Biosynthesis of sphingolipids with very-long-chain pufa a hallmark of differentiating male germ cells

Autores
Oresti, Gerardo Martin; Santiago Valtierra, Florencia Ximena; Aveldaño, Marta Isabel
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The sphingomyelins (SM) and ceramides (Cer) of rodent spermatogenic cells contain very-long-chain (C28-C32) polyenoic fatty acids (VLCPUFA), in non-hydroxy (n-V) and 2-hydroxy (h-V) forms. The SM and Cer species with n-V, present in meiotic spermatocytes, become in part h-V species in post-meiotic spermatids. In each of these cells, the mentioned species are located in the non-raft fraction of the plasma membrane. The enzymes required for PUFA elongation to n-V, Elovl5, Elovl2 and Elovl4, and the fatty acid 2-hydroxylase (Fa2h) that converts n-V to h-V, are expressed in germ cells, with Elovl4 and Fa2h protein levels being highest in spermatocytes and spermatids, respectively. The Cer and SM species with n-V and h-V are biosynthesized de novo in a germ cell type-specific and steroid hormone-dependent manner. CerS3, which specifically N-acylates VLCPUFA to sphinganine, is highly expressed in meiotic cells. The Elovl4 and CerS3 protein expression prevails in spermatocytes, is seminiferous stage-specific, and is mostly concomitant. In spermatids, the Fa2h protein appears concentrated in late stages, especially when they elongate and their heads change shape. The unique sphingolipid species with n-V and h-V, as well as the enzymes involved in their biosynthesis, are useful biomarkers for investigating normal and pathological aspects of germ and sperm cell functions. Supported by SGCyT UNS-PGI-UNS (24/B272 to GMO and 24/B218 to MIA), FONCyT (PICT2017-2535 to GMO).
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
The LV Annual SAIB Meeting and XIV PABMB Congress
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Panamerican Association of Biochemestry and Molecular Biology
Materia
VLCPUFA
SPHINGOLIPIDS
SPERM
GERM CELLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/156614
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/156614
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biosynthesis of sphingolipids with very-long-chain pufa a hallmark of differentiating male germ cellsOresti, Gerardo MartinSantiago Valtierra, Florencia XimenaAveldaño, Marta IsabelVLCPUFASPHINGOLIPIDSSPERMGERM CELLShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The sphingomyelins (SM) and ceramides (Cer) of rodent spermatogenic cells contain very-long-chain (C28-C32) polyenoic fatty acids (VLCPUFA), in non-hydroxy (n-V) and 2-hydroxy (h-V) forms. The SM and Cer species with n-V, present in meiotic spermatocytes, become in part h-V species in post-meiotic spermatids. In each of these cells, the mentioned species are located in the non-raft fraction of the plasma membrane. The enzymes required for PUFA elongation to n-V, Elovl5, Elovl2 and Elovl4, and the fatty acid 2-hydroxylase (Fa2h) that converts n-V to h-V, are expressed in germ cells, with Elovl4 and Fa2h protein levels being highest in spermatocytes and spermatids, respectively. The Cer and SM species with n-V and h-V are biosynthesized de novo in a germ cell type-specific and steroid hormone-dependent manner. CerS3, which specifically N-acylates VLCPUFA to sphinganine, is highly expressed in meiotic cells. The Elovl4 and CerS3 protein expression prevails in spermatocytes, is seminiferous stage-specific, and is mostly concomitant. In spermatids, the Fa2h protein appears concentrated in late stages, especially when they elongate and their heads change shape. The unique sphingolipid species with n-V and h-V, as well as the enzymes involved in their biosynthesis, are useful biomarkers for investigating normal and pathological aspects of germ and sperm cell functions. Supported by SGCyT UNS-PGI-UNS (24/B272 to GMO and 24/B218 to MIA), FONCyT (PICT2017-2535 to GMO).Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaThe LV Annual SAIB Meeting and XIV PABMB CongressArgentinaSociedad Argentina de Investigación Bioquímica y Biología MolecularPanamerican Association of Biochemestry and Molecular BiologyTech Science Press2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/156614Biosynthesis of sphingolipids with very-long-chain pufa a hallmark of differentiating male germ cells; The LV Annual SAIB Meeting and XIV PABMB Congress; Argentina; 2019; 34-341667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/info:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2019-version-final.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:36:58Zoai:ri.conicet.gov.ar:11336/156614instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:36:58.49CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biosynthesis of sphingolipids with very-long-chain pufa a hallmark of differentiating male germ cells
title Biosynthesis of sphingolipids with very-long-chain pufa a hallmark of differentiating male germ cells
spellingShingle Biosynthesis of sphingolipids with very-long-chain pufa a hallmark of differentiating male germ cells
