Molecular characterization and pathogenicity of Rhizoctonia spp. isolates from a tobacco growing area in northwestern Argentina

Autores
Mercado Cardenas, Guadalupe E.; Galván, Marta Zulema; Barrera, Viviana A.; Rodriguero, Marcela Silvina; Carmona, Marcelo A.; March, Guillermo J.; Ramallo, Ana C.; Shew, H. David
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In Argentina, over 60% of the tobacco crop is grown in the northwestern part of the country. In this region, Rhizoctonia solani can cause serious disease problems that result in losses in yield and quality. We collected 35 isolates of Rhizoctonia from 32 tobacco fields in Northwestern Argentina and characterized the isolates by means of morphological and molecular approaches. Based on variability in the ITS region, isolates were identified as R. solani (80%), Waitea circinata var. zeae (Rhizoctonia zeae) (8%) and binucleate Rhizoctonia (8%). Most isolates of R. solani belonged to the anastomosis groups (AG) AG 4 HG-I (44%), AG 2-1 (41%) and AG 4 HG-III (13%). Isolates of binucleate Rhizoctonia belonged to AG-F and AG-P of Ceratobasidium sp. Greater morphological variability was observed within isolates of AG 2-1 and AG 4 HG-III than in isolates of AG 4 HG-I. The aggressiveness of isolates on tobacco seedlings was determined under greenhouse conditions. Isolates of AG 2-1 were the most aggressive on leaves (target spot), while isolates of AG 4 HG-I were the most aggressive on stems and roots, causing damping-off. This is the first in depth study of Rhizoctonia on Virginia tobacco cultivars in the major production areas of Argentina.
Fil: Mercado Cardenas, Guadalupe E.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina
Fil: Galván, Marta Zulema. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta; Argentina
Fil: Barrera, Viviana A.. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Rodriguero, Marcela Silvina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Carmona, Marcelo A.. Universidad de Buenos Aires. Facultad de Agronomia; Argentina
Fil: March, Guillermo J.. Instituto Nacional de Tecnologi­a Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Instituto de Patologi­a Vegetal; Argentina
Fil: Ramallo, Ana C.. Universidad Nacional de Tucumán; Argentina
Fil: Shew, H. David. University Of North Carolina; Estados Unidos
Materia
Nicotiana Tabacum
Its
Anastomosis Group (Ag)
Vegetative Compatibility
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7151
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/7151
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular characterization and pathogenicity of Rhizoctonia spp. isolates from a tobacco growing area in northwestern ArgentinaMercado Cardenas, Guadalupe E.Galván, Marta ZulemaBarrera, Viviana A.Rodriguero, Marcela SilvinaCarmona, Marcelo A.March, Guillermo J.Ramallo, Ana C.Shew, H. DavidNicotiana TabacumItsAnastomosis Group (Ag)Vegetative Compatibilityhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4In Argentina, over 60% of the tobacco crop is grown in the northwestern part of the country. In this region, Rhizoctonia solani can cause serious disease problems that result in losses in yield and quality. We collected 35 isolates of Rhizoctonia from 32 tobacco fields in Northwestern Argentina and characterized the isolates by means of morphological and molecular approaches. Based on variability in the ITS region, isolates were identified as R. solani (80%), Waitea circinata var. zeae (Rhizoctonia zeae) (8%) and binucleate Rhizoctonia (8%). Most isolates of R. solani belonged to the anastomosis groups (AG) AG 4 HG-I (44%), AG 2-1 (41%) and AG 4 HG-III (13%). Isolates of binucleate Rhizoctonia belonged to AG-F and AG-P of Ceratobasidium sp. Greater morphological variability was observed within isolates of AG 2-1 and AG 4 HG-III than in isolates of AG 4 HG-I. The aggressiveness of isolates on tobacco seedlings was determined under greenhouse conditions. Isolates of AG 2-1 were the most aggressive on leaves (target spot), while isolates of AG 4 HG-I were the most aggressive on stems and roots, causing damping-off. This is the first in depth study of Rhizoctonia on Virginia tobacco cultivars in the major production areas of Argentina.Fil: Mercado Cardenas, Guadalupe E.