Cryopreservation of in vitro grown shoot tips and apical meristems of the forage legume Arachis pintoi

Autores
Rey de Badaró, Hebe Yolanda; Faloci, Mirta Mabel; Medina, Ricardo Daniel; Dolce, Natalia Raquel; Mroginski, Luis Amado; Engelmann, Florent
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A cryopreservation protocol using the encapsulation-dehydration procedure was established for shoot tips (2-3 mm in length) and meristems (0.3-0.5 mm) sampled from in vitro plantlets of diploid and triploid cytotypes of Arachis pintoi. The optimal protocol was the following: after dissection, explants were precultured for 24 h on establishment medium (EM), encapsulated in calcium alginate beads and pretreated in liquid EM medium with daily increasing sucrose concentration (0.5, 0.75, 1.0 M) and desiccated to 22-23% moisture content (fresh weight basis). Explants were frozen using slow cooling (1ºC min-1 from 25ºC to -30ºC followed by direct immersion in liquid nitrogen), thawed rapidly and post-cultured in liquid EM medium enriched with daily decreasing sucrose concentrations (0.75, 0.50, 0.1 M). Explants were then transferred to solid EM medium in order to achieve shoot regeneration, then on Murashige and Skoog medium supplemented with 0.05 μM naphthalene acetic acid to induce rooting of shoots. With this procedure, 53% and 56% of cryopreserved shoot tips of the diploid and triploid cytotypes, respectively, survived and formed plants. However, Orly 16% of cryopreserved meristems of both cytotypes regenerated plants. Using ten isozyme systems and seven RAPD profiles, no modification induced by cryopreservation could be detected in plantlets regenerated from cryopreserved material.
Fil: Rey de Badaró, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Faloci, Mirta Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina
Fil: Medina, Ricardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Dolce, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Engelmann, Florent. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia
Materia
Apical Meristems
Cryopreservation
Encapsulation-Dehydration
Genetic Stability
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/46756
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Cryopreservation of in vitro grown shoot tips and apical meristems of the forage legume Arachis pintoiRey de Badaró, Hebe YolandaFaloci, Mirta MabelMedina, Ricardo DanielDolce, Natalia RaquelMroginski, Luis AmadoEngelmann, FlorentApical MeristemsCryopreservationEncapsulation-DehydrationGenetic Stabilityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A cryopreservation protocol using the encapsulation-dehydration procedure was established for shoot tips (2-3 mm in length) and meristems (0.3-0.5 mm) sampled from in vitro plantlets of diploid and triploid cytotypes of Arachis pintoi. The optimal protocol was the following: after dissection, explants were precultured for 24 h on establishment medium (EM), encapsulated in calcium alginate beads and pretreated in liquid EM medium with daily increasing sucrose concentration (0.5, 0.75, 1.0 M) and desiccated to 22-23% moisture content (fresh weight basis). Explants were frozen using slow cooling (1ºC min-1 from 25ºC to -30ºC followed by direct immersion in liquid nitrogen), thawed rapidly and post-cultured in liquid EM medium enriched with daily decreasing sucrose concentrations (0.75, 0.50, 0.1 M). Explants were then transferred to solid EM medium in order to achieve shoot regeneration, then on Murashige and Skoog medium supplemented with 0.05 μM naphthalene acetic acid to induce rooting of shoots. With this procedure, 53% and 56% of cryopreserved shoot tips of the diploid and triploid cytotypes, respectively, survived and formed plants. However, Orly 16% of cryopreserved meristems of both cytotypes regenerated plants. Using ten isozyme systems and seven RAPD profiles, no modification induced by cryopreservation could be detected in plantlets regenerated from cryopreserved material.Fil: Rey de Badaró, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Faloci, Mirta Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; ArgentinaFil: Medina, Ricardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Dolce, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Engelmann, Florent. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; FranciaCryo Letters2009-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/46756Rey de Badaró, Hebe Yolanda; Faloci, Mirta Mabel; Medina, Ricardo Daniel; Dolce, Natalia Raquel; Mroginski, Luis Amado; et al.; Cryopreservation of in vitro grown shoot tips and apical meristems of the forage legume Arachis pintoi; Cryo Letters; Cryo-letters; 30; 5; 12-2009; 347-3580143-20441742-0644CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.cryoletters.org/Abstracts/vol_30_5_2009.htm#347info:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/contentone/cryo/cryo/2009/00000030/00000005/art00004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:44:56Zoai:ri.conicet.gov.ar:11336/46756instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:44:56.682CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cryopreservation of in vitro grown shoot tips and apical meristems of the forage legume Arachis pintoi
title Cryopreservation of in vitro grown shoot tips and apical meristems of the forage legume Arachis pintoi
spellingShingle Cryopreservation of in vitro grown shoot tips and apical meristems of the forage legume Arachis pintoi
Rey de Badaró, Hebe Yolanda
Apical Meristems
Cryopreservation
Encapsulation-Dehydration
Genetic Stability
