Comparison of 3 culture methods and PCR assays for Salmonella gallinarum and Salmonella pullorum detection in poultry feed

Autores
Soria, Maria Cecilia; Soria, Mario; Bueno, Dante Javier; Terzolo, Horacio
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To detect Salmonella gallinarum or Salmonella pullorum in artificially contaminated poultry feed, 9 culture combinations were compared, including 3 preenrichment/enrichment methods (tryptic soy broth plus ferrous sulfate/tetrathionate Hajna, tryptic soy broth plus ferrous sulfate/selenite cystine broth, and Salmosyst) in combination with 3 selective agars (xylose lysine desoxicholate agar added with tergitol 4, EF-18, and Önöz), respectively. Additionally, a single PCR technique was applied combined with 2 different preenrichment media (tryptic soy broth plus ferrous sulfate and Salmosyst). The specificity and positive predictive value were 1 for all methods. There were some differences among Salmonella strains for sensitivity and accuracy in the culture and Salmosyst-PCR methods. The sensitivity and accuracy values were less than 0.60 and 0.64, respectively, whereas the negative predictive values were between 0.12 and 0.23. Two PCR methods did not show any difference in the parameters of performance evaluated. Kappa coefficients showed good agreement between both methods. None of the culture combinations was able to detect S. gallinarum or S. pullorum when the inoculum was less than 3 × 102 cfu/25 g, except the Salmosyst broth method, which could recover S. gallinarum from 3 × 101 cfu/25 g onward. Overall, there were differences in the detection limits among the strains and methods used. In general, the 3 selective plating media did not show any significant difference in the parameters of performance studied for each strain. On the other hand, the agreements were slight to fair when culture methods were compared among them and with both PCR methods. The differences in the detection levels that were obtained using these methods and the difficulty in detecting S. gallinarum or S. pullorum in feed represent a potential problem when a poultry feed sample is considered to be negative. It is highly recommended to use at least 2 methods to increase the chances of detecting S. gallinarum or S. pullorum in poultry feed.
EEA Concepción del Uruguay
Fil: Soria, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Terzolo, Horacio Raúl. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fuente
Poultry Science 92 (6) : 1505–1515 (June 2013)
Materia
Enfermedades de los Animales
Aves de Corral
Salmonella gallinarum
Salmonella pullorum
PCR
Técnicas de Cultivo
Alimentación Avícola
Animal Diseases
Poultry
Culture Techniques
Poultry Feeding
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/6463
Acceder
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oai_identifier_str oai:localhost:20.500.12123/6463
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Comparison of 3 culture methods and PCR assays for Salmonella gallinarum and Salmonella pullorum detection in poultry feedSoria, Maria CeciliaSoria, MarioBueno, Dante JavierTerzolo, HoracioEnfermedades de los AnimalesAves de CorralSalmonella gallinarumSalmonella pullorumPCRTécnicas de CultivoAlimentación AvícolaAnimal DiseasesPoultryCulture TechniquesPoultry FeedingTo detect Salmonella gallinarum or Salmonella pullorum in artificially contaminated poultry feed, 9 culture combinations were compared, including 3 preenrichment/enrichment methods (tryptic soy broth plus ferrous sulfate/tetrathionate Hajna, tryptic soy broth plus ferrous sulfate/selenite cystine broth, and Salmosyst) in combination with 3 selective agars (xylose lysine desoxicholate agar added with tergitol 4, EF-18, and Önöz), respectively. Additionally, a single PCR technique was applied combined with 2 different preenrichment media (tryptic soy broth plus ferrous sulfate and Salmosyst). The specificity and positive predictive value were 1 for all methods. There were some differences among Salmonella strains for sensitivity and accuracy in the culture and Salmosyst-PCR methods. The sensitivity and accuracy values were less than 0.60 and 0.64, respectively, whereas the negative predictive values were between 0.12 and 0.23. Two PCR methods did not show any difference in the parameters of performance evaluated. Kappa coefficients showed