Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera base on its whole-genome analysis

Autores
Lazarte, Jorge Nicolás; Poliero, Aimará Ayelen; Berón, Corina Marta
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The insecticidal proteins of Bacillus thuringiensis are used as formulations of spore-crystal complexes and their genes have been incorporated into several crops, which has provided a model for genetic engineering in agriculture. Despite the variability of the Cry proteins described so far, it is still necessary to look for toxins with a broad spectrum of action, since a significant number of pests are not controlled with the available Cry proteins. It is also important to provide alternatives to address the problem of insect resistance, which has already appeared with the use of formulations and in transgenic plants that express cry genes that code for insecticidal proteins. We report the characterization of a novel B. thuringiensis isolate native to Argentina (FCC7) toxic against lepidoptera and coleoptera insects. The strain shows a rounded crystal harboring mainly a protein of about 130 kDa. Through the whole-genome sequencing by Illumina Miseq 1500 platform we detected two crystal protein genes with cry8-like genes homology, three vegetative insecticidal protein (Vip) genes and multiple virulence factors such as phospholipases, proteases, enhancins, chitinases, among others. The two cry8-like genes, homologous to the sequences Cry8Ac1 and Cry8Qa1 with 73,4 % and 88,9 % identity respectively, were cloned and expressed into the 4Q7/pSTAB system and the larvicidal activity were tested against Spodoptera frugiperda and Tenebrio molitor. Two of the Vip genes were identified as Vip1-like with an identity of 74,9% and 70,3% respectively while the third Vip gene was 82% identical to Vip2 sequences.
Fil: Lazarte, Jorge Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Poliero, Aimará Ayelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Berón, Corina Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
International Congress on Invertebrate Pathology and Microbial Control & 52nd Annual Meeting of the Society for Invertebrate Pathology & 17th Meeting of the IOBC‐WPRS Working Group "Microbial and Nematode Control of Invertebrate Pests"
Valencia
España
Societyfor Invertebrate Pathology
Organisation of IOBC
Materia
BIOLOGICAL CONTROL
GENOME
BACILLUS THURINGIENSIS
INSECT PESTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/131669
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/131669
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network_name_str CONICET Digital (CONICET)
spelling Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera base on its whole-genome analysisLazarte, Jorge NicolásPoliero, Aimará AyelenBerón, Corina MartaBIOLOGICAL CONTROLGENOMEBACILLUS THURINGIENSISINSECT PESTShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The insecticidal proteins of Bacillus thuringiensis are used as formulations of spore-crystal complexes and their genes have been incorporated into several crops, which has provided a model for genetic engineering in agriculture. Despite the variability of the Cry proteins described so far, it is still necessary to look for toxins with a broad spectrum of action, since a significant number of pests are not controlled with the available Cry proteins. It is also important to provide alternatives to address the problem of insect resistance, which has already appeared with the use of formulations and in transgenic plants that express cry genes that code for insecticidal proteins. We report the characterization of a novel B. thuringiensis isolate native to Argentina (FCC7) toxic against lepidoptera and coleoptera insects. The strain shows a rounded crystal harboring mainly a protein of about 130 kDa. Through the whole-genome sequencing by Illumina Miseq 1500 platform we detected two crystal protein genes with cry8-like genes homology, three vegetative insecticidal protein (Vip) genes and multiple virulence factors such as phospholipases, proteases, enhancins, chitinases, among others. The two cry8-like genes, homologous to the sequences Cry8Ac1 and Cry8Qa1 with 73,4 % and 88,9 % identity respectively, were cloned and expressed into the 4Q7/pSTAB system and the larvicidal activity were tested against Spodoptera frugiperda and Tenebrio molitor. Two of the Vip genes were identified as Vip1-like with an identity of 74,9% and 70,3% respectively while the third Vip gene was 82% identical to Vip2 sequences.Fil: Lazarte, Jorge Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaFil: Poliero, Aimará Ayelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaFil: Berón, Corina Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaInternational Congress on Invertebrate Pathology and Microbial Control & 52nd Annual Meeting of the Society for Invertebrate Pathology & 17th Meeting of the IOBC‐WPRS Working Group "Microbial and Nematode Control of Invertebrate Pests"ValenciaEspañaSocietyfor Invertebrate PathologyOrganisation of IOBCSociety for Invertebrate Pathology2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/131669Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera base on its whole-genome analysis; International Congress on Invertebrate Pathology and Microbial Control & 52nd Annual Meeting of the Society for Invertebrate Pathology & 17th Meeting of the IOBC‐WPRS Working Group "Microbial and Nematode Control of Invertebrate Pests"; Valencia; España; 2019; 96-96CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sipweb.org/docs/abstract-program2019Valencia.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:34:16Zoai:ri.conicet.gov.ar:11336/131669instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:34:16.588CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera base on its whole-genome analysis
title Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera base on its whole-genome analysis
