Sertoli–immature spermatids disengagement during testis regression in the armadillo

Autores
Merico, Valeria; Luaces, Juan Pablo; Rossi, Luis Francisco; Rebuzzini, Paola; Merani, Maria Susana; Zuccotti, Maurizio; Garagna, Silvia
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In nature, mammalian seasonal breeders undergo spermatogenetic arrest during the non-breeding season. In the large hairy armadillo Chaetophractus villosus, testis regression initiates with immature post-meiotic germ cells sloughing into the tubule lumen and continues with the death of the remaining spermatocytes. At the end of the regression period, only spermatogonia and Sertoli cells persist in the seminiferous epithelium. It has been suggested that cell sloughing is determined by changes in the adhesion complexes between Sertoli cells and spermatids, which are mediated by low intra-testicular testosterone levels. By immunofluorescence and Western blotting we studied key proteins of the N-cadherin/N-cadherin and A6B1-integrin/laminin interlocks that contribute to the complex Sertoli/spermatid adhesion system throughout the eight stages of the seminiferous epithelium cycle in the comparison between active and regressing testes. In active testis, B1-integrin, laminin G3, N-cadherin, B-catenin, P-B-catenin-Tyr654, FAK, P-FAK-Tyr397, SRC, P-SRC-Tyr416 proteins present a spermatogenetic cycle-dependent localisation pattern, unmaintained in regressing testes. In the latter, quantitative variations and changes in the phosphorylation state of protein FAK, SRC and B-catenin contribute to the disassembly of the N-cadherin/N-cadherin and A6B1-integrin/laminin interlocks, thus promoting the massive release of immature spermatids.
Fil: Merico, Valeria. Universita degli Studi di Pavia; Italia
Fil: Luaces, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Rossi, Luis Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Rebuzzini, Paola. Universita degli Studi di Pavia; Italia
Fil: Merani, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Zuccotti, Maurizio. Universita degli Studi di Pavia; Italia
Fil: Garagna, Silvia. Universita degli Studi di Pavia; Italia
Materia
Seasonal reproduction
armadillo
spermatogenesis
testis regression
adherens junctions
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/160168
Acceder
id CONICETDig_45963ffda945900e7d8665f9a9586215
oai_identifier_str oai:ri.conicet.gov.ar:11336/160168
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sertoli–immature spermatids disengagement during testis regression in the armadilloMerico, ValeriaLuaces, Juan PabloRossi, Luis FranciscoRebuzzini, PaolaMerani, Maria SusanaZuccotti, MaurizioGaragna, SilviaSeasonal reproductionarmadillospermatogenesistestis regressionadherens junctionshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In nature, mammalian seasonal breeders undergo spermatogenetic arrest during the non-breeding season. In the large hairy armadillo Chaetophractus villosus, testis regression initiates with immature post-meiotic germ cells sloughing into the tubule lumen and continues with the death of the remaining spermatocytes. At the end of the regression period, only spermatogonia and Sertoli cells persist in the seminiferous epithelium. It has been suggested that cell sloughing is determined by changes in the adhesion complexes between Sertoli cells and spermatids, which are mediated by low intra-testicular testosterone levels. By immunofluorescence and Western blotting we studied key proteins of the N-cadherin/N-cadherin and A6B1-integrin/laminin interlocks that contribute to the complex Sertoli/spermatid adhesion system throughout the eight stages of the seminiferous epithelium cycle in the comparison between active and regressing testes. In active testis, B1-integrin, laminin G3, N-cadherin, B-catenin, P-B-catenin-Tyr654, FAK, P-FAK-Tyr397, SRC, P-SRC-Tyr416 proteins present a spermatogenetic cycle-dependent localisation pattern, unmaintained in regressing testes. In the latter, quantitative variations and changes in the phosphorylation state of protein FAK, SRC and B-catenin contribute to the disassembly of the N-cadherin/N-cadherin and A6B1-integrin/laminin interlocks, thus promoting the massive release of immature spermatids.Fil: Merico, Valeria. Universita degli Studi di Pavia; ItaliaFil: Luaces, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; ArgentinaFil: Rossi, Luis Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Rebuzzini, Paola. Universita degli Studi di Pavia; ItaliaFil: Merani, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Zuccotti, Maurizio. Universita degli Studi di Pavia; ItaliaFil: Garagna, Silvia. Universita degli Studi di Pavia; ItaliaBioScientifica2019-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/160168Merico, Valeria; Luaces, Juan Pablo; Rossi, Luis Francisco; Rebuzzini, Paola; Merani, Maria Susana; et al.; Sertoli–immature spermatids disengagement during testis regression in the armadillo; BioScientifica; Reproduction; 157; 1; 1-2019; 27-421470-1626CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1530/REP-18-0006info:eu-repo/semantics/altIdentifier/url/https://rep.bioscientifica.com/configurable/content/journals$002frep$002f157$002f1$002fREP-18-0006.xml?t:ac=journals%24002frep%24002f157%24002f1%24002fREP-18-0006.xmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:11:56Zoai:ri.conicet.gov.ar:11336/160168instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:11:56.499CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sertoli–immature spermatids disengagement during testis regression in the armadillo
title Sertoli–immature spermatids disengagement during testis regression in the armadillo
spellingShingle Sertoli–immature spermatids disengagement during testis regression in the armadillo
Merico, Valeria
Seasonal reproduction
