Cryopreservation of Arachis Pintoi (leguminosae) Somatic Embryos

Autores
Rey, Hebe Yolanda; Faloci, Mirta Mabel; Medina, Ricardo Daniel; Dolce, Natalia Raquel; Engelmann, Florent; Mroginski, Luis Amado
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this study, we successfully cryopreserved cotyledonary somatic embryos of diploid and triploid Arachis pintoi cytotypes using the encapsulation-dehydration technique. The highest survival rates were obtained when somatic embryos were encapsulated in calcium alginate beads and precultured in agitated (80 rpm) liquid establishment medium (EM) with daily increasing sucrose concentration (0.50, 0.75, and 1.0 M). The encapsulated somatic embryos were then dehydrated with silica gel for 5 h to 20% moisture content (fresh weight basis) and cooled either rapidly (direct immersion in liquid nitrogen, LN) or slowly (1ºC min-1 from 25ºC to -30ºC followed by immersion in LN). Beads were kept in LN for a minimum of 1 h and then were rapidly rewarmed in a 30ºC water-bath for 2 min. Finally, encapsulated somatic embryos were post-cultured in agitated (80 rpm) liquid EM with daily decreasing sucrose concentration (0.75 and 0.5 M) and transferred to solidified EM. Using this protocol, we obtained 26% and 30% plant regeneration from cryopreserved somatic embryos of diploid and triploid cytotypes. No morphological abnormalities were observed in any of the plants regenerated from cryopreserved embryos and their genetic stability was confirmed with 10 isozyme systems and nine RAPD profiles.
Fil: Rey, Hebe Yolanda. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Rey, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica; Argentina.
Fil: Faloci, Mirta Mabel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Faloci, Mirta Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Medina, Ricardo D. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Medina, Ricardo D. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Medina, Ricardo D. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Dolce, Natalia Raquel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Dolce, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Engelmann, Florent. Centre National de la Recherche Scientifique. Institut de Recherche Pour Le Developpement; Francia.
Fil: Mroginski, Luis Amado. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fuente
CryoLetters, 2013, vol. 34, no. 6, p. 571-582.
Materia
Somatic embryo
Cryopreservation
Encapsulation-dehydration
Genetic stability
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/9159
Acceder
id RIUNNE_29833385f21200ea61f8d1ba690d53ac
oai_identifier_str oai:repositorio.unne.edu.ar:123456789/9159
network_acronym_str RIUNNE
repository_id_str 4871
network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling Cryopreservation of Arachis Pintoi (leguminosae) Somatic EmbryosRey, Hebe YolandaFaloci, Mirta MabelMedina, Ricardo DanielDolce, Natalia RaquelEngelmann, FlorentMroginski, Luis AmadoSomatic embryoCryopreservationEncapsulation-dehydrationGenetic stabilityIn this study, we successfully cryopreserved cotyledonary somatic embryos of diploid and triploid Arachis pintoi cytotypes using the encapsulation-dehydration technique. The highest survival rates were obtained when somatic embryos were encapsulated in calcium alginate beads and precultured in agitated (80 rpm) liquid establishment medium (EM) with daily increasing sucrose concentration (0.50, 0.75, and 1.0 M). The encapsulated somatic embryos were then dehydrated with silica gel for 5 h to 20% moisture content (fresh weight basis) and cooled either rapidly (direct immersion in liquid nitrogen, LN) or slowly (1ºC min-1 from 25ºC to -30ºC followed by immersion in LN). Beads were kept in LN for a minimum of 1 h and then were rapidly rewarmed in a 30ºC water-bath for 2 min. Finally, encapsulated somatic embryos were post-cultured in agitated (80 rpm) liquid EM with daily decreasing sucrose concentration (0.75 and 0.5 M) and transferred to solidified EM. Using this protocol, we obtained 26% and 30% plant regeneration from cryopreserved somatic embryos of diploid and triploid cytotypes. No morphological abnormalities were observed in any of the plants regenerated from cryopreserved embryos and their genetic stability was confirmed with 10 isozyme systems and nine RAPD profiles.Fil: Rey, Hebe Yolanda. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Rey, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica; Argentina.Fil: Faloci, Mirta Mabel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Faloci, Mirta Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Medina, Ricardo D. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Medina, Ricardo D. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Medina, Ricardo D. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Dolce, Natalia Raquel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Dolce, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Engelmann, Florent. Centre National de la Recherche Scientifique. Institut de Recherche Pour Le Developpement; Francia.Fil: Mroginski, Luis Amado. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.CryoLetters2013-01-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfp. 571-582application/pdfRey, Hebe Y., et al, 2013. Cryopreservation of arachis pintoi (leguminosae) somatic embryos. Cryoletters. Lewes: Cryoletters , vol. 34, no. 6, p. 571-582. ISSN 1742-0644.0143-2044http://repositorio.unne.edu.ar/handle/123456789/9159CryoLetters, 2013, vol. 34, no. 6, p. 571-582.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordesteenghttp://www.cryoletters.org/Abstracts/vol_34_6_2013.htm#571info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2025-09-29T14:30:38Zoai:repositorio.unne.edu.ar:123456789/9159instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712025-09-29 14:30:38.672Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv Cryopreservation of Arachis Pintoi (leguminosae) Somatic Embryos
