Effect of number of oocytes and embryos on in vitro oocyte maturation, fertilization and embryo development in bovine

• Autores: Salvador, I.; Cebrian-Serrano, A.; Salamone, Daniel Felipe; Silvestre, M. A.
• Año de publicación: 2011
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Spanish Journal of Agricultural Research; Vol.9, no.3; 744-752; http://www.inia.es/
• Materia: LOW EMBRYO CULTURE; SINGLE; BOVINAE
• Institución: Universidad de Buenos Aires. Facultad de Agronomía

Fil: Salvador, I. Centro de Tecnología Animal. Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA). Apdo. 187, 12400 Segorbe (Castellón), Spain.
Fil: Cebrian-Serrano, A. Centro de Tecnología Animal. Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA). Apdo. 187, 12400 Segorbe (Castellón), Spain.
Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía. Laboratorio de Biotecnología Animal. Buenos Aires, Argentina.
Fil: Silvestre, M. A. Centro de Tecnología Animal. Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA). Apdo. 187, 12400 Segorbe (Castellón), Spain.
The aim of this study was to identify the in vitro development stage at which the culture of a single or low number (n = 5 or 10) of oocytes/embryos could impair development in comparison with culture in group (n = 50). In the Experiment 1, it was confirmed that single in vitro embryo production yielded lower cleavage and blastocyst rates than in group (49.4 vs. 83.0 percent; 0 percent vs. 37.8 percent, respectively; p less than 0.05). In Experiment 2 and 3, it was observed no effect on embryo development of culturing single or low number of oocytes during maturation and fertilization, respectively. In Experiment 4, it was observed a detrimental effect on blastocyst rate when cultured single or low number of embryos during post-fertilization in vitro culture (2.9; 10.2-10.8; 33.2 percent in single, low number of embryos (5-10), and controlgrouped, respectively; p less than 0.05). In Experiment 5, it was observed that the last part of the culture period (day 3 onwards) seemed to be more affected by the low number of embryos placed in culture. In conclusion, post-fertilization culture, especially on days 3 to 7 after fertilization, seems to be the most important stage for embryo development on single and/or low number (5-10) of embryos culture.