Active calcium mobilization from a thapsigargin-sensitive pool by free 32.5n6 in spermatids

Autores
Santiago Valtierra, Florencia Ximena; Peñalva, Daniel Alejandro; Luquez, Jessica Mariela; Aveldaño, Marta Isabel; Reyes, Juan Guillermo; Oresti, Gerardo Martin
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
In their free form, long chain (C18–22) polyunsaturated fatty acids (PUFA), especially 20:4n−6, can modify calcium homeostasis in male germ cells. These cells also contain unusual n-6 very-long-chain (VLC) PUFA (28:4, 30:5, and 32:5), in non-hydroxy (n-V) and 2-hydroxy (h-V) forms, in their membrane sphingolipids. Potential biological roles of VLCPUFA, as free fatty acids (FFA), in the physiology of male germ cells are unknown. In this study we explored the ability of n-V FFA, and their h-V counterparts, to modify the intracellular calcium homeostasis in rat spermatids. After obtaining the n-V and h-V FFA from testicular sphingomyelin, their natural source, each group was separately added to spermatids suspensions at an 8 µM concentration. The n-V FFA increased the intracellular calcium concentration ([Ca2+]i) in spermatids several fold more intensely than did the h-V FFA. After isolating the components of n-V and h-V mixtures by HPLC to study the effect of each VLCPUFA, free n-32:5 was found to be the most active in increasing the [Ca2+]i, followed by n-30:5, while h-32:5 augmented it only slightly. In addition to fatty acid-specific, the response was dose-dependent. The rates of [Ca2+]i upsurge were independent of the presence of extracellular calcium. Pretreatment with thapsigargin inhibited the effect of n-32:5, suggesting that this FFA promotes the release of Ca2+ from intracellular calcium stores, mainly the endoplasmic reticulum. The n-V FFA did not seem to exert their effects through the G protein-coupled receptor GPR120, a putative receptor for free PUFA, as they occurred in the presence of a GPR120 inhibitor. Ceramides containing the same fatty acids did not modify [Ca2+]i, thus the observed [Ca2+]i increases may be attributed to the FFA themselves. The possibility that they occur after VLC-FFAs are converted into other bioactive compounds remains to be investigated. Our results revealed a biological activity of VLCPUFA that suggests a physiological role for these fatty acids. As VLCPUFA-mediated Ca2+ rises occurred in spermatids, they may activate Ca2+ signaling pathways with specific functional targets in germ cells differentiation.
Fil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Peñalva, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Reyes, Juan Guillermo. Pontificia Universidad Católica de Chile; Chile
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology (SAIB); XV Annual Meeting Argentinean Society for General Microbiology (SAMIGE)
Mendoza
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Sociedad Argentina de Microbiología General
Materia
FREE VLCPUFA
CALCIUM HOMEOSTASIS
GERM CELLS
SPERMATOGENESIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/156881
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/156881
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Active calcium mobilization from a thapsigargin-sensitive pool by free 32.5n6 in spermatidsSantiago Valtierra, Florencia XimenaPeñalva, Daniel AlejandroLuquez, Jessica MarielaAveldaño, Marta IsabelReyes, Juan GuillermoOresti, Gerardo MartinFREE VLCPUFACALCIUM HOMEOSTASISGERM CELLSSPERMATOGENESIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In their free form, long chain (C18–22) polyunsaturated fatty acids (PUFA), especially 20:4n−6, can modify calcium homeostasis in male germ cells. These cells also contain unusual n-6 very-long-chain (VLC) PUFA (28:4, 30:5, and 32:5), in non-hydroxy (n-V) and 2-hydroxy (h-V) forms, in their membrane sphingolipids. Potential biological roles of VLCPUFA, as free fatty acids (FFA), in the physiology of male germ cells are unknown. In this study we explored the ability of n-V FFA, and their h-V counterparts, to modify the intracellular calcium homeostasis in rat spermatids. After obtaining the n-V and h-V FFA from testicular sphingomyelin, their natural source, each group was separately added to spermatids suspensions at an 8 µM concentration. The n-V FFA increased the intracellular calcium concentration ([Ca2+]i) in spermatids several fold more intensely than did the h-V FFA. After isolating the components of n-V and h-V mixtures by HPLC to study the effect of each VLCPUFA, free n-32:5 was found to be the most active in increasing the [Ca2+]i, followed by n-30:5, while h-32:5 augmented it only slightly. In addition to fatty acid-specific, the response was dose-dependent. The rates of [Ca2+]i upsurge were independent of the presence of extracellular