Increased calcium uptake and improved trabecular bone properties in intestinal alkaline phosphatase knockout mice

Autores
Brun, Lucas Ricardo Martín; Lombarte, Mercedes; Roma, S; Perez, F.; Millán, José Luis; Rigalli, Alfredo
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Previous studies have demonstrated a negative correlation between intestinal alkaline phosphatase (IAP) activity and calcium (Ca) absorption in the gut, as IAP acts as a protective mechanism inhibiting high Ca entry into enterocytes, preventing Ca overload. Here we evaluated Ca absorption and bone properties in knockout mice (KO) completely devoid of duodenal IAP (Akp3−/− mice). Female C57BL/6 control mice (WT, n = 7) and KO mice (n = 10) were used to determine Ca absorption in vivo and by in situ isolated duodenal loops followed by histomorphometric analysis of duodenal villi and crypts. Bone mineral density, morphometry, histomorphometry and trabecular connectivity and biomechanical properties were measured on bones. We observed mild atrophy of the villi with lower absorption surface and a significantly higher Ca uptake in KO mice. While no changes were seen in cortical bone, we found better trabecular connectivity and biomechanical properties in the femurs of KO mice compared to WT mice. Our data indicate that IAP KO mice display higher intestinal Ca uptake, which over time appears to correlate with a positive effect on the biomechanical properties of trabecular bone.
Fil: Brun, Lucas Ricardo Martín. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lombarte, Mercedes. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Roma, S. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina
Fil: Perez, F.. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina
Fil: Millán, José Luis. Sanford Burnham Prebys Medical Discovery Institute; Estados Unidos
Fil: Rigalli, Alfredo. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Bone Properties
Calcium Absorption
Intestinal Alkaline Phosphatase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/50353
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Increased calcium uptake and improved trabecular bone properties in intestinal alkaline phosphatase knockout miceBrun, Lucas Ricardo MartínLombarte, MercedesRoma, SPerez, F.Millán, José LuisRigalli, AlfredoBone PropertiesCalcium AbsorptionIntestinal Alkaline Phosphatasehttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Previous studies have demonstrated a negative correlation between intestinal alkaline phosphatase (IAP) activity and calcium (Ca) absorption in the gut, as IAP acts as a protective mechanism inhibiting high Ca entry into enterocytes, preventing Ca overload. Here we evaluated Ca absorption and bone properties in knockout mice (KO) completely devoid of duodenal IAP (Akp3−/− mice). Female C57BL/6 control mice (WT, n = 7) and KO mice (n = 10) were used to determine Ca absorption in vivo and by in situ isolated duodenal loops followed by histomorphometric analysis of duodenal villi and crypts. Bone mineral density, morphometry, histomorphometry and trabecular connectivity and biomechanical properties were measured on bones. We observed mild atrophy of the villi with lower absorption surface and a significantly higher Ca uptake in KO mice. While no changes were seen in cortical bone, we found better trabecular connectivity and biomechanical properties in the femurs of KO mice compared to WT mice. Our data indicate that IAP KO mice display higher intestinal Ca uptake, which over time appears to correlate with a positive effect on the biomechanical properties of trabecular bone.Fil: Brun, Lucas Ricardo Martín. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lombarte, Mercedes. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Roma, S. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; ArgentinaFil: Perez, F.. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; ArgentinaFil: Millán, José Luis. Sanford Burnham Prebys Medical Discovery Institute; Estados UnidosFil: Rigalli, Alfredo. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer Tokyo2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/50353Brun, Lucas Ricardo Martín; Lombarte, Mercedes; Roma, S; Perez, F.; Millán, José Luis; et al.; Increased calcium uptake and improved trabecular bone properties in intestinal alkaline phosphatase knockout mice; Springer Tokyo; Journal Of Bone And Mineral Metabolism.; 12-2017; 1-70914-87791435-5604CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00774-017-0887-7info:eu-repo/semantics/altIdentifier/doi/10.1007/s00774-017-0887-7info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6338327/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:04Zoai:ri.conicet.gov.ar:11336/50353instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:04.868CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Increased calcium uptake and improved trabecular bone properties in intestinal alkaline phosphatase knockout mice
title Increased calcium uptake and improved trabecular bone properties in intestinal alkaline phosphatase knockout mice
spellingShingle Increased calcium uptake and improved trabecular bone properties in intestinal alkaline phosphatase knockout mice
Brun, Lucas Ricardo Martín
Bone Properties
Calcium Absorption
Intestinal Alkaline Phosphatase
title_short Increased calcium uptake and improved trabecular bone properties in intestinal alkaline phosphatase knockout mice
title_full Increased calcium uptake and improved trabecular bone properties in intestinal alkaline phosphatase knockout mice
title_fullStr Increased calcium uptake and improved trabecular bone properties in intestinal alkaline phosphatase knockout mice
title_full_unstemmed Increased calcium uptake and improved trabecular bone properties in intestinal alkaline phosphatase knockout mice
title_sort Increased calcium uptake and improved trabecular bone properties in intestinal alkaline phosphatase knockout mice
dc.creator.none.fl_str_mv Brun, Lucas Ricardo Martín
Lombarte, Mercedes
Roma, S
Perez, F.
