Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera based on its whole-genome analysis
- Autores
- Lazarte, Jorge Nicolás; Poliero, Aimará Ayelen; Berón, Corina Marta
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- The insecticidal proteins of Bacillus thuringiensis are used as formulations of spore-crystal complexes and their genes have been incorporated into several crops, which has provided a model for genetic engineering in agriculture. Despite the variability of the Cry proteins described so far, it is still necessary to look for toxins with a broad spectrum of action, since a significant number of pests are not controlled with the available Cry proteins. It is also important to provide alternatives to address the problem of insect resistance, which has already appeared with the use of formulations and in transgenic plants that express cry genes that code for insecticidal proteins. We report the characterization of a novel B. thuringiensis isolate native to Argentina (FCC7) toxic against lepidoptera and coleoptera insects. The strain shows a rounded crystal harboring mainly a protein of about 130 kDa. Through the whole-genome sequencing by Illumina Miseq 1500 platform we detected two crystal protein genes with cry8-like genes homology, three vegetative insecticidal proteins (Vips) genes and multiple virulence factors such as phospholipases, proteases, enhancins, chitinases, among others. The two cry8-like genes, homologues of Cry8Ac1 and Cry8Qa1 sequences with 73,4 % and 88,9 % of identity respectively, were cloned and expressed into the 4Q7/pSTAB system and the larvicidal activity were assayed against Spodoptera frugiperda and Tenebrio molitor. Two of the Vips genes were identified as Vip1-like with 74,9% and 70,3% identity respectively while the third Vip gene was 82% identical to Vip2 sequences.
Fil: Lazarte, Jorge Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Poliero, Aimará Ayelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Berón, Corina Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
XIV Encuentro Biólog@s En Red: 14 años por una ciencia hecha entre todes y para todes
Mar del Plata
Argentina
Asociación de Jóvenes Investigadores en Formación - Materia
-
BACILLUS THURINGIENSIS
WHOLE GENOME ANALYSIS
BIOLOGICAL CONTROL - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/138695
Ver los metadatos del registro completo
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Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera based on its whole-genome analysisLazarte, Jorge NicolásPoliero, Aimará AyelenBerón, Corina MartaBACILLUS THURINGIENSISWHOLE GENOME ANALYSISBIOLOGICAL CONTROLhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The insecticidal proteins of Bacillus thuringiensis are used as formulations of spore-crystal complexes and their genes have been incorporated into several crops, which has provided a model for genetic engineering in agriculture. Despite the variability of the Cry proteins described so far, it is still necessary to look for toxins with a broad spectrum of action, since a significant number of pests are not controlled with the available Cry proteins. It is also important to provide alternatives to address the problem of insect resistance, which has already appeared with the use of formulations and in transgenic plants that express cry genes that code for insecticidal proteins. We report the characterization of a novel B. thuringiensis isolate native to Argentina (FCC7) toxic against lepidoptera and coleoptera insects. The strain shows a rounded crystal harboring mainly a protein of about 130 kDa. Through the whole-genome sequencing by Illumina Miseq 1500 platform we detected two crystal protein genes with cry8-like genes homology, three vegetative insecticidal proteins (Vips) genes and multiple virulence factors such as phospholipases, proteases, enhancins, chitinases, among others. The two cry8-like genes, homologues of Cry8Ac1 and Cry8Qa1 sequences with 73,4 % and 88,9 % of identity respectively, were cloned and expressed into the 4Q7/pSTAB system and the larvicidal activity were assayed against Spodoptera frugiperda and Tenebrio molitor. Two of the Vips genes were identified as Vip1-like with 74,9% and 70,3% identity respectively while the third Vip gene was 82% identical to Vip2 sequences.Fil: Lazarte, Jorge Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaFil: Poliero, Aimará Ayelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaFil: Berón, Corina Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaXIV Encuentro Biólog@s En Red: 14 años por una ciencia hecha entre todes y para todesMar del PlataArgentinaAsociación de Jóvenes Investigadores en FormaciónAsociación de Jóvenes Investigadores en Formación2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectEncuentroJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/138695Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera based on its whole-genome analysis; XIV Encuentro Biólog@s En Red: 14 años por una ciencia hecha entre todes y para todes; Mar del Plata; Argentina; 2019; 42-421853-9998CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://xiv-ber-2019.blogspot.com/2019/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:19:48Zoai:ri.conicet.gov.ar:11336/138695instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:19:49.173CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera based on its whole-genome analysis |
title |
Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera based on its whole-genome analysis |
spellingShingle |
Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera based on its whole-genome analysis Lazarte, Jorge Nicolás BACILLUS THURINGIENSIS WHOLE GENOME ANALYSIS BIOLOGICAL CONTROL |
title_short |
Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera based on its whole-genome analysis |
