Menadione and iron overload trigger neutral lipid remodelling in adipocytes and adipose tissue

Autores
Funk, Melania Iara; Maniscalchi, Athina; Benzi Juncos, Oriana Nicole; Alza, Natalia Paola; Conde, Melisa Ailén; Salvador, Gabriela Alejandra; Uranga, Romina Maria
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
A growing body of evidence indicates that oxidative stress (OS) can cause an increase in preadipocyte proliferation, and adipocyte differentiation. However, the biochemical mechanisms by which OS alters adipocyte neutral lipid metabolism are not completely elucidated. Menadione and iron (Fe) overload are well known OS enhancers through the induction of Fenton and Haber-Weiss reactions. We have previously demonstrated that menadione exposure (20 and 50 µM) in differentiated 3T3- L1 adipocytes results in increased cell oxidant levels and downregulation of adipogenic genes and transcription factors. In this work, our goal was to investigate the effect of menadione and Fe overload in neutral lipid metabolism both in adipocyte cell culture and by using an in vivo model. For this purpose, we worked with differentiated adipocytes challenged with menadione and C57BL/6 mice exposed to Fe overload. Differentiated 3T3-L1 adipocytes were exposed to menadione for 24 h, and then neutral lipid profile was analyzed. For the in vivo OS model, Fe-saccharate (800 or 1332 mg/kg) or vehicle were administered by intraperitoneal injection (four-dose scheme in 16 days). Plasma, visceral and gonadal adipose tissue obtained from Fetreated animals and controls were used to determine OS markers and neutral lipids. In mice, lipid peroxide levels and conjugated dienes derived from fatty acid oxidation were increased in visceral and gonadal adipose tissue after Fe treatment. No changes were detected in catalase activity. Moreover, OS markers from plasma were also augmented by Fe-overload. We found that menadione-induced OS increased triglyceride content and activated lipolysis in adipocytes. Intriguingly, monoacylglycerol and diacylglycerol levels were decreased, and no difference was observed in triacylglycerol levels in Feoverloaded mice. Fe treatment caused a diminution in wet weight of both gonadal and visceral adipose tissues when compared to control animals. Consequently, the ratio triacylglycerol/g wet tissue was increased by the effect of OS. Together, our results demonstrate that different OS inducers promote an active remodeling of neutral lipids in adipose tissue.
Fil: Funk, Melania Iara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Maniscalchi, Athina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Benzi Juncos, Oriana Nicole. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Alza, Natalia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Química; Argentina
Fil: Conde, Melisa Ailén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Uranga, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research (SAIB), XVI Annual Meeting of the Argentinean Society for General Microbiology (SAMIGE)
Mendoza
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Sociedad Argentina de Microbiología General
Materia
Oxidative stress
lipid remodeling
adipocyte
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195359
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/195359
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network_name_str CONICET Digital (CONICET)
spelling Menadione and iron overload trigger neutral lipid remodelling in adipocytes and adipose tissueFunk, Melania IaraManiscalchi, AthinaBenzi Juncos, Oriana NicoleAlza, Natalia PaolaConde, Melisa AilénSalvador, Gabriela AlejandraUranga, Romina MariaOxidative stresslipid remodelingadipocytehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A growing body of evidence indicates that oxidative stress (OS) can cause an increase in preadipocyte proliferation, and adipocyte differentiation. However, the biochemical mechanisms by which OS alters adipocyte neutral lipid metabolism are not completely elucidated. Menadione and iron (Fe) overload are well known OS enhancers through the induction of Fenton and Haber-Weiss reactions. We have previously demonstrated that menadione exposure (20 and 50 µM) in differentiated 3T3- L1 adipocytes results in increased cell oxidant levels and downregulation of adipogenic genes and transcription factors. In this work, our goal was to investigate the effect of menadione and Fe overload in neutral lipid metabolism both in adipocyte cell culture and by using an in vivo model. For this purpose, we worked with differentiated adipocytes challenged with menadione and C57BL/6 mice exposed to Fe overload. Differentiated 3T3-L1 adipocytes were exposed to menadione for 24 h, and then neutral lipid profile was analyzed. For the in vivo OS model, Fe-saccharate (800 or 1332 mg/kg) or vehicle were administered by intraperitoneal injection (four-dose scheme in 16 days). Plasma, visceral and gonadal adipose tissue obtained from