Effectiveness of two sequences of Toxoplasma gondii SAG2 protein to differentiate toxoplasmosis infection stages by measuring IgG, IgA and IgM antibodies

Autores
Costa, Juan Gabriel; Duré, Andrea Belén
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Here, we evaluated two Toxoplasma gondii SAG2 antigen sequences which differ in thepresence of the amino-and carboxy-terminal sequences in one of them. They were testedwith several techniques to differentiate between acute and chronic phase of toxoplasmosis:avidity assay and indirect ELISA for the detection of anti-Toxoplasma IgG, IgA and IgMantibodies. Significant differences were found between them. The best results were obtainedwith the sequence denominated SAG2c, which yielded sensitivity of 73.8% and specificity of 80.3% in the detection of IgG by ELISA. Measurement of IgA yielded 67.2% and 81.8% of sensitivity and specificity, respectively. The avidity assay yielded 100% sensitivity and 81.82% specificity. Finally, the molecular modeling and epitope predictions of the protein are developed to find the antigenic region locations, specific and nonspecific, on three-dimensional structure. This paper is the first report of promising results of SAG2 used for detecting IgA antibodies to differentiate toxoplasmosis infection stages.
Fil: Costa, Juan Gabriel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Duré, Andrea Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Materia
Toxoplasmosis
Sag2
Diagnosis
Infection
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/84688
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/84688
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network_name_str CONICET Digital (CONICET)
spelling Effectiveness of two sequences of Toxoplasma gondii SAG2 protein to differentiate toxoplasmosis infection stages by measuring IgG, IgA and IgM antibodiesCosta, Juan GabrielDuré, Andrea BelénToxoplasmosisSag2DiagnosisInfectionhttps://purl.org/becyt/ford/2.6https://purl.org/becyt/ford/2https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Here, we evaluated two Toxoplasma gondii SAG2 antigen sequences which differ in thepresence of the amino-and carboxy-terminal sequences in one of them. They were testedwith several techniques to differentiate between acute and chronic phase of toxoplasmosis:avidity assay and indirect ELISA for the detection of anti-Toxoplasma IgG, IgA and IgMantibodies. Significant differences were found between them. The best results were obtainedwith the sequence denominated SAG2c, which yielded sensitivity of 73.8% and specificity of 80.3% in the detection of IgG by ELISA. Measurement of IgA yielded 67.2% and 81.8% of sensitivity and specificity, respectively. The avidity assay yielded 100% sensitivity and 81.82% specificity. Finally, the molecular modeling and epitope predictions of the protein are developed to find the antigenic region locations, specific and nonspecific, on three-dimensional structure. This paper is the first report of promising results of SAG2 used for detecting IgA antibodies to differentiate toxoplasmosis infection stages.Fil: Costa, Juan Gabriel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Duré, Andrea Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; ArgentinaMalaysian Soc Parasitology Tropical Medicine2016-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/84688Costa, Juan Gabriel; Duré, Andrea Belén; Effectiveness of two sequences of Toxoplasma gondii SAG2 protein to differentiate toxoplasmosis infection stages by measuring IgG, IgA and IgM antibodies; Malaysian Soc Parasitology Tropical Medicine; Tropical Biomedicine; 33; 2; 3-2016; 246-2590127-5720CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://msptm.org/journal-vol-33-no-2/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:14:51Zoai:ri.conicet.gov.ar:11336/84688instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:14:52.062CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effectiveness of two sequences of Toxoplasma gondii SAG2 protein to differentiate toxoplasmosis infection stages by measuring IgG, IgA and IgM antibodies
title Effectiveness of two sequences of Toxoplasma gondii SAG2 protein to differentiate toxoplasmosis infection stages by measuring IgG, IgA and IgM antibodies
spellingShingle Effectiveness of two sequences of Toxoplasma gondii SAG2 protein to differentiate toxoplasmosis infection stages by measuring IgG, IgA and IgM antibodies
Costa, Juan Gabriel
Toxoplasmosis
Sag2
Diagnosis
Infection
title_short Effectiveness of two sequences of Toxoplasma gondii SAG2 protein to differentiate toxoplasmosis infection stages by measuring IgG, IgA and IgM antibodies
