Luminal calcium concentration controls intestinal calcium absorption by modification of intestinal alkaline phosphatase activity
- Autores
- Brun, Lucas Ricardo Martín; Brance, María Lorena; Rigalli, Alfredo
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Intestinal alkaline phosphatase (IAP) is a brush-border phosphomonoesterase. Its location suggests an involvement in the uptake of nutrients, but its role has not yet been defined. IAP expression parallels that of other proteins involved in Ca absorption under vitamin D stimulation. Experiments carried out in vitro with purified IAP have demonstrated an interaction between Ca and IAP. The gut is prepared to face different levels of Ca intake over time, but high Ca intake in a situation of a low-Ca diet over time would cause excessive entry of Ca into the enterocytes. The presence of a mechanism to block Ca entry and to avoid possible adverse effects is thus predictable. Thus, in the present study, Sprague-Dawley rats were fed with different amounts of Ca in the diet (0•2, 1 and 2 g%), and the percentage of Ca absorption (%Ca) in the presence and absence of l-phenylalanine (Phe) was calculated. The presence of Phe caused a significant increase in %Ca (52•3 (SEM 6•5)% in the presence of Phe v. 31•1 (SEM 8•9)% in the absence of Phe, regardless of the amount of Ca intake; paired t test, P = 0•02). When data were analysed with respect to Ca intake, a significant difference was found only in the group with low Ca intake (paired t test, P = 0•03). Additionally, IAP activity increased significantly (ANOVA, P < 0•05) as Ca concentrations increased in the duodenal lumen. The present study provides in vivo evidence that luminal Ca concentration increases the activity of IAP and simultaneously decreases %Ca, acting as a minute-to-minute regulatory mechanism of Ca entry.
Fil: Brun, Lucas Ricardo Martín. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brance, María Lorena. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rigalli, Alfredo. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
CALCIUM
CALCIUM ABSORPTION
INTESTINAL ALKALINE PHOSPHATASE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/195893
Ver los metadatos del registro completo
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Luminal calcium concentration controls intestinal calcium absorption by modification of intestinal alkaline phosphatase activityBrun, Lucas Ricardo MartínBrance, María LorenaRigalli, AlfredoCALCIUMCALCIUM ABSORPTIONINTESTINAL ALKALINE PHOSPHATASEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Intestinal alkaline phosphatase (IAP) is a brush-border phosphomonoesterase. Its location suggests an involvement in the uptake of nutrients, but its role has not yet been defined. IAP expression parallels that of other proteins involved in Ca absorption under vitamin D stimulation. Experiments carried out in vitro with purified IAP have demonstrated an interaction between Ca and IAP. The gut is prepared to face different levels of Ca intake over time, but high Ca intake in a situation of a low-Ca diet over time would cause excessive entry of Ca into the enterocytes. The presence of a mechanism to block Ca entry and to avoid possible adverse effects is thus predictable. Thus, in the present study, Sprague-Dawley rats were fed with different amounts of Ca in the diet (0•2, 1 and 2 g%), and the percentage of Ca absorption (%Ca) in the presence and absence of l-phenylalanine (Phe) was calculated. The presence of Phe caused a significant increase in %Ca (52•3 (SEM 6•5)% in the presence of Phe v. 31•1 (SEM 8•9)% in the absence of Phe, regardless of the amount of Ca intake; paired t test, P = 0•02). When data were analysed with respect to Ca intake, a significant difference was found only in the group with low Ca intake (paired t test, P = 0•03). Additionally, IAP activity increased significantly (ANOVA, P < 0•05) as Ca concentrations increased in the duodenal lumen. The present study provides in vivo evidence that luminal Ca concentration increases the activity of IAP and simultaneously decreases %Ca, acting as a minute-to-minute regulatory mechanism of Ca entry.Fil: Brun, Lucas Ricardo Martín. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Brance, María Lorena. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rigalli, Alfredo. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaCambridge University Press2012-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195893Brun, Lucas Ricardo Martín; Brance, María Lorena; Rigalli, Alfredo; Luminal calcium concentration controls intestinal calcium absorption by modification of intestinal alkaline phosphatase activity; Cambridge University Press; British Journal of Nutrition; 108; 2; 7-2012; 229-2330007-1145CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1017/S0007114511005617info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:04Zoai:ri.conicet.gov.ar:11336/195893instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:04.56CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Luminal calcium concentration controls intestinal calcium absorption by modification of intestinal alkaline phosphatase activity |
title |
Luminal calcium concentration controls intestinal calcium absorption by modification of intestinal alkaline phosphatase activity |
spellingShingle |
Luminal calcium concentration controls intestinal calcium absorption by modification of intestinal alkaline phosphatase activity Brun, Lucas Ricardo Martín CALCIUM CALCIUM ABSORPTION INTESTINAL ALKALINE PHOSPHATASE |
title_short |