Fetreated animals and controls were used to determine OS markers and neutral lipids. In mice, lipid peroxide levels and conjugated dienes derived from fatty acid oxidation were increased in visceral and gonadal adipose tissue after Fe treatment. No changes were detected in catalase activity. Moreover, OS markers from plasma were also augmented by Fe-overload. We found that menadione-induced OS increased triglyceride content and activated lipolysis in adipocytes. Intriguingly, monoacylglycerol and diacylglycerol levels were decreased, and no difference was observed in triacylglycerol levels in Feoverloaded mice. Fe treatment caused a diminution in wet weight of both gonadal and visceral adipose tissues when compared to control animals. Consequently, the ratio triacylglycerol/g wet tissue was increased by the effect of OS. Together, our results demonstrate that different OS inducers promote an active remodeling of neutral lipids in adipose tissue.Fil: Funk, Melania Iara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Maniscalchi, Athina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Benzi Juncos, Oriana Nicole. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Alza, Natalia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Química; ArgentinaFil: Conde, Melisa Ailén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Uranga, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaLVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research (SAIB), XVI Annual Meeting of the Argentinean Society for General Microbiology (SAMIGE)MendozaArgentinaSociedad Argentina de Investigación Bioquímica y Biología MolecularSociedad Argentina de Microbiología GeneralTech Science Press2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195359Menadione and iron overload trigger neutral lipid remodelling in adipocytes and adipose tissue; LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research (SAIB), XVI Annual Meeting of the Argentinean Society for General Microbiology (SAMIGE); Mendoza; Argentina; 2021; 87-870327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://techscience.com/biocell/v46nSuppl.1/46213Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:48Zoai:ri.conicet.gov.ar:11336/195359instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:49.089CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Menadione and iron overload trigger neutral lipid remodelling in adipocytes and adipose tissue
title Menadione and iron overload trigger neutral lipid remodelling in adipocytes and adipose tissue
spellingShingle Menadione and iron overload trigger neutral lipid remodelling in adipocytes and adipose tissue
Funk, Melania Iara
Oxidative stress
lipid remodeling
adipocyte
title_short Menadione and iron overload trigger neutral lipid remodelling in adipocytes and adipose tissue
title_full Menadione and iron overload trigger neutral lipid remodelling in adipocytes and adipose tissue
title_fullStr Menadione and iron overload trigger neutral lipid remodelling in adipocytes and adipose tissue
title_full_unstemmed Menadione and iron overload trigger neutral lipid remodelling in adipocytes and adipose tissue
title_sort Menadione and iron overload trigger neutral lipid remodelling in adipocytes and adipose tissue
dc.creator.none.fl_str_mv Funk, Melania Iara
Maniscalchi, Athina
Benzi Juncos, Oriana Nicole
Alza, Natalia Paola
Conde, Melisa Ailén
Salvador, Gabriela Alejandra
Uranga, Romina Maria
author Funk, Melania Iara
author_facet Funk, Melania Iara
Maniscalchi, Athina
Benzi Juncos, Oriana Nicole
Alza, Natalia Paola
Conde, Melisa Ailén
Salvador, Gabriela Alejandra
Uranga, Romina Maria
author_role author
author2 Maniscalchi, Athina
Benzi Juncos, Oriana Nicole
Alza, Natalia Paola
Conde, Melisa Ailén
Salvador, Gabriela Alejandra
Uranga, Romina Maria
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Oxidative stress
lipid remodeling
adipocyte
topic Oxidative stress
lipid remodeling
adipocyte
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A growing body of evidence indicates that oxidative stress (OS) can cause an increase in preadipocyte proliferation, and adipocyte differentiation. However, the biochemical mechanisms by which OS alters adipocyte neutral lipid metabolism are not completely elucidated. Menadione and iron (Fe) overload are well known OS enhancers through the induction of Fenton and Haber-Weiss reactions. We have previously demonstrated that menadione exposure (20 and 50 µM) in differentiated 3T3- L1 adipocytes results in increased cell oxidant levels and downregulation of adipogenic genes and transcription factors. In this work, our goal was to investigate the effect of menadione and Fe overload in neutral lipid metabolism both in adipocyte cell culture and by using an in vivo model. For this purpose, we worked with differentiated adipocytes challenged with menadione and C57BL/6 mice exposed to Fe overload. Differentiated 3T3-L1 adipocytes were exposed to menadione for 24 h, and then neutral