Regulation of intestinal calcium absorption by luminal calcium content. Role of intestinal alkaline phosphatase

Autores
Brun, Lucas Ricardo Martín; Brance, María Lorena; Lombarte, Mercedes; Lupo, Maela; Di Loreto, Verónica Elina; Rigalli, Alfredo
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Scope: Intestinal alkaline phosphatase is a brush border enzyme that is stimulated by calcium. Inhibition of intestinal alkaline phosphatase increases intestinal calcium absorption. We hypothesized that intestinal alkaline phosphatase acts as a minute-to-minute regulatory mechanism of calcium entry. The aim of this study was to evaluate the mechanism by which intestinal luminal calcium controls intestinal calcium absorption. Methods and results: We performed kinetic studies with purified intestinal alkaline phosphatase and everted duodenal sacs and showed that intestinal alkaline phosphatase modifies the luminal pH as a function of enzyme concentration and calcium luminal content. A decrease in pH occurred simultaneously with a decrease in calcium absorption. The inhibition of intestinal alkaline phosphatase by L-phenylalanine caused an increase in calcium absorption. This effect was also confirmed in calcium uptake experiments with isolated duodenal cells. Conclusion: Changes in luminal pH arising from intestinal alkaline phosphatase activity induced by luminal calcium concentration modulate intestinal calcium absorption.
Fil: Brun, Lucas Ricardo Martín. Universidad Nacional de Rosario. Facultad de Cs.médicas. Escuela de Cs.médicas. Cátedra de Química Biologica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Brance, María Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Cs.médicas. Escuela de Cs.médicas. Cátedra de Química Biologica; Argentina
Fil: Lombarte, Mercedes. Universidad Nacional de Rosario. Facultad de Cs.médicas. Escuela de Cs.médicas. Cátedra de Química Biologica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Lupo, Maela. Universidad Nacional de Rosario. Facultad de Cs.médicas. Escuela de Cs.médicas. Cátedra de Química Biologica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Di Loreto, Verónica Elina. Universidad Nacional de Rosario. Facultad de Cs.médicas. Escuela de Cs.médicas. Cátedra de Química Biologica; Argentina
Fil: Rigalli, Alfredo. Universidad Nacional de Rosario. Facultad de Cs.médicas. Escuela de Cs.médicas. Cátedra de Química Biologica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Materia
CALCIUM
CALCIUM UPTAKE
INTESTINAL ALKALINE PHOSPHATASE
LUMINAL CALCIUM CONTENT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/29914
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/29914
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Regulation of intestinal calcium absorption by luminal calcium content. Role of intestinal alkaline phosphataseBrun, Lucas Ricardo MartínBrance, María LorenaLombarte, MercedesLupo, MaelaDi Loreto, Verónica ElinaRigalli, AlfredoCALCIUMCALCIUM UPTAKEINTESTINAL ALKALINE PHOSPHATASELUMINAL CALCIUM CONTENThttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Scope: Intestinal alkaline phosphatase is a brush border enzyme that is stimulated by calcium. Inhibition of intestinal alkaline phosphatase increases intestinal calcium absorption. We hypothesized that intestinal alkaline phosphatase acts as a minute-to-minute regulatory mechanism of calcium entry. The aim of this study was to evaluate the mechanism by which intestinal luminal calcium controls intestinal calcium absorption. Methods and results: We performed kinetic studies with purified intestinal alkaline phosphatase and everted duodenal sacs and showed that intestinal alkaline phosphatase modifies the luminal pH as a function of enzyme concentration and calcium luminal content. A decrease in pH occurred simultaneously with a decrease in calcium absorption. The inhibition of intestinal alkaline phosphatase by L-phenylalanine caused an increase in calcium absorption. This effect was also confirmed in calcium uptake experiments with isolated duodenal cells. Conclusion: Changes in luminal pH arising from intestinal alkaline phosphatase activity induced by luminal calcium concentration modulate intestinal calcium absorption.Fil: Brun, Lucas Ricardo Martín. Universidad Nacional de Rosario. Facultad de Cs.médicas. Escuela de Cs.médicas. Cátedra de Química Biologica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Brance, María Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Cs.médicas. Escuela