Luminal calcium concentration controls intestinal calcium absorption by modification of intestinal alkaline phosphatase activity

Autores
Brun, Lucas Ricardo Martín; Brance, María Lorena; Rigalli, Alfredo
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Intestinal alkaline phosphatase (IAP) is a brush-border phosphomonoesterase. Its location suggests an involvement in the uptake of nutrients, but its role has not yet been defined. IAP expression parallels that of other proteins involved in Ca absorption under vitamin D stimulation. Experiments carried out in vitro with purified IAP have demonstrated an interaction between Ca and IAP. The gut is prepared to face different levels of Ca intake over time, but high Ca intake in a situation of a low-Ca diet over time would cause excessive entry of Ca into the enterocytes. The presence of a mechanism to block Ca entry and to avoid possible adverse effects is thus predictable. Thus, in the present study, Sprague-Dawley rats were fed with different amounts of Ca in the diet (0•2, 1 and 2 g%), and the percentage of Ca absorption (%Ca) in the presence and absence of l-phenylalanine (Phe) was calculated. The presence of Phe caused a significant increase in %Ca (52•3 (SEM 6•5)% in the presence of Phe v. 31•1 (SEM 8•9)% in the absence of Phe, regardless of the amount of Ca intake; paired t test, P = 0•02). When data were analysed with respect to Ca intake, a significant difference was found only in the group with low Ca intake (paired t test, P = 0•03). Additionally, IAP activity increased significantly (ANOVA, P < 0•05) as Ca concentrations increased in the duodenal lumen. The present study provides in vivo evidence that luminal Ca concentration increases the activity of IAP and simultaneously decreases %Ca, acting as a minute-to-minute regulatory mechanism of Ca entry.
Fil: Brun, Lucas Ricardo Martín. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brance, María Lorena. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rigalli, Alfredo. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
CALCIUM
CALCIUM ABSORPTION
INTESTINAL ALKALINE PHOSPHATASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195893
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/195893
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Luminal calcium concentration controls intestinal calcium absorption by modification of intestinal alkaline phosphatase activityBrun, Lucas Ricardo MartínBrance, María LorenaRigalli, AlfredoCALCIUMCALCIUM ABSORPTIONINTESTINAL ALKALINE PHOSPHATASEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Intestinal alkaline phosphatase (IAP) is a brush-border phosphomonoesterase. Its location suggests an involvement in the uptake of nutrients, but its role has not yet been defined. IAP expression parallels that of other proteins involved in Ca absorption under vitamin D stimulation. Experiments carried out in vitro with purified IAP have demonstrated an interaction between Ca and IAP. The gut is prepared to face different levels of Ca intake over time, but high Ca intake in a situation of a low-Ca diet over time would cause excessive entry of Ca into the enterocytes. The presence of a mechanism to block Ca entry and to avoid possible adverse effects is thus predictable. Thus, in the present study, Sprague-Dawley rats were fed with different amounts of Ca in the diet (0•2, 1 and 2 g%), and the percentage of Ca absorption (%Ca) in the presence and absence of l-phenylalanine (Phe) was calculated. The presence of Phe caused a significant increase in %Ca (52•3 (SEM 6•5)% in the presence of Phe v. 31•1 (SEM 8•9)% in the absence of Phe, regardless of the amount of Ca intake; paired t test, P = 0•02). When data were analysed with respect to Ca intake, a significant difference was found only in the group with low Ca intake (paired t test, P = 0•03). Additionally, IAP activity increased significantly (ANOVA, P < 0•05) as Ca concentrations increased in the duodenal lumen. The present study provides in vivo evidence that luminal Ca concentration increases the activity of IAP and simultaneously decreases %Ca, acting as a minute-to-minute regulatory mechanism of Ca entry.Fil: Brun, Lucas Ricardo Martín. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Brance, María Lorena. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rigalli, Alfredo. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaCambridge University Press2012-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195893Brun, Lucas Ricardo Martín; Brance, María Lorena; Rigalli, Alfredo; Luminal calcium concentration controls intestinal calcium absorption by modification of intestinal alkaline phosphatase activity; Cambridge University Press; British Journal of Nutrition; 108; 2; 7-2012; 229-2330007-1145CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1017/S0007114511005617info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:25:25Zoai:ri.conicet.gov.ar:11336/195893instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:25:25.934CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Luminal calcium concentration controls intestinal calcium absorption by modification of intestinal alkaline phosphatase activity
title Luminal calcium concentration controls intestinal calcium absorption by modification of intestinal alkaline phosphatase activity
spellingShingle Luminal calcium concentration controls intestinal calcium absorption by modification of intestinal alkaline phosphatase activity
Brun, Lucas Ricardo Martín
CALCIUM
CALCIUM ABSORPTION
INTESTINAL ALKALINE PHOSPHATASE
