One-step enzyme-free dual electrochemical immunosensor for histidine-rich protein 2 determination

Autores
Dip Gandarilla, Ariamna María; Regiart, Daniel Matias Gaston; Bertotti, Mauro; Correa Glória, Juliane; Morais Mariuba, Luís André; Brito, Walter Ricardo
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the present work, we describe a novel one-step enzyme-free dual electrochemical immunosensor for the determination of histidine-rich protein 2 (Ag-PfHRP2), a specific malaria biomarker. A gold electrode (GE) was functionalized with thePfHRP2 antibody (Ab-PfHRP2) using dihexadecyl phosphate (DHP) polymer as an immobilization platform. The Ab-PfHRP2/DHP/GE sensor was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, Fourier-transform infrared spectroscopy, scanning electron microscopy, and atomic force microscopy. The developed immunosensor was employed for indirect Ag-PfHRP2 determination by differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). The linear range was 10-400 ng mL−1and 10-500 ng mL−1for EIS and DPV, while the limit of detection was 3.3 ng mL−1and 2.8 ng mL−1, respectively. The electrochemical immunosensor was successfully applied for Ag-PfHRP2 determination in human serum samples. Its performance was compared with an ELISA test, and good correspondence was achieved. The coefficients of intra- and inter-assay variations were less than 5%. The electrochemical immunosensor is a useful and straightforward tool forin situmalaria biomarker determination.
Fil: Dip Gandarilla, Ariamna María. Universidad Federal del Amazonas.; Brasil
Fil: Regiart, Daniel Matias Gaston. Universidade de Sao Paulo; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Bertotti, Mauro. Universidade de Sao Paulo; Brasil
Fil: Correa Glória, Juliane. Fundación Oswaldo Cruz; Brasil
Fil: Morais Mariuba, Luís André. Fundación Oswaldo Cruz; Brasil
Fil: Brito, Walter Ricardo. Universidad Federal del Amazonas.; Brasil
Materia
enzyme-free
histidine-rich protein 2
immunosensor
electrochemical
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/201497
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/201497
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling One-step enzyme-free dual electrochemical immunosensor for histidine-rich protein 2 determinationDip Gandarilla, Ariamna MaríaRegiart, Daniel Matias GastonBertotti, MauroCorrea Glória, JulianeMorais Mariuba, Luís AndréBrito, Walter Ricardoenzyme-freehistidine-rich protein 2immunosensorelectrochemicalhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In the present work, we describe a novel one-step enzyme-free dual electrochemical immunosensor for the determination of histidine-rich protein 2 (Ag-PfHRP2), a specific malaria biomarker. A gold electrode (GE) was functionalized with thePfHRP2 antibody (Ab-PfHRP2) using dihexadecyl phosphate (DHP) polymer as an immobilization platform. The Ab-PfHRP2/DHP/GE sensor was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, Fourier-transform infrared spectroscopy, scanning electron microscopy, and atomic force microscopy. The developed immunosensor was employed for indirect Ag-PfHRP2 determination by differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). The linear range was 10-400 ng mL−1and 10-500 ng mL−1for EIS and DPV, while the limit of detection was 3.3 ng mL−1and 2.8 ng mL−1, respectively. The electrochemical immunosensor was successfully applied for Ag-PfHRP2 determination in human serum samples. Its performance was compared with an ELISA test, and good correspondence was achieved. The coefficients of intra- and inter-assay variations were less than 5%. The electrochemical immunosensor is a useful and straightforward tool forin situmalaria biomarker determination.Fil: Dip Gandarilla, Ariamna María. Universidad Federal del Amazonas.; BrasilFil: Regiart, Daniel Matias Gaston. Universidade de Sao Paulo; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Bertotti, Mauro. Universidade de Sao Paulo; BrasilFil: Correa Glória, Juliane. Fundación Oswaldo Cruz; BrasilFil: Morais Mariuba, Luís André. Fundación Oswaldo Cruz; BrasilFil: Brito, Walter Ricardo. Universidad Federal del Amazonas.; BrasilRoyal Society of Chemistry2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/201497Dip Gandarilla, Ariamna María; Regiart, Daniel Matias Gaston; Bertotti, Mauro; Correa Glória, Juliane; Morais Mariuba, Luís André; et al.; One-step enzyme-free dual electrochemical immunosensor for histidine-rich protein 2 determination; Royal Society of Chemistry; RSC Advances; 11; 1; 12-2020; 408-4152046-2069CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1039/d0ra08729ginfo:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2021/RA/D0RA08729Ginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:37Zoai:ri.conicet.gov.ar:11336/201497instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:37.819CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv One-step enzyme-free dual electrochemical immunosensor for histidine-rich protein 2 determination
title One-step enzyme-free dual electrochemical immunosensor for histidine-rich protein 2 determination
spellingShingle One-step enzyme-free dual electrochemical immunosensor for histidine-rich protein 2 determination
Dip Gandarilla, Ariamna María
