Peumus boldus Extract Inhibits Lipid Accumulation in 3T3-L1 Adipocytes

Autores
Montaldo, Laura Andrea; Bendezu Meza, Llerson; de Marzi, Mauricio Cesar; Guerra, Liliana Noemi
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Obesity is a metabolic condition of epidemic scale. Previously, we showedthat antioxidant extracts from Ribes nigrum had antioxidant and anti-adipogenic effectsin mature adipocytes (AD). Here, we evaluated an aqueous extract from Peumus boldus(Boldo) in AD and studied its effect on reactive oxygen species (ROS) and lipid production.We analyzed the antioxidant activity (AA) of the Boldo extract using the DPPH techniqueand polyphenol (Pph) content via Folin’s reagent. In AD, we evaluated ROS production,catalase (CAT) activity, intracellular triglyceride (Tg) and cholesterol (Chol) contents, nitricoxide (NO) production via Griess reagent, and the levels of glycerol (Gly) and TNF-αreleased in the culture medium. We showed that the Boldo extract has high AA. In vitro,Boldo treatment decreased ROS intracellular production and CAT activity. In addition, theBoldo extract was effective in reducing Tg and Chol levels and NO production. We didnot identify significant differences in Gly released or TNF-α secreted. We suggest that theBoldo extract has antioxidant and anti-adipogenic effects, but we did not observe lipolyticeffects. Boldo did not modify inflammatory markers.
Fil: Montaldo, Laura Andrea. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
Fil: Bendezu Meza, Llerson. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
Fil: de Marzi, Mauricio Cesar. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina
Fil: Guerra, Liliana Noemi. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina
Materia
antioxidants
polyphenols
oxidative stress
adipocytes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/276541
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Peumus boldus Extract Inhibits Lipid Accumulation in 3T3-L1 AdipocytesMontaldo, Laura AndreaBendezu Meza, Llersonde Marzi, Mauricio CesarGuerra, Liliana Noemiantioxidantspolyphenolsoxidative stressadipocyteshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Obesity is a metabolic condition of epidemic scale. Previously, we showedthat antioxidant extracts from Ribes nigrum had antioxidant and anti-adipogenic effectsin mature adipocytes (AD). Here, we evaluated an aqueous extract from Peumus boldus(Boldo) in AD and studied its effect on reactive oxygen species (ROS) and lipid production.We analyzed the antioxidant activity (AA) of the Boldo extract using the DPPH techniqueand polyphenol (Pph) content via Folin’s reagent. In AD, we evaluated ROS production,catalase (CAT) activity, intracellular triglyceride (Tg) and cholesterol (Chol) contents, nitricoxide (NO) production via Griess reagent, and the levels of glycerol (Gly) and TNF-αreleased in the culture medium. We showed that the Boldo extract has high AA. In vitro,Boldo treatment decreased ROS intracellular production and CAT activity. In addition, theBoldo extract was effective in reducing Tg and Chol levels and NO production. We didnot identify significant differences in Gly released or TNF-α secreted. We suggest that theBoldo extract has antioxidant and anti-adipogenic effects, but we did not observe lipolyticeffects. Boldo did not modify inflammatory markers.Fil: Montaldo, Laura Andrea. Universidad Nacional de Luján. Departamento de Ciencias Básicas; ArgentinaFil: Bendezu Meza, Llerson. Universidad Nacional de Luján. Departamento de Ciencias Básicas; ArgentinaFil: de Marzi, Mauricio Cesar. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; ArgentinaFil: Guerra, Liliana Noemi. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; ArgentinaMolecular Diversity Preservation International2025-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/276541Montaldo, Laura Andrea; Bendezu Meza, Llerson; de Marzi, Mauricio Cesar; Guerra, Liliana Noemi; Peumus boldus Extract Inhibits Lipid Accumulation in 3T3-L1 Adipocytes; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 26; 9; 5-2025; 1-161422-0067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/26/9/4326info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms26094326info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-17T15:07:58Zoai:ri.conicet.gov.ar:11336/276541instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-17 15:07:58.99CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Peumus boldus Extract Inhibits Lipid Accumulation in 3T3-L1 Adipocytes
title Peumus boldus Extract Inhibits Lipid Accumulation in 3T3-L1 Adipocytes
spellingShingle Peumus boldus Extract Inhibits Lipid Accumulation in 3T3-L1 Adipocytes