title_short Luminal calcium concentration controls intestinal calcium absorption by modification of intestinal alkaline phosphatase activity
title_full Luminal calcium concentration controls intestinal calcium absorption by modification of intestinal alkaline phosphatase activity
title_fullStr Luminal calcium concentration controls intestinal calcium absorption by modification of intestinal alkaline phosphatase activity
title_full_unstemmed Luminal calcium concentration controls intestinal calcium absorption by modification of intestinal alkaline phosphatase activity
title_sort Luminal calcium concentration controls intestinal calcium absorption by modification of intestinal alkaline phosphatase activity
dc.creator.none.fl_str_mv Brun, Lucas Ricardo Martín
Brance, María Lorena
Rigalli, Alfredo
author Brun, Lucas Ricardo Martín
author_facet Brun, Lucas Ricardo Martín
Brance, María Lorena
Rigalli, Alfredo
author_role author
author2 Brance, María Lorena
Rigalli, Alfredo
author2_role author
author
dc.subject.none.fl_str_mv CALCIUM
CALCIUM ABSORPTION
INTESTINAL ALKALINE PHOSPHATASE
topic CALCIUM
CALCIUM ABSORPTION
INTESTINAL ALKALINE PHOSPHATASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Intestinal alkaline phosphatase (IAP) is a brush-border phosphomonoesterase. Its location suggests an involvement in the uptake of nutrients, but its role has not yet been defined. IAP expression parallels that of other proteins involved in Ca absorption under vitamin D stimulation. Experiments carried out in vitro with purified IAP have demonstrated an interaction between Ca and IAP. The gut is prepared to face different levels of Ca intake over time, but high Ca intake in a situation of a low-Ca diet over time would cause excessive entry of Ca into the enterocytes. The presence of a mechanism to block Ca entry and to avoid possible adverse effects is thus predictable. Thus, in the present study, Sprague-Dawley rats were fed with different amounts of Ca in the diet (0•2, 1 and 2 g%), and the percentage of Ca absorption (%Ca) in the presence and absence of l-phenylalanine (Phe) was calculated. The presence of Phe caused a significant increase in %Ca (52•3 (SEM 6•5)% in the presence of Phe v. 31•1 (SEM 8•9)% in the absence of Phe, regardless of the amount of Ca intake; paired t test, P = 0•02). When data were analysed with respect to Ca intake, a significant difference was found only in the group with low Ca intake (paired t test, P = 0•03). Additionally, IAP activity increased significantly (ANOVA, P < 0•05) as Ca concentrations increased in the duodenal lumen. The present study provides in vivo evidence that luminal Ca concentration increases the activity of IAP and simultaneously decreases %Ca, acting as a minute-to-minute regulatory mechanism of Ca entry.
Fil: Brun, Lucas Ricardo Martín. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brance, María Lorena. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rigalli, Alfredo. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Intestinal alkaline phosphatase (IAP) is a brush-border phosphomonoesterase. Its location suggests an involvement in the uptake of nutrients, but its role has not yet been defined. IAP expression parallels that of other proteins involved in Ca absorption under vitamin D stimulation. Experiments carried out in vitro with purified IAP have demonstrated an interaction between Ca and IAP. The gut is prepared to face different levels of Ca intake over time, but high Ca intake in a situation of a low-Ca diet over time would cause excessive entry of Ca into the enterocytes. The presence of a mechanism to block Ca entry and to avoid possible adverse effects is thus predictable. Thus, in the present study, Sprague-Dawley rats were fed with different amounts of Ca in the diet (0•2, 1 and 2 g%), and the percentage of Ca absorption (%Ca) in the presence and absence of l-phenylalanine (Phe) was calculated. The presence of Phe caused a significant increase in %Ca (52•3 (SEM 6•5)% in the presence of Phe v. 31•1 (SEM 8•9)% in the absence of Phe, regardless of the amount of Ca intake; paired t test, P = 0•02). When data were analysed with respect to Ca intake, a significant difference was found only in the group with low Ca intake (paired t test, P = 0•03). Additionally, IAP activity increased significantly (ANOVA, P < 0•05) as Ca concentrations increased in the duodenal lumen. The present study provides in vivo evidence that luminal Ca concentration increases the activity of IAP and simultaneously decreases %Ca, acting as a minute-to-minute regulatory mechanism of Ca entry.
publishDate 2012
dc.date.none.fl_str_mv 2012-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195893
Brun, Lucas Ricardo Martín; Brance, María Lorena; Rigalli, Alfredo; Luminal calcium concentration controls intestinal calcium absorption by modification of intestinal alkaline phosphatase activity; Cambridge University Press; British Journal of Nutrition; 108; 2; 7-2012; 229-233
0007-1145
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195893
identifier_str_mv Brun, Lucas Ricardo Martín; Brance, María Lorena; Rigalli, Alfredo; Luminal calcium concentration controls intestinal calcium absorption by modification of intestinal alkaline phosphatase activity; Cambridge University Press; British Journal of Nutrition; 108; 2; 7-2012; 229-233
0007-1145
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1017/S0007114511005617
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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