Oresti, Gerardo Martin
VLCPUFA
SPHINGOLIPIDS
SPERM
GERM CELLS
title_short Biosynthesis of sphingolipids with very-long-chain pufa a hallmark of differentiating male germ cells
title_full Biosynthesis of sphingolipids with very-long-chain pufa a hallmark of differentiating male germ cells
title_fullStr Biosynthesis of sphingolipids with very-long-chain pufa a hallmark of differentiating male germ cells
title_full_unstemmed Biosynthesis of sphingolipids with very-long-chain pufa a hallmark of differentiating male germ cells
title_sort Biosynthesis of sphingolipids with very-long-chain pufa a hallmark of differentiating male germ cells
dc.creator.none.fl_str_mv Oresti, Gerardo Martin
Santiago Valtierra, Florencia Ximena
Aveldaño, Marta Isabel
author Oresti, Gerardo Martin
author_facet Oresti, Gerardo Martin
Santiago Valtierra, Florencia Ximena
Aveldaño, Marta Isabel
author_role author
author2 Santiago Valtierra, Florencia Ximena
Aveldaño, Marta Isabel
author2_role author
author
dc.subject.none.fl_str_mv VLCPUFA
SPHINGOLIPIDS
SPERM
GERM CELLS
topic VLCPUFA
SPHINGOLIPIDS
SPERM
GERM CELLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The sphingomyelins (SM) and ceramides (Cer) of rodent spermatogenic cells contain very-long-chain (C28-C32) polyenoic fatty acids (VLCPUFA), in non-hydroxy (n-V) and 2-hydroxy (h-V) forms. The SM and Cer species with n-V, present in meiotic spermatocytes, become in part h-V species in post-meiotic spermatids. In each of these cells, the mentioned species are located in the non-raft fraction of the plasma membrane. The enzymes required for PUFA elongation to n-V, Elovl5, Elovl2 and Elovl4, and the fatty acid 2-hydroxylase (Fa2h) that converts n-V to h-V, are expressed in germ cells, with Elovl4 and Fa2h protein levels being highest in spermatocytes and spermatids, respectively. The Cer and SM species with n-V and h-V are biosynthesized de novo in a germ cell type-specific and steroid hormone-dependent manner. CerS3, which specifically N-acylates VLCPUFA to sphinganine, is highly expressed in meiotic cells. The Elovl4 and CerS3 protein expression prevails in spermatocytes, is seminiferous stage-specific, and is mostly concomitant. In spermatids, the Fa2h protein appears concentrated in late stages, especially when they elongate and their heads change shape. The unique sphingolipid species with n-V and h-V, as well as the enzymes involved in their biosynthesis, are useful biomarkers for investigating normal and pathological aspects of germ and sperm cell functions. Supported by SGCyT UNS-PGI-UNS (24/B272 to GMO and 24/B218 to MIA), FONCyT (PICT2017-2535 to GMO).
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
The LV Annual SAIB Meeting and XIV PABMB Congress
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Panamerican Association of Biochemestry and Molecular Biology
description The sphingomyelins (SM) and ceramides (Cer) of rodent spermatogenic cells contain very-long-chain (C28-C32) polyenoic fatty acids (VLCPUFA), in non-hydroxy (n-V) and 2-hydroxy (h-V) forms. The SM and Cer species with n-V, present in meiotic spermatocytes, become in part h-V species in post-meiotic spermatids. In each of these cells, the mentioned species are located in the non-raft fraction of the plasma membrane. The enzymes required for PUFA elongation to n-V, Elovl5, Elovl2 and Elovl4, and the fatty acid 2-hydroxylase (Fa2h) that converts n-V to h-V, are expressed in germ cells, with Elovl4 and Fa2h protein levels being highest in spermatocytes and spermatids, respectively. The Cer and SM species with n-V and h-V are biosynthesized de novo in a germ cell type-specific and steroid hormone-dependent manner. CerS3, which specifically N-acylates VLCPUFA to sphinganine, is highly expressed in meiotic cells. The Elovl4 and CerS3 protein expression prevails in spermatocytes, is seminiferous stage-specific, and is mostly concomitant. In spermatids, the Fa2h protein appears concentrated in late stages, especially when they elongate and their heads change shape. The unique sphingolipid species with n-V and h-V, as well as the enzymes involved in their biosynthesis, are useful biomarkers for investigating normal and pathological aspects of germ and sperm cell functions. Supported by SGCyT UNS-PGI-UNS (24/B272 to GMO and 24/B218 to MIA), FONCyT (PICT2017-2535 to GMO).
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/156614
Biosynthesis of sphingolipids with very-long-chain pufa a hallmark of differentiating male germ cells; The LV Annual SAIB Meeting and XIV PABMB Congress; Argentina; 2019; 34-34
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/156614
identifier_str_mv Biosynthesis of sphingolipids with very-long-chain pufa a hallmark of differentiating male germ cells; The LV Annual SAIB Meeting and XIV PABMB Congress; Argentina; 2019; 34-34
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/
info:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2019-version-final.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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