lipid profile was analyzed. For the in vivo OS model, Fe-saccharate (800 or 1332 mg/kg) or vehicle were administered by intraperitoneal injection (four-dose scheme in 16 days). Plasma, visceral and gonadal adipose tissue obtained from Fetreated animals and controls were used to determine OS markers and neutral lipids. In mice, lipid peroxide levels and conjugated dienes derived from fatty acid oxidation were increased in visceral and gonadal adipose tissue after Fe treatment. No changes were detected in catalase activity. Moreover, OS markers from plasma were also augmented by Fe-overload. We found that menadione-induced OS increased triglyceride content and activated lipolysis in adipocytes. Intriguingly, monoacylglycerol and diacylglycerol levels were decreased, and no difference was observed in triacylglycerol levels in Feoverloaded mice. Fe treatment caused a diminution in wet weight of both gonadal and visceral adipose tissues when compared to control animals. Consequently, the ratio triacylglycerol/g wet tissue was increased by the effect of OS. Together, our results demonstrate that different OS inducers promote an active remodeling of neutral lipids in adipose tissue.
Fil: Funk, Melania Iara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Maniscalchi, Athina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Benzi Juncos, Oriana Nicole. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Alza, Natalia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Química; Argentina
Fil: Conde, Melisa Ailén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Uranga, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research (SAIB), XVI Annual Meeting of the Argentinean Society for General Microbiology (SAMIGE)
Mendoza
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Sociedad Argentina de Microbiología General
description A growing body of evidence indicates that oxidative stress (OS) can cause an increase in preadipocyte proliferation, and adipocyte differentiation. However, the biochemical mechanisms by which OS alters adipocyte neutral lipid metabolism are not completely elucidated. Menadione and iron (Fe) overload are well known OS enhancers through the induction of Fenton and Haber-Weiss reactions. We have previously demonstrated that menadione exposure (20 and 50 µM) in differentiated 3T3- L1 adipocytes results in increased cell oxidant levels and downregulation of adipogenic genes and transcription factors. In this work, our goal was to investigate the effect of menadione and Fe overload in neutral lipid metabolism both in adipocyte cell culture and by using an in vivo model. For this purpose, we worked with differentiated adipocytes challenged with menadione and C57BL/6 mice exposed to Fe overload. Differentiated 3T3-L1 adipocytes were exposed to menadione for 24 h, and then neutral lipid profile was analyzed. For the in vivo OS model, Fe-saccharate (800 or 1332 mg/kg) or vehicle were administered by intraperitoneal injection (four-dose scheme in 16 days). Plasma, visceral and gonadal adipose tissue obtained from Fetreated animals and controls were used to determine OS markers and neutral lipids. In mice, lipid peroxide levels and conjugated dienes derived from fatty acid oxidation were increased in visceral and gonadal adipose tissue after Fe treatment. No changes were detected in catalase activity. Moreover, OS markers from plasma were also augmented by Fe-overload. We found that menadione-induced OS increased triglyceride content and activated lipolysis in adipocytes. Intriguingly, monoacylglycerol and diacylglycerol levels were decreased, and no difference was observed in triacylglycerol levels in Feoverloaded mice. Fe treatment caused a diminution in wet weight of both gonadal and visceral adipose tissues when compared to control animals. Consequently, the ratio triacylglycerol/g wet tissue was increased by the effect of OS. Together, our results demonstrate that different OS inducers promote an active remodeling of neutral lipids in adipose tissue.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195359
Menadione and iron overload trigger neutral lipid remodelling in adipocytes and adipose tissue; LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research (SAIB), XVI Annual Meeting of the Argentinean Society for General Microbiology (SAMIGE); Mendoza; Argentina; 2021; 87-87
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195359
identifier_str_mv Menadione and iron overload trigger neutral lipid remodelling in adipocytes and adipose tissue; LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research (SAIB), XVI Annual Meeting of the Argentinean Society for General Microbiology (SAMIGE); Mendoza; Argentina; 2021; 87-87
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://techscience.com/biocell/v46nSuppl.1/46213
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rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
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