First PCR-free electrochemical bioplatform for the detection of mustard Sin a 1 protein as a potential “hidden” food allergen

Autores
Gamella, Maria; Laza Correa, Anabel; Parrón Ballesteros, Jorge; Bueno, Cristina; Ruiz-Valdepeñas Montiel, Víctor; Pedrero, María; Bertolino, Franco Adrián; Pingarrón, José M.; Villalba, Mayte; Campuzano, Susana
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A disposable electrochemical PCR-free biosensor for the selective detection of a fragment encoding the protein Sin a 1, a 2S albumin considered a diagnostic marker for sensitization to mustard, is reported. The methodology is based on the formation of DNA/RNA heterohybrids by sandwich hybridization of a specific fragment of the Sin a 1 allergen coding sequence with appropriately designed RNA probes. Labeling with commercial antibodies specific to the heteroduplexes and secondary antibodies conjugated with horseradish peroxidase (HRP) was carried out onto the surface of magnetic beads (MBs). Amperometric transduction was undertaken on screen-printed electrodes using H2O2 as enzyme substrate and hydroquinone (HQ) a redox mediator. The electrochemical biosensor allows the simple and fast detection (75 min) of Sin a 1 reaching a limit of detection of 3 pM. The bioplatform was successfully applied to the analysis of the targeted Sin a 1 gene specific region using just 50 ng of non-fragmented denatured genomic DNA extracted from yellow mustard seeds.
Fil: Gamella, Maria. Universidad Complutense de Madrid; España
Fil: Laza Correa, Anabel. Universidad Complutense de Madrid; España. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Parrón Ballesteros, Jorge. Universidad Complutense de Madrid; España
Fil: Bueno, Cristina. Universidad Complutense de Madrid; España
Fil: Ruiz-Valdepeñas Montiel, Víctor. Universidad Complutense de Madrid; España
Fil: Pedrero, María. Universidad Complutense de Madrid; España
Fil: Bertolino, Franco Adrián. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Pingarrón, José M.. Universidad Complutense de Madrid; España
Fil: Villalba, Mayte. Universidad Complutense de Madrid; España
Fil: Campuzano, Susana. Universidad Complutense de Madrid; España
Materia
ELECTROCHEMICAL BIOPLATFORM
FOOD ALLERGEN
MUSTARD
PCR-FREE
SIN A 1 GENE
Nivel de accesibilidad
acceso embargado
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/201609
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/201609
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling First PCR-free electrochemical bioplatform for the detection of mustard Sin a 1 protein as a potential “hidden” food allergenGamella, MariaLaza Correa, AnabelParrón Ballesteros, JorgeBueno, CristinaRuiz-Valdepeñas Montiel, VíctorPedrero, MaríaBertolino, Franco AdriánPingarrón, José M.Villalba, MayteCampuzano, SusanaELECTROCHEMICAL BIOPLATFORMFOOD ALLERGENMUSTARDPCR-FREESIN A 1 GENEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A disposable electrochemical PCR-free biosensor for the selective detection of a fragment encoding the protein Sin a 1, a 2S albumin considered a diagnostic marker for sensitization to mustard, is reported. The methodology is based on the formation of DNA/RNA heterohybrids by sandwich hybridization of a specific fragment of the Sin a 1 allergen coding sequence with appropriately designed RNA probes. Labeling with commercial antibodies specific to the heteroduplexes and secondary antibodies conjugated with horseradish peroxidase (HRP) was carried out onto the surface of magnetic beads (MBs). Amperometric transduction was undertaken on screen-printed electrodes using H2O2 as enzyme substrate and hydroquinone (HQ) a redox mediator. The electrochemical biosensor allows the simple and fast detection (75 min) of Sin a 1 reaching a limit of detection of 3 pM. The bioplatform was successfully applied to the analysis of the targeted Sin a 1 gene specific region using just 50 ng of non-fragmented denatured genomic DNA extracted from yellow mustard seeds.Fil: Gamella, Maria. Universidad Complutense de Madrid; EspañaFil: Laza Correa, Anabel. Universidad Complutense de Madrid; España. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Parrón Ballesteros, Jorge. Universidad Complutense de Madrid; EspañaFil: Bueno, Cristina. Universidad Complutense de Madrid; EspañaFil: Ruiz-Valdepeñas Montiel, Víctor. Universidad Complutense de Madrid; EspañaFil: Pedrero, María. Universidad Complutense de Madrid; EspañaFil: Bertolino, Franco Adrián. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Pingarrón, José M.. Universidad Complutense de Madrid; EspañaFil: Villalba, Mayte. Universidad Complutense de Madrid; EspañaFil: Campuzano, Susana. Universidad Complutense de Madrid; EspañaElsevier Science SA2022-12info:eu-repo/date/embargoEnd/2023-06-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/201609Gamella, Maria; Laza Correa, Anabel; Parrón Ballesteros, Jorge; Bueno, Cristina; Ruiz-Valdepeñas Montiel, Víctor; et al.; First PCR-free electrochemical bioplatform for the detection of mustard Sin a 1 protein as a potential “hidden” food allergen; Elsevier Science SA; Bioelectrochemistry; 150; 108357; 12-2022; 1-81567-5394CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bioelechem.2022.108357info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1567539422003085info:eu-repo/semantics/embargoedAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:45:50Zoai:ri.conicet.gov.ar:11336/201609instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:45:51.197CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First PCR-free electrochemical bioplatform for the detection of mustard Sin a 1 protein as a potential “hidden” food allergen
title First PCR-free electrochemical bioplatform for the detection of mustard Sin a 1 protein as a potential “hidden” food allergen
spellingShingle First PCR-free electrochemical bioplatform for the detection of mustard Sin a 1 protein as a potential “hidden” food allergen
Gamella, Maria
ELECTROCHEMICAL BIOPLATFORM
FOOD ALLERGEN
MUSTARD
PCR-FREE
SIN A 1 GENE
title_short First PCR-free electrochemical bioplatform for the detection of mustard Sin a 1 protein as a potential “hidden” food allergen
title_full First PCR-free electrochemical bioplatform for the detection of mustard Sin a 1 protein as a potential “hidden” food allergen
title_fullStr First PCR-free electrochemical bioplatform for the detection of mustard Sin a 1 protein as a potential “hidden” food allergen
title_full_unstemmed First PCR-free electrochemical bioplatform for the detection of mustard Sin a 1 protein as a potential “hidden” food allergen
title_sort First PCR-free electrochemical bioplatform for the detection of mustard Sin a 1 protein as a potential “hidden” food allergen
dc.creator.none.fl_str_mv Gamella, Maria
Laza Correa, Anabel
Parrón Ballesteros, Jorge
Bueno, Cristina
Ruiz-Valdepeñas Montiel, Víctor
Pedrero, María
Bertolino, Franco Adrián
Pingarrón, José M.
Villalba, Mayte
Campuzano, Susana
author Gamella, Maria
author_facet Gamella, Maria
Laza Correa, Anabel
Parrón Ballesteros, Jorge
Bueno, Cristina
Ruiz-Valdepeñas Montiel, Víctor
Pedrero, María
Bertolino, Franco Adrián
Pingarrón, José M.
Villalba, Mayte
Campuzano, Susana
author_role author
author2 Laza Correa, Anabel
Parrón Ballesteros, Jorge
Bueno, Cristina
Ruiz-Valdepeñas Montiel, Víctor
Pedrero, María
Bertolino, Franco Adrián
Pingarrón, José M.