good agreement between both methods. None of the culture combinations was able to detect S. gallinarum or S. pullorum when the inoculum was less than 3 × 102 cfu/25 g, except the Salmosyst broth method, which could recover S. gallinarum from 3 × 101 cfu/25 g onward. Overall, there were differences in the detection limits among the strains and methods used. In general, the 3 selective plating media did not show any significant difference in the parameters of performance studied for each strain. On the other hand, the agreements were slight to fair when culture methods were compared among them and with both PCR methods. The differences in the detection levels that were obtained using these methods and the difficulty in detecting S. gallinarum or S. pullorum in feed represent a potential problem when a poultry feed sample is considered to be negative. It is highly recommended to use at least 2 methods to increase the chances of detecting S. gallinarum or S. pullorum in poultry feed.EEA Concepción del UruguayFil: Soria, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Terzolo, Horacio Raúl. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Oxford Academic Press2019-12-06T11:59:16Z2019-12-06T11:59:16Z2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://academic.oup.com/ps/article/92/6/1505/1592721http://hdl.handle.net/20.500.12123/64630032-57911525-3171https://doi.org/10.3382/ps.2012-02926Poultry Science 92 (6) : 1505–1515 (June 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:50Zoai:localhost:20.500.12123/6463instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:50.823INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Comparison of 3 culture methods and PCR assays for Salmonella gallinarum and Salmonella pullorum detection in poultry feed
title Comparison of 3 culture methods and PCR assays for Salmonella gallinarum and Salmonella pullorum detection in poultry feed
spellingShingle Comparison of 3 culture methods and PCR assays for Salmonella gallinarum and Salmonella pullorum detection in poultry feed
Soria, Maria Cecilia
Enfermedades de los Animales
Aves de Corral
Salmonella gallinarum
Salmonella pullorum
PCR
Técnicas de Cultivo
Alimentación Avícola
Animal Diseases
Poultry
Culture Techniques
Poultry Feeding
title_short Comparison of 3 culture methods and PCR assays for Salmonella gallinarum and Salmonella pullorum detection in poultry feed
title_full Comparison of 3 culture methods and PCR assays for Salmonella gallinarum and Salmonella pullorum detection in poultry feed
title_fullStr Comparison of 3 culture methods and PCR assays for Salmonella gallinarum and Salmonella pullorum detection in poultry feed
title_full_unstemmed Comparison of 3 culture methods and PCR assays for Salmonella gallinarum and Salmonella pullorum detection in poultry feed
title_sort Comparison of 3 culture methods and PCR assays for Salmonella gallinarum and Salmonella pullorum detection in poultry feed
dc.creator.none.fl_str_mv Soria, Maria Cecilia
Soria, Mario
Bueno, Dante Javier
Terzolo, Horacio
author Soria, Maria Cecilia
author_facet Soria, Maria Cecilia
Soria, Mario
Bueno, Dante Javier
Terzolo, Horacio
author_role author
author2 Soria, Mario
Bueno, Dante Javier
Terzolo, Horacio
author2_role author
author
author
dc.subject.none.fl_str_mv Enfermedades de los Animales
Aves de Corral
Salmonella gallinarum
Salmonella pullorum
PCR
Técnicas de Cultivo
Alimentación Avícola
Animal Diseases
Poultry
Culture Techniques
Poultry Feeding
topic Enfermedades de los Animales
Aves de Corral
Salmonella gallinarum
Salmonella pullorum
PCR
Técnicas de Cultivo
Alimentación Avícola
Animal Diseases
Poultry
Culture Techniques
Poultry Feeding
dc.description.none.fl_txt_mv To detect Salmonella gallinarum or Salmonella pullorum in artificially contaminated poultry feed, 9 culture combinations were compared, including 3 preenrichment/enrichment methods (tryptic soy broth plus ferrous sulfate/tetrathionate Hajna, tryptic soy broth plus ferrous sulfate/selenite cystine broth, and Salmosyst) in combination with 3 selective agars (xylose lysine desoxicholate agar added with tergitol 4, EF-18, and Önöz), respectively. Additionally, a single PCR technique was applied combined with 2 different preenrichment media (tryptic soy broth plus ferrous sulfate and Salmosyst). The specificity and positive predictive value were 1 for all