armadillo
spermatogenesis
testis regression
adherens junctions
title_short Sertoli–immature spermatids disengagement during testis regression in the armadillo
title_full Sertoli–immature spermatids disengagement during testis regression in the armadillo
title_fullStr Sertoli–immature spermatids disengagement during testis regression in the armadillo
title_full_unstemmed Sertoli–immature spermatids disengagement during testis regression in the armadillo
title_sort Sertoli–immature spermatids disengagement during testis regression in the armadillo
dc.creator.none.fl_str_mv Merico, Valeria
Luaces, Juan Pablo
Rossi, Luis Francisco
Rebuzzini, Paola
Merani, Maria Susana
Zuccotti, Maurizio
Garagna, Silvia
author Merico, Valeria
author_facet Merico, Valeria
Luaces, Juan Pablo
Rossi, Luis Francisco
Rebuzzini, Paola
Merani, Maria Susana
Zuccotti, Maurizio
Garagna, Silvia
author_role author
author2 Luaces, Juan Pablo
Rossi, Luis Francisco
Rebuzzini, Paola
Merani, Maria Susana
Zuccotti, Maurizio
Garagna, Silvia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Seasonal reproduction
armadillo
spermatogenesis
testis regression
adherens junctions
topic Seasonal reproduction
armadillo
spermatogenesis
testis regression
adherens junctions
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In nature, mammalian seasonal breeders undergo spermatogenetic arrest during the non-breeding season. In the large hairy armadillo Chaetophractus villosus, testis regression initiates with immature post-meiotic germ cells sloughing into the tubule lumen and continues with the death of the remaining spermatocytes. At the end of the regression period, only spermatogonia and Sertoli cells persist in the seminiferous epithelium. It has been suggested that cell sloughing is determined by changes in the adhesion complexes between Sertoli cells and spermatids, which are mediated by low intra-testicular testosterone levels. By immunofluorescence and Western blotting we studied key proteins of the N-cadherin/N-cadherin and A6B1-integrin/laminin interlocks that contribute to the complex Sertoli/spermatid adhesion system throughout the eight stages of the seminiferous epithelium cycle in the comparison between active and regressing testes. In active testis, B1-integrin, laminin G3, N-cadherin, B-catenin, P-B-catenin-Tyr654, FAK, P-FAK-Tyr397, SRC, P-SRC-Tyr416 proteins present a spermatogenetic cycle-dependent localisation pattern, unmaintained in regressing testes. In the latter, quantitative variations and changes in the phosphorylation state of protein FAK, SRC and B-catenin contribute to the disassembly of the N-cadherin/N-cadherin and A6B1-integrin/laminin interlocks, thus promoting the massive release of immature spermatids.
Fil: Merico, Valeria. Universita degli Studi di Pavia; Italia
Fil: Luaces, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Rossi, Luis Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Rebuzzini, Paola. Universita degli Studi di Pavia; Italia
Fil: Merani, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Zuccotti, Maurizio. Universita degli Studi di Pavia; Italia
Fil: Garagna, Silvia. Universita degli Studi di Pavia; Italia
description In nature, mammalian seasonal breeders undergo spermatogenetic arrest during the non-breeding season. In the large hairy armadillo Chaetophractus villosus, testis regression initiates with immature post-meiotic germ cells sloughing into the tubule lumen and continues with the death of the remaining spermatocytes. At the end of the regression period, only spermatogonia and Sertoli cells persist in the seminiferous epithelium. It has been suggested that cell sloughing is determined by changes in the adhesion complexes between Sertoli cells and spermatids, which are mediated by low intra-testicular testosterone levels. By immunofluorescence and Western blotting we studied key proteins of the N-cadherin/N-cadherin and A6B1-integrin/laminin interlocks that contribute to the complex Sertoli/spermatid adhesion system throughout the eight stages of the seminiferous epithelium cycle in the comparison between active and regressing testes. In active testis, B1-integrin, laminin G3, N-cadherin, B-catenin, P-B-catenin-Tyr654, FAK, P-FAK-Tyr397, SRC, P-SRC-Tyr416 proteins present a spermatogenetic cycle-dependent localisation pattern, unmaintained in regressing testes. In the latter, quantitative variations and changes in the phosphorylation state of protein FAK, SRC and B-catenin contribute to the disassembly of the N-cadherin/N-cadherin and A6B1-integrin/laminin interlocks, thus promoting the massive release of immature spermatids.
publishDate 2019
dc.date.none.fl_str_mv 2019-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/160168
Merico, Valeria; Luaces, Juan Pablo; Rossi, Luis Francisco; Rebuzzini, Paola; Merani, Maria Susana; et al.; Sertoli–immature spermatids disengagement during testis regression in the armadillo; BioScientifica; Reproduction; 157; 1; 1-2019; 27-42
1470-1626
CONICET Digital
CONICET
url http://hdl.handle.net/11336/160168
identifier_str_mv Merico, Valeria; Luaces, Juan Pablo; Rossi, Luis Francisco; Rebuzzini, Paola; Merani, Maria Susana; et al.; Sertoli–immature spermatids disengagement during testis regression in the armadillo; BioScientifica; Reproduction; 157; 1; 1-2019; 27-42
1470-1626
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1530/REP-18-0006
info:eu-repo/semantics/altIdentifier/url/https://rep.bioscientifica.com/configurable/content/journals$002frep$002f157$002f1$002fREP-18-0006.xml?t:ac=journals%24002frep%24002f157%24002f1%24002fREP-18-0006.xml
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioScientifica
publisher.none.fl_str_mv BioScientifica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1847977654783836160
score 13.082534

Publicaciones similares