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; ArgentinaFil: Galván, Marta Zulema. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta; ArgentinaFil: Barrera, Viviana A.. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Rodriguero, Marcela Silvina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: Carmona, Marcelo A.. Universidad de Buenos Aires. Facultad de Agronomia; ArgentinaFil: March, Guillermo J.. Instituto Nacional de Tecnologi­a Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Instituto de Patologi­a Vegetal; ArgentinaFil: Ramallo, Ana C.. Universidad Nacional de Tucumán; ArgentinaFil: Shew, H. David. University Of North Carolina; Estados UnidosSociedade Brasileira de Fitopatologia2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7151Mercado Cardenas, Guadalupe E.; Galván, Marta Zulema; Barrera, Viviana A.; Rodriguero, Marcela Silvina; Carmona, Marcelo A.; et al.; Molecular characterization and pathogenicity of Rhizoctonia spp. isolates from a tobacco growing area in northwestern Argentina; Sociedade Brasileira de Fitopatologia; Tropical Plant Pathology; 40; 3; 7-2015; 160-1681982-56761983-2052enginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s40858-015-0035-7info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s40858-015-0035-7info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:35:54Zoai:ri.conicet.gov.ar:11336/7151instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:35:54.663CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular characterization and pathogenicity of Rhizoctonia spp. isolates from a tobacco growing area in northwestern Argentina
title Molecular characterization and pathogenicity of Rhizoctonia spp. isolates from a tobacco growing area in northwestern Argentina
spellingShingle Molecular characterization and pathogenicity of Rhizoctonia spp. isolates from a tobacco growing area in northwestern Argentina
Mercado Cardenas, Guadalupe E.
Nicotiana Tabacum
Its
Anastomosis Group (Ag)
Vegetative Compatibility
title_short Molecular characterization and pathogenicity of Rhizoctonia spp. isolates from a tobacco growing area in northwestern Argentina
title_full Molecular characterization and pathogenicity of Rhizoctonia spp. isolates from a tobacco growing area in northwestern Argentina
title_fullStr Molecular characterization and pathogenicity of Rhizoctonia spp. isolates from a tobacco growing area in northwestern Argentina
title_full_unstemmed Molecular characterization and pathogenicity of Rhizoctonia spp. isolates from a tobacco growing area in northwestern Argentina
title_sort Molecular characterization and pathogenicity of Rhizoctonia spp. isolates from a tobacco growing area in northwestern Argentina
dc.creator.none.fl_str_mv Mercado Cardenas, Guadalupe E.
Galván, Marta Zulema
Barrera, Viviana A.
Rodriguero, Marcela Silvina
Carmona, Marcelo A.
March, Guillermo J.
Ramallo, Ana C.
Shew, H. David
author Mercado Cardenas, Guadalupe E.
author_facet Mercado Cardenas, Guadalupe E.
Galván, Marta Zulema
Barrera, Viviana A.
Rodriguero, Marcela Silvina
Carmona, Marcelo A.
March, Guillermo J.
Ramallo, Ana C.
Shew, H. David
author_role author
author2 Galván, Marta Zulema
Barrera, Viviana A.
Rodriguero, Marcela Silvina
Carmona, Marcelo A.
March, Guillermo J.
Ramallo, Ana C.