de Cs.médicas. Cátedra de Química Biologica; ArgentinaFil: Lombarte, Mercedes. Universidad Nacional de Rosario. Facultad de Cs.médicas. Escuela de Cs.médicas. Cátedra de Química Biologica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Lupo, Maela. Universidad Nacional de Rosario. Facultad de Cs.médicas. Escuela de Cs.médicas. Cátedra de Química Biologica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Di Loreto, Verónica Elina. Universidad Nacional de Rosario. Facultad de Cs.médicas. Escuela de Cs.médicas. Cátedra de Química Biologica; ArgentinaFil: Rigalli, Alfredo. Universidad Nacional de Rosario. Facultad de Cs.médicas. Escuela de Cs.médicas. Cátedra de Química Biologica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaWiley VCH Verlag2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/29914Brun, Lucas Ricardo Martín; Brance, María Lorena; Lombarte, Mercedes; Lupo, Maela; Di Loreto, Verónica Elina; et al.; Regulation of intestinal calcium absorption by luminal calcium content. Role of intestinal alkaline phosphatase; Wiley VCH Verlag; Molecular Nutrition & Food Research; 58; 7; 3-2014; 1546-15511613-4125CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/mnfr.201300686/pdfinfo:eu-repo/semantics/altIdentifier/doi/10.1002/mnfr.201300686info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:51:08Zoai:ri.conicet.gov.ar:11336/29914instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:51:09.077CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Regulation of intestinal calcium absorption by luminal calcium content. Role of intestinal alkaline phosphatase
title Regulation of intestinal calcium absorption by luminal calcium content. Role of intestinal alkaline phosphatase
spellingShingle Regulation of intestinal calcium absorption by luminal calcium content. Role of intestinal alkaline phosphatase
Brun, Lucas Ricardo Martín
CALCIUM
CALCIUM UPTAKE
INTESTINAL ALKALINE PHOSPHATASE
LUMINAL CALCIUM CONTENT
title_short Regulation of intestinal calcium absorption by luminal calcium content. Role of intestinal alkaline phosphatase
title_full Regulation of intestinal calcium absorption by luminal calcium content. Role of intestinal alkaline phosphatase
title_fullStr Regulation of intestinal calcium absorption by luminal calcium content. Role of intestinal alkaline phosphatase
title_full_unstemmed Regulation of intestinal calcium absorption by luminal calcium content. Role of intestinal alkaline phosphatase
title_sort Regulation of intestinal calcium absorption by luminal calcium content. Role of intestinal alkaline phosphatase
dc.creator.none.fl_str_mv Brun, Lucas Ricardo Martín
Brance, María Lorena
Lombarte, Mercedes
Lupo, Maela
Di Loreto, Verónica Elina
Rigalli, Alfredo
author Brun, Lucas Ricardo Martín
author_facet Brun, Lucas Ricardo Martín
Brance, María Lorena
Lombarte, Mercedes
Lupo, Maela
Di Loreto, Verónica Elina
Rigalli, Alfredo
author_role author
author2 Brance, María Lorena
Lombarte, Mercedes
Lupo, Maela
Di Loreto, Verónica Elina
Rigalli, Alfredo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CALCIUM
CALCIUM UPTAKE
INTESTINAL ALKALINE PHOSPHATASE
LUMINAL CALCIUM CONTENT
topic CALCIUM
CALCIUM UPTAKE
INTESTINAL ALKALINE PHOSPHATASE
LUMINAL CALCIUM CONTENT
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Scope: Intestinal alkaline phosphatase is a brush border enzyme that is stimulated by calcium. Inhibition of intestinal alkaline phosphatase increases intestinal calcium absorption. We hypothesized that intestinal alkaline phosphatase acts as a minute-to-minute regulatory mechanism of calcium entry. The aim of this study was to evaluate the mechanism by which intestinal luminal calcium controls intestinal calcium absorption. Methods and results: We performed kinetic studies with purified intestinal alkaline phosphatase and everted duodenal sacs and showed that intestinal alkaline phosphatase modifies the luminal pH as a function of enzyme concentration and calcium luminal content. A decrease in pH occurred simultaneously with a decrease in calcium absorption. The inhibition of intestinal alkaline phosphatase by L-phenylalanine caused an increase in calcium absorption. This effect was also confirmed in calcium uptake experiments with isolated duodenal cells. Conclusion: Changes in luminal pH arising from intestinal alkaline phosphatase activity induced by luminal calcium concentration modulate intestinal calcium absorption.
