Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera based on its whole-genome analysis

Autores
Lazarte, Jorge Nicolás; Poliero, Aimará Ayelen; Berón, Corina Marta
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The insecticidal proteins of Bacillus thuringiensis are used as formulations of spore-crystal complexes and their genes have been incorporated into several crops, which has provided a model for genetic engineering in agriculture. Despite the variability of the Cry proteins described so far, it is still necessary to look for toxins with a broad spectrum of action, since a significant number of pests are not controlled with the available Cry proteins. It is also important to provide alternatives to address the problem of insect resistance, which has already appeared with the use of formulations and in transgenic plants that express cry genes that code for insecticidal proteins. We report the characterization of a novel B. thuringiensis isolate native to Argentina (FCC7) toxic against lepidoptera and coleoptera insects. The strain shows a rounded crystal harboring mainly a protein of about 130 kDa. Through the whole-genome sequencing by Illumina Miseq 1500 platform we detected two crystal protein genes with cry8-like genes homology, three vegetative insecticidal proteins (Vips) genes and multiple virulence factors such as phospholipases, proteases, enhancins, chitinases, among others. The two cry8-like genes, homologues of Cry8Ac1 and Cry8Qa1 sequences with 73,4 % and 88,9 % of identity respectively, were cloned and expressed into the 4Q7/pSTAB system and the larvicidal activity were assayed against Spodoptera frugiperda and Tenebrio molitor. Two of the Vips genes were identified as Vip1-like with 74,9% and 70,3% identity respectively while the third Vip gene was 82% identical to Vip2 sequences.
Fil: Lazarte, Jorge Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Poliero, Aimará Ayelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Berón, Corina Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
XIV Encuentro Biólog@s En Red: 14 años por una ciencia hecha entre todes y para todes
Mar del Plata
Argentina
Asociación de Jóvenes Investigadores en Formación
Materia
BACILLUS THURINGIENSIS
WHOLE GENOME ANALYSIS
BIOLOGICAL CONTROL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/138695
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/138695
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera based on its whole-genome analysisLazarte, Jorge NicolásPoliero, Aimará AyelenBerón, Corina MartaBACILLUS THURINGIENSISWHOLE GENOME ANALYSISBIOLOGICAL CONTROLhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The insecticidal proteins of Bacillus thuringiensis are used as formulations of spore-crystal complexes and their genes have been incorporated into several crops, which has provided a model for genetic engineering in agriculture. Despite the variability of the Cry proteins described so far, it is still necessary to look for toxins with a broad spectrum of action, since a significant number of pests are not controlled with the available Cry proteins. It is also important to provide alternatives to address the problem of insect resistance, which has already appeared with the use of formulations and in transgenic plants that express cry genes that code for insecticidal proteins. We report the characterization of a novel B. thuringiensis isolate native to Argentina (FCC7) toxic against lepidoptera and coleoptera insects. The strain shows a rounded crystal harboring mainly a protein of about 130 kDa. Through the whole-genome sequencing by Illumina Miseq 1500 platform we detected two crystal protein genes with cry8-like genes homology, three vegetative insecticidal proteins (Vips) genes and multiple virulence factors such as phospholipases, proteases, enhancins, chitinases, among others. The two cry8-like genes, homologues of Cry8Ac1 and Cry8Qa1 sequences with 73,4 % and 88,9 % of identity respectively, were cloned and expressed into the 4Q7/pSTAB system and the larvicidal activity were assayed against Spodoptera frugiperda and Tenebrio molitor. Two of the Vips genes were identified as Vip1-like with 74,9% and 70,3% identity respectively while the third Vip gene was 82% identical to Vip2 sequences.Fil: Lazarte, Jorge Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaFil: Poliero, Aimará Ayelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaFil: Berón, Corina Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaXIV Encuentro Biólog@s En Red: 14 años por una ciencia hecha entre todes y para todesMar del PlataArgentinaAsociación de Jóvenes Investigadores en FormaciónAsociación de Jóvenes Investigadores en Formación2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectEncuentroJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/138695Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera based on its whole-genome analysis; XIV Encuentro Biólog@s En Red: 14 años por una ciencia hecha entre todes y para todes; Mar del Plata; Argentina; 2019; 42-421853-9998CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://xiv-ber-2019.blogspot.com/2019/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:52:29Zoai:ri.conicet.gov.ar:11336/138695instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:52:29.668CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera based on its whole-genome analysis
title Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera based on its whole-genome analysis
spellingShingle Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera based on its whole-genome analysis
Lazarte, Jorge Nicolás
BACILLUS THURINGIENSIS
WHOLE GENOME ANALYSIS