Luminal calcium concentration controls intestinal calcium absorption by modification of intestinal alkaline phosphatase activity |
title_full |
Luminal calcium concentration controls intestinal calcium absorption by modification of intestinal alkaline phosphatase activity |
title_fullStr |
Luminal calcium concentration controls intestinal calcium absorption by modification of intestinal alkaline phosphatase activity |
title_full_unstemmed |
Luminal calcium concentration controls intestinal calcium absorption by modification of intestinal alkaline phosphatase activity |
title_sort |
Luminal calcium concentration controls intestinal calcium absorption by modification of intestinal alkaline phosphatase activity |
dc.creator.none.fl_str_mv |
Brun, Lucas Ricardo Martín Brance, María Lorena Rigalli, Alfredo |
author |
Brun, Lucas Ricardo Martín |
author_facet |
Brun, Lucas Ricardo Martín Brance, María Lorena Rigalli, Alfredo |
author_role |
author |
author2 |
Brance, María Lorena Rigalli, Alfredo |
author2_role |
author author |
dc.subject.none.fl_str_mv |
CALCIUM CALCIUM ABSORPTION INTESTINAL ALKALINE PHOSPHATASE |
topic |
CALCIUM CALCIUM ABSORPTION INTESTINAL ALKALINE PHOSPHATASE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Intestinal alkaline phosphatase (IAP) is a brush-border phosphomonoesterase. Its location suggests an involvement in the uptake of nutrients, but its role has not yet been defined. IAP expression parallels that of other proteins involved in Ca absorption under vitamin D stimulation. Experiments carried out in vitro with purified IAP have demonstrated an interaction between Ca and IAP. The gut is prepared to face different levels of Ca intake over time, but high Ca intake in a situation of a low-Ca diet over time would cause excessive entry of Ca into the enterocytes. The presence of a mechanism to block Ca entry and to avoid possible adverse effects is thus predictable. Thus, in the present study, Sprague-Dawley rats were fed with different amounts of Ca in the diet (0•2, 1 and 2 g%), and the percentage of Ca absorption (%Ca) in the presence and absence of l-phenylalanine (Phe) was calculated. The presence of Phe caused a significant increase in %Ca (52•3 (SEM 6•5)% in the presence of Phe v. 31•1 (SEM 8•9)% in the absence of Phe, regardless of the amount of Ca intake; paired t test, P = 0•02). When data were analysed with respect to Ca intake, a significant difference was found only in the group with low Ca intake (paired t test, P = 0•03). Additionally, IAP activity increased significantly (ANOVA, P < 0•05) as Ca concentrations increased in the duodenal lumen. The present study provides in vivo evidence that luminal Ca concentration increases the activity of IAP and simultaneously decreases %Ca, acting as a minute-to-minute regulatory mechanism of Ca entry. Fil: Brun, Lucas Ricardo Martín. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Brance, María Lorena. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rigalli, Alfredo. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
Intestinal alkaline phosphatase (IAP) is a brush-border phosphomonoesterase. Its location suggests an involvement in the uptake of nutrients, but its role has not yet been defined. IAP expression parallels that of other proteins involved in Ca absorption under vitamin D stimulation. Experiments carried out in vitro with purified IAP have demonstrated an interaction between Ca and IAP. The gut is prepared to face different levels of Ca intake over time, but high Ca intake in a situation of a low-Ca diet over time would cause excessive entry of Ca into the enterocytes. The presence of a mechanism to block Ca entry and to avoid possible adverse effects is thus predictable. Thus, in the present study, Sprague-Dawley rats were fed with different amounts of Ca in the diet (0•2, 1 and 2 g%), and the percentage of Ca absorption (%Ca) in the presence and absence of l-phenylalanine (Phe) was calculated. The presence of Phe caused a significant increase in %Ca (52•3 (SEM 6•5)% in the presence of Phe v. 31•1 (SEM 8•9)% in the absence of Phe, regardless of the amount of Ca intake; paired t test, P = 0•02). When data were analysed with respect to Ca intake, a significant difference was found only in the group with low Ca intake (paired t test, P = 0•03). Additionally, IAP activity increased significantly (ANOVA, P < 0•05) as Ca concentrations increased in the duodenal lumen. The present study provides in vivo evidence that luminal Ca concentration increases the activity of IAP and simultaneously decreases %Ca, acting as a minute-to-minute regulatory mechanism of Ca entry. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/195893 Brun, Lucas Ricardo Martín; Brance, María Lorena; Rigalli, Alfredo; Luminal calcium concentration controls intestinal calcium absorption by modification of intestinal alkaline phosphatase activity; Cambridge University Press; British Journal of Nutrition; 108; 2; 7-2012; 229-233 0007-1145 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/195893 |
identifier_str_mv |
Brun, Lucas Ricardo Martín; Brance, María Lorena; Rigalli, Alfredo; Luminal calcium concentration controls intestinal calcium absorption by modification of intestinal alkaline phosphatase activity; Cambridge University Press; British Journal of Nutrition; 108; 2; 7-2012; 229-233 0007-1145 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1017/S0007114511005617 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Cambridge University Press |
publisher.none.fl_str_mv |
Cambridge University Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269890313977856 |
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13.13397 |