Montaldo, Laura Andrea
antioxidants
polyphenols
oxidative stress
adipocytes
title_short Peumus boldus Extract Inhibits Lipid Accumulation in 3T3-L1 Adipocytes
title_full Peumus boldus Extract Inhibits Lipid Accumulation in 3T3-L1 Adipocytes
title_fullStr Peumus boldus Extract Inhibits Lipid Accumulation in 3T3-L1 Adipocytes
title_full_unstemmed Peumus boldus Extract Inhibits Lipid Accumulation in 3T3-L1 Adipocytes
title_sort Peumus boldus Extract Inhibits Lipid Accumulation in 3T3-L1 Adipocytes
dc.creator.none.fl_str_mv Montaldo, Laura Andrea
Bendezu Meza, Llerson
de Marzi, Mauricio Cesar
Guerra, Liliana Noemi
author Montaldo, Laura Andrea
author_facet Montaldo, Laura Andrea
Bendezu Meza, Llerson
de Marzi, Mauricio Cesar
Guerra, Liliana Noemi
author_role author
author2 Bendezu Meza, Llerson
de Marzi, Mauricio Cesar
Guerra, Liliana Noemi
author2_role author
author
author
dc.subject.none.fl_str_mv antioxidants
polyphenols
oxidative stress
adipocytes
topic antioxidants
polyphenols
oxidative stress
adipocytes
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Obesity is a metabolic condition of epidemic scale. Previously, we showedthat antioxidant extracts from Ribes nigrum had antioxidant and anti-adipogenic effectsin mature adipocytes (AD). Here, we evaluated an aqueous extract from Peumus boldus(Boldo) in AD and studied its effect on reactive oxygen species (ROS) and lipid production.We analyzed the antioxidant activity (AA) of the Boldo extract using the DPPH techniqueand polyphenol (Pph) content via Folin’s reagent. In AD, we evaluated ROS production,catalase (CAT) activity, intracellular triglyceride (Tg) and cholesterol (Chol) contents, nitricoxide (NO) production via Griess reagent, and the levels of glycerol (Gly) and TNF-αreleased in the culture medium. We showed that the Boldo extract has high AA. In vitro,Boldo treatment decreased ROS intracellular production and CAT activity. In addition, theBoldo extract was effective in reducing Tg and Chol levels and NO production. We didnot identify significant differences in Gly released or TNF-α secreted. We suggest that theBoldo extract has antioxidant and anti-adipogenic effects, but we did not observe lipolyticeffects. Boldo did not modify inflammatory markers.
Fil: Montaldo, Laura Andrea. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
Fil: Bendezu Meza, Llerson. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
Fil: de Marzi, Mauricio Cesar. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina
Fil: Guerra, Liliana Noemi. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina
description Obesity is a metabolic condition of epidemic scale. Previously, we showedthat antioxidant extracts from Ribes nigrum had antioxidant and anti-adipogenic effectsin mature adipocytes (AD). Here, we evaluated an aqueous extract from Peumus boldus(Boldo) in AD and studied its effect on reactive oxygen species (ROS) and lipid production.We analyzed the antioxidant activity (AA) of the Boldo extract using the DPPH techniqueand polyphenol (Pph) content via Folin’s reagent. In AD, we evaluated ROS production,catalase (CAT) activity, intracellular triglyceride (Tg) and cholesterol (Chol) contents, nitricoxide (NO) production via Griess reagent, and the levels of glycerol (Gly) and TNF-αreleased in the culture medium. We showed that the Boldo extract has high AA. In vitro,Boldo treatment decreased ROS intracellular production and CAT activity. In addition, theBoldo extract was effective in reducing Tg and Chol levels and NO production. We didnot identify significant differences in Gly released or TNF-α secreted. We suggest that theBoldo extract has antioxidant and anti-adipogenic effects, but we did not observe lipolyticeffects. Boldo did not modify inflammatory markers.
publishDate 2025
dc.date.none.fl_str_mv 2025-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/276541
Montaldo, Laura Andrea; Bendezu Meza, Llerson; de Marzi, Mauricio Cesar; Guerra, Liliana Noemi; Peumus boldus Extract Inhibits Lipid Accumulation in 3T3-L1 Adipocytes; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 26; 9; 5-2025; 1-16
1422-0067
CONICET Digital
CONICET
url http://hdl.handle.net/11336/276541
identifier_str_mv Montaldo, Laura Andrea; Bendezu Meza, Llerson; de Marzi, Mauricio Cesar; Guerra, Liliana Noemi; Peumus boldus Extract Inhibits Lipid Accumulation in 3T3-L1 Adipocytes; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 26; 9; 5-2025; 1-16
1422-0067
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/26/9/4326
info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms26094326
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.176297

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