Shew, H. David
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Nicotiana Tabacum
Its
Anastomosis Group (Ag)
Vegetative Compatibility
topic Nicotiana Tabacum
Its
Anastomosis Group (Ag)
Vegetative Compatibility
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv In Argentina, over 60% of the tobacco crop is grown in the northwestern part of the country. In this region, Rhizoctonia solani can cause serious disease problems that result in losses in yield and quality. We collected 35 isolates of Rhizoctonia from 32 tobacco fields in Northwestern Argentina and characterized the isolates by means of morphological and molecular approaches. Based on variability in the ITS region, isolates were identified as R. solani (80%), Waitea circinata var. zeae (Rhizoctonia zeae) (8%) and binucleate Rhizoctonia (8%). Most isolates of R. solani belonged to the anastomosis groups (AG) AG 4 HG-I (44%), AG 2-1 (41%) and AG 4 HG-III (13%). Isolates of binucleate Rhizoctonia belonged to AG-F and AG-P of Ceratobasidium sp. Greater morphological variability was observed within isolates of AG 2-1 and AG 4 HG-III than in isolates of AG 4 HG-I. The aggressiveness of isolates on tobacco seedlings was determined under greenhouse conditions. Isolates of AG 2-1 were the most aggressive on leaves (target spot), while isolates of AG 4 HG-I were the most aggressive on stems and roots, causing damping-off. This is the first in depth study of Rhizoctonia on Virginia tobacco cultivars in the major production areas of Argentina.
Fil: Mercado Cardenas, Guadalupe E.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina
Fil: Galván, Marta Zulema. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta; Argentina
Fil: Barrera, Viviana A.. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Rodriguero, Marcela Silvina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Carmona, Marcelo A.. Universidad de Buenos Aires. Facultad de Agronomia; Argentina
Fil: March, Guillermo J.. Instituto Nacional de Tecnologi­a Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Instituto de Patologi­a Vegetal; Argentina
Fil: Ramallo, Ana C.. Universidad Nacional de Tucumán; Argentina
Fil: Shew, H. David. University Of North Carolina; Estados Unidos
description In Argentina, over 60% of the tobacco crop is grown in the northwestern part of the country. In this region, Rhizoctonia solani can cause serious disease problems that result in losses in yield and quality. We collected 35 isolates of Rhizoctonia from 32 tobacco fields in Northwestern Argentina and characterized the isolates by means of morphological and molecular approaches. Based on variability in the ITS region, isolates were identified as R. solani (80%), Waitea circinata var. zeae (Rhizoctonia zeae) (8%) and binucleate Rhizoctonia (8%). Most isolates of R. solani belonged to the anastomosis groups (AG) AG 4 HG-I (44%), AG 2-1 (41%) and AG 4 HG-III (13%). Isolates of binucleate Rhizoctonia belonged to AG-F and AG-P of Ceratobasidium sp. Greater morphological variability was observed within isolates of AG 2-1 and AG 4 HG-III than in isolates of AG 4 HG-I. The aggressiveness of isolates on tobacco seedlings was determined under greenhouse conditions. Isolates of AG 2-1 were the most aggressive on leaves (target spot), while isolates of AG 4 HG-I were the most aggressive on stems and roots, causing damping-off. This is the first in depth study of Rhizoctonia on Virginia tobacco cultivars in the major production areas of Argentina.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7151
Mercado Cardenas, Guadalupe E.; Galván, Marta Zulema; Barrera, Viviana A.; Rodriguero, Marcela Silvina; Carmona, Marcelo A.; et al.; Molecular characterization and pathogenicity of Rhizoctonia spp. isolates from a tobacco growing area in northwestern Argentina; Sociedade Brasileira de Fitopatologia; Tropical Plant Pathology; 40; 3; 7-2015; 160-168
1982-5676
1983-2052
url http://hdl.handle.net/11336/7151
identifier_str_mv Mercado Cardenas, Guadalupe E.; Galván, Marta Zulema; Barrera, Viviana A.; Rodriguero, Marcela Silvina; Carmona, Marcelo A.; et al.; Molecular characterization and pathogenicity of Rhizoctonia spp. isolates from a tobacco growing area in northwestern Argentina; Sociedade Brasileira de Fitopatologia; Tropical Plant Pathology; 40; 3; 7-2015; 160-168
1982-5676
1983-2052
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s40858-015-0035-7
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s40858-015-0035-7
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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