Association mapping unravels the genetics controlling seedling drought stress tolerance in winter wheat

Autores
Schierenbeck, Matías; Alqudah, Ahmad M.; Thabet, Samar G.; Lohwasser, Ulrike; Simón, María Rosa; Börner, Andreas
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Drought is a major constraint in wheat (Triticum aestivum L.) grain yield. The present work aimed to identify quantitative trait nucleotides (QTNs)/ candidate genes influencing drought tolerance-related traits at the seedling stage in 261 accessions of a diverse winter wheat panel. Seeds from three consecutive years were exposed to polyethylene glycol 12% (PEG-6000) and a control treatment (distilled water). The Farm-CPU method was used for the association analysis with 17,093 polymorphic SNPs. PEG treatment reduced shoot length (SL) (-36.3%) and root length (RL) (-11.3%) compared with control treatments, while the coleoptile length (CL) was increased by 11% under drought conditions, suggesting that it might be considered as an indicator of stress-tolerance. Interestingly, we revealed 70 stable QTN across 17 chromosomes. Eight QTNs related to more than one trait were detected on chromosomes 1B, 2A (2), 2B, 2D, 4B, 7A, and 7B and located nearby or inside candidate genes within the linkage disequilibrium (LD) interval. For instance, the QTN on chromosome 2D is located inside the gene TraesCS2D02G133900 that controls the variation of CL_S and SL_C. The allelic variation at the candidate genes showed significant influence on the associated traits, demonstrating their role in controlling the natural variation of multi-traits of drought stress tolerance. The gene expression of these candidate genes under different stress conditions validates their biological role in stress tolerance. Our findings offer insight into understanding the genetic factors and diverse mechanisms in response to water shortage conditions that are important for wheat improvement and adaptation at early developmental stages.
Fil: Schierenbeck, Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Leibniz Institute of Plant Genetics and Crop Plant Research; Alemania. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Cátedra de Cerealicultura; Argentina
Fil: Alqudah, Ahmad M.. Qatar University; Qatar
Fil: Thabet, Samar G.. Fayoum University; Egipto
Fil: Lohwasser, Ulrike. Leibniz Institute of Plant Genetics and Crop Plant Research; Alemania
Fil: Simón, María Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Cátedra de Cerealicultura; Argentina
Fil: Börner, Andreas. Leibniz Institute of Plant Genetics and Crop Plant Research; Alemania
Materia
BREEDING
CANDIDATE GENES
DROUGHT TOLERANCE
FARMCPU
GWAS
PEG
SEEDLING
WINTER WHEAT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/222302
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/222302
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Association mapping unravels the genetics controlling seedling drought stress tolerance in winter wheatSchierenbeck, MatíasAlqudah, Ahmad M.Thabet, Samar G.Lohwasser, UlrikeSimón, María RosaBörner, AndreasBREEDINGCANDIDATE GENESDROUGHT TOLERANCEFARMCPUGWASPEGSEEDLINGWINTER WHEAThttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Drought is a major constraint in wheat (Triticum aestivum L.) grain yield. The present work aimed to identify quantitative trait nucleotides (QTNs)/ candidate genes influencing drought tolerance-related traits at the seedling stage in 261 accessions of a diverse winter wheat panel. Seeds from three consecutive years were exposed to polyethylene glycol 12% (PEG-6000) and a control treatment (distilled water). The Farm-CPU method was used for the association analysis with 17,093 polymorphic SNPs. PEG treatment reduced shoot length (SL) (-36.3%) and root length (RL) (-11.3%) compared with