Changes in the kinetic properties of the a9a10 hair cell nicotinic receptor increase the level of olivocochlear inhibition in auditory synapses

Autores
Wedemeyer, Carolina; Vattino, Lucas Gabriel; Ballestero, Jimena Andrea; Katz, Eleonora; Elgoyhen, Ana Belen
Año de publicación
2016
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Medial olivocochlear (MOC) fibers innervate the cochlear outer (OHCs) hair cells and also transiently during development, the inner hair cells (IHCs). The MOC-hair cell synapse is cholinergic, inhibitory and is mediated by a9a10 nicotinic receptors (nAChR). In mice bearing a point mutation in the a9 nAChR subunit (Kin), in-vivo cochlear efferent-mediated inhibition is dramatically lengthened and enhanced (Taranda 2009). We now investigated whether there are changes in the short-term plasticity properties (STP) of MOC-hair cells synapses that could account for this enhanced efferent suppression. Evoked synaptic currents were recorded in IHCs and OHCs of isolated mouse organs of Corti. MOC-OHC synapses from Kin mice significantly facilitated upon 20 and 40 Hz stimulation (p2/p1- 20Hz= 2.6; p2/p1- 40Hz= 3.2; n=4-8). The probability of release increased during the trains, whereas the size of the events remained stable suggesting a presynaptic origin. Moreover, stimulation of MOC fibers at 80 Hz for 10 s resulted in postsynaptic responses of longer duration and slower onset times in both OHCs and IHCs from Kin mice (n=8-16). In accordance, preliminary experiments show that efferent inhibition of calcium action potentials is more potent in IHCs from kin mice. We conclude that the alteration in the STP patterns of MOC-hair cell synapses caused by the mutation could contribute to the observed changes in the dynamics of in-vivo cochlear efferent inhibition.
Fil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Vattino, Lucas Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Ballestero, Jimena Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Katz, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; Argentina
2nd Congress of the Federation of Latin-American and Caribbean Societies for Neuroscience; XXXIX Reunião Anual da la Sociedade Brasileira de Neurociências e Comportamento; XII Reunión Anual de la Sociedad Chilena de Neurociencias; XV Jornadas de la Sociedad de Neurociencias del Uruguay; XXXI Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias
Ciudad Autónoma de Buenos Aires
Argentina
Federation of Latin-American and Caribbean Societies for Neuroscience
Sociedad Argentina de Investigación en Neurociencias
Sociedad de Neurociencias del Uruguay
Sociedade Brasileira de Neurociências e Comportamento
Sociedad Chilena de Neurociencias
Materia
EFFERENT INHIBITION
KNOCK IN
ALPHA9 ALPHA10 RECEPTOR
COCHLEA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/236322
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/236322
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Changes in the kinetic properties of the a9a10 hair cell nicotinic receptor increase the level of olivocochlear inhibition in auditory synapsesWedemeyer, CarolinaVattino, Lucas GabrielBallestero, Jimena AndreaKatz, EleonoraElgoyhen, Ana BelenEFFERENT INHIBITIONKNOCK INALPHA9 ALPHA10 RECEPTORCOCHLEAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Medial olivocochlear (MOC) fibers innervate the cochlear outer (OHCs) hair cells and also transiently during development, the inner hair cells (IHCs). The MOC-hair cell synapse is cholinergic, inhibitory and is mediated by a9a10 nicotinic receptors (nAChR). In mice bearing a point mutation in the a9 nAChR subunit (Kin), in-vivo cochlear efferent-mediated inhibition is dramatically lengthened and enhanced (Taranda 2009). We now investigated whether there are changes in the short-term plasticity properties (STP) of MOC-hair cells synapses that could account for this enhanced efferent suppression. Evoked synaptic currents were recorded in IHCs and OHCs of isolated mouse organs of Corti. MOC-OHC synapses from Kin mice significantly facilitated upon 20 and 40 Hz stimulation (p2/p1- 20Hz= 2.6; p2/p1- 40Hz= 3.2; n=4-8). The probability of release increased during the trains, whereas the size of the events remained stable suggesting a presynaptic origin. Moreover, stimulation of MOC fibers at 80 Hz for 10 s resulted in postsynaptic responses of longer duration and slower onset times in both OHCs and IHCs from Kin mice (n=8-16). In accordance, preliminary experiments show that efferent inhibition of calcium action potentials is more potent in IHCs from kin mice. We conclude that the alteration in the STP patterns of MOC-hair cell synapses caused by the mutation could contribute to the observed changes in the dynamics of in-vivo cochlear efferent inhibition.Fil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Vattino, Lucas Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Ballestero, Jimena Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Katz, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; Argentina2nd Congress of the Federation