Simultaneous determination of cyclophosphamide and carboxyethylphosphoramide mustard in human plasma using online extraction and electrospray tandem mass spectrometry (HTLC-ESI-MS/...

Autores
Bai, Feng; Fraga, Charles H.; Tagen, Michael; Schaiquevich, Paula Susana; Hagedorn, Nikolaus; Stewart, Clinton F.
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A rapid and selective method for simultaneous determination of cyclophosphamide and its metabolite carboxyethylphosphoramide mustard (CEPM) was developed using online sample preparation and separation with tandem mass spectrometric detection. Diluted plasma was injected onto an extraction column (Cyclone MAX 0.5 mm × 50 mm, >30 μm), the sample matrix was washed with an aqueous solution, and retained analytes were transferred to an analytical column (Gemini 3 μm C18 110A, 100 mm × 2.0 mm) using a gradient mobile phase prior to detection by MS/MS. Analytes were detected in an API-3000 LC-MS/MS system using positive multiple-reaction monitoring mode (m/z 261/140 and 293/221 for CTX and CEPM, respectively). Online extraction recoveries were 76% and 72% for cyclophosphamide and CEPM. Within-day and between-day variabilities were <3.0%, and accuracies were between -6.9% and 5.2%. This method has been used to measure plasma cyclophosphamide and CEPM concentrations in an ongoing Phase II study in children with newly diagnosed medulloblastoma.
Fil: Bai, Feng. St. Jude Children's Research Hospital; Estados Unidos
Fil: Fraga, Charles H.. St. Jude Children's Research Hospital; Estados Unidos
Fil: Tagen, Michael. St. Jude Children's Research Hospital; Estados Unidos
Fil: Schaiquevich, Paula Susana. St. Jude Children's Research Hospital; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hagedorn, Nikolaus. St. Jude Children's Research Hospital; Estados Unidos
Fil: Stewart, Clinton F.. University Of Tennessee Health Science Center; Estados Unidos. St. Jude Children's Research Hospital; Estados Unidos
Materia
CARBOXYETHYLPHOSPHORAMIDE MUSTARD
CYCLOPHOSPHAMIDE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/139029
Acceder
id CONICETDig_badd5a3e86e9709f3c49a1d6920cd62f
oai_identifier_str oai:ri.conicet.gov.ar:11336/139029
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Simultaneous determination of cyclophosphamide and carboxyethylphosphoramide mustard in human plasma using online extraction and electrospray tandem mass spectrometry (HTLC-ESI-MS/MS)Bai, FengFraga, Charles H.Tagen, MichaelSchaiquevich, Paula SusanaHagedorn, NikolausStewart, Clinton F.CARBOXYETHYLPHOSPHORAMIDE MUSTARDCYCLOPHOSPHAMIDEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3A rapid and selective method for simultaneous determination of cyclophosphamide and its metabolite carboxyethylphosphoramide mustard (CEPM) was developed using online sample preparation and separation with tandem mass spectrometric detection. Diluted plasma was injected onto an extraction column (Cyclone MAX 0.5 mm × 50 mm, >30 μm), the sample matrix was washed with an aqueous solution, and retained analytes were transferred to an analytical column (Gemini 3 μm C18 110A, 100 mm × 2.0 mm) using a gradient mobile phase prior to detection by MS/MS. Analytes were detected in an API-3000 LC-MS/MS system using positive multiple-reaction monitoring mode (m/z 261/140 and 293/221 for CTX and CEPM, respectively). Online extraction recoveries were 76% and 72% for cyclophosphamide and CEPM. Within-day and between-day variabilities were <3.0%, and accuracies were between -6.9% and 5.2%. This method has been used to measure plasma cyclophosphamide and CEPM concentrations in an ongoing Phase II study in children with newly diagnosed medulloblastoma.Fil: Bai, Feng. St. Jude Children's Research Hospital; Estados UnidosFil: Fraga, Charles H.. St. Jude Children's Research Hospital; Estados UnidosFil: Tagen, Michael. St. Jude Children's Research Hospital; Estados UnidosFil: Schaiquevich, Paula Susana. St. Jude Children's Research Hospital; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hagedorn, Nikolaus. St. Jude Children's Research Hospital; Estados UnidosFil: Stewart, Clinton F.. University Of Tennessee Health Science Center; Estados Unidos. St. Jude Children's Research Hospital; Estados UnidosElsevier Science2009-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/139029Bai, Feng; Fraga, Charles H.; Tagen, Michael; Schaiquevich, Paula Susana; Hagedorn, Nikolaus; et al.; Simultaneous determination of cyclophosphamide and carboxyethylphosphoramide mustard in human plasma using online extraction and electrospray tandem mass spectrometry (HTLC-ESI-MS/MS); Elsevier Science; Journal of Chromatography B; 877; 18-19; 6-2009; 1709-17151570-0232CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S1570023209002876info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jchromb.2009.04.025info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:10:42Zoai:ri.conicet.gov.ar:11336/139029instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:10:42.825CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Simultaneous determination of cyclophosphamide and carboxyethylphosphoramide mustard in human plasma using online extraction and electrospray tandem mass spectrometry (HTLC-ESI-MS/MS)
title Simultaneous determination of cyclophosphamide and carboxyethylphosphoramide mustard in human plasma using online extraction and electrospray tandem mass spectrometry (HTLC-ESI-MS/MS)
spellingShingle Simultaneous determination of cyclophosphamide and carboxyethylphosphoramide mustard in human plasma using online extraction and electrospray tandem mass spectrometry (HTLC-ESI-MS/MS)
Bai, Feng
CARBOXYETHYLPHOSPHORAMIDE MUSTARD
CYCLOPHOSPHAMIDE
title_short Simultaneous determination of cyclophosphamide and carboxyethylphosphoramide mustard in human plasma using online extraction and electrospray tandem mass spectrometry (HTLC-ESI-MS/MS)
title_full Simultaneous determination of cyclophosphamide and carboxyethylphosphoramide mustard in human plasma using online extraction and electrospray tandem mass spectrometry (HTLC-ESI-MS/MS)
title_fullStr Simultaneous determination of cyclophosphamide and carboxyethylphosphoramide mustard in human plasma using online extraction and electrospray tandem mass spectrometry (HTLC-ESI-MS/MS)
title_full_unstemmed Simultaneous determination of cyclophosphamide and carboxyethylphosphoramide mustard in human plasma using online extraction and electrospray tandem mass spectrometry (HTLC-ESI-MS/MS)
title_sort Simultaneous determination of cyclophosphamide and carboxyethylphosphoramide mustard in human plasma using online extraction and electrospray tandem mass spectrometry (HTLC-ESI-MS/MS)
dc.creator.none.fl_str_mv Bai, Feng
Fraga, Charles H.