calcium. Pretreatment with thapsigargin inhibited the effect of n-32:5, suggesting that this FFA promotes the release of Ca2+ from intracellular calcium stores, mainly the endoplasmic reticulum. The n-V FFA did not seem to exert their effects through the G protein-coupled receptor GPR120, a putative receptor for free PUFA, as they occurred in the presence of a GPR120 inhibitor. Ceramides containing the same fatty acids did not modify [Ca2+]i, thus the observed [Ca2+]i increases may be attributed to the FFA themselves. The possibility that they occur after VLC-FFAs are converted into other bioactive compounds remains to be investigated. Our results revealed a biological activity of VLCPUFA that suggests a physiological role for these fatty acids. As VLCPUFA-mediated Ca2+ rises occurred in spermatids, they may activate Ca2+ signaling pathways with specific functional targets in germ cells differentiation.Fil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Peñalva, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Reyes, Juan Guillermo. Pontificia Universidad Católica de Chile; ChileFil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaLVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology (SAIB); XV Annual Meeting Argentinean Society for General Microbiology (SAMIGE)MendozaArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularSociedad Argentina de Microbiología GeneralTech Science Press2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/156881Active calcium mobilization from a thapsigargin-sensitive pool by free 32.5n6 in spermatids; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology (SAIB); XV Annual Meeting Argentinean Society for General Microbiology (SAMIGE); Mendoza; Argentina; 2020; 1-70327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/index.php?q=node/562Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:53:08Zoai:ri.conicet.gov.ar:11336/156881instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:53:08.38CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Active calcium mobilization from a thapsigargin-sensitive pool by free 32.5n6 in spermatids
title Active calcium mobilization from a thapsigargin-sensitive pool by free 32.5n6 in spermatids
spellingShingle Active calcium mobilization from a thapsigargin-sensitive pool by free 32.5n6 in spermatids
Santiago Valtierra, Florencia Ximena
FREE VLCPUFA
CALCIUM HOMEOSTASIS
GERM CELLS
SPERMATOGENESIS
title_short Active calcium mobilization from a thapsigargin-sensitive pool by free 32.5n6 in spermatids
title_full Active calcium mobilization from a thapsigargin-sensitive pool by free 32.5n6 in spermatids
title_fullStr Active calcium mobilization from a thapsigargin-sensitive pool by free 32.5n6 in spermatids
title_full_unstemmed Active calcium mobilization from a thapsigargin-sensitive pool by free 32.5n6 in spermatids
title_sort Active calcium mobilization from a thapsigargin-sensitive pool by free 32.5n6 in spermatids
dc.creator.none.fl_str_mv Santiago Valtierra, Florencia Ximena
Peñalva, Daniel Alejandro
Luquez, Jessica Mariela
Aveldaño, Marta Isabel
Reyes, Juan Guillermo
Oresti, Gerardo Martin
author Santiago Valtierra, Florencia Ximena
author_facet Santiago Valtierra, Florencia Ximena
Peñalva, Daniel Alejandro
Luquez, Jessica Mariela
Aveldaño, Marta Isabel
Reyes, Juan Guillermo
Oresti, Gerardo Martin
author_role author
author2 Peñalva, Daniel Alejandro
Luquez, Jessica Mariela
Aveldaño, Marta Isabel
Reyes, Juan Guillermo
Oresti, Gerardo Martin
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv FREE VLCPUFA
CALCIUM HOMEOSTASIS
GERM CELLS
SPERMATOGENESIS
topic FREE VLCPUFA
CALCIUM HOMEOSTASIS
GERM CELLS
SPERMATOGENESIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In their free form, long chain (C18–22) polyunsaturated fatty acids (PUFA), especially 20:4n−6, can modify calcium homeostasis in male germ cells. These cells also contain unusual n-6 very-long-chain (VLC) PUFA (28:4, 30:5, and 32:5), in non-hydroxy (n-V) and 2-hydroxy (h-V) forms, in their membrane sphingolipids. Potential biological roles of VLCPUFA, as free fatty acids (FFA), in the physiology of male germ cells are unknown. In this study we explored the ability of n-V FFA, and their h-V counterparts, to modify the intracellular calcium homeostasis in rat spermatids. After obtaining the n-V and h-V FFA from testicular sphingomyelin, their natural source, each group was separately added to spermatids suspensions at an 8 µM concentration. The n-V FFA increased the intracellular calcium concentration ([Ca2+]i) in spermatids several fold more intensely than did the h-V FFA. After isolating the components of n-V and h-V mixtures by HPLC to study the effect of each VLCPUFA, free n-32:5 was found to be the most active in increasing the [Ca2+]i, followed by n-30:5, while h-32:5 augmented it only slightly. In addition to fatty acid-specific, the response was dose-dependent. The rates of [Ca2+]i upsurge were independent of the presence of extracellular calcium. Pretreatment with thapsigargin inhibited the effect of n-32:5, suggesting that this FFA promotes the release of Ca2+ from intracellular calcium stores, mainly the endoplasmic reticulum. The n-V FFA did not seem to exert their effects through the G protein-coupled receptor GPR120, a putative receptor for free PUFA, as they occurred in the presence of a GPR120 inhibitor. Ceramides containing the same fatty acids did not modify [Ca2+]i, thus the observed [Ca2+]i increases may be attributed to the FFA themselves. The possibility that they occur after VLC-FFAs are converted into other bioactive compounds remains to be investigated. Our results revealed a biological activity of VLCPUFA that suggests a physiological role for these fatty acids. As VLCPUFA-mediated Ca2+ rises occurred in spermatids, they may activate Ca2+ signaling pathways with specific functional targets in germ cells differentiation.
Fil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Peñalva, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Reyes, Juan Guillermo. Pontificia Universidad Católica de Chile; Chile
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology (SAIB); XV Annual Meeting Argentinean Society for General Microbiology (SAMIGE)
Mendoza
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Sociedad Argentina de Microbiología General
description In their free form, long chain (C18–22) polyunsaturated fatty acids (PUFA), especially 20:4n−6, can modify calcium homeostasis in male germ cells. These cells also contain unusual n-6 very-long-chain (VLC) PUFA (28:4, 30:5, and 32:5), in non-hydroxy (n-V) and 2-hydroxy (h-V) forms, in their membrane sphingolipids. Potential biological roles of VLCPUFA, as free fatty acids (FFA), in the physiology of male germ cells are unknown. In this study we explored the ability of n-V FFA, and their h-V counterparts, to modify the intracellular calcium homeostasis in rat spermatids. After obtaining the n-V and h-V FFA from testicular sphingomyelin, their natural source, each group was separately added to spermatids suspensions at an 8 µM concentration. The n-V FFA increased the intracellular calcium concentration ([Ca2+]i) in spermatids several fold more intensely than did the h-V FFA. After isolating the components of n-V and h-V mixtures by HPLC to study the effect of each VLCPUFA, free n-32:5 was found to be the most active in increasing the [Ca2+]i, followed by n-30:5, while h-32:5 augmented it only slightly. In addition to fatty acid-specific, the response was dose-dependent. The rates of [Ca2+]i upsurge were independent of the presence of extracellular calcium. Pretreatment with thapsigargin inhibited the effect of n-32:5, suggesting that this FFA promotes the release of Ca2+ from intracellular calcium stores, mainly the endoplasmic reticulum. The n-V FFA did not seem to exert their effects through the G protein-coupled receptor GPR120, a putative receptor for free PUFA, as they occurred in the presence of a GPR120 inhibitor. Ceramides containing the same fatty acids did not modify [Ca2+]i, thus the observed [Ca2+]i increases may be attributed to the FFA themselves. The possibility that they occur after VLC-FFAs are converted into other bioactive compounds remains to be investigated. Our results revealed a biological activity of VLCPUFA that suggests a physiological role for these fatty acids. As VLCPUFA-mediated Ca2+ rises occurred in spermatids, they may activate Ca2+ signaling pathways with specific functional targets in germ cells differentiation.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/156881
Active calcium mobilization from a thapsigargin-sensitive pool by free 32.5n6 in spermatids; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology (SAIB); XV Annual Meeting Argentinean Society for General Microbiology (SAMIGE); Mendoza; Argentina; 2020; 1-7
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/156881
identifier_str_mv Active calcium mobilization from a thapsigargin-sensitive pool by free 32.5n6 in spermatids; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology (SAIB); XV Annual Meeting Argentinean Society for General Microbiology (SAMIGE); Mendoza; Argentina; 2020; 1-7
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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eu_rights_str_mv openAccess
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application/pdf
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dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
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