title_full |
Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera based on its whole-genome analysis |
title_fullStr |
Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera based on its whole-genome analysis |
title_full_unstemmed |
Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera based on its whole-genome analysis |
title_sort |
Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera based on its whole-genome analysis |
dc.creator.none.fl_str_mv |
Lazarte, Jorge Nicolás Poliero, Aimará Ayelen Berón, Corina Marta |
author |
Lazarte, Jorge Nicolás |
author_facet |
Lazarte, Jorge Nicolás Poliero, Aimará Ayelen Berón, Corina Marta |
author_role |
author |
author2 |
Poliero, Aimará Ayelen Berón, Corina Marta |
author2_role |
author author |
dc.subject.none.fl_str_mv |
BACILLUS THURINGIENSIS WHOLE GENOME ANALYSIS BIOLOGICAL CONTROL |
topic |
BACILLUS THURINGIENSIS WHOLE GENOME ANALYSIS BIOLOGICAL CONTROL |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The insecticidal proteins of Bacillus thuringiensis are used as formulations of spore-crystal complexes and their genes have been incorporated into several crops, which has provided a model for genetic engineering in agriculture. Despite the variability of the Cry proteins described so far, it is still necessary to look for toxins with a broad spectrum of action, since a significant number of pests are not controlled with the available Cry proteins. It is also important to provide alternatives to address the problem of insect resistance, which has already appeared with the use of formulations and in transgenic plants that express cry genes that code for insecticidal proteins. We report the characterization of a novel B. thuringiensis isolate native to Argentina (FCC7) toxic against lepidoptera and coleoptera insects. The strain shows a rounded crystal harboring mainly a protein of about 130 kDa. Through the whole-genome sequencing by Illumina Miseq 1500 platform we detected two crystal protein genes with cry8-like genes homology, three vegetative insecticidal proteins (Vips) genes and multiple virulence factors such as phospholipases, proteases, enhancins, chitinases, among others. The two cry8-like genes, homologues of Cry8Ac1 and Cry8Qa1 sequences with 73,4 % and 88,9 % of identity respectively, were cloned and expressed into the 4Q7/pSTAB system and the larvicidal activity were assayed against Spodoptera frugiperda and Tenebrio molitor. Two of the Vips genes were identified as Vip1-like with 74,9% and 70,3% identity respectively while the third Vip gene was 82% identical to Vip2 sequences. Fil: Lazarte, Jorge Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina Fil: Poliero, Aimará Ayelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina Fil: Berón, Corina Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina XIV Encuentro Biólog@s En Red: 14 años por una ciencia hecha entre todes y para todes Mar del Plata Argentina Asociación de Jóvenes Investigadores en Formación |
description |
The insecticidal proteins of Bacillus thuringiensis are used as formulations of spore-crystal complexes and their genes have been incorporated into several crops, which has provided a model for genetic engineering in agriculture. Despite the variability of the Cry proteins described so far, it is still necessary to look for toxins with a broad spectrum of action, since a significant number of pests are not controlled with the available Cry proteins. It is also important to provide alternatives to address the problem of insect resistance, which has already appeared with the use of formulations and in transgenic plants that express cry genes that code for insecticidal proteins. We report the characterization of a novel B. thuringiensis isolate native to Argentina (FCC7) toxic against lepidoptera and coleoptera insects. The strain shows a rounded crystal harboring mainly a protein of about 130 kDa. Through the whole-genome sequencing by Illumina Miseq 1500 platform we detected two crystal protein genes with cry8-like genes homology, three vegetative insecticidal proteins (Vips) genes and multiple virulence factors such as phospholipases, proteases, enhancins, chitinases, among others. The two cry8-like genes, homologues of Cry8Ac1 and Cry8Qa1 sequences with 73,4 % and 88,9 % of identity respectively, were cloned and expressed into the 4Q7/pSTAB system and the larvicidal activity were assayed against Spodoptera frugiperda and Tenebrio molitor. Two of the Vips genes were identified as Vip1-like with 74,9% and 70,3% identity respectively while the third Vip gene was 82% identical to Vip2 sequences. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Encuentro Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/138695 Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera based on its whole-genome analysis; XIV Encuentro Biólog@s En Red: 14 años por una ciencia hecha entre todes y para todes; Mar del Plata; Argentina; 2019; 42-42 1853-9998 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/138695 |
identifier_str_mv |
Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera based on its whole-genome analysis; XIV Encuentro Biólog@s En Red: 14 años por una ciencia hecha entre todes y para todes; Mar del Plata; Argentina; 2019; 42-42 1853-9998 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://xiv-ber-2019.blogspot.com/2019/ |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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application/pdf application/pdf application/pdf |
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Nacional |
dc.publisher.none.fl_str_mv |
Asociación de Jóvenes Investigadores en Formación |
publisher.none.fl_str_mv |
Asociación de Jóvenes Investigadores en Formación |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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