Study of a lytic bacteriophage as a tool for the control of Salmonella Gallinarum in layer poultry

Autores
Ortiz, Xoana Pamela; Gismondi, Maria Ines; Barrios, Hebe Alicia
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
One of the leading diseases affecting laying poultry in Argentina is fowl typhoid, caused by the pathogen Salmonella enterica serovar Gallinarum (S. Gallinarum). Although this disease was eradicated from industrial hatcheries in several developed countries, it is still present in commercial farms in Latin America, causing substantial economic losses due to high mortality rates, limited production of quality eggs, high veterinary costs, and the need to renovate infected facilities. To develop a new S. Gallinarum biocontrol method, in the present study, we characterized a bacteriophage in terms of host specificity, growth kinetics, and stability under different environmental conditions. The phage could infect an ample range of bacterial hosts and had no lytic activity against the vaccine strain 9R. Moreover, the phage was stable at pH 3, chloroform presence did not significantly reduce its titer, and its activity was not affected by antibiotics or various chemical agents often used in aviculture. Application of a phage suspension on S. Gallinarum-contaminated surfaces resulted in a significant reduction of bacterial load (p < 0.05). These results show that lytic bacteriophages such as the one studied here are innovative alternatives with potential applications to control S. Gallinarum in laying poultry farms.
Fil: Ortiz, Xoana Pamela. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gismondi, Maria Ines. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
Fil: Barrios, Hebe Alicia. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
Materia
BACTERIOPHAGE
SALMONELLA GALLINARUM
FOWL TYPHOID
LAYING POULTRY
BIOCONTROL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/265459
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/265459
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Study of a lytic bacteriophage as a tool for the control of Salmonella Gallinarum in layer poultryOrtiz, Xoana PamelaGismondi, Maria InesBarrios, Hebe AliciaBACTERIOPHAGESALMONELLA GALLINARUMFOWL TYPHOIDLAYING POULTRYBIOCONTROLhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1One of the leading diseases affecting laying poultry in Argentina is fowl typhoid, caused by the pathogen Salmonella enterica serovar Gallinarum (S. Gallinarum). Although this disease was eradicated from industrial hatcheries in several developed countries, it is still present in commercial farms in Latin America, causing substantial economic losses due to high mortality rates, limited production of quality eggs, high veterinary costs, and the need to renovate infected facilities. To develop a new S. Gallinarum biocontrol method, in the present study, we characterized a bacteriophage in terms of host specificity, growth kinetics, and stability under different environmental conditions. The phage could infect an ample range of bacterial hosts and had no lytic activity against the vaccine strain 9R. Moreover, the phage was stable at pH 3, chloroform presence did not significantly reduce its titer, and its activity was not affected by antibiotics or various chemical agents often used in aviculture. Application of a phage suspension on S. Gallinarum-contaminated surfaces resulted in a significant reduction of bacterial load (p < 0.05). These results show that lytic bacteriophages such as the one studied here are innovative alternatives with potential applications to control S. Gallinarum in laying poultry farms.Fil: Ortiz, Xoana Pamela. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gismondi, Maria Ines. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; ArgentinaFil: Barrios, Hebe Alicia. Universidad Nacional de Luján. Departamento de Ciencias Básicas; ArgentinaPontificia Universidad Javeriana2024-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/265459Ortiz, Xoana Pamela; Gismondi, Maria Ines; Barrios, Hebe Alicia; Study of a lytic bacteriophage as a tool for the control of Salmonella Gallinarum in layer poultry; Pontificia Universidad Javeriana; Universitas Scientiarum; 29; 1; 4-2024; 57-800122-74832027-1352CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://ojspuj.repositoriodigital.com/index.php/scientarium/article/view/37149info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:36:53Zoai:ri.conicet.gov.ar:11336/265459instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:36:54.033CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Study of a lytic bacteriophage as a tool for the control of Salmonella Gallinarum in layer poultry
title Study of a lytic bacteriophage as a tool for the control of Salmonella Gallinarum in layer poultry
spellingShingle Study of a lytic bacteriophage as a tool for the control of Salmonella Gallinarum in layer poultry
Ortiz, Xoana Pamela
BACTERIOPHAGE
SALMONELLA GALLINARUM
FOWL TYPHOID
LAYING POULTRY
BIOCONTROL