Tagen, Michael
Schaiquevich, Paula Susana
Hagedorn, Nikolaus
Stewart, Clinton F.
author Bai, Feng
author_facet Bai, Feng
Fraga, Charles H.
Tagen, Michael
Schaiquevich, Paula Susana
Hagedorn, Nikolaus
Stewart, Clinton F.
author_role author
author2 Fraga, Charles H.
Tagen, Michael
Schaiquevich, Paula Susana
Hagedorn, Nikolaus
Stewart, Clinton F.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CARBOXYETHYLPHOSPHORAMIDE MUSTARD
CYCLOPHOSPHAMIDE
topic CARBOXYETHYLPHOSPHORAMIDE MUSTARD
CYCLOPHOSPHAMIDE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv A rapid and selective method for simultaneous determination of cyclophosphamide and its metabolite carboxyethylphosphoramide mustard (CEPM) was developed using online sample preparation and separation with tandem mass spectrometric detection. Diluted plasma was injected onto an extraction column (Cyclone MAX 0.5 mm × 50 mm, >30 μm), the sample matrix was washed with an aqueous solution, and retained analytes were transferred to an analytical column (Gemini 3 μm C18 110A, 100 mm × 2.0 mm) using a gradient mobile phase prior to detection by MS/MS. Analytes were detected in an API-3000 LC-MS/MS system using positive multiple-reaction monitoring mode (m/z 261/140 and 293/221 for CTX and CEPM, respectively). Online extraction recoveries were 76% and 72% for cyclophosphamide and CEPM. Within-day and between-day variabilities were <3.0%, and accuracies were between -6.9% and 5.2%. This method has been used to measure plasma cyclophosphamide and CEPM concentrations in an ongoing Phase II study in children with newly diagnosed medulloblastoma.
Fil: Bai, Feng. St. Jude Children's Research Hospital; Estados Unidos
Fil: Fraga, Charles H.. St. Jude Children's Research Hospital; Estados Unidos
Fil: Tagen, Michael. St. Jude Children's Research Hospital; Estados Unidos
Fil: Schaiquevich, Paula Susana. St. Jude Children's Research Hospital; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hagedorn, Nikolaus. St. Jude Children's Research Hospital; Estados Unidos
Fil: Stewart, Clinton F.. University Of Tennessee Health Science Center; Estados Unidos. St. Jude Children's Research Hospital; Estados Unidos
description A rapid and selective method for simultaneous determination of cyclophosphamide and its metabolite carboxyethylphosphoramide mustard (CEPM) was developed using online sample preparation and separation with tandem mass spectrometric detection. Diluted plasma was injected onto an extraction column (Cyclone MAX 0.5 mm × 50 mm, >30 μm), the sample matrix was washed with an aqueous solution, and retained analytes were transferred to an analytical column (Gemini 3 μm C18 110A, 100 mm × 2.0 mm) using a gradient mobile phase prior to detection by MS/MS. Analytes were detected in an API-3000 LC-MS/MS system using positive multiple-reaction monitoring mode (m/z 261/140 and 293/221 for CTX and CEPM, respectively). Online extraction recoveries were 76% and 72% for cyclophosphamide and CEPM. Within-day and between-day variabilities were <3.0%, and accuracies were between -6.9% and 5.2%. This method has been used to measure plasma cyclophosphamide and CEPM concentrations in an ongoing Phase II study in children with newly diagnosed medulloblastoma.
publishDate 2009
dc.date.none.fl_str_mv 2009-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/139029
Bai, Feng; Fraga, Charles H.; Tagen, Michael; Schaiquevich, Paula Susana; Hagedorn, Nikolaus; et al.; Simultaneous determination of cyclophosphamide and carboxyethylphosphoramide mustard in human plasma using online extraction and electrospray tandem mass spectrometry (HTLC-ESI-MS/MS); Elsevier Science; Journal of Chromatography B; 877; 18-19; 6-2009; 1709-1715
1570-0232
CONICET Digital
CONICET
url http://hdl.handle.net/11336/139029
identifier_str_mv Bai, Feng; Fraga, Charles H.; Tagen, Michael; Schaiquevich, Paula Susana; Hagedorn, Nikolaus; et al.; Simultaneous determination of cyclophosphamide and carboxyethylphosphoramide mustard in human plasma using online extraction and electrospray tandem mass spectrometry (HTLC-ESI-MS/MS); Elsevier Science; Journal of Chromatography B; 877; 18-19; 6-2009; 1709-1715
1570-0232
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S1570023209002876
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jchromb.2009.04.025
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782494588796928
score 12.982451

Publicaciones similares