Millán, José Luis
Rigalli, Alfredo
author Brun, Lucas Ricardo Martín
author_facet Brun, Lucas Ricardo Martín
Lombarte, Mercedes
Roma, S
Perez, F.
Millán, José Luis
Rigalli, Alfredo
author_role author
author2 Lombarte, Mercedes
Roma, S
Perez, F.
Millán, José Luis
Rigalli, Alfredo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bone Properties
Calcium Absorption
Intestinal Alkaline Phosphatase
topic Bone Properties
Calcium Absorption
Intestinal Alkaline Phosphatase
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Previous studies have demonstrated a negative correlation between intestinal alkaline phosphatase (IAP) activity and calcium (Ca) absorption in the gut, as IAP acts as a protective mechanism inhibiting high Ca entry into enterocytes, preventing Ca overload. Here we evaluated Ca absorption and bone properties in knockout mice (KO) completely devoid of duodenal IAP (Akp3−/− mice). Female C57BL/6 control mice (WT, n = 7) and KO mice (n = 10) were used to determine Ca absorption in vivo and by in situ isolated duodenal loops followed by histomorphometric analysis of duodenal villi and crypts. Bone mineral density, morphometry, histomorphometry and trabecular connectivity and biomechanical properties were measured on bones. We observed mild atrophy of the villi with lower absorption surface and a significantly higher Ca uptake in KO mice. While no changes were seen in cortical bone, we found better trabecular connectivity and biomechanical properties in the femurs of KO mice compared to WT mice. Our data indicate that IAP KO mice display higher intestinal Ca uptake, which over time appears to correlate with a positive effect on the biomechanical properties of trabecular bone.
Fil: Brun, Lucas Ricardo Martín. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lombarte, Mercedes. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Roma, S. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina
Fil: Perez, F.. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina
Fil: Millán, José Luis. Sanford Burnham Prebys Medical Discovery Institute; Estados Unidos
Fil: Rigalli, Alfredo. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Previous studies have demonstrated a negative correlation between intestinal alkaline phosphatase (IAP) activity and calcium (Ca) absorption in the gut, as IAP acts as a protective mechanism inhibiting high Ca entry into enterocytes, preventing Ca overload. Here we evaluated Ca absorption and bone properties in knockout mice (KO) completely devoid of duodenal IAP (Akp3−/− mice). Female C57BL/6 control mice (WT, n = 7) and KO mice (n = 10) were used to determine Ca absorption in vivo and by in situ isolated duodenal loops followed by histomorphometric analysis of duodenal villi and crypts. Bone mineral density, morphometry, histomorphometry and trabecular connectivity and biomechanical properties were measured on bones. We observed mild atrophy of the villi with lower absorption surface and a significantly higher Ca uptake in KO mice. While no changes were seen in cortical bone, we found better trabecular connectivity and biomechanical properties in the femurs of KO mice compared to WT mice. Our data indicate that IAP KO mice display higher intestinal Ca uptake, which over time appears to correlate with a positive effect on the biomechanical properties of trabecular bone.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/50353
Brun, Lucas Ricardo Martín; Lombarte, Mercedes; Roma, S; Perez, F.; Millán, José Luis; et al.; Increased calcium uptake and improved trabecular bone properties in intestinal alkaline phosphatase knockout mice; Springer Tokyo; Journal Of Bone And Mineral Metabolism.; 12-2017; 1-7
0914-8779
1435-5604
CONICET Digital
CONICET
url http://hdl.handle.net/11336/50353
identifier_str_mv Brun, Lucas Ricardo Martín; Lombarte, Mercedes; Roma, S; Perez, F.; Millán, José Luis; et al.; Increased calcium uptake and improved trabecular bone properties in intestinal alkaline phosphatase knockout mice; Springer Tokyo; Journal Of Bone And Mineral Metabolism.; 12-2017; 1-7
0914-8779
1435-5604
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00774-017-0887-7
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00774-017-0887-7
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6338327/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Tokyo
publisher.none.fl_str_mv Springer Tokyo
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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