Fil: Brun, Lucas Ricardo Martín. Universidad Nacional de Rosario. Facultad de Cs.médicas. Escuela de Cs.médicas. Cátedra de Química Biologica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Brance, María Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Cs.médicas. Escuela de Cs.médicas. Cátedra de Química Biologica; Argentina
Fil: Lombarte, Mercedes. Universidad Nacional de Rosario. Facultad de Cs.médicas. Escuela de Cs.médicas. Cátedra de Química Biologica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Lupo, Maela. Universidad Nacional de Rosario. Facultad de Cs.médicas. Escuela de Cs.médicas. Cátedra de Química Biologica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Di Loreto, Verónica Elina. Universidad Nacional de Rosario. Facultad de Cs.médicas. Escuela de Cs.médicas. Cátedra de Química Biologica; Argentina
Fil: Rigalli, Alfredo. Universidad Nacional de Rosario. Facultad de Cs.médicas. Escuela de Cs.médicas. Cátedra de Química Biologica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
description Scope: Intestinal alkaline phosphatase is a brush border enzyme that is stimulated by calcium. Inhibition of intestinal alkaline phosphatase increases intestinal calcium absorption. We hypothesized that intestinal alkaline phosphatase acts as a minute-to-minute regulatory mechanism of calcium entry. The aim of this study was to evaluate the mechanism by which intestinal luminal calcium controls intestinal calcium absorption. Methods and results: We performed kinetic studies with purified intestinal alkaline phosphatase and everted duodenal sacs and showed that intestinal alkaline phosphatase modifies the luminal pH as a function of enzyme concentration and calcium luminal content. A decrease in pH occurred simultaneously with a decrease in calcium absorption. The inhibition of intestinal alkaline phosphatase by L-phenylalanine caused an increase in calcium absorption. This effect was also confirmed in calcium uptake experiments with isolated duodenal cells. Conclusion: Changes in luminal pH arising from intestinal alkaline phosphatase activity induced by luminal calcium concentration modulate intestinal calcium absorption.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/29914
Brun, Lucas Ricardo Martín; Brance, María Lorena; Lombarte, Mercedes; Lupo, Maela; Di Loreto, Verónica Elina; et al.; Regulation of intestinal calcium absorption by luminal calcium content. Role of intestinal alkaline phosphatase; Wiley VCH Verlag; Molecular Nutrition & Food Research; 58; 7; 3-2014; 1546-1551
1613-4125
CONICET Digital
CONICET
url http://hdl.handle.net/11336/29914
identifier_str_mv Brun, Lucas Ricardo Martín; Brance, María Lorena; Lombarte, Mercedes; Lupo, Maela; Di Loreto, Verónica Elina; et al.; Regulation of intestinal calcium absorption by luminal calcium content. Role of intestinal alkaline phosphatase; Wiley VCH Verlag; Molecular Nutrition & Food Research; 58; 7; 3-2014; 1546-1551
1613-4125
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/mnfr.201300686/pdf
info:eu-repo/semantics/altIdentifier/doi/10.1002/mnfr.201300686
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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