Effect of oxidative stress on adipocyte differentiation

Autores
Blazquez, Ana Catalina; Piwien Pilipuk, Graciela; Salvador, Gabriela Alejandra; Uranga, Romina Maria
Año de publicación
2017
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Oxidative stress (OS) is a major characteristic of adipose tissue in obese patients and its role in adipose hyperplasia/hypertrophy has not been elucidated. The aim of our study was to analyze the effect of OS during adipogenesis as well on differentiated adipocytes. For this purpose, we worked with preadipocytes 3T3-L1 and menadione, a synthetic form of vitamin K known to generate intracellular oxygen species. Two different models were used: the “chronic”, in which OS (5, 10 y 15 μM menadione) was present during the whole process of 3T3-L1 differentiation, and the “acute”, in which differentiated adipo- cytes were exposed to OS (20 y 50 μM menadione) for 5 h. Adipogenesis, evaluated by Oil Red O staining as well as by the expression of adipogenic markers (PPARγ, C/EBPα, FAS, FABP4) by Western blot, was observed to be decreased in the chronic mod- el in a menadione concentration-dependent manner (p<0.01). The adipogenic markers were also found to significantly decrease upon acute treatment (p<0.001). However, no significant morphological changes were observed in differentiated adipocytes acutely treated with menadione. PI3K/Akt and ERK1/2 showed to be inversely reg- ulated: Akt was found to be inactivated whereas ERK1/2 were found to be activated in both models of menadione-induced OS. Interest- ingly, experiments in which adipogenesis was evaluated in the pres- ence of LY294002, a pharmacological PI3K inhibitor, but in the ab- sence of menadione showed a significant decreased differentiation. Our results demonstrate that acute menadione-induced OS triggers a gene expression program leading to the decreased expression of transcription factors and enzymes which are crucial for lipogenesis and, on the other hand, chronic menadione-induced OS prevents adipogenesis, presumably in part, in a PI3K-dependent manner. Keywords: adipogenesis, oxidative stress, lipid metabolism.
Fil: Blazquez, Ana Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Piwien Pilipuk, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Uranga, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XIX Reunión Anual De La Sociedad Argentina De Biología; XlVI Reunión Anual De La Sociedad Argentina De Biofísica; Reunión De La Sociedad Argentina De Andrología; LXII Reunión Anual De La Sociedad Argentina De Investigación Clínica; LXV Reunión Anual De La Sociedad Argentina De Inmunología; XlX Reunión Anual De La Sociedad Argentina De Farmacología Experimental; LIII Reunión Anual De La Sociedad Argentina De Investigación Bioquímica y Biología Molecular; Reunión Anual De La Sociedad Argentina De Fisiología y XXIX Reunión Anual De La Sociedad Argentina De Protozoología
Buenos Aires
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Sociedad Argentina de Inmunología
Sociedad Argentina de Andrología
Sociedad Argentina de Biofísica
Sociedad Argentina de Biología
Sociedad Argentina de Farmacología Experimental
Sociedad Argentina de Fisiologia
Sociedad Argentina de Hematología
Sociedad Argentina de Protozoología
Materia
ADIPOCYTE
OXIDATIVE STRESS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/232905
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Effect of oxidative stress on adipocyte differentiationBlazquez, Ana CatalinaPiwien Pilipuk, GracielaSalvador, Gabriela AlejandraUranga, Romina MariaADIPOCYTEOXIDATIVE STRESShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Oxidative stress (OS) is a major characteristic of adipose tissue in obese patients and its role in adipose hyperplasia/hypertrophy has not been elucidated. The aim of our study was to analyze the effect of OS during adipogenesis as well on differentiated adipocytes. For this purpose, we worked with preadipocytes 3T3-L1 and menadione, a synthetic form of vitamin K known to generate intracellular oxygen species. Two different models were used: the “chronic”, in which OS (5, 10 y 15 μM menadione) was present during the whole process of 3T3-L1 differentiation, and the “acute”, in which differentiated adipo- cytes were exposed to OS (20 y 50 μM menadione) for 5 h. Adipogenesis, evaluated by Oil Red O staining as well as by the expression of adipogenic markers (PPARγ, C/EBPα, FAS, FABP4) by Western blot, was observed to be decreased in the chronic mod- el in a menadione concentration-dependent manner (p<0.01). The adipogenic markers were also found to significantly decrease upon acute treatment (p<0.001). However, no significant morphological changes were observed in differentiated adipocytes acutely treated with menadione. PI3K/Akt and ERK1/2 showed to be inversely reg- ulated: Akt was found to be inactivated whereas ERK1/2 were found to be activated in both models of menadione-induced OS. Interest- ingly, experiments in which adipogenesis was evaluated in the pres- ence of LY294002, a pharmacological PI3K inhibitor, but in the ab- sence of menadione showed a significant decreased differentiation. Our results demonstrate that acute menadione-induced OS triggers a gene expression program leading to the decreased expression of transcription factors and enzymes which are crucial for lipogenesis and, on the other