title_short Study of a lytic bacteriophage as a tool for the control of Salmonella Gallinarum in layer poultry
title_full Study of a lytic bacteriophage as a tool for the control of Salmonella Gallinarum in layer poultry
title_fullStr Study of a lytic bacteriophage as a tool for the control of Salmonella Gallinarum in layer poultry
title_full_unstemmed Study of a lytic bacteriophage as a tool for the control of Salmonella Gallinarum in layer poultry
title_sort Study of a lytic bacteriophage as a tool for the control of Salmonella Gallinarum in layer poultry
dc.creator.none.fl_str_mv Ortiz, Xoana Pamela
Gismondi, Maria Ines
Barrios, Hebe Alicia
author Ortiz, Xoana Pamela
author_facet Ortiz, Xoana Pamela
Gismondi, Maria Ines
Barrios, Hebe Alicia
author_role author
author2 Gismondi, Maria Ines
Barrios, Hebe Alicia
author2_role author
author
dc.subject.none.fl_str_mv BACTERIOPHAGE
SALMONELLA GALLINARUM
FOWL TYPHOID
LAYING POULTRY
BIOCONTROL
topic BACTERIOPHAGE
SALMONELLA GALLINARUM
FOWL TYPHOID
LAYING POULTRY
BIOCONTROL
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv One of the leading diseases affecting laying poultry in Argentina is fowl typhoid, caused by the pathogen Salmonella enterica serovar Gallinarum (S. Gallinarum). Although this disease was eradicated from industrial hatcheries in several developed countries, it is still present in commercial farms in Latin America, causing substantial economic losses due to high mortality rates, limited production of quality eggs, high veterinary costs, and the need to renovate infected facilities. To develop a new S. Gallinarum biocontrol method, in the present study, we characterized a bacteriophage in terms of host specificity, growth kinetics, and stability under different environmental conditions. The phage could infect an ample range of bacterial hosts and had no lytic activity against the vaccine strain 9R. Moreover, the phage was stable at pH 3, chloroform presence did not significantly reduce its titer, and its activity was not affected by antibiotics or various chemical agents often used in aviculture. Application of a phage suspension on S. Gallinarum-contaminated surfaces resulted in a significant reduction of bacterial load (p < 0.05). These results show that lytic bacteriophages such as the one studied here are innovative alternatives with potential applications to control S. Gallinarum in laying poultry farms.
Fil: Ortiz, Xoana Pamela. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gismondi, Maria Ines. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
Fil: Barrios, Hebe Alicia. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
description One of the leading diseases affecting laying poultry in Argentina is fowl typhoid, caused by the pathogen Salmonella enterica serovar Gallinarum (S. Gallinarum). Although this disease was eradicated from industrial hatcheries in several developed countries, it is still present in commercial farms in Latin America, causing substantial economic losses due to high mortality rates, limited production of quality eggs, high veterinary costs, and the need to renovate infected facilities. To develop a new S. Gallinarum biocontrol method, in the present study, we characterized a bacteriophage in terms of host specificity, growth kinetics, and stability under different environmental conditions. The phage could infect an ample range of bacterial hosts and had no lytic activity against the vaccine strain 9R. Moreover, the phage was stable at pH 3, chloroform presence did not significantly reduce its titer, and its activity was not affected by antibiotics or various chemical agents often used in aviculture. Application of a phage suspension on S. Gallinarum-contaminated surfaces resulted in a significant reduction of bacterial load (p < 0.05). These results show that lytic bacteriophages such as the one studied here are innovative alternatives with potential applications to control S. Gallinarum in laying poultry farms.
publishDate 2024
dc.date.none.fl_str_mv 2024-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/265459
Ortiz, Xoana Pamela; Gismondi, Maria Ines; Barrios, Hebe Alicia; Study of a lytic bacteriophage as a tool for the control of Salmonella Gallinarum in layer poultry; Pontificia Universidad Javeriana; Universitas Scientiarum; 29; 1; 4-2024; 57-80
0122-7483
2027-1352
CONICET Digital
CONICET
url http://hdl.handle.net/11336/265459
identifier_str_mv Ortiz, Xoana Pamela; Gismondi, Maria Ines; Barrios, Hebe Alicia; Study of a lytic bacteriophage as a tool for the control of Salmonella Gallinarum in layer poultry; Pontificia Universidad Javeriana; Universitas Scientiarum; 29; 1; 4-2024; 57-80
0122-7483
2027-1352
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ojspuj.repositoriodigital.com/index.php/scientarium/article/view/37149
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pontificia Universidad Javeriana
publisher.none.fl_str_mv Pontificia Universidad Javeriana
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613159964377088
score 13.070432

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