Molecular identification and pathogenicity of Rhizoctonia spp. recovered from seed and soil samples of the main bean growing area of Argentina

Autores
Spedaletti, Yamila Andrea; Mercado Cárdenas, Guadalupe Eugenia; Taboada, Gisel María; Aban, Carla Luciana; Aparicio González, Mónica Alicia; Rodriguero, Marcela Silvia; Vizgarra, Oscar Niceforo; Sühring, Silvia Susana; Galindez, Guadalupe; Galván, Marta Zulema
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objective of this study was to perform the molecular and pathogenic characterization of Rhizoctonia solani in bean seed and soil samples from fields with root rot symptoms in northwestern Argentina. Fifty-two Rhizoctonia spp. isolates were collected in ten naturally infested bean fields and characterized by morphological traits, DNA sequencing, and pathogenicity. The mycelium color, the pattern of sclerotia formation, and the number of nuclei per hyphal cell were determined for each isolate. According to the variability in the rDNA-ITS region, isolates were identified as R. solani (85%), Waitea circinata var. zeae (Rhizoctonia zeae) (2%) and Rhizoctonia spp. (13%). Most isolates of R. solani (92%) were found to belong to the anastomosis group (AG) AG 4, including seven AG 4 HG-I and nine AG 4 HG-III isolates. AGs obtained from soil samples were more variable than those obtained from seed samples. Molecular identification of the isolates was in agreement with their morphological characterization. In addition, aggressiveness of the isolates towards bean seedlings was assessed in the greenhouse. Four virulence categories were defined according to the disease reaction on root and foliar tissues, which showed great variability in virulence among the isolates. Our results suggest that both seed and soil-borne inoculum may play a significant role in pathogen dispersal in the region. This is the first study on Rhizoctonia species and AGs in bean seed and soil in this region and it may contribute towards an efficient control strategy for bean diseases caused by Rhizoctonia species.
Fil: Spedaletti, Yamila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina
Fil: Mercado Cárdenas, Guadalupe Eugenia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina
Fil: Taboada, Gisel María. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina
Fil: Aban, Carla Luciana. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina
Fil: Aparicio González, Mónica Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina
Fil: Rodriguero, Marcela Silvia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución. Laboratorio de Ecología Funcional; Argentina
Fil: Vizgarra, Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial "Obispo Colombres" (p). Instituto de Tecnología Agroindustrial del Noroeste Argentino; Argentina
Fil: Sühring, Silvia Susana. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina
Fil: Galindez, Guadalupe. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina
Fil: Galván, Marta Zulema. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina
Materia
ANASTOMOSIS GROUP
RDNA-LTS
PHASEOLUS VULGARIS
ROOT ROT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/93462
Acceder
id CONICETDig_f08e4598a414e6743937b3919eb813ff
oai_identifier_str oai:ri.conicet.gov.ar:11336/93462
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular identification and pathogenicity of Rhizoctonia spp. recovered from seed and soil samples of the main bean growing area of ArgentinaSpedaletti, Yamila AndreaMercado Cárdenas, Guadalupe EugeniaTaboada, Gisel MaríaAban, Carla LucianaAparicio González, Mónica AliciaRodriguero, Marcela SilviaVizgarra, Oscar NiceforoSühring, Silvia SusanaGalindez, GuadalupeGalván, Marta ZulemaANASTOMOSIS GROUPRDNA-LTSPHASEOLUS VULGARISROOT ROThttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4The objective of this study was to perform the molecular and pathogenic characterization of Rhizoctonia solani in bean seed and soil samples from fields with root rot symptoms in northwestern Argentina. Fifty-two Rhizoctonia spp. isolates were collected in ten naturally infested bean fields and characterized by morphological traits, DNA sequencing, and pathogenicity. The mycelium color, the pattern of sclerotia formation, and the number of nuclei per hyphal cell were determined for each isolate. According to the variability in the rDNA-ITS region, isolates were identified as R. solani (85%), Waitea circinata var. zeae (Rhizoctonia zeae) (2%) and Rhizoctonia spp. (13%). Most isolates of R. solani (92%) were found to belong to the anastomosis group (AG) AG 4, including seven AG 4 HG-I and nine AG 4 HG-III isolates. AGs obtained from soil samples were more variable than those obtained from seed samples. Molecular identification of the isolates was in agreement with their morphological characterization. In addition, aggressiveness of the isolates towards bean seedlings was assessed in the greenhouse. Four virulence categories were defined according to the disease reaction on root and foliar tissues, which showed great variability in virulence among the isolates. Our results suggest that both seed and soil-borne inoculum may play a significant role in pathogen dispersal in the region. This is the first study on Rhizoctonia species and AGs in bean seed and soil in this region and it may contribute towards an efficient control strategy for bean diseases caused by Rhizoctonia species.Fil: Spedaletti, Yamila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; ArgentinaFil: Mercado Cárdenas, Guadalupe Eugenia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; ArgentinaFil: Taboada, Gisel María. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; ArgentinaFil: Aban, Carla Luciana. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; ArgentinaFil: Aparicio González, Mónica Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; ArgentinaFil: Rodriguero, Marcela Silvia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución. Laboratorio de Ecología Funcional; ArgentinaFil: Vizgarra, Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial "Obispo Colombres" (p). Instituto de Tecnología Agroindustrial del Noroeste Argentino; ArgentinaFil: Sühring, Silvia Susana. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaFil: Galindez, Guadalupe. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaFil: Galván, Marta Zulema. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; ArgentinaSouthern Cross Publishing2017-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/93462Spedaletti, Yamila Andrea; Mercado Cárdenas, Guadalupe Eugenia; Taboada, Gisel María; Aban, Carla Luciana; Aparicio González, Mónica Alicia; et al.; Molecular identification and pathogenicity of Rhizoctonia spp. recovered from seed and soil samples of the main bean growing area of Argentina; Southern Cross Publishing; Australian Journal of Crop Science; 11; 08; 8-2017; 952-9591835-26931835-2707CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.cropj.com/galvan_11_8_2017_952_959.pdfinfo:eu-repo/semantics/altIdentifier/doi/10.21475/ajcs.17.11.08.pne432info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:03:46Zoai:ri.conicet.gov.ar:11336/93462instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:03:46.617CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular identification and pathogenicity of Rhizoctonia spp. recovered from seed and soil samples of the main bean growing area of Argentina
title Molecular identification and pathogenicity of Rhizoctonia spp. recovered from seed and soil samples of the main bean growing area of Argentina
spellingShingle Molecular identification and pathogenicity of Rhizoctonia spp. recovered from seed and soil samples of the main bean growing area of Argentina
Spedaletti, Yamila Andrea
ANASTOMOSIS GROUP
RDNA-LTS
PHASEOLUS VULGARIS
ROOT ROT
title_short Molecular identification and pathogenicity of Rhizoctonia spp. recovered from seed and soil samples of the main bean growing area of Argentina
title_full Molecular identification and pathogenicity of Rhizoctonia spp. recovered from seed and soil samples of the main bean growing area of Argentina
title_fullStr Molecular identification and pathogenicity of Rhizoctonia spp. recovered from seed and soil samples of the main bean growing area of Argentina
title_full_unstemmed Molecular identification and pathogenicity of Rhizoctonia spp. recovered from seed and soil samples of the main bean growing area of Argentina
title_sort Molecular identification and pathogenicity of Rhizoctonia spp. recovered from seed and soil samples of the main bean growing area of Argentina
dc.creator.none.fl_str_mv Spedaletti, Yamila Andrea
Mercado Cárdenas, Guadalupe Eugenia
Taboada, Gisel María
Aban, Carla Luciana
Aparicio González, Mónica Alicia
Rodriguero, Marcela Silvia
Vizgarra, Oscar Niceforo
Sühring, Silvia Susana
Galindez, Guadalupe
Galván, Marta Zulema
author Spedaletti, Yamila Andrea
author_facet Spedaletti, Yamila Andrea
Mercado Cárdenas, Guadalupe Eugenia
Taboada, Gisel María
Aban, Carla Luciana
Aparicio González, Mónica Alicia
Rodriguero, Marcela Silvia
Vizgarra, Oscar Niceforo
Sühring, Silvia Susana
Galindez, Guadalupe
Galván, Marta Zulema
author_role author
author2 Mercado Cárdenas, Guadalupe Eugenia
Taboada, Gisel María
Aban, Carla Luciana
Aparicio González, Mónica Alicia
Rodriguero, Marcela Silvia
Vizgarra, Oscar Niceforo
Sühring, Silvia Susana
Galindez, Guadalupe
Galván, Marta Zulema
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANASTOMOSIS GROUP
RDNA-LTS
PHASEOLUS VULGARIS
ROOT ROT
topic ANASTOMOSIS GROUP
RDNA-LTS
PHASEOLUS VULGARIS
ROOT ROT
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The objective of this study was to perform the molecular and pathogenic characterization of Rhizoctonia solani in bean seed and soil samples from fields with root rot symptoms in northwestern Argentina. Fifty-two Rhizoctonia spp. isolates were collected in ten naturally infested bean fields and characterized by morphological traits, DNA sequencing, and pathogenicity. The mycelium color, the pattern of sclerotia formation, and the number of nuclei per hyphal cell were determined for each isolate. According to the variability in the rDNA-ITS region, isolates were identified as R. solani (85%), Waitea circinata var. zeae (Rhizoctonia zeae) (2%) and Rhizoctonia spp. (13%). Most isolates of R. solani (92%) were found to belong to the anastomosis group (AG) AG 4, including seven AG 4 HG-I and nine AG 4 HG-III isolates. AGs obtained from soil samples were more variable than those obtained from seed samples. Molecular identification of the isolates was in agreement with their morphological characterization. In addition, aggressiveness of the isolates towards bean seedlings was assessed in the greenhouse. Four virulence categories were defined according to the disease reaction on root and foliar tissues, which showed great variability in virulence among the isolates. Our results suggest that both seed and soil-borne inoculum may play a significant role in pathogen dispersal in the region. This is the first study on Rhizoctonia species and AGs in bean seed and soil in this region and it may contribute towards an efficient control strategy for bean diseases caused by Rhizoctonia species.