control treatments, while the coleoptile length (CL) was increased by 11% under drought conditions, suggesting that it might be considered as an indicator of stress-tolerance. Interestingly, we revealed 70 stable QTN across 17 chromosomes. Eight QTNs related to more than one trait were detected on chromosomes 1B, 2A (2), 2B, 2D, 4B, 7A, and 7B and located nearby or inside candidate genes within the linkage disequilibrium (LD) interval. For instance, the QTN on chromosome 2D is located inside the gene TraesCS2D02G133900 that controls the variation of CL_S and SL_C. The allelic variation at the candidate genes showed significant influence on the associated traits, demonstrating their role in controlling the natural variation of multi-traits of drought stress tolerance. The gene expression of these candidate genes under different stress conditions validates their biological role in stress tolerance. Our findings offer insight into understanding the genetic factors and diverse mechanisms in response to water shortage conditions that are important for wheat improvement and adaptation at early developmental stages.Fil: Schierenbeck, Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Leibniz Institute of Plant Genetics and Crop Plant Research; Alemania. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Cátedra de Cerealicultura; ArgentinaFil: Alqudah, Ahmad M.. Qatar University; QatarFil: Thabet, Samar G.. Fayoum University; EgiptoFil: Lohwasser, Ulrike. Leibniz Institute of Plant Genetics and Crop Plant Research; AlemaniaFil: Simón, María Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Cátedra de Cerealicultura; ArgentinaFil: Börner, Andreas. Leibniz Institute of Plant Genetics and Crop Plant Research; AlemaniaFrontiers Media2023-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/222302Schierenbeck, Matías; Alqudah, Ahmad M.; Thabet, Samar G.; Lohwasser, Ulrike; Simón, María Rosa; et al.; Association mapping unravels the genetics controlling seedling drought stress tolerance in winter wheat; Frontiers Media; Frontiers in Plant Science; 14; 2-2023; 1-131664-462XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fpls.2023.1061845/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2023.1061845info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:59Zoai:ri.conicet.gov.ar:11336/222302instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:59.94CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Association mapping unravels the genetics controlling seedling drought stress tolerance in winter wheat
title Association mapping unravels the genetics controlling seedling drought stress tolerance in winter wheat
spellingShingle Association mapping unravels the genetics controlling seedling drought stress tolerance in winter wheat
Schierenbeck, Matías
BREEDING
CANDIDATE GENES
DROUGHT TOLERANCE
FARMCPU
GWAS
PEG
SEEDLING
WINTER WHEAT
title_short Association mapping unravels the genetics controlling seedling drought stress tolerance in winter wheat
title_full Association mapping unravels the genetics controlling seedling drought stress tolerance in winter wheat
title_fullStr Association mapping unravels the genetics controlling seedling drought stress tolerance in winter wheat
title_full_unstemmed Association mapping unravels the genetics controlling seedling drought stress tolerance in winter wheat
title_sort Association mapping unravels the genetics controlling seedling drought stress tolerance in winter wheat
dc.creator.none.fl_str_mv Schierenbeck, Matías
Alqudah, Ahmad M.
Thabet, Samar G.
Lohwasser, Ulrike
Simón, María Rosa
Börner, Andreas
author Schierenbeck, Matías
author_facet Schierenbeck, Matías
Alqudah, Ahmad M.
Thabet, Samar G.
Lohwasser, Ulrike
Simón, María Rosa
Börner, Andreas
author_role author
author2 Alqudah, Ahmad M.
Thabet, Samar G.
