Expression of elovl4 and fa2h with spermatogenic cell differentiation In the rat testis

Autores
Santiago Valtierra, Florencia Ximena; Peñalva, Daniel Alejandro; Luquez, Jessica Mariela; Furland, Natalia Edith; Aveldaño, Marta Isabel; Oresti, Gerardo Martin
Año de publicación
2016
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Rat spermatogenic cell membranes contain sphingolipids with nonhydroxy and 2-hydroxy very long chain (C24-32) PUFA. The biosynthesis of such fatty acids requires the expression of very long chain fatty acid elongases (Elovl4 for > C24) and a fatty acid 2- hydroxylase (Fa2h). In this study, mRNA levels of Elovl4and Fa2h were measured by qPCR in rat testis at different postnatal ages and in cells isolated from the seminiferous epithelium of adults. At early prepuberal ages (P14), Elovl4 was highly expressed while Fa2h mRNA was absent. Fa2h started to be detected at P25-30 and increased thereafter, while Elovl4mRNA levels decreased. The expression of both genes, but mainly Fa2h, was markedly reduced in adult testes that had been depleted of germ cells by mild hyperthermia. In isolated spermatogenic cells, both genes were expressed at lower levels in pachytene spermatocytes than in postmeiotic round and late spermatids. Interestingly, Sertoli cells had high Elovl4but lacked Fa2h mRNA. The Elovl4 protein was detected in spermatocytes from P21 to adulthood, when the protein was clearly observed in elongated spermatids. The Elovl4 enzyme was functional in germ cells, as these cells, in culture, were able to elongate [3H]20:4 to PUFA longer than C24. Our results underscore the presence of a well-timed, cell-specific regulation of Elovl4 and Fa2h in germ cells as differentiation proceeds.
Fil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Peñalva, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
52 th Annual Meeting Argentine Society for Biochemistry and Molecular Biology
Córdoba
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
SPHINGOLIPIDS
VLCPUFA
ELOVL4
FA2H
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/234271
Acceder
id CONICETDig_c83d83bcd705c94334a095e7d1d5e011
oai_identifier_str oai:ri.conicet.gov.ar:11336/234271
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expression of elovl4 and fa2h with spermatogenic cell differentiation In the rat testisSantiago Valtierra, Florencia XimenaPeñalva, Daniel AlejandroLuquez, Jessica MarielaFurland, Natalia EdithAveldaño, Marta IsabelOresti, Gerardo MartinSPHINGOLIPIDSVLCPUFAELOVL4FA2Hhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Rat spermatogenic cell membranes contain sphingolipids with nonhydroxy and 2-hydroxy very long chain (C24-32) PUFA. The biosynthesis of such fatty acids requires the expression of very long chain fatty acid elongases (Elovl4 for > C24) and a fatty acid 2- hydroxylase (Fa2h). In this study, mRNA levels of Elovl4and Fa2h were measured by qPCR in rat testis at different postnatal ages and in cells isolated from the seminiferous epithelium of adults. At early prepuberal ages (P14), Elovl4 was highly expressed while Fa2h mRNA was absent. Fa2h started to be detected at P25-30 and increased thereafter, while Elovl4mRNA levels decreased. The expression of both genes, but mainly Fa2h, was markedly reduced in adult testes that had been depleted of germ cells by mild hyperthermia. In isolated spermatogenic cells, both genes were expressed at lower levels in pachytene spermatocytes than in postmeiotic round and late spermatids. Interestingly, Sertoli cells had high Elovl4but lacked Fa2h mRNA. The Elovl4 protein was detected in spermatocytes from P21 to adulthood, when the protein was clearly observed in elongated spermatids. The Elovl4 enzyme was functional in germ cells, as these cells, in culture, were able to elongate [3H]20:4 to PUFA longer than C24. Our results underscore the presence of a well-timed, cell-specific regulation of Elovl4 and Fa2h in germ cells as differentiation proceeds.Fil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Peñalva, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina52 th Annual Meeting Argentine Society for Biochemistry and Molecular BiologyCórdobaArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularTech Science Press2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/234271Expression of elovl4 and fa2h with spermatogenic cell differentiation In the rat testis; 52 th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; Córdoba; Argentina; 2016; 37-370327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v40nSuppl.S/34023/pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:18:33Zoai:ri.conicet.gov.ar:11336/234271instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:18:33.248CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expression of elovl4 and fa2h with spermatogenic cell differentiation In the rat testis
title Expression of elovl4 and fa2h with spermatogenic cell differentiation In the rat testis
spellingShingle Expression of elovl4 and fa2h with spermatogenic cell differentiation In the rat testis