enzyme-free
histidine-rich protein 2
immunosensor
electrochemical
title_short One-step enzyme-free dual electrochemical immunosensor for histidine-rich protein 2 determination
title_full One-step enzyme-free dual electrochemical immunosensor for histidine-rich protein 2 determination
title_fullStr One-step enzyme-free dual electrochemical immunosensor for histidine-rich protein 2 determination
title_full_unstemmed One-step enzyme-free dual electrochemical immunosensor for histidine-rich protein 2 determination
title_sort One-step enzyme-free dual electrochemical immunosensor for histidine-rich protein 2 determination
dc.creator.none.fl_str_mv Dip Gandarilla, Ariamna María
Regiart, Daniel Matias Gaston
Bertotti, Mauro
Correa Glória, Juliane
Morais Mariuba, Luís André
Brito, Walter Ricardo
author Dip Gandarilla, Ariamna María
author_facet Dip Gandarilla, Ariamna María
Regiart, Daniel Matias Gaston
Bertotti, Mauro
Correa Glória, Juliane
Morais Mariuba, Luís André
Brito, Walter Ricardo
author_role author
author2 Regiart, Daniel Matias Gaston
Bertotti, Mauro
Correa Glória, Juliane
Morais Mariuba, Luís André
Brito, Walter Ricardo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv enzyme-free
histidine-rich protein 2
immunosensor
electrochemical
topic enzyme-free
histidine-rich protein 2
immunosensor
electrochemical
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In the present work, we describe a novel one-step enzyme-free dual electrochemical immunosensor for the determination of histidine-rich protein 2 (Ag-PfHRP2), a specific malaria biomarker. A gold electrode (GE) was functionalized with thePfHRP2 antibody (Ab-PfHRP2) using dihexadecyl phosphate (DHP) polymer as an immobilization platform. The Ab-PfHRP2/DHP/GE sensor was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, Fourier-transform infrared spectroscopy, scanning electron microscopy, and atomic force microscopy. The developed immunosensor was employed for indirect Ag-PfHRP2 determination by differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). The linear range was 10-400 ng mL−1and 10-500 ng mL−1for EIS and DPV, while the limit of detection was 3.3 ng mL−1and 2.8 ng mL−1, respectively. The electrochemical immunosensor was successfully applied for Ag-PfHRP2 determination in human serum samples. Its performance was compared with an ELISA test, and good correspondence was achieved. The coefficients of intra- and inter-assay variations were less than 5%. The electrochemical immunosensor is a useful and straightforward tool forin situmalaria biomarker determination.
Fil: Dip Gandarilla, Ariamna María. Universidad Federal del Amazonas.; Brasil
Fil: Regiart, Daniel Matias Gaston. Universidade de Sao Paulo; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Bertotti, Mauro. Universidade de Sao Paulo; Brasil
Fil: Correa Glória, Juliane. Fundación Oswaldo Cruz; Brasil
Fil: Morais Mariuba, Luís André. Fundación Oswaldo Cruz; Brasil
Fil: Brito, Walter Ricardo. Universidad Federal del Amazonas.; Brasil
description In the present work, we describe a novel one-step enzyme-free dual electrochemical immunosensor for the determination of histidine-rich protein 2 (Ag-PfHRP2), a specific malaria biomarker. A gold electrode (GE) was functionalized with thePfHRP2 antibody (Ab-PfHRP2) using dihexadecyl phosphate (DHP) polymer as an immobilization platform. The Ab-PfHRP2/DHP/GE sensor was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, Fourier-transform infrared spectroscopy, scanning electron microscopy, and atomic force microscopy. The developed immunosensor was employed for indirect Ag-PfHRP2 determination by differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). The linear range was 10-400 ng mL−1and 10-500 ng mL−1for EIS and DPV, while the limit of detection was 3.3 ng mL−1and 2.8 ng mL−1, respectively. The electrochemical immunosensor was successfully applied for Ag-PfHRP2 determination in human serum samples. Its performance was compared with an ELISA test, and good correspondence was achieved. The coefficients of intra- and inter-assay variations were less than 5%. The electrochemical immunosensor is a useful and straightforward tool forin situmalaria biomarker determination.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/201497
Dip Gandarilla, Ariamna María; Regiart, Daniel Matias Gaston; Bertotti, Mauro; Correa Glória, Juliane; Morais Mariuba, Luís André; et al.; One-step enzyme-free dual electrochemical immunosensor for histidine-rich protein 2 determination; Royal Society of Chemistry; RSC Advances; 11; 1; 12-2020; 408-415
2046-2069
CONICET Digital
CONICET
url http://hdl.handle.net/11336/201497
identifier_str_mv Dip Gandarilla, Ariamna María; Regiart, Daniel Matias Gaston; Bertotti, Mauro; Correa Glória, Juliane; Morais Mariuba, Luís André; et al.; One-step enzyme-free dual electrochemical immunosensor for histidine-rich protein 2 determination; Royal Society of Chemistry; RSC Advances; 11; 1; 12-2020; 408-415
2046-2069
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1039/d0ra08729g
info:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2021/RA/D0RA08729G
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268871762903040
score 13.13397

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