hand, chronic menadione-induced OS prevents adipogenesis, presumably in part, in a PI3K-dependent manner. Keywords: adipogenesis, oxidative stress, lipid metabolism.Fil: Blazquez, Ana Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Piwien Pilipuk, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Uranga, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaXIX Reunión Anual De La Sociedad Argentina De Biología; XlVI Reunión Anual De La Sociedad Argentina De Biofísica; Reunión De La Sociedad Argentina De Andrología; LXII Reunión Anual De La Sociedad Argentina De Investigación Clínica; LXV Reunión Anual De La Sociedad Argentina De Inmunología; XlX Reunión Anual De La Sociedad Argentina De Farmacología Experimental; LIII Reunión Anual De La Sociedad Argentina De Investigación Bioquímica y Biología Molecular; Reunión Anual De La Sociedad Argentina De Fisiología y XXIX Reunión Anual De La Sociedad Argentina De ProtozoologíaBuenos AiresArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de Investigación Bioquímica y Biología MolecularSociedad Argentina de InmunologíaSociedad Argentina de AndrologíaSociedad Argentina de BiofísicaSociedad Argentina de BiologíaSociedad Argentina de Farmacología ExperimentalSociedad Argentina de FisiologiaSociedad Argentina de HematologíaSociedad Argentina de ProtozoologíaFundación Revista Medicina2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/232905Effect of oxidative stress on adipocyte differentiation; XIX Reunión Anual De La Sociedad Argentina De Biología; XlVI Reunión Anual De La Sociedad Argentina De Biofísica; Reunión De La Sociedad Argentina De Andrología; LXII Reunión Anual De La Sociedad Argentina De Investigación Clínica; LXV Reunión Anual De La Sociedad Argentina De Inmunología; XlX Reunión Anual De La Sociedad Argentina De Farmacología Experimental; LIII Reunión Anual De La Sociedad Argentina De Investigación Bioquímica y Biología Molecular; Reunión Anual De La Sociedad Argentina De Fisiología y XXIX Reunión Anual De La Sociedad Argentina De Protozoología; Buenos Aires; Argentina; 2017; 397-3970025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicinaNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:54Zoai:ri.conicet.gov.ar:11336/232905instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:54.45CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of oxidative stress on adipocyte differentiation
title Effect of oxidative stress on adipocyte differentiation
spellingShingle Effect of oxidative stress on adipocyte differentiation
Blazquez, Ana Catalina
ADIPOCYTE
OXIDATIVE STRESS
title_short Effect of oxidative stress on adipocyte differentiation
title_full Effect of oxidative stress on adipocyte differentiation
title_fullStr Effect of oxidative stress on adipocyte differentiation
title_full_unstemmed Effect of oxidative stress on adipocyte differentiation
title_sort Effect of oxidative stress on adipocyte differentiation
dc.creator.none.fl_str_mv Blazquez, Ana Catalina
Piwien Pilipuk, Graciela
Salvador, Gabriela Alejandra
Uranga, Romina Maria
author Blazquez, Ana Catalina
author_facet Blazquez, Ana Catalina
Piwien Pilipuk, Graciela
Salvador, Gabriela Alejandra
Uranga, Romina Maria
author_role author
author2 Piwien Pilipuk, Graciela
Salvador, Gabriela Alejandra
Uranga, Romina Maria
author2_role author
author
author
dc.subject.none.fl_str_mv ADIPOCYTE
OXIDATIVE STRESS
topic ADIPOCYTE
OXIDATIVE STRESS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Oxidative stress (OS) is a major characteristic of adipose tissue in obese patients and its role in adipose hyperplasia/hypertrophy has not been elucidated. The aim of our study was to analyze the effect of OS during adipogenesis as well on differentiated adipocytes. For this purpose, we worked with preadipocytes 3T3-L1 and menadione, a synthetic form of vitamin K known to generate intracellular oxygen species. Two different models were used: the “chronic”, in which OS (5, 10 y 15 μM menadione) was present during the whole process of 3T3-L1 differentiation, and the “acute”, in which differentiated adipo- cytes were exposed to OS (20 y 50 μM menadione) for 5 h. Adipogenesis, evaluated by Oil Red O staining as well as by the expression of adipogenic markers (PPARγ, C/EBPα, FAS, FABP4) by Western blot, was observed to be decreased in the chronic mod- el in a menadione concentration-dependent manner (p<0.01). The adipogenic markers were also found to significantly decrease upon acute treatment (p<0.001). However, no significant morphological changes were observed in differentiated adipocytes acutely treated with menadione. PI3K/Akt and ERK1/2 showed to be inversely reg- ulated: Akt was found to be inactivated whereas ERK1/2 were found to be activated in both models of menadione-induced OS. Interest- ingly, experiments in which adipogenesis was evaluated in the pres- ence of LY294002, a pharmacological PI3K inhibitor, but in the ab- sence of menadione showed a significant decreased differentiation. Our results demonstrate that acute menadione-induced OS triggers a gene expression program leading to the decreased expression of transcription factors and enzymes which are crucial for lipogenesis and, on the other hand, chronic menadione-induced OS prevents adipogenesis, presumably in part, in a PI3K-dependent manner. Keywords: adipogenesis, oxidative stress, lipid metabolism.