Santiago Valtierra, Florencia Ximena
SPHINGOLIPIDS
VLCPUFA
ELOVL4
FA2H
title_short Expression of elovl4 and fa2h with spermatogenic cell differentiation In the rat testis
title_full Expression of elovl4 and fa2h with spermatogenic cell differentiation In the rat testis
title_fullStr Expression of elovl4 and fa2h with spermatogenic cell differentiation In the rat testis
title_full_unstemmed Expression of elovl4 and fa2h with spermatogenic cell differentiation In the rat testis
title_sort Expression of elovl4 and fa2h with spermatogenic cell differentiation In the rat testis
dc.creator.none.fl_str_mv Santiago Valtierra, Florencia Ximena
Peñalva, Daniel Alejandro
Luquez, Jessica Mariela
Furland, Natalia Edith
Aveldaño, Marta Isabel
Oresti, Gerardo Martin
author Santiago Valtierra, Florencia Ximena
author_facet Santiago Valtierra, Florencia Ximena
Peñalva, Daniel Alejandro
Luquez, Jessica Mariela
Furland, Natalia Edith
Aveldaño, Marta Isabel
Oresti, Gerardo Martin
author_role author
author2 Peñalva, Daniel Alejandro
Luquez, Jessica Mariela
Furland, Natalia Edith
Aveldaño, Marta Isabel
Oresti, Gerardo Martin
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv SPHINGOLIPIDS
VLCPUFA
ELOVL4
FA2H
topic SPHINGOLIPIDS
VLCPUFA
ELOVL4
FA2H
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Rat spermatogenic cell membranes contain sphingolipids with nonhydroxy and 2-hydroxy very long chain (C24-32) PUFA. The biosynthesis of such fatty acids requires the expression of very long chain fatty acid elongases (Elovl4 for > C24) and a fatty acid 2- hydroxylase (Fa2h). In this study, mRNA levels of Elovl4and Fa2h were measured by qPCR in rat testis at different postnatal ages and in cells isolated from the seminiferous epithelium of adults. At early prepuberal ages (P14), Elovl4 was highly expressed while Fa2h mRNA was absent. Fa2h started to be detected at P25-30 and increased thereafter, while Elovl4mRNA levels decreased. The expression of both genes, but mainly Fa2h, was markedly reduced in adult testes that had been depleted of germ cells by mild hyperthermia. In isolated spermatogenic cells, both genes were expressed at lower levels in pachytene spermatocytes than in postmeiotic round and late spermatids. Interestingly, Sertoli cells had high Elovl4but lacked Fa2h mRNA. The Elovl4 protein was detected in spermatocytes from P21 to adulthood, when the protein was clearly observed in elongated spermatids. The Elovl4 enzyme was functional in germ cells, as these cells, in culture, were able to elongate [3H]20:4 to PUFA longer than C24. Our results underscore the presence of a well-timed, cell-specific regulation of Elovl4 and Fa2h in germ cells as differentiation proceeds.
Fil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Peñalva, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
52 th Annual Meeting Argentine Society for Biochemistry and Molecular Biology
Córdoba
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description Rat spermatogenic cell membranes contain sphingolipids with nonhydroxy and 2-hydroxy very long chain (C24-32) PUFA. The biosynthesis of such fatty acids requires the expression of very long chain fatty acid elongases (Elovl4 for > C24) and a fatty acid 2- hydroxylase (Fa2h). In this study, mRNA levels of Elovl4and Fa2h were measured by qPCR in rat testis at different postnatal ages and in cells isolated from the seminiferous epithelium of adults. At early prepuberal ages (P14), Elovl4 was highly expressed while Fa2h mRNA was absent. Fa2h started to be detected at P25-30 and increased thereafter, while Elovl4mRNA levels decreased. The expression of both genes, but mainly Fa2h, was markedly reduced in adult testes that had been depleted of germ cells by mild hyperthermia. In isolated spermatogenic cells, both genes were expressed at lower levels in pachytene spermatocytes than in postmeiotic round and late spermatids. Interestingly, Sertoli cells had high Elovl4but lacked Fa2h mRNA. The Elovl4 protein was detected in spermatocytes from P21 to adulthood, when the protein was clearly observed in elongated spermatids. The Elovl4 enzyme was functional in germ cells, as these cells, in culture, were able to elongate [3H]20:4 to PUFA longer than C24. Our results underscore the presence of a well-timed, cell-specific regulation of Elovl4 and Fa2h in germ cells as differentiation proceeds.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/234271
Expression of elovl4 and fa2h with spermatogenic cell differentiation In the rat testis; 52 th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; Córdoba; Argentina; 2016; 37-37
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/234271
identifier_str_mv Expression of elovl4 and fa2h with spermatogenic cell differentiation In the rat testis; 52 th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; Córdoba; Argentina; 2016; 37-37
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v40nSuppl.S/34023/pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614148657250304
score 13.070432

Publicaciones similares