of Latin-American and Caribbean Societies for Neuroscience; XXXIX Reunião Anual da la Sociedade Brasileira de Neurociências e Comportamento; XII Reunión Anual de la Sociedad Chilena de Neurociencias; XV Jornadas de la Sociedad de Neurociencias del Uruguay; XXXI Congreso Anual de la Sociedad Argentina de Investigación en NeurocienciasCiudad Autónoma de Buenos AiresArgentinaFederation of Latin-American and Caribbean Societies for NeuroscienceSociedad Argentina de Investigación en NeurocienciasSociedad de Neurociencias del UruguaySociedade Brasileira de Neurociências e ComportamentoSociedad Chilena de NeurocienciasSociedad Argentina de Investigación en Neurociencias2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/236322Changes in the kinetic properties of the a9a10 hair cell nicotinic receptor increase the level of olivocochlear inhibition in auditory synapses; 2nd Congress of the Federation of Latin-American and Caribbean Societies for Neuroscience; XXXIX Reunião Anual da la Sociedade Brasileira de Neurociências e Comportamento; XII Reunión Anual de la Sociedad Chilena de Neurociencias; XV Jornadas de la Sociedad de Neurociencias del Uruguay; XXXI Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias; Ciudad Autónoma de Buenos Aires; Argentina; 2016; 336-336CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saneurociencias.org.ar/wp-content/uploads/2022/01/FALAN_SAN2016_Buenos-Aires_libro-de-resumenes.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:57Zoai:ri.conicet.gov.ar:11336/236322instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:58.234CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Changes in the kinetic properties of the a9a10 hair cell nicotinic receptor increase the level of olivocochlear inhibition in auditory synapses
title Changes in the kinetic properties of the a9a10 hair cell nicotinic receptor increase the level of olivocochlear inhibition in auditory synapses
spellingShingle Changes in the kinetic properties of the a9a10 hair cell nicotinic receptor increase the level of olivocochlear inhibition in auditory synapses
Wedemeyer, Carolina
EFFERENT INHIBITION
KNOCK IN
ALPHA9 ALPHA10 RECEPTOR
COCHLEA
title_short Changes in the kinetic properties of the a9a10 hair cell nicotinic receptor increase the level of olivocochlear inhibition in auditory synapses
title_full Changes in the kinetic properties of the a9a10 hair cell nicotinic receptor increase the level of olivocochlear inhibition in auditory synapses
title_fullStr Changes in the kinetic properties of the a9a10 hair cell nicotinic receptor increase the level of olivocochlear inhibition in auditory synapses
title_full_unstemmed Changes in the kinetic properties of the a9a10 hair cell nicotinic receptor increase the level of olivocochlear inhibition in auditory synapses
title_sort Changes in the kinetic properties of the a9a10 hair cell nicotinic receptor increase the level of olivocochlear inhibition in auditory synapses
dc.creator.none.fl_str_mv Wedemeyer, Carolina
Vattino, Lucas Gabriel
Ballestero, Jimena Andrea
Katz, Eleonora
Elgoyhen, Ana Belen
author Wedemeyer, Carolina
author_facet Wedemeyer, Carolina
Vattino, Lucas Gabriel
Ballestero, Jimena Andrea
Katz, Eleonora
Elgoyhen, Ana Belen
author_role author
author2 Vattino, Lucas Gabriel
Ballestero, Jimena Andrea
Katz, Eleonora
Elgoyhen, Ana Belen
author2_role author
author
author
author
dc.subject.none.fl_str_mv EFFERENT INHIBITION
KNOCK IN
ALPHA9 ALPHA10 RECEPTOR
COCHLEA
topic EFFERENT INHIBITION
KNOCK IN
ALPHA9 ALPHA10 RECEPTOR
COCHLEA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Medial olivocochlear (MOC) fibers innervate the cochlear outer (OHCs) hair cells and also transiently during development, the inner hair cells (IHCs). The MOC-hair cell synapse is cholinergic, inhibitory and is mediated by a9a10 nicotinic receptors (nAChR). In mice bearing a point mutation in the a9 nAChR subunit (Kin), in-vivo cochlear efferent-mediated inhibition is dramatically lengthened and enhanced (Taranda 2009). We now investigated whether there are changes in the short-term plasticity properties (STP) of MOC-hair cells synapses that could account for this enhanced efferent suppression. Evoked synaptic currents were recorded in IHCs and OHCs of isolated mouse organs of Corti. MOC-OHC synapses from Kin mice significantly facilitated upon 20 and 40 Hz stimulation (p2/p1- 20Hz= 2.6; p2/p1- 40Hz= 3.2; n=4-8). The probability of release increased during the trains, whereas the size of the events remained stable suggesting a presynaptic origin. Moreover, stimulation of MOC fibers at 80 Hz for 10 s resulted in postsynaptic responses of longer duration and slower onset times in both OHCs and IHCs from Kin mice (n=8-16). In accordance, preliminary experiments show that efferent inhibition of calcium action potentials is more potent in IHCs from kin mice. We conclude that the alteration in the STP patterns of MOC-hair cell synapses caused by the mutation could contribute to the observed changes in the dynamics of in-vivo cochlear efferent inhibition.