BIOLOGICAL CONTROL
title_short Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera based on its whole-genome analysis
title_full Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera based on its whole-genome analysis
title_fullStr Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera based on its whole-genome analysis
title_full_unstemmed Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera based on its whole-genome analysis
title_sort Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera based on its whole-genome analysis
dc.creator.none.fl_str_mv Lazarte, Jorge Nicolás
Poliero, Aimará Ayelen
Berón, Corina Marta
author Lazarte, Jorge Nicolás
author_facet Lazarte, Jorge Nicolás
Poliero, Aimará Ayelen
Berón, Corina Marta
author_role author
author2 Poliero, Aimará Ayelen
Berón, Corina Marta
author2_role author
author
dc.subject.none.fl_str_mv BACILLUS THURINGIENSIS
WHOLE GENOME ANALYSIS
BIOLOGICAL CONTROL
topic BACILLUS THURINGIENSIS
WHOLE GENOME ANALYSIS
BIOLOGICAL CONTROL
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The insecticidal proteins of Bacillus thuringiensis are used as formulations of spore-crystal complexes and their genes have been incorporated into several crops, which has provided a model for genetic engineering in agriculture. Despite the variability of the Cry proteins described so far, it is still necessary to look for toxins with a broad spectrum of action, since a significant number of pests are not controlled with the available Cry proteins. It is also important to provide alternatives to address the problem of insect resistance, which has already appeared with the use of formulations and in transgenic plants that express cry genes that code for insecticidal proteins. We report the characterization of a novel B. thuringiensis isolate native to Argentina (FCC7) toxic against lepidoptera and coleoptera insects. The strain shows a rounded crystal harboring mainly a protein of about 130 kDa. Through the whole-genome sequencing by Illumina Miseq 1500 platform we detected two crystal protein genes with cry8-like genes homology, three vegetative insecticidal proteins (Vips) genes and multiple virulence factors such as phospholipases, proteases, enhancins, chitinases, among others. The two cry8-like genes, homologues of Cry8Ac1 and Cry8Qa1 sequences with 73,4 % and 88,9 % of identity respectively, were cloned and expressed into the 4Q7/pSTAB system and the larvicidal activity were assayed against Spodoptera frugiperda and Tenebrio molitor. Two of the Vips genes were identified as Vip1-like with 74,9% and 70,3% identity respectively while the third Vip gene was 82% identical to Vip2 sequences.
Fil: Lazarte, Jorge Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Poliero, Aimará Ayelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Berón, Corina Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
XIV Encuentro Biólog@s En Red: 14 años por una ciencia hecha entre todes y para todes
Mar del Plata
Argentina
Asociación de Jóvenes Investigadores en Formación
description The insecticidal proteins of Bacillus thuringiensis are used as formulations of spore-crystal complexes and their genes have been incorporated into several crops, which has provided a model for genetic engineering in agriculture. Despite the variability of the Cry proteins described so far, it is still necessary to look for toxins with a broad spectrum of action, since a significant number of pests are not controlled with the available Cry proteins. It is also important to provide alternatives to address the problem of insect resistance, which has already appeared with the use of formulations and in transgenic plants that express cry genes that code for insecticidal proteins. We report the characterization of a novel B. thuringiensis isolate native to Argentina (FCC7) toxic against lepidoptera and coleoptera insects. The strain shows a rounded crystal harboring mainly a protein of about 130 kDa. Through the whole-genome sequencing by Illumina Miseq 1500 platform we detected two crystal protein genes with cry8-like genes homology, three vegetative insecticidal proteins (Vips) genes and multiple virulence factors such as phospholipases, proteases, enhancins, chitinases, among others. The two cry8-like genes, homologues of Cry8Ac1 and Cry8Qa1 sequences with 73,4 % and 88,9 % of identity respectively, were cloned and expressed into the 4Q7/pSTAB system and the larvicidal activity were assayed against Spodoptera frugiperda and Tenebrio molitor. Two of the Vips genes were identified as Vip1-like with 74,9% and 70,3% identity respectively while the third Vip gene was 82% identical to Vip2 sequences.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Encuentro
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/138695
Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera based on its whole-genome analysis; XIV Encuentro Biólog@s En Red: 14 años por una ciencia hecha entre todes y para todes; Mar del Plata; Argentina; 2019; 42-42
1853-9998
CONICET Digital
CONICET
url http://hdl.handle.net/11336/138695
identifier_str_mv Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera based on its whole-genome analysis; XIV Encuentro Biólog@s En Red: 14 años por una ciencia hecha entre todes y para todes; Mar del Plata; Argentina; 2019; 42-42
1853-9998
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://xiv-ber-2019.blogspot.com/2019/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Asociación de Jóvenes Investigadores en Formación
publisher.none.fl_str_mv Asociación de Jóvenes Investigadores en Formación
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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