spellingShingle Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera base on its whole-genome analysis
Lazarte, Jorge Nicolás
BIOLOGICAL CONTROL
GENOME
BACILLUS THURINGIENSIS
INSECT PESTS
title_short Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera base on its whole-genome analysis
title_full Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera base on its whole-genome analysis
title_fullStr Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera base on its whole-genome analysis
title_full_unstemmed Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera base on its whole-genome analysis
title_sort Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera base on its whole-genome analysis
dc.creator.none.fl_str_mv Lazarte, Jorge Nicolás
Poliero, Aimará Ayelen
Berón, Corina Marta
author Lazarte, Jorge Nicolás
author_facet Lazarte, Jorge Nicolás
Poliero, Aimará Ayelen
Berón, Corina Marta
author_role author
author2 Poliero, Aimará Ayelen
Berón, Corina Marta
author2_role author
author
dc.subject.none.fl_str_mv BIOLOGICAL CONTROL
GENOME
BACILLUS THURINGIENSIS
INSECT PESTS
topic BIOLOGICAL CONTROL
GENOME
BACILLUS THURINGIENSIS
INSECT PESTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The insecticidal proteins of Bacillus thuringiensis are used as formulations of spore-crystal complexes and their genes have been incorporated into several crops, which has provided a model for genetic engineering in agriculture. Despite the variability of the Cry proteins described so far, it is still necessary to look for toxins with a broad spectrum of action, since a significant number of pests are not controlled with the available Cry proteins. It is also important to provide alternatives to address the problem of insect resistance, which has already appeared with the use of formulations and in transgenic plants that express cry genes that code for insecticidal proteins. We report the characterization of a novel B. thuringiensis isolate native to Argentina (FCC7) toxic against lepidoptera and coleoptera insects. The strain shows a rounded crystal harboring mainly a protein of about 130 kDa. Through the whole-genome sequencing by Illumina Miseq 1500 platform we detected two crystal protein genes with cry8-like genes homology, three vegetative insecticidal protein (Vip) genes and multiple virulence factors such as phospholipases, proteases, enhancins, chitinases, among others. The two cry8-like genes, homologous to the sequences Cry8Ac1 and Cry8Qa1 with 73,4 % and 88,9 % identity respectively, were cloned and expressed into the 4Q7/pSTAB system and the larvicidal activity were tested against Spodoptera frugiperda and Tenebrio molitor. Two of the Vip genes were identified as Vip1-like with an identity of 74,9% and 70,3% respectively while the third Vip gene was 82% identical to Vip2 sequences.
Fil: Lazarte, Jorge Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Poliero, Aimará Ayelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Berón, Corina Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
International Congress on Invertebrate Pathology and Microbial Control & 52nd Annual Meeting of the Society for Invertebrate Pathology & 17th Meeting of the IOBC‐WPRS Working Group "Microbial and Nematode Control of Invertebrate Pests"
Valencia
España
Societyfor Invertebrate Pathology
Organisation of IOBC
description The insecticidal proteins of Bacillus thuringiensis are used as formulations of spore-crystal complexes and their genes have been incorporated into several crops, which has provided a model for genetic engineering in agriculture. Despite the variability of the Cry proteins described so far, it is still necessary to look for toxins with a broad spectrum of action, since a significant number of pests are not controlled with the available Cry proteins. It is also important to provide alternatives to address the problem of insect resistance, which has already appeared with the use of formulations and in transgenic plants that express cry genes that code for insecticidal proteins. We report the characterization of a novel B. thuringiensis isolate native to Argentina (FCC7) toxic against lepidoptera and coleoptera insects. The strain shows a rounded crystal harboring mainly a protein of about 130 kDa. Through the whole-genome sequencing by Illumina Miseq 1500 platform we detected two crystal protein genes with cry8-like genes homology, three vegetative insecticidal protein (Vip) genes and multiple virulence factors such as phospholipases, proteases, enhancins, chitinases, among others. The two cry8-like genes, homologous to the sequences Cry8Ac1 and Cry8Qa1 with 73,4 % and 88,9 % identity respectively, were cloned and expressed into the 4Q7/pSTAB system and the larvicidal activity were tested against Spodoptera frugiperda and Tenebrio molitor. Two of the Vip genes were identified as Vip1-like with an identity of 74,9% and 70,3% respectively while the third Vip gene was 82% identical to Vip2 sequences.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/131669
Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera base on its whole-genome analysis; International Congress on Invertebrate Pathology and Microbial Control & 52nd Annual Meeting of the Society for Invertebrate Pathology & 17th Meeting of the IOBC‐WPRS Working Group "Microbial and Nematode Control of Invertebrate Pests"; Valencia; España; 2019; 96-96
CONICET Digital
CONICET
url http://hdl.handle.net/11336/131669
identifier_str_mv Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera base on its whole-genome analysis; International Congress on Invertebrate Pathology and Microbial Control & 52nd Annual Meeting of the Society for Invertebrate Pathology & 17th Meeting of the IOBC‐WPRS Working Group "Microbial and Nematode Control of Invertebrate Pests"; Valencia; España; 2019; 96-96
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sipweb.org/docs/abstract-