methods. There were some differences among Salmonella strains for sensitivity and accuracy in the culture and Salmosyst-PCR methods. The sensitivity and accuracy values were less than 0.60 and 0.64, respectively, whereas the negative predictive values were between 0.12 and 0.23. Two PCR methods did not show any difference in the parameters of performance evaluated. Kappa coefficients showed good agreement between both methods. None of the culture combinations was able to detect S. gallinarum or S. pullorum when the inoculum was less than 3 × 102 cfu/25 g, except the Salmosyst broth method, which could recover S. gallinarum from 3 × 101 cfu/25 g onward. Overall, there were differences in the detection limits among the strains and methods used. In general, the 3 selective plating media did not show any significant difference in the parameters of performance studied for each strain. On the other hand, the agreements were slight to fair when culture methods were compared among them and with both PCR methods. The differences in the detection levels that were obtained using these methods and the difficulty in detecting S. gallinarum or S. pullorum in feed represent a potential problem when a poultry feed sample is considered to be negative. It is highly recommended to use at least 2 methods to increase the chances of detecting S. gallinarum or S. pullorum in poultry feed.
EEA Concepción del Uruguay
Fil: Soria, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Terzolo, Horacio Raúl. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
description To detect Salmonella gallinarum or Salmonella pullorum in artificially contaminated poultry feed, 9 culture combinations were compared, including 3 preenrichment/enrichment methods (tryptic soy broth plus ferrous sulfate/tetrathionate Hajna, tryptic soy broth plus ferrous sulfate/selenite cystine broth, and Salmosyst) in combination with 3 selective agars (xylose lysine desoxicholate agar added with tergitol 4, EF-18, and Önöz), respectively. Additionally, a single PCR technique was applied combined with 2 different preenrichment media (tryptic soy broth plus ferrous sulfate and Salmosyst). The specificity and positive predictive value were 1 for all methods. There were some differences among Salmonella strains for sensitivity and accuracy in the culture and Salmosyst-PCR methods. The sensitivity and accuracy values were less than 0.60 and 0.64, respectively, whereas the negative predictive values were between 0.12 and 0.23. Two PCR methods did not show any difference in the parameters of performance evaluated. Kappa coefficients showed good agreement between both methods. None of the culture combinations was able to detect S. gallinarum or S. pullorum when the inoculum was less than 3 × 102 cfu/25 g, except the Salmosyst broth method, which could recover S. gallinarum from 3 × 101 cfu/25 g onward. Overall, there were differences in the detection limits among the strains and methods used. In general, the 3 selective plating media did not show any significant difference in the parameters of performance studied for each strain. On the other hand, the agreements were slight to fair when culture methods were compared among them and with both PCR methods. The differences in the detection levels that were obtained using these methods and the difficulty in detecting S. gallinarum or S. pullorum in feed represent a potential problem when a poultry feed sample is considered to be negative. It is highly recommended to use at least 2 methods to increase the chances of detecting S. gallinarum or S. pullorum in poultry feed.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
2019-12-06T11:59:16Z
2019-12-06T11:59:16Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://academic.oup.com/ps/article/92/6/1505/1592721
http://hdl.handle.net/20.500.12123/6463
0032-5791
1525-3171
https://doi.org/10.3382/ps.2012-02926
url https://academic.oup.com/ps/article/92/6/1505/1592721
http://hdl.handle.net/20.500.12123/6463
https://doi.org/10.3382/ps.2012-02926
identifier_str_mv 0032-5791
1525-3171
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford Academic Press
publisher.none.fl_str_mv Oxford Academic Press
dc.source.none.fl_str_mv Poultry Science 92 (6) : 1505–1515 (June 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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