Cryopreservation of seeds and in vitro cultured protocorms of Oncidium bifolium sims. (Orchidaceae) by encapsulation-dehydration

Autores
Flachsland, Eduardo Alberto; Terada, Graciela; Scocchi, A.; Rey de Badaró, Hebe Yolanda; Mroginski, Luis Amado; Engelmann, F.
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Encapsulation-dehydration was employed for cryopreserving seeds and in vitro-cultured protocorms of Oncidium bifolium. Freshly harvested seeds, 120 days after pollination, were encapsulated in beads containing ½ MS medium with 3% sucrose and 3% calcium alginate and subsequently pretreated in agitated (80 rpm) liquid medium supplemented with 0.15 M sucrose (24 h) followed by 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h). The beads with seeds were dehydrated with silica gel for 5 h to 19.2% moisture content and immersed in liquid nitrogen for 1 h, thawed at 30 °C for 2 min, post-treated using the same series of liquid media [0.5 M sucrose (24 h), 0.25 M sucrose (48 h), 0.15 M sucrose (24 h)], and recultured on ½ MS medium with 0.1M sucrose and 0.7% agar. As much as 4.8% of the cryopreserved seeds produced complete plants. In-vitro cultured protocorms were successfully cryopreserved following the same procedure, allowing 11.3% of them to produce plants.
Fil: Flachsland, Eduardo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Terada, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Scocchi, A.. Universidad Nacional del Nordeste; Argentina
Fil: Rey de Badaró, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Engelmann, F.. International Plant Genetic Resources Institute ; Italia
Materia
Cryopreservation
Encapsulation-Dehydration
Oncidium Bifolium
Orchidaceae
Protocorms
Seeds
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41003
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/41003
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cryopreservation of seeds and in vitro cultured protocorms of Oncidium bifolium sims. (Orchidaceae) by encapsulation-dehydrationFlachsland, Eduardo AlbertoTerada, GracielaScocchi, A.Rey de Badaró, Hebe YolandaMroginski, Luis AmadoEngelmann, F.CryopreservationEncapsulation-DehydrationOncidium BifoliumOrchidaceaeProtocormsSeedshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Encapsulation-dehydration was employed for cryopreserving seeds and in vitro-cultured protocorms of Oncidium bifolium. Freshly harvested seeds, 120 days after pollination, were encapsulated in beads containing ½ MS medium with 3% sucrose and 3% calcium alginate and subsequently pretreated in agitated (80 rpm) liquid medium supplemented with 0.15 M sucrose (24 h) followed by 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h). The beads with seeds were dehydrated with silica gel for 5 h to 19.2% moisture content and immersed in liquid nitrogen for 1 h, thawed at 30 °C for 2 min, post-treated using the same series of liquid media [0.5 M sucrose (24 h), 0.25 M sucrose (48 h), 0.15 M sucrose (24 h)], and recultured on ½ MS medium with 0.1M sucrose and 0.7% agar. As much as 4.8% of the cryopreserved seeds produced complete plants. In-vitro cultured protocorms were successfully cryopreserved following the same procedure, allowing 11.3% of them to produce plants.Fil: Flachsland, Eduardo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Terada, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Scocchi, A.. Universidad Nacional del Nordeste; ArgentinaFil: Rey de Badaró, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Engelmann, F.. International Plant Genetic Resources Institute ; ItaliaCryo Letters2006-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41003Flachsland, Eduardo Alberto; Terada, Graciela; Scocchi, A.; Rey de Badaró, Hebe Yolanda; Mroginski, Luis Amado; et al.; Cryopreservation of seeds and in vitro cultured protocorms of Oncidium bifolium sims. (Orchidaceae) by encapsulation-dehydration; Cryo Letters; Cryo-letters; 27; 4; 12-2006; 235-2420143-2044CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/contentone/cryo/cryo/2006/00000027/00000004/art00005#info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:18:27Zoai:ri.conicet.gov.ar:11336/41003instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:18:27.555CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cryopreservation of seeds and in vitro cultured protocorms of Oncidium bifolium sims. (Orchidaceae) by encapsulation-dehydration
title Cryopreservation of seeds and in vitro cultured protocorms of Oncidium bifolium sims. (Orchidaceae) by encapsulation-dehydration
spellingShingle Cryopreservation of seeds and in vitro cultured protocorms of Oncidium bifolium sims. (Orchidaceae) by encapsulation-dehydration
Flachsland, Eduardo Alberto
Cryopreservation
Encapsulation-Dehydration
Oncidium Bifolium
Orchidaceae
Protocorms
Seeds
title_short Cryopreservation of seeds and in vitro cultured protocorms of Oncidium bifolium sims. (Orchidaceae) by encapsulation-dehydration
title_full Cryopreservation of seeds and in vitro cultured protocorms of Oncidium bifolium sims. (Orchidaceae) by encapsulation-dehydration
title_fullStr Cryopreservation of seeds and in vitro cultured protocorms of Oncidium bifolium sims. (Orchidaceae) by encapsulation-dehydration
title_full_unstemmed Cryopreservation of seeds and in vitro cultured protocorms of Oncidium bifolium sims. (Orchidaceae) by encapsulation-dehydration
title_sort Cryopreservation of seeds and in vitro cultured protocorms of Oncidium bifolium sims. (Orchidaceae) by encapsulation-dehydration
dc.creator.none.fl_str_mv Flachsland, Eduardo Alberto
Terada, Graciela
Scocchi, A.
