae reared at 348C, suggesting a biological significance for ABA. Larvae fed with an ABA-supplemented diet completely prevent the low survival rate due to cold stress and accelerate adult emergence. ABA modulates the expression of genes involved in metabolic adjustments and stress responses: Hexamerin 70b, Insulin Receptor Substrate, Vitellogenin, and Heat Shock Proteins 70. AmLANCL2, the honeybee ABA receptor, is also regulated by cold stress and ABA. These results support a role for ABA increasing the tolerance of honeybee larvae to low temperatures through priming effects.
Fil: Ramírez, Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Negri, Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Sturla, Laura. Università degli Studi di Genova; Italia
Fil: Guida, Lucrezia. Università degli Studi di Genova; Italia
Fil: Vigliarolo, Tiziana. Università degli Studi di Genova; Italia
Fil: Maggi, Matías Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Eguaras, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Zocchi, Elena. Università degli Studi di Genova; Italia
Fil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Materia
ABSCISIC ACID
APIS MELLIFERA
COLD TOLERANCE
COMPENSATORY GROWTH
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/66704
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Abscisic acid enhances cold tolerance in honeybee larvaeRamírez, LeonorNegri, PedroSturla, LauraGuida, LucreziaVigliarolo, TizianaMaggi, Matías DanielEguaras, Martin JavierZocchi, ElenaLamattina, LorenzoABSCISIC ACIDAPIS MELLIFERACOLD TOLERANCECOMPENSATORY GROWTHhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The natural composition of nutrients present in food is a key factor determining the immune function and stress responses in the honeybee (Apis mellifera). We previously demonstrated that a supplement of abscisic acid (ABA), a natural component of nectar, pollen, and honey, increases honeybee colony survival overwinter. Here we further explored the role of ABA in in vitro-reared larvae exposed to low temperatures. Four-day-old larvae (L4) exposed to 258C for 3 days showed lower survival rates and delayed development compared to individuals growing at a standard temperature (348C). Cold-stressed larvae maintained higher levels of ABA for longer than do larvae reared at 348C, suggesting a biological significance for ABA. Larvae fed with an ABA-supplemented diet completely prevent the low survival rate due to cold stress and accelerate adult emergence. ABA modulates the expression of genes involved in metabolic adjustments and stress responses: Hexamerin 70b, Insulin Receptor Substrate, Vitellogenin, and Heat Shock Proteins 70. AmLANCL2, the honeybee ABA receptor, is also regulated by cold stress and ABA. These results support a role for ABA increasing the tolerance of honeybee larvae to low temperatures through priming effects.Fil: Ramírez, Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Negri, Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Sturla, Laura. Università degli Studi di Genova; ItaliaFil: Guida, Lucrezia. Università degli Studi di Genova; ItaliaFil: Vigliarolo, Tiziana. Università degli Studi di Genova; ItaliaFil: Maggi, Matías Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Eguaras, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Zocchi, Elena. Università degli Studi di Genova; ItaliaFil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaThe Royal Society2017-04-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/66704Ramírez, Leonor; Negri, Pedro; Sturla, Laura; Guida, Lucrezia; Vigliarolo, Tiziana; et al.; Abscisic acid enhances cold tolerance in honeybee larvae; The Royal Society; Proceedings of the Royal Society of London. Series B: Biological Sciences; 284; 1852; 5-4-2017; 1-90962-8452CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1098/rspb.2016.2140info:eu-repo/semantics/altIdentifier/url/https://royalsocietypublishing.org/doi/10.1098/rspb.2016.2140info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5394655/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:16:39Zoai:ri.conicet.gov.ar:11336/66704instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:16:39.289CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Abscisic acid enhances cold tolerance in honeybee larvae
title Abscisic acid enhances cold tolerance in honeybee larvae
spellingShingle Abscisic acid enhances cold tolerance in honeybee larvae
Ramírez, Leonor
ABSCISIC ACID
APIS MELLIFERA
COLD TOLERANCE
COMPENSATORY GROWTH
title_short Abscisic acid enhances cold tolerance in honeybee larvae
title_full Abscisic acid enhances cold tolerance in honeybee larvae
title_fullStr Abscisic acid enhances cold tolerance in honeybee larvae
title_full_unstemmed Abscisic acid enhances cold tolerance in honeybee larvae
title_sort Abscisic acid enhances cold tolerance in honeybee larvae
dc.creator.none.fl_str_mv Ramírez, Leonor
Negri, Pedro
Sturla, Laura
Guida, Lucrezia
