Recruitment of Orc6l, a dormant maternal mRNA in mouse oocytes, is essential for DNA replication in 1-cell embryos

Autores
Murai, Shin; Stein, Paula; Buffone, Mariano Gabriel; Yamashita, Shigeru; Schultz, Richard M.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mouse oocytes acquire the ability to replicate DNA during meiotic maturation, presumably to ensure that DNA replication does not occur precociously between MI and MII and only after fertilization. Acquisition of DNA replication competence requires protein synthesis, but the identity of the proteins required for DNA replication is poorly described. In Xenopus, the only component missing for DNA replication competence is CDC6, which is synthesized from a dormant maternal mRNA recruited during oocyte maturation, and a similar situation also occurs during mouse oocyte maturation. We report that ORC6L is another component required for acquisition of DNA replication competence that is absent in mouse oocytes. The dormant maternal Orc6l mRNA is recruited during maturation via a CPE present in its 3␣ UTR. RNAi-mediated ablation of maternal Orc6l mRNA prevents the maturation-associated increase in ORC6L protein and inhibits DNA replication in 1-cell embryos. These results suggest that mammalian oocytes have more complex mecha- nisms to establish DNA replication competence when compared to their Xenopus counterparts.
Fil: Murai, Shin. State University Of Pennsylvania; Estados Unidos
Fil: Stein, Paula. University of Pennsylvania; Estados Unidos
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; Argentina. University of Pennsylvania; Estados Unidos
Fil: Yamashita, Shigeru. Toho University. School of Medicine; Japón
Fil: Schultz, Richard M.. University of Pennsylvania; Estados Unidos
Materia
Dna Replication
Dormant Maternal Mrna
Cytoplasmic Poladenylation Element
Mouse 1-Cell Embryo
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14247
Acceder
id CONICETDig_f1766509d7c910989dca8d7c08323b20
oai_identifier_str oai:ri.conicet.gov.ar:11336/14247
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Recruitment of Orc6l, a dormant maternal mRNA in mouse oocytes, is essential for DNA replication in 1-cell embryosMurai, ShinStein, PaulaBuffone, Mariano GabrielYamashita, ShigeruSchultz, Richard M.Dna ReplicationDormant Maternal MrnaCytoplasmic Poladenylation ElementMouse 1-Cell Embryohttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Mouse oocytes acquire the ability to replicate DNA during meiotic maturation, presumably to ensure that DNA replication does not occur precociously between MI and MII and only after fertilization. Acquisition of DNA replication competence requires protein synthesis, but the identity of the proteins required for DNA replication is poorly described. In Xenopus, the only component missing for DNA replication competence is CDC6, which is synthesized from a dormant maternal mRNA recruited during oocyte maturation, and a similar situation also occurs during mouse oocyte maturation. We report that ORC6L is another component required for acquisition of DNA replication competence that is absent in mouse oocytes. The dormant maternal Orc6l mRNA is recruited during maturation via a CPE present in its 3␣ UTR. RNAi-mediated ablation of maternal Orc6l mRNA prevents the maturation-associated increase in ORC6L protein and inhibits DNA replication in 1-cell embryos. These results suggest that mammalian oocytes have more complex mecha- nisms to establish DNA replication competence when compared to their Xenopus counterparts.Fil: Murai, Shin. State University Of Pennsylvania; Estados UnidosFil: Stein, Paula. University of Pennsylvania; Estados UnidosFil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; Argentina. University of Pennsylvania; Estados UnidosFil: Yamashita, Shigeru. Toho University. School of Medicine; JapónFil: Schultz, Richard M.. University of Pennsylvania; Estados UnidosElsevier Inc2010-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14247Murai, Shin; Stein, Paula; Buffone, Mariano Gabriel; Yamashita, Shigeru; Schultz, Richard M.; Recruitment of Orc6l, a dormant maternal mRNA in mouse oocytes, is essential for DNA replication in 1-cell embryos; Elsevier Inc; Developmental Biology; 341; 1; 1-5-2010; 205-2120012-16061095-564Xenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0012160610001351info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ydbio.2010.02.027info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2854205/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:03:48Zoai:ri.conicet.gov.ar:11336/14247instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:03:48.563CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Recruitment of Orc6l, a dormant maternal mRNA in mouse oocytes, is essential for DNA replication in 1-cell embryos
title Recruitment of Orc6l, a dormant maternal mRNA in mouse oocytes, is essential for DNA replication in 1-cell embryos
spellingShingle Recruitment of Orc6l, a dormant maternal mRNA in mouse oocytes, is essential for DNA replication in 1-cell embryos
Murai, Shin
Dna Replication
Dormant Maternal Mrna
Cytoplasmic Poladenylation Element
Mouse 1-Cell Embryo
title_short Recruitment of Orc6l, a dormant maternal mRNA in mouse oocytes, is essential for DNA replication in 1-cell embryos
title_full Recruitment of Orc6l, a dormant maternal mRNA in mouse oocytes, is essential for DNA replication in 1-cell embryos
title_fullStr Recruitment of Orc6l, a dormant maternal mRNA in mouse oocytes, is essential for DNA replication in 1-cell embryos
title_full_unstemmed Recruitment of Orc6l, a dormant maternal mRNA in mouse oocytes, is essential for DNA replication in 1-cell embryos
title_sort Recruitment of Orc6l, a dormant maternal mRNA in mouse oocytes, is essential for DNA replication in 1-cell embryos
dc.creator.none.fl_str_mv Murai, Shin
Stein, Paula
Buffone, Mariano Gabriel
Yamashita, Shigeru
Schultz, Richard M.
author Murai, Shin
author_facet Murai, Shin
Stein, Paula
Buffone, Mariano Gabriel
Yamashita, Shigeru
Schultz, Richard M.
author_role author
author2 Stein, Paula
Buffone, Mariano Gabriel
Yamashita, Shigeru
Schultz, Richard M.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Dna Replication
Dormant Maternal Mrna
Cytoplasmic Poladenylation Element
Mouse 1-Cell Embryo
topic Dna Replication
Dormant Maternal Mrna
Cytoplasmic Poladenylation Element
Mouse 1-Cell Embryo
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Mouse oocytes acquire the ability to replicate DNA during meiotic maturation, presumably to ensure that DNA replication does not occur precociously between MI and MII and only after fertilization. Acquisition of DNA replication competence requires protein synthesis, but the identity of the proteins required for DNA replication is poorly described. In Xenopus, the only component missing for DNA replication competence is CDC6, which is synthesized from a dormant maternal mRNA recruited during oocyte maturation, and a similar situation also occurs during mouse oocyte maturation. We report that ORC6L is another component required for acquisition of DNA replication competence that is absent in mouse oocytes. The dormant maternal Orc6l mRNA is recruited during maturation via a CPE present in its 3␣ UTR. RNAi-mediated ablation of maternal Orc6l mRNA prevents the maturation-associated increase in ORC6L protein and inhibits DNA replication in 1-cell embryos. These results suggest that mammalian oocytes have more complex mecha- nisms to establish DNA replication competence when compared to their Xenopus counterparts.
Fil: Murai, Shin. State University Of Pennsylvania; Estados Unidos
Fil: Stein, Paula. University of Pennsylvania; Estados Unidos
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; Argentina. University of Pennsylvania; Estados Unidos
Fil: Yamashita, Shigeru. Toho University. School of Medicine; Japón
Fil: Schultz, Richard M.. University of Pennsylvania; Estados Unidos
description Mouse oocytes acquire the ability to replicate DNA during meiotic maturation, presumably to ensure that DNA replication does not occur precociously between MI and MII and only after fertilization. Acquisition of DNA replication competence requires protein synthesis, but the identity of the proteins required for DNA replication is poorly described. In Xenopus, the only component missing for DNA replication competence is CDC6, which is synthesized from a dormant maternal mRNA recruited during oocyte maturation, and a similar situation also occurs during mouse oocyte maturation. We report that ORC6L is another component required for acquisition of DNA replication competence that is absent in mouse oocytes. The dormant maternal Orc6l mRNA is recruited during maturation via a CPE present in its 3␣ UTR. RNAi-mediated ablation of maternal Orc6l mRNA prevents the maturation-associated increase in ORC6L protein and inhibits DNA replication in 1-cell embryos. These results suggest that mammalian oocytes have more complex mecha- nisms to establish DNA replication competence when compared to their Xenopus counterparts.
publishDate 2010
dc.date.none.fl_str_mv 2010-05-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14247
Murai, Shin; Stein, Paula; Buffone, Mariano Gabriel; Yamashita, Shigeru; Schultz, Richard M.; Recruitment of Orc6l, a dormant maternal mRNA in mouse oocytes, is essential for DNA replication in 1-cell embryos; Elsevier Inc; Developmental Biology; 341; 1; 1-5-2010; 205-212
0012-1606
1095-564X
url http://hdl.handle.net/11336/14247
identifier_str_mv Murai, Shin; Stein, Paula; Buffone, Mariano Gabriel; Yamashita, Shigeru; Schultz, Richard M.; Recruitment of Orc6l, a dormant maternal mRNA in mouse oocytes, is essential for DNA replication in 1-cell embryos; Elsevier Inc; Developmental Biology; 341; 1; 1-5-2010; 205-212
0012-1606
1095-564X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0012160610001351
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ydbio.2010.02.027
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2854205/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Inc
publisher.none.fl_str_mv Elsevier Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613858517319680
score 13.070432

Publicaciones similares