Rey de Badaró, Hebe Yolanda
Mroginski, Luis Amado
Engelmann, F.
author Flachsland, Eduardo Alberto
author_facet Flachsland, Eduardo Alberto
Terada, Graciela
Scocchi, A.
Rey de Badaró, Hebe Yolanda
Mroginski, Luis Amado
Engelmann, F.
author_role author
author2 Terada, Graciela
Scocchi, A.
Rey de Badaró, Hebe Yolanda
Mroginski, Luis Amado
Engelmann, F.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Cryopreservation
Encapsulation-Dehydration
Oncidium Bifolium
Orchidaceae
Protocorms
Seeds
topic Cryopreservation
Encapsulation-Dehydration
Oncidium Bifolium
Orchidaceae
Protocorms
Seeds
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Encapsulation-dehydration was employed for cryopreserving seeds and in vitro-cultured protocorms of Oncidium bifolium. Freshly harvested seeds, 120 days after pollination, were encapsulated in beads containing ½ MS medium with 3% sucrose and 3% calcium alginate and subsequently pretreated in agitated (80 rpm) liquid medium supplemented with 0.15 M sucrose (24 h) followed by 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h). The beads with seeds were dehydrated with silica gel for 5 h to 19.2% moisture content and immersed in liquid nitrogen for 1 h, thawed at 30 °C for 2 min, post-treated using the same series of liquid media [0.5 M sucrose (24 h), 0.25 M sucrose (48 h), 0.15 M sucrose (24 h)], and recultured on ½ MS medium with 0.1M sucrose and 0.7% agar. As much as 4.8% of the cryopreserved seeds produced complete plants. In-vitro cultured protocorms were successfully cryopreserved following the same procedure, allowing 11.3% of them to produce plants.
Fil: Flachsland, Eduardo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Terada, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Scocchi, A.. Universidad Nacional del Nordeste; Argentina
Fil: Rey de Badaró, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Engelmann, F.. International Plant Genetic Resources Institute ; Italia
description Encapsulation-dehydration was employed for cryopreserving seeds and in vitro-cultured protocorms of Oncidium bifolium. Freshly harvested seeds, 120 days after pollination, were encapsulated in beads containing ½ MS medium with 3% sucrose and 3% calcium alginate and subsequently pretreated in agitated (80 rpm) liquid medium supplemented with 0.15 M sucrose (24 h) followed by 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h). The beads with seeds were dehydrated with silica gel for 5 h to 19.2% moisture content and immersed in liquid nitrogen for 1 h, thawed at 30 °C for 2 min, post-treated using the same series of liquid media [0.5 M sucrose (24 h), 0.25 M sucrose (48 h), 0.15 M sucrose (24 h)], and recultured on ½ MS medium with 0.1M sucrose and 0.7% agar. As much as 4.8% of the cryopreserved seeds produced complete plants. In-vitro cultured protocorms were successfully cryopreserved following the same procedure, allowing 11.3% of them to produce plants.
publishDate 2006
dc.date.none.fl_str_mv 2006-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41003
Flachsland, Eduardo Alberto; Terada, Graciela; Scocchi, A.; Rey de Badaró, Hebe Yolanda; Mroginski, Luis Amado; et al.; Cryopreservation of seeds and in vitro cultured protocorms of Oncidium bifolium sims. (Orchidaceae) by encapsulation-dehydration; Cryo Letters; Cryo-letters; 27; 4; 12-2006; 235-242
0143-2044
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41003
identifier_str_mv Flachsland, Eduardo Alberto; Terada, Graciela; Scocchi, A.; Rey de Badaró, Hebe Yolanda; Mroginski, Luis Amado; et al.; Cryopreservation of seeds and in vitro cultured protocorms of Oncidium bifolium sims. (Orchidaceae) by encapsulation-dehydration; Cryo Letters; Cryo-letters; 27; 4; 12-2006; 235-242
0143-2044
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/contentone/cryo/cryo/2006/00000027/00000004/art00005#
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cryo Letters
publisher.none.fl_str_mv Cryo Letters
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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