Vigliarolo, Tiziana
Maggi, Matías Daniel
Eguaras, Martin Javier
Zocchi, Elena
Lamattina, Lorenzo
author Ramírez, Leonor
author_facet Ramírez, Leonor
Negri, Pedro
Sturla, Laura
Guida, Lucrezia
Vigliarolo, Tiziana
Maggi, Matías Daniel
Eguaras, Martin Javier
Zocchi, Elena
Lamattina, Lorenzo
author_role author
author2 Negri, Pedro
Sturla, Laura
Guida, Lucrezia
Vigliarolo, Tiziana
Maggi, Matías Daniel
Eguaras, Martin Javier
Zocchi, Elena
Lamattina, Lorenzo
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ABSCISIC ACID
APIS MELLIFERA
COLD TOLERANCE
COMPENSATORY GROWTH
topic ABSCISIC ACID
APIS MELLIFERA
COLD TOLERANCE
COMPENSATORY GROWTH
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The natural composition of nutrients present in food is a key factor determining the immune function and stress responses in the honeybee (Apis mellifera). We previously demonstrated that a supplement of abscisic acid (ABA), a natural component of nectar, pollen, and honey, increases honeybee colony survival overwinter. Here we further explored the role of ABA in in vitro-reared larvae exposed to low temperatures. Four-day-old larvae (L4) exposed to 258C for 3 days showed lower survival rates and delayed development compared to individuals growing at a standard temperature (348C). Cold-stressed larvae maintained higher levels of ABA for longer than do larvae reared at 348C, suggesting a biological significance for ABA. Larvae fed with an ABA-supplemented diet completely prevent the low survival rate due to cold stress and accelerate adult emergence. ABA modulates the expression of genes involved in metabolic adjustments and stress responses: Hexamerin 70b, Insulin Receptor Substrate, Vitellogenin, and Heat Shock Proteins 70. AmLANCL2, the honeybee ABA receptor, is also regulated by cold stress and ABA. These results support a role for ABA increasing the tolerance of honeybee larvae to low temperatures through priming effects.
Fil: Ramírez, Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Negri, Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Sturla, Laura. Università degli Studi di Genova; Italia
Fil: Guida, Lucrezia. Università degli Studi di Genova; Italia
Fil: Vigliarolo, Tiziana. Università degli Studi di Genova; Italia
Fil: Maggi, Matías Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Eguaras, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Zocchi, Elena. Università degli Studi di Genova; Italia
Fil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
description The natural composition of nutrients present in food is a key factor determining the immune function and stress responses in the honeybee (Apis mellifera). We previously demonstrated that a supplement of abscisic acid (ABA), a natural component of nectar, pollen, and honey, increases honeybee colony survival overwinter. Here we further explored the role of ABA in in vitro-reared larvae exposed to low temperatures. Four-day-old larvae (L4) exposed to 258C for 3 days showed lower survival rates and delayed development compared to individuals growing at a standard temperature (348C). Cold-stressed larvae maintained higher levels of ABA for longer than do larvae reared at 348C, suggesting a biological significance for ABA. Larvae fed with an ABA-supplemented diet completely prevent the low survival rate due to cold stress and accelerate adult emergence. ABA modulates the expression of genes involved in metabolic adjustments and stress responses: Hexamerin 70b, Insulin Receptor Substrate, Vitellogenin, and Heat Shock Proteins 70. AmLANCL2, the honeybee ABA receptor, is also regulated by cold stress and ABA. These results support a role for ABA increasing the tolerance of honeybee larvae to low temperatures through priming effects.
publishDate 2017
dc.date.none.fl_str_mv 2017-04-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/66704
Ramírez, Leonor; Negri, Pedro; Sturla, Laura; Guida, Lucrezia; Vigliarolo, Tiziana; et al.; Abscisic acid enhances cold tolerance in honeybee larvae; The Royal Society; Proceedings of the Royal Society of London. Series B: Biological Sciences; 284; 1852; 5-4-2017; 1-9
0962-8452
CONICET Digital
CONICET
url http://hdl.handle.net/11336/66704
identifier_str_mv Ramírez, Leonor; Negri, Pedro; Sturla, Laura; Guida, Lucrezia; Vigliarolo, Tiziana; et al.; Abscisic acid enhances cold tolerance in honeybee larvae; The Royal Society; Proceedings of the Royal Society of London. Series B: Biological Sciences; 284; 1852; 5-4-2017; 1-9
0962-8452
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1098/rspb.2016.2140
info:eu-repo/semantics/altIdentifier/url/https://royalsocietypublishing.org/doi/10.1098/rspb.2016.2140
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5394655/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv The Royal Society
publisher.none.fl_str_mv The Royal Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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