Fil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Vattino, Lucas Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Ballestero, Jimena Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Katz, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; Argentina
2nd Congress of the Federation of Latin-American and Caribbean Societies for Neuroscience; XXXIX Reunião Anual da la Sociedade Brasileira de Neurociências e Comportamento; XII Reunión Anual de la Sociedad Chilena de Neurociencias; XV Jornadas de la Sociedad de Neurociencias del Uruguay; XXXI Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias
Ciudad Autónoma de Buenos Aires
Argentina
Federation of Latin-American and Caribbean Societies for Neuroscience
Sociedad Argentina de Investigación en Neurociencias
Sociedad de Neurociencias del Uruguay
Sociedade Brasileira de Neurociências e Comportamento
Sociedad Chilena de Neurociencias
description Medial olivocochlear (MOC) fibers innervate the cochlear outer (OHCs) hair cells and also transiently during development, the inner hair cells (IHCs). The MOC-hair cell synapse is cholinergic, inhibitory and is mediated by a9a10 nicotinic receptors (nAChR). In mice bearing a point mutation in the a9 nAChR subunit (Kin), in-vivo cochlear efferent-mediated inhibition is dramatically lengthened and enhanced (Taranda 2009). We now investigated whether there are changes in the short-term plasticity properties (STP) of MOC-hair cells synapses that could account for this enhanced efferent suppression. Evoked synaptic currents were recorded in IHCs and OHCs of isolated mouse organs of Corti. MOC-OHC synapses from Kin mice significantly facilitated upon 20 and 40 Hz stimulation (p2/p1- 20Hz= 2.6; p2/p1- 40Hz= 3.2; n=4-8). The probability of release increased during the trains, whereas the size of the events remained stable suggesting a presynaptic origin. Moreover, stimulation of MOC fibers at 80 Hz for 10 s resulted in postsynaptic responses of longer duration and slower onset times in both OHCs and IHCs from Kin mice (n=8-16). In accordance, preliminary experiments show that efferent inhibition of calcium action potentials is more potent in IHCs from kin mice. We conclude that the alteration in the STP patterns of MOC-hair cell synapses caused by the mutation could contribute to the observed changes in the dynamics of in-vivo cochlear efferent inhibition.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
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Book
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info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
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dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/236322
Changes in the kinetic properties of the a9a10 hair cell nicotinic receptor increase the level of olivocochlear inhibition in auditory synapses; 2nd Congress of the Federation of Latin-American and Caribbean Societies for Neuroscience; XXXIX Reunião Anual da la Sociedade Brasileira de Neurociências e Comportamento; XII Reunión Anual de la Sociedad Chilena de Neurociencias; XV Jornadas de la Sociedad de Neurociencias del Uruguay; XXXI Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias; Ciudad Autónoma de Buenos Aires; Argentina; 2016; 336-336
CONICET Digital
CONICET
url http://hdl.handle.net/11336/236322
identifier_str_mv Changes in the kinetic properties of the a9a10 hair cell nicotinic receptor increase the level of olivocochlear inhibition in auditory synapses; 2nd Congress of the Federation of Latin-American and Caribbean Societies for Neuroscience; XXXIX Reunião Anual da la Sociedade Brasileira de Neurociências e Comportamento; XII Reunión Anual de la Sociedad Chilena de Neurociencias; XV Jornadas de la Sociedad de Neurociencias del Uruguay; XXXI Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias; Ciudad Autónoma de Buenos Aires; Argentina; 2016; 336-336
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saneurociencias.org.ar/wp-content/uploads/2022/01/FALAN_SAN2016_Buenos-Aires_libro-de-resumenes.pdf
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencias
publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencias
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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