Villalba, Mayte
Campuzano, Susana
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ELECTROCHEMICAL BIOPLATFORM
FOOD ALLERGEN
MUSTARD
PCR-FREE
SIN A 1 GENE
topic ELECTROCHEMICAL BIOPLATFORM
FOOD ALLERGEN
MUSTARD
PCR-FREE
SIN A 1 GENE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A disposable electrochemical PCR-free biosensor for the selective detection of a fragment encoding the protein Sin a 1, a 2S albumin considered a diagnostic marker for sensitization to mustard, is reported. The methodology is based on the formation of DNA/RNA heterohybrids by sandwich hybridization of a specific fragment of the Sin a 1 allergen coding sequence with appropriately designed RNA probes. Labeling with commercial antibodies specific to the heteroduplexes and secondary antibodies conjugated with horseradish peroxidase (HRP) was carried out onto the surface of magnetic beads (MBs). Amperometric transduction was undertaken on screen-printed electrodes using H2O2 as enzyme substrate and hydroquinone (HQ) a redox mediator. The electrochemical biosensor allows the simple and fast detection (75 min) of Sin a 1 reaching a limit of detection of 3 pM. The bioplatform was successfully applied to the analysis of the targeted Sin a 1 gene specific region using just 50 ng of non-fragmented denatured genomic DNA extracted from yellow mustard seeds.
Fil: Gamella, Maria. Universidad Complutense de Madrid; España
Fil: Laza Correa, Anabel. Universidad Complutense de Madrid; España. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Parrón Ballesteros, Jorge. Universidad Complutense de Madrid; España
Fil: Bueno, Cristina. Universidad Complutense de Madrid; España
Fil: Ruiz-Valdepeñas Montiel, Víctor. Universidad Complutense de Madrid; España
Fil: Pedrero, María. Universidad Complutense de Madrid; España
Fil: Bertolino, Franco Adrián. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Pingarrón, José M.. Universidad Complutense de Madrid; España
Fil: Villalba, Mayte. Universidad Complutense de Madrid; España
Fil: Campuzano, Susana. Universidad Complutense de Madrid; España
description A disposable electrochemical PCR-free biosensor for the selective detection of a fragment encoding the protein Sin a 1, a 2S albumin considered a diagnostic marker for sensitization to mustard, is reported. The methodology is based on the formation of DNA/RNA heterohybrids by sandwich hybridization of a specific fragment of the Sin a 1 allergen coding sequence with appropriately designed RNA probes. Labeling with commercial antibodies specific to the heteroduplexes and secondary antibodies conjugated with horseradish peroxidase (HRP) was carried out onto the surface of magnetic beads (MBs). Amperometric transduction was undertaken on screen-printed electrodes using H2O2 as enzyme substrate and hydroquinone (HQ) a redox mediator. The electrochemical biosensor allows the simple and fast detection (75 min) of Sin a 1 reaching a limit of detection of 3 pM. The bioplatform was successfully applied to the analysis of the targeted Sin a 1 gene specific region using just 50 ng of non-fragmented denatured genomic DNA extracted from yellow mustard seeds.
publishDate 2022
dc.date.none.fl_str_mv 2022-12
info:eu-repo/date/embargoEnd/2023-06-21
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/201609
Gamella, Maria; Laza Correa, Anabel; Parrón Ballesteros, Jorge; Bueno, Cristina; Ruiz-Valdepeñas Montiel, Víctor; et al.; First PCR-free electrochemical bioplatform for the detection of mustard Sin a 1 protein as a potential “hidden” food allergen; Elsevier Science SA; Bioelectrochemistry; 150; 108357; 12-2022; 1-8
1567-5394
CONICET Digital
CONICET
url http://hdl.handle.net/11336/201609
identifier_str_mv Gamella, Maria; Laza Correa, Anabel; Parrón Ballesteros, Jorge; Bueno, Cristina; Ruiz-Valdepeñas Montiel, Víctor; et al.; First PCR-free electrochemical bioplatform for the detection of mustard Sin a 1 protein as a potential “hidden” food allergen; Elsevier Science SA; Bioelectrochemistry; 150; 108357; 12-2022; 1-8
1567-5394
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bioelechem.2022.108357
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1567539422003085
dc.rights.none.fl_str_mv info:eu-repo/semantics/embargoedAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv embargoedAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science SA
publisher.none.fl_str_mv Elsevier Science SA
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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