Lohwasser, Ulrike
Simón, María Rosa
Börner, Andreas
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BREEDING
CANDIDATE GENES
DROUGHT TOLERANCE
FARMCPU
GWAS
PEG
SEEDLING
WINTER WHEAT
topic BREEDING
CANDIDATE GENES
DROUGHT TOLERANCE
FARMCPU
GWAS
PEG
SEEDLING
WINTER WHEAT
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Drought is a major constraint in wheat (Triticum aestivum L.) grain yield. The present work aimed to identify quantitative trait nucleotides (QTNs)/ candidate genes influencing drought tolerance-related traits at the seedling stage in 261 accessions of a diverse winter wheat panel. Seeds from three consecutive years were exposed to polyethylene glycol 12% (PEG-6000) and a control treatment (distilled water). The Farm-CPU method was used for the association analysis with 17,093 polymorphic SNPs. PEG treatment reduced shoot length (SL) (-36.3%) and root length (RL) (-11.3%) compared with control treatments, while the coleoptile length (CL) was increased by 11% under drought conditions, suggesting that it might be considered as an indicator of stress-tolerance. Interestingly, we revealed 70 stable QTN across 17 chromosomes. Eight QTNs related to more than one trait were detected on chromosomes 1B, 2A (2), 2B, 2D, 4B, 7A, and 7B and located nearby or inside candidate genes within the linkage disequilibrium (LD) interval. For instance, the QTN on chromosome 2D is located inside the gene TraesCS2D02G133900 that controls the variation of CL_S and SL_C. The allelic variation at the candidate genes showed significant influence on the associated traits, demonstrating their role in controlling the natural variation of multi-traits of drought stress tolerance. The gene expression of these candidate genes under different stress conditions validates their biological role in stress tolerance. Our findings offer insight into understanding the genetic factors and diverse mechanisms in response to water shortage conditions that are important for wheat improvement and adaptation at early developmental stages.
Fil: Schierenbeck, Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Leibniz Institute of Plant Genetics and Crop Plant Research; Alemania. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Cátedra de Cerealicultura; Argentina
Fil: Alqudah, Ahmad M.. Qatar University; Qatar
Fil: Thabet, Samar G.. Fayoum University; Egipto
Fil: Lohwasser, Ulrike. Leibniz Institute of Plant Genetics and Crop Plant Research; Alemania
Fil: Simón, María Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Cátedra de Cerealicultura; Argentina
Fil: Börner, Andreas. Leibniz Institute of Plant Genetics and Crop Plant Research; Alemania
description Drought is a major constraint in wheat (Triticum aestivum L.) grain yield. The present work aimed to identify quantitative trait nucleotides (QTNs)/ candidate genes influencing drought tolerance-related traits at the seedling stage in 261 accessions of a diverse winter wheat panel. Seeds from three consecutive years were exposed to polyethylene glycol 12% (PEG-6000) and a control treatment (distilled water). The Farm-CPU method was used for the association analysis with 17,093 polymorphic SNPs. PEG treatment reduced shoot length (SL) (-36.3%) and root length (RL) (-11.3%) compared with control treatments, while the coleoptile length (CL) was increased by 11% under drought conditions, suggesting that it might be considered as an indicator of stress-tolerance. Interestingly, we revealed 70 stable QTN across 17 chromosomes. Eight QTNs related to more than one trait were detected on chromosomes 1B, 2A (2), 2B, 2D, 4B, 7A, and 7B and located nearby or inside candidate genes within the linkage disequilibrium (LD) interval. For instance, the QTN on chromosome 2D is located inside the gene TraesCS2D02G133900 that controls the variation of CL_S and SL_C. The allelic variation at the candidate genes showed significant influence on the associated traits, demonstrating their role in controlling the natural variation of multi-traits of drought stress tolerance. The gene expression of these candidate genes under different stress conditions validates their biological role in stress tolerance. Our findings offer insight into understanding the genetic factors and diverse mechanisms in response to water shortage conditions that are important for wheat improvement and adaptation at early developmental stages.
publishDate 2023
dc.date.none.fl_str_mv 2023-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/222302
Schierenbeck, Matías; Alqudah, Ahmad M.; Thabet, Samar G.; Lohwasser, Ulrike; Simón, María Rosa; et al.; Association mapping unravels the genetics controlling seedling drought stress tolerance in winter wheat; Frontiers Media; Frontiers in Plant Science; 14; 2-2023; 1-13
1664-462X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/222302
identifier_str_mv Schierenbeck, Matías; Alqudah, Ahmad M.; Thabet, Samar G.; Lohwasser, Ulrike; Simón, María Rosa; et al.; Association mapping unravels the genetics controlling seedling drought stress tolerance in winter wheat; Frontiers Media; Frontiers in Plant Science; 14; 2-2023; 1-13
1664-462X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fpls.2023.1061845/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2023.1061845
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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