title_full Effectiveness of two sequences of Toxoplasma gondii SAG2 protein to differentiate toxoplasmosis infection stages by measuring IgG, IgA and IgM antibodies
title_fullStr Effectiveness of two sequences of Toxoplasma gondii SAG2 protein to differentiate toxoplasmosis infection stages by measuring IgG, IgA and IgM antibodies
title_full_unstemmed Effectiveness of two sequences of Toxoplasma gondii SAG2 protein to differentiate toxoplasmosis infection stages by measuring IgG, IgA and IgM antibodies
title_sort Effectiveness of two sequences of Toxoplasma gondii SAG2 protein to differentiate toxoplasmosis infection stages by measuring IgG, IgA and IgM antibodies
dc.creator.none.fl_str_mv Costa, Juan Gabriel
Duré, Andrea Belén
author Costa, Juan Gabriel
author_facet Costa, Juan Gabriel
Duré, Andrea Belén
author_role author
author2 Duré, Andrea Belén
author2_role author
dc.subject.none.fl_str_mv Toxoplasmosis
Sag2
Diagnosis
Infection
topic Toxoplasmosis
Sag2
Diagnosis
Infection
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.6
https://purl.org/becyt/ford/2
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Here, we evaluated two Toxoplasma gondii SAG2 antigen sequences which differ in thepresence of the amino-and carboxy-terminal sequences in one of them. They were testedwith several techniques to differentiate between acute and chronic phase of toxoplasmosis:avidity assay and indirect ELISA for the detection of anti-Toxoplasma IgG, IgA and IgMantibodies. Significant differences were found between them. The best results were obtainedwith the sequence denominated SAG2c, which yielded sensitivity of 73.8% and specificity of 80.3% in the detection of IgG by ELISA. Measurement of IgA yielded 67.2% and 81.8% of sensitivity and specificity, respectively. The avidity assay yielded 100% sensitivity and 81.82% specificity. Finally, the molecular modeling and epitope predictions of the protein are developed to find the antigenic region locations, specific and nonspecific, on three-dimensional structure. This paper is the first report of promising results of SAG2 used for detecting IgA antibodies to differentiate toxoplasmosis infection stages.
Fil: Costa, Juan Gabriel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Duré, Andrea Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
description Here, we evaluated two Toxoplasma gondii SAG2 antigen sequences which differ in thepresence of the amino-and carboxy-terminal sequences in one of them. They were testedwith several techniques to differentiate between acute and chronic phase of toxoplasmosis:avidity assay and indirect ELISA for the detection of anti-Toxoplasma IgG, IgA and IgMantibodies. Significant differences were found between them. The best results were obtainedwith the sequence denominated SAG2c, which yielded sensitivity of 73.8% and specificity of 80.3% in the detection of IgG by ELISA. Measurement of IgA yielded 67.2% and 81.8% of sensitivity and specificity, respectively. The avidity assay yielded 100% sensitivity and 81.82% specificity. Finally, the molecular modeling and epitope predictions of the protein are developed to find the antigenic region locations, specific and nonspecific, on three-dimensional structure. This paper is the first report of promising results of SAG2 used for detecting IgA antibodies to differentiate toxoplasmosis infection stages.
publishDate 2016
dc.date.none.fl_str_mv 2016-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/84688
Costa, Juan Gabriel; Duré, Andrea Belén; Effectiveness of two sequences of Toxoplasma gondii SAG2 protein to differentiate toxoplasmosis infection stages by measuring IgG, IgA and IgM antibodies; Malaysian Soc Parasitology Tropical Medicine; Tropical Biomedicine; 33; 2; 3-2016; 246-259
0127-5720
CONICET Digital
CONICET
url http://hdl.handle.net/11336/84688
identifier_str_mv Costa, Juan Gabriel; Duré, Andrea Belén; Effectiveness of two sequences of Toxoplasma gondii SAG2 protein to differentiate toxoplasmosis infection stages by measuring IgG, IgA and IgM antibodies; Malaysian Soc Parasitology Tropical Medicine; Tropical Biomedicine; 33; 2; 3-2016; 246-259
0127-5720
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://msptm.org/journal-vol-33-no-2/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Malaysian Soc Parasitology Tropical Medicine
publisher.none.fl_str_mv Malaysian Soc Parasitology Tropical Medicine
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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