Fil: Blazquez, Ana Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Piwien Pilipuk, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Uranga, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XIX Reunión Anual De La Sociedad Argentina De Biología; XlVI Reunión Anual De La Sociedad Argentina De Biofísica; Reunión De La Sociedad Argentina De Andrología; LXII Reunión Anual De La Sociedad Argentina De Investigación Clínica; LXV Reunión Anual De La Sociedad Argentina De Inmunología; XlX Reunión Anual De La Sociedad Argentina De Farmacología Experimental; LIII Reunión Anual De La Sociedad Argentina De Investigación Bioquímica y Biología Molecular; Reunión Anual De La Sociedad Argentina De Fisiología y XXIX Reunión Anual De La Sociedad Argentina De Protozoología
Buenos Aires
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Sociedad Argentina de Inmunología
Sociedad Argentina de Andrología
Sociedad Argentina de Biofísica
Sociedad Argentina de Biología
Sociedad Argentina de Farmacología Experimental
Sociedad Argentina de Fisiologia
Sociedad Argentina de Hematología
Sociedad Argentina de Protozoología
description Oxidative stress (OS) is a major characteristic of adipose tissue in obese patients and its role in adipose hyperplasia/hypertrophy has not been elucidated. The aim of our study was to analyze the effect of OS during adipogenesis as well on differentiated adipocytes. For this purpose, we worked with preadipocytes 3T3-L1 and menadione, a synthetic form of vitamin K known to generate intracellular oxygen species. Two different models were used: the “chronic”, in which OS (5, 10 y 15 μM menadione) was present during the whole process of 3T3-L1 differentiation, and the “acute”, in which differentiated adipo- cytes were exposed to OS (20 y 50 μM menadione) for 5 h. Adipogenesis, evaluated by Oil Red O staining as well as by the expression of adipogenic markers (PPARγ, C/EBPα, FAS, FABP4) by Western blot, was observed to be decreased in the chronic mod- el in a menadione concentration-dependent manner (p<0.01). The adipogenic markers were also found to significantly decrease upon acute treatment (p<0.001). However, no significant morphological changes were observed in differentiated adipocytes acutely treated with menadione. PI3K/Akt and ERK1/2 showed to be inversely reg- ulated: Akt was found to be inactivated whereas ERK1/2 were found to be activated in both models of menadione-induced OS. Interest- ingly, experiments in which adipogenesis was evaluated in the pres- ence of LY294002, a pharmacological PI3K inhibitor, but in the ab- sence of menadione showed a significant decreased differentiation. Our results demonstrate that acute menadione-induced OS triggers a gene expression program leading to the decreased expression of transcription factors and enzymes which are crucial for lipogenesis and, on the other hand, chronic menadione-induced OS prevents adipogenesis, presumably in part, in a PI3K-dependent manner. Keywords: adipogenesis, oxidative stress, lipid metabolism.
publishDate 2017
dc.date.none.fl_str_mv 2017
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Effect of oxidative stress on adipocyte differentiation; XIX Reunión Anual De La Sociedad Argentina De Biología; XlVI Reunión Anual De La Sociedad Argentina De Biofísica; Reunión De La Sociedad Argentina De Andrología; LXII Reunión Anual De La Sociedad Argentina De Investigación Clínica; LXV Reunión Anual De La Sociedad Argentina De Inmunología; XlX Reunión Anual De La Sociedad Argentina De Farmacología Experimental; LIII Reunión Anual De La Sociedad Argentina De Investigación Bioquímica y Biología Molecular; Reunión Anual De La Sociedad Argentina De Fisiología y XXIX Reunión Anual De La Sociedad Argentina De Protozoología; Buenos Aires; Argentina; 2017; 397-397
0025-7680
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CONICET Digital
CONICET
url http://hdl.handle.net/11336/232905
identifier_str_mv Effect of oxidative stress on adipocyte differentiation; XIX Reunión Anual De La Sociedad Argentina De Biología; XlVI Reunión Anual De La Sociedad Argentina De Biofísica; Reunión De La Sociedad Argentina De Andrología; LXII Reunión Anual De La Sociedad Argentina De Investigación Clínica; LXV Reunión Anual De La Sociedad Argentina De Inmunología; XlX Reunión Anual De La Sociedad Argentina De Farmacología Experimental; LIII Reunión Anual De La Sociedad Argentina De Investigación Bioquímica y Biología Molecular; Reunión Anual De La Sociedad Argentina De Fisiología y XXIX Reunión Anual De La Sociedad Argentina De Protozoología; Buenos Aires; Argentina; 2017; 397-397
0025-7680
1669-9106
CONICET Digital
CONICET
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publisher.none.fl_str_mv Fundación Revista Medicina
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