title_short Cryopreservation of in vitro grown shoot tips and apical meristems of the forage legume Arachis pintoi
title_full Cryopreservation of in vitro grown shoot tips and apical meristems of the forage legume Arachis pintoi
title_fullStr Cryopreservation of in vitro grown shoot tips and apical meristems of the forage legume Arachis pintoi
title_full_unstemmed Cryopreservation of in vitro grown shoot tips and apical meristems of the forage legume Arachis pintoi
title_sort Cryopreservation of in vitro grown shoot tips and apical meristems of the forage legume Arachis pintoi
dc.creator.none.fl_str_mv Rey de Badaró, Hebe Yolanda
Faloci, Mirta Mabel
Medina, Ricardo Daniel
Dolce, Natalia Raquel
Mroginski, Luis Amado
Engelmann, Florent
author Rey de Badaró, Hebe Yolanda
author_facet Rey de Badaró, Hebe Yolanda
Faloci, Mirta Mabel
Medina, Ricardo Daniel
Dolce, Natalia Raquel
Mroginski, Luis Amado
Engelmann, Florent
author_role author
author2 Faloci, Mirta Mabel
Medina, Ricardo Daniel
Dolce, Natalia Raquel
Mroginski, Luis Amado
Engelmann, Florent
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Apical Meristems
Cryopreservation
Encapsulation-Dehydration
Genetic Stability
topic Apical Meristems
Cryopreservation
Encapsulation-Dehydration
Genetic Stability
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A cryopreservation protocol using the encapsulation-dehydration procedure was established for shoot tips (2-3 mm in length) and meristems (0.3-0.5 mm) sampled from in vitro plantlets of diploid and triploid cytotypes of Arachis pintoi. The optimal protocol was the following: after dissection, explants were precultured for 24 h on establishment medium (EM), encapsulated in calcium alginate beads and pretreated in liquid EM medium with daily increasing sucrose concentration (0.5, 0.75, 1.0 M) and desiccated to 22-23% moisture content (fresh weight basis). Explants were frozen using slow cooling (1ºC min-1 from 25ºC to -30ºC followed by direct immersion in liquid nitrogen), thawed rapidly and post-cultured in liquid EM medium enriched with daily decreasing sucrose concentrations (0.75, 0.50, 0.1 M). Explants were then transferred to solid EM medium in order to achieve shoot regeneration, then on Murashige and Skoog medium supplemented with 0.05 μM naphthalene acetic acid to induce rooting of shoots. With this procedure, 53% and 56% of cryopreserved shoot tips of the diploid and triploid cytotypes, respectively, survived and formed plants. However, Orly 16% of cryopreserved meristems of both cytotypes regenerated plants. Using ten isozyme systems and seven RAPD profiles, no modification induced by cryopreservation could be detected in plantlets regenerated from cryopreserved material.
Fil: Rey de Badaró, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Faloci, Mirta Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina
Fil: Medina, Ricardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Dolce, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Engelmann, Florent. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia
description A cryopreservation protocol using the encapsulation-dehydration procedure was established for shoot tips (2-3 mm in length) and meristems (0.3-0.5 mm) sampled from in vitro plantlets of diploid and triploid cytotypes of Arachis pintoi. The optimal protocol was the following: after dissection, explants were precultured for 24 h on establishment medium (EM), encapsulated in calcium alginate beads and pretreated in liquid EM medium with daily increasing sucrose concentration (0.5, 0.75, 1.0 M) and desiccated to 22-23% moisture content (fresh weight basis). Explants were frozen using slow cooling (1ºC min-1 from 25ºC to -30ºC followed by direct immersion in liquid nitrogen), thawed rapidly and post-cultured in liquid EM medium enriched with daily decreasing sucrose concentrations (0.75, 0.50, 0.1 M). Explants were then transferred to solid EM medium in order to achieve shoot regeneration, then on Murashige and Skoog medium supplemented with 0.05 μM naphthalene acetic acid to induce rooting of shoots. With this procedure, 53% and 56% of cryopreserved shoot tips of the diploid and triploid cytotypes, respectively, survived and formed plants. However, Orly 16% of cryopreserved meristems of both cytotypes regenerated plants. Using ten isozyme systems and seven RAPD profiles, no modification induced by cryopreservation could be detected in plantlets regenerated from cryopreserved material.
publishDate 2009
dc.date.none.fl_str_mv 2009-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/46756
Rey de Badaró, Hebe Yolanda; Faloci, Mirta Mabel; Medina, Ricardo Daniel; Dolce, Natalia Raquel; Mroginski, Luis Amado; et al.; Cryopreservation of in vitro grown shoot tips and apical meristems of the forage legume Arachis pintoi; Cryo Letters; Cryo-letters; 30; 5; 12-2009; 347-358
0143-2044
1742-0644
CONICET Digital
CONICET
url http://hdl.handle.net/11336/46756
identifier_str_mv Rey de Badaró, Hebe Yolanda; Faloci, Mirta Mabel; Medina, Ricardo Daniel; Dolce, Natalia Raquel; Mroginski, Luis Amado; et al.; Cryopreservation of in vitro grown shoot tips and apical meristems of the forage legume Arachis pintoi; Cryo Letters; Cryo-letters; 30; 5; 12-2009; 347-358
0143-2044
1742-0644
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.cryoletters.org/Abstracts/vol_30_5_2009.htm#347
info:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/contentone/cryo/cryo/2009/00000030/00000005/art00004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cryo Letters
publisher.none.fl_str_mv Cryo Letters
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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