Chromosome stability in cryopreserved germplasm of Cyrtopodium hatschbachii (Orchidaceae)

Autores
Surenciski, Mauro Rodrigo; Dematteis, Massimiliano; Flachsland, Eduardo Alberto
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cyrtopodium hatschbachii is a recently-described orchid species distributed in norteastern Argentina. Natural populations of this species require conservation because they have a very restricted size and distribution and are in danger of destruction. Cryopreservation in liquid nitrogen is the most commonly-applied technique for ex situ conservation of plant genetic resources, but genetic stability after cryopreservation is an important consideration. Chromosome number and morphology are primary cytogenetic parameters that must remain stable after cold storage. We analyzed plants of C. hatschbachii cytogenetically in order to determinate their stability level. The plants from cryopreserved encapsulated seeds were stable at chromosome and phenotypic level, but showed more limited condensation of the chromatin during the first stages of their development. The chromosome number and karyotype analysis of C. hatschbachii is presented for the first time.
Fil: Surenciski, Mauro Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Dematteis, Massimiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Flachsland, Eduardo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Materia
Cryopreservation
Cyrtopodium
Encapsulation-Dehydration
Karyotype
Orchid Germplasm
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/45137
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/45137
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Chromosome stability in cryopreserved germplasm of Cyrtopodium hatschbachii (Orchidaceae)Surenciski, Mauro RodrigoDematteis, MassimilianoFlachsland, Eduardo AlbertoCryopreservationCyrtopodiumEncapsulation-DehydrationKaryotypeOrchid Germplasmhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cyrtopodium hatschbachii is a recently-described orchid species distributed in norteastern Argentina. Natural populations of this species require conservation because they have a very restricted size and distribution and are in danger of destruction. Cryopreservation in liquid nitrogen is the most commonly-applied technique for ex situ conservation of plant genetic resources, but genetic stability after cryopreservation is an important consideration. Chromosome number and morphology are primary cytogenetic parameters that must remain stable after cold storage. We analyzed plants of C. hatschbachii cytogenetically in order to determinate their stability level. The plants from cryopreserved encapsulated seeds were stable at chromosome and phenotypic level, but showed more limited condensation of the chromatin during the first stages of their development. The chromosome number and karyotype analysis of C. hatschbachii is presented for the first time.Fil: Surenciski, Mauro Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Dematteis, Massimiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Flachsland, Eduardo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFinnish Zoological and Botanical Publishing Board2007-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/45137Surenciski, Mauro Rodrigo; Dematteis, Massimiliano; Flachsland, Eduardo Alberto; Chromosome stability in cryopreserved germplasm of Cyrtopodium hatschbachii (Orchidaceae); Finnish Zoological and Botanical Publishing Board; Annales Botanici Fennici; 44; 4; 8-2007; 287-2920003-3847CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sekj.org/PDF/anbf44/anbf44-287.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:04:23Zoai:ri.conicet.gov.ar:11336/45137instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:04:24.211CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chromosome stability in cryopreserved germplasm of Cyrtopodium hatschbachii (Orchidaceae)
title Chromosome stability in cryopreserved germplasm of Cyrtopodium hatschbachii (Orchidaceae)
spellingShingle Chromosome stability in cryopreserved germplasm of Cyrtopodium hatschbachii (Orchidaceae)
Surenciski, Mauro Rodrigo
Cryopreservation
Cyrtopodium
Encapsulation-Dehydration
Karyotype
Orchid Germplasm
title_short Chromosome stability in cryopreserved germplasm of Cyrtopodium hatschbachii (Orchidaceae)
title_full Chromosome stability in cryopreserved germplasm of Cyrtopodium hatschbachii (Orchidaceae)
title_fullStr Chromosome stability in cryopreserved germplasm of Cyrtopodium hatschbachii (Orchidaceae)
title_full_unstemmed Chromosome stability in cryopreserved germplasm of Cyrtopodium hatschbachii (Orchidaceae)
title_sort Chromosome stability in cryopreserved germplasm of Cyrtopodium hatschbachii (Orchidaceae)
dc.creator.none.fl_str_mv Surenciski, Mauro Rodrigo
Dematteis, Massimiliano
Flachsland, Eduardo Alberto
author Surenciski, Mauro Rodrigo
author_facet Surenciski, Mauro Rodrigo
Dematteis, Massimiliano
Flachsland, Eduardo Alberto
author_role author
author2 Dematteis, Massimiliano
Flachsland, Eduardo Alberto
author2_role author
author
dc.subject.none.fl_str_mv Cryopreservation
Cyrtopodium
Encapsulation-Dehydration
Karyotype
Orchid Germplasm
topic Cryopreservation
Cyrtopodium
Encapsulation-Dehydration
Karyotype
Orchid Germplasm
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cyrtopodium hatschbachii is a recently-described orchid species distributed in norteastern Argentina. Natural populations of this species require conservation because they have a very restricted size and distribution and are in danger of destruction. Cryopreservation in liquid nitrogen is the most commonly-applied technique for ex situ conservation of plant genetic resources, but genetic stability after cryopreservation is an important consideration. Chromosome number and morphology are primary cytogenetic parameters that must remain stable after cold storage. We analyzed plants of C. hatschbachii cytogenetically in order to determinate their stability level. The plants from cryopreserved encapsulated seeds were stable at chromosome and phenotypic level, but showed more limited condensation of the chromatin during the first stages of their development. The chromosome number and karyotype analysis of C. hatschbachii is presented for the first time.
Fil: Surenciski, Mauro Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Dematteis, Massimiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Flachsland, Eduardo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
description Cyrtopodium hatschbachii is a recently-described orchid species distributed in norteastern Argentina. Natural populations of this species require conservation because they have a very restricted size and distribution and are in danger of destruction. Cryopreservation in liquid nitrogen is the most commonly-applied technique for ex situ conservation of plant genetic resources, but genetic stability after cryopreservation is an important consideration. Chromosome number and morphology are primary cytogenetic parameters that must remain stable after cold storage. We analyzed plants of C. hatschbachii cytogenetically in order to determinate their stability level. The plants from cryopreserved encapsulated seeds were stable at chromosome and phenotypic level, but showed more limited condensation of the chromatin during the first stages of their development. The chromosome number and karyotype analysis of C. hatschbachii is presented for the first time.
publishDate 2007
dc.date.none.fl_str_mv 2007-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/45137
Surenciski, Mauro Rodrigo; Dematteis, Massimiliano; Flachsland, Eduardo Alberto; Chromosome stability in cryopreserved germplasm of Cyrtopodium hatschbachii (Orchidaceae); Finnish Zoological and Botanical Publishing Board; Annales Botanici Fennici; 44; 4; 8-2007; 287-292
0003-3847
CONICET Digital
CONICET
url http://hdl.handle.net/11336/45137
identifier_str_mv Surenciski, Mauro Rodrigo; Dematteis, Massimiliano; Flachsland, Eduardo Alberto; Chromosome stability in cryopreserved germplasm of Cyrtopodium hatschbachii (Orchidaceae); Finnish Zoological and Botanical Publishing Board; Annales Botanici Fennici; 44; 4; 8-2007; 287-292
0003-3847
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sekj.org/PDF/anbf44/anbf44-287.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Finnish Zoological and Botanical Publishing Board
publisher.none.fl_str_mv Finnish Zoological and Botanical Publishing Board
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781300419067904
score 12.982451

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