Fil: Spedaletti, Yamila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina
Fil: Mercado Cárdenas, Guadalupe Eugenia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina
Fil: Taboada, Gisel María. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina
Fil: Aban, Carla Luciana. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina
Fil: Aparicio González, Mónica Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina
Fil: Rodriguero, Marcela Silvia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución. Laboratorio de Ecología Funcional; Argentina
Fil: Vizgarra, Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial "Obispo Colombres" (p). Instituto de Tecnología Agroindustrial del Noroeste Argentino; Argentina
Fil: Sühring, Silvia Susana. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina
Fil: Galindez, Guadalupe. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina
Fil: Galván, Marta Zulema. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina
description The objective of this study was to perform the molecular and pathogenic characterization of Rhizoctonia solani in bean seed and soil samples from fields with root rot symptoms in northwestern Argentina. Fifty-two Rhizoctonia spp. isolates were collected in ten naturally infested bean fields and characterized by morphological traits, DNA sequencing, and pathogenicity. The mycelium color, the pattern of sclerotia formation, and the number of nuclei per hyphal cell were determined for each isolate. According to the variability in the rDNA-ITS region, isolates were identified as R. solani (85%), Waitea circinata var. zeae (Rhizoctonia zeae) (2%) and Rhizoctonia spp. (13%). Most isolates of R. solani (92%) were found to belong to the anastomosis group (AG) AG 4, including seven AG 4 HG-I and nine AG 4 HG-III isolates. AGs obtained from soil samples were more variable than those obtained from seed samples. Molecular identification of the isolates was in agreement with their morphological characterization. In addition, aggressiveness of the isolates towards bean seedlings was assessed in the greenhouse. Four virulence categories were defined according to the disease reaction on root and foliar tissues, which showed great variability in virulence among the isolates. Our results suggest that both seed and soil-borne inoculum may play a significant role in pathogen dispersal in the region. This is the first study on Rhizoctonia species and AGs in bean seed and soil in this region and it may contribute towards an efficient control strategy for bean diseases caused by Rhizoctonia species.
publishDate 2017
dc.date.none.fl_str_mv 2017-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/93462
Spedaletti, Yamila Andrea; Mercado Cárdenas, Guadalupe Eugenia; Taboada, Gisel María; Aban, Carla Luciana; Aparicio González, Mónica Alicia; et al.; Molecular identification and pathogenicity of Rhizoctonia spp. recovered from seed and soil samples of the main bean growing area of Argentina; Southern Cross Publishing; Australian Journal of Crop Science; 11; 08; 8-2017; 952-959
1835-2693
1835-2707
CONICET Digital
CONICET
url http://hdl.handle.net/11336/93462
identifier_str_mv Spedaletti, Yamila Andrea; Mercado Cárdenas, Guadalupe Eugenia; Taboada, Gisel María; Aban, Carla Luciana; Aparicio González, Mónica Alicia; et al.; Molecular identification and pathogenicity of Rhizoctonia spp. recovered from seed and soil samples of the main bean growing area of Argentina; Southern Cross Publishing; Australian Journal of Crop Science; 11; 08; 8-2017; 952-959
1835-2693
1835-2707
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.cropj.com/galvan_11_8_2017_952_959.pdf
info:eu-repo/semantics/altIdentifier/doi/10.21475/ajcs.17.11.08.pne432
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Southern Cross Publishing
publisher.none.fl_str_mv Southern Cross Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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