title Cryopreservation of Arachis Pintoi (leguminosae) Somatic Embryos
spellingShingle Cryopreservation of Arachis Pintoi (leguminosae) Somatic Embryos
Rey, Hebe Yolanda
Somatic embryo
Cryopreservation
Encapsulation-dehydration
Genetic stability
title_short Cryopreservation of Arachis Pintoi (leguminosae) Somatic Embryos
title_full Cryopreservation of Arachis Pintoi (leguminosae) Somatic Embryos
title_fullStr Cryopreservation of Arachis Pintoi (leguminosae) Somatic Embryos
title_full_unstemmed Cryopreservation of Arachis Pintoi (leguminosae) Somatic Embryos
title_sort Cryopreservation of Arachis Pintoi (leguminosae) Somatic Embryos
dc.creator.none.fl_str_mv Rey, Hebe Yolanda
Faloci, Mirta Mabel
Medina, Ricardo Daniel
Dolce, Natalia Raquel
Engelmann, Florent
Mroginski, Luis Amado
author Rey, Hebe Yolanda
author_facet Rey, Hebe Yolanda
Faloci, Mirta Mabel
Medina, Ricardo Daniel
Dolce, Natalia Raquel
Engelmann, Florent
Mroginski, Luis Amado
author_role author
author2 Faloci, Mirta Mabel
Medina, Ricardo Daniel
Dolce, Natalia Raquel
Engelmann, Florent
Mroginski, Luis Amado
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Somatic embryo
Cryopreservation
Encapsulation-dehydration
Genetic stability
topic Somatic embryo
Cryopreservation
Encapsulation-dehydration
Genetic stability
dc.description.none.fl_txt_mv In this study, we successfully cryopreserved cotyledonary somatic embryos of diploid and triploid Arachis pintoi cytotypes using the encapsulation-dehydration technique. The highest survival rates were obtained when somatic embryos were encapsulated in calcium alginate beads and precultured in agitated (80 rpm) liquid establishment medium (EM) with daily increasing sucrose concentration (0.50, 0.75, and 1.0 M). The encapsulated somatic embryos were then dehydrated with silica gel for 5 h to 20% moisture content (fresh weight basis) and cooled either rapidly (direct immersion in liquid nitrogen, LN) or slowly (1ºC min-1 from 25ºC to -30ºC followed by immersion in LN). Beads were kept in LN for a minimum of 1 h and then were rapidly rewarmed in a 30ºC water-bath for 2 min. Finally, encapsulated somatic embryos were post-cultured in agitated (80 rpm) liquid EM with daily decreasing sucrose concentration (0.75 and 0.5 M) and transferred to solidified EM. Using this protocol, we obtained 26% and 30% plant regeneration from cryopreserved somatic embryos of diploid and triploid cytotypes. No morphological abnormalities were observed in any of the plants regenerated from cryopreserved embryos and their genetic stability was confirmed with 10 isozyme systems and nine RAPD profiles.
Fil: Rey, Hebe Yolanda. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Rey, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica; Argentina.
Fil: Faloci, Mirta Mabel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Faloci, Mirta Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Medina, Ricardo D. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Medina, Ricardo D. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Medina, Ricardo D. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Dolce, Natalia Raquel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Dolce, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Engelmann, Florent. Centre National de la Recherche Scientifique. Institut de Recherche Pour Le Developpement; Francia.
Fil: Mroginski, Luis Amado. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
description In this study, we successfully cryopreserved cotyledonary somatic embryos of diploid and triploid Arachis pintoi cytotypes using the encapsulation-dehydration technique. The highest survival rates were obtained when somatic embryos were encapsulated in calcium alginate beads and precultured in agitated (80 rpm) liquid establishment medium (EM) with daily increasing sucrose concentration (0.50, 0.75, and 1.0 M). The encapsulated somatic embryos were then dehydrated with silica gel for 5 h to 20% moisture content (fresh weight basis) and cooled either rapidly (direct immersion in liquid nitrogen, LN) or slowly (1ºC min-1 from 25ºC to -30ºC followed by immersion in LN). Beads were kept in LN for a minimum of 1 h and then were rapidly rewarmed in a 30ºC water-bath for 2 min. Finally, encapsulated somatic embryos were post-cultured in agitated (80 rpm) liquid EM with daily decreasing sucrose concentration (0.75 and 0.5 M) and transferred to solidified EM. Using this protocol, we obtained 26% and 30% plant regeneration from cryopreserved somatic embryos of diploid and triploid cytotypes. No morphological abnormalities were observed in any of the plants regenerated from cryopreserved embryos and their genetic stability was confirmed with 10 isozyme systems and nine RAPD profiles.
publishDate 2013
dc.date.none.fl_str_mv 2013-01-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Rey, Hebe Y., et al, 2013. Cryopreservation of arachis pintoi (leguminosae) somatic embryos. Cryoletters. Lewes: Cryoletters , vol. 34, no. 6, p. 571-582. ISSN 1742-0644.
0143-2044
http://repositorio.unne.edu.ar/handle/123456789/9159
identifier_str_mv Rey, Hebe Y., et al, 2013. Cryopreservation of arachis pintoi (leguminosae) somatic embryos. Cryoletters. Lewes: Cryoletters , vol. 34, no. 6, p. 571-582. ISSN 1742-0644.
0143-2044
url http://repositorio.unne.edu.ar/handle/123456789/9159
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://www.cryoletters.org/Abstracts/vol_34_6_2013.htm#571
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
p. 571-582
application/pdf
dc.publisher.none.fl_str_mv CryoLetters
publisher.none.fl_str_mv CryoLetters
dc.source.none.fl_str_mv CryoLetters, 2013, vol. 34, no. 6, p. 571-582.
reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
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score 12.559606

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