Electroporation of postimplantation mouse embryos in utero

Autores
Huang, Cheng Chiu; Carcagno, Abel Luis
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Gene transfer by electroporation is possible in mouse fetuses within the uterus. As described in this protocol, the pregnant female is anesthetized, the abdominal cavity is opened, and the uterus with the fetuses is exteriorized. A solution of plasmid DNA is injected through the uterine wall directly into the fetus, typically into a cavity like the brain ventricle, guided by fiber optic illumination. Electrodes are positioned on the uterus around the region of the fetus that was injected, and electrical pulses are delivered. The uterus is returned to the abdominal cavity, the body wall is sutured closed, and the female is allowed to recover. The manipulated fetuses can then be collected and analyzed at various times after the electroporation. This method allows experimental access to later-stage developing mouse embryos.
Fil: Huang, Cheng Chiu. University of Texas MD Anderson Cancer Center; Argentina
Fil: Carcagno, Abel Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Materia
ELECTROPORATION
MOUSE
IN UTERO
EMBRYO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/90524
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Electroporation of postimplantation mouse embryos in uteroHuang, Cheng ChiuCarcagno, Abel LuisELECTROPORATIONMOUSEIN UTEROEMBRYOhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Gene transfer by electroporation is possible in mouse fetuses within the uterus. As described in this protocol, the pregnant female is anesthetized, the abdominal cavity is opened, and the uterus with the fetuses is exteriorized. A solution of plasmid DNA is injected through the uterine wall directly into the fetus, typically into a cavity like the brain ventricle, guided by fiber optic illumination. Electrodes are positioned on the uterus around the region of the fetus that was injected, and electrical pulses are delivered. The uterus is returned to the abdominal cavity, the body wall is sutured closed, and the female is allowed to recover. The manipulated fetuses can then be collected and analyzed at various times after the electroporation. This method allows experimental access to later-stage developing mouse embryos.Fil: Huang, Cheng Chiu. University of Texas MD Anderson Cancer Center; ArgentinaFil: Carcagno, Abel Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaCold Spring Harbor Laboratory Press2018-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/90524Huang, Cheng Chiu; Carcagno, Abel Luis; Electroporation of postimplantation mouse embryos in utero; Cold Spring Harbor Laboratory Press; Cold Spring Harbor Protocols; 2018; 2; 2-2018; 99-1021940-3402CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.cshprotocols.org/lookup/doi/10.1101/pdb.prot094060info:eu-repo/semantics/altIdentifier/doi/10.1101/pdb.prot094060info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:11Zoai:ri.conicet.gov.ar:11336/90524instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:12.181CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Electroporation of postimplantation mouse embryos in utero
title Electroporation of postimplantation mouse embryos in utero
spellingShingle Electroporation of postimplantation mouse embryos in utero
Huang, Cheng Chiu
ELECTROPORATION
MOUSE
IN UTERO
EMBRYO
title_short Electroporation of postimplantation mouse embryos in utero
title_full Electroporation of postimplantation mouse embryos in utero
title_fullStr Electroporation of postimplantation mouse embryos in utero
title_full_unstemmed Electroporation of postimplantation mouse embryos in utero
title_sort Electroporation of postimplantation mouse embryos in utero
dc.creator.none.fl_str_mv Huang, Cheng Chiu
Carcagno, Abel Luis
author Huang, Cheng Chiu
author_facet Huang, Cheng Chiu
Carcagno, Abel Luis
author_role author
author2 Carcagno, Abel Luis
author2_role author
dc.subject.none.fl_str_mv ELECTROPORATION
MOUSE
IN UTERO
EMBRYO
topic ELECTROPORATION
MOUSE
IN UTERO
EMBRYO
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Gene transfer by electroporation is possible in mouse fetuses within the uterus. As described in this protocol, the pregnant female is anesthetized, the abdominal cavity is opened, and the uterus with the fetuses is exteriorized. A solution of plasmid DNA is injected through the uterine wall directly into the fetus, typically into a cavity like the brain ventricle, guided by fiber optic illumination. Electrodes are positioned on the uterus around the region of the fetus that was injected, and electrical pulses are delivered. The uterus is returned to the abdominal cavity, the body wall is sutured closed, and the female is allowed to recover. The manipulated fetuses can then be collected and analyzed at various times after the electroporation. This method allows experimental access to later-stage developing mouse embryos.
Fil: Huang, Cheng Chiu. University of Texas MD Anderson Cancer Center; Argentina
Fil: Carcagno, Abel Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
description Gene transfer by electroporation is possible in mouse fetuses within the uterus. As described in this protocol, the pregnant female is anesthetized, the abdominal cavity is opened, and the uterus with the fetuses is exteriorized. A solution of plasmid DNA is injected through the uterine wall directly into the fetus, typically into a cavity like the brain ventricle, guided by fiber optic illumination. Electrodes are positioned on the uterus around the region of the fetus that was injected, and electrical pulses are delivered. The uterus is returned to the abdominal cavity, the body wall is sutured closed, and the female is allowed to recover. The manipulated fetuses can then be collected and analyzed at various times after the electroporation. This method allows experimental access to later-stage developing mouse embryos.
publishDate 2018
dc.date.none.fl_str_mv 2018-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/90524
Huang, Cheng Chiu; Carcagno, Abel Luis; Electroporation of postimplantation mouse embryos in utero; Cold Spring Harbor Laboratory Press; Cold Spring Harbor Protocols; 2018; 2; 2-2018; 99-102
1940-3402
CONICET Digital
CONICET
url http://hdl.handle.net/11336/90524
identifier_str_mv Huang, Cheng Chiu; Carcagno, Abel Luis; Electroporation of postimplantation mouse embryos in utero; Cold Spring Harbor Laboratory Press; Cold Spring Harbor Protocols; 2018; 2; 2-2018; 99-102
1940-3402
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.cshprotocols.org/lookup/doi/10.1101/pdb.prot094060
info:eu-repo/semantics/altIdentifier/doi/10.1101/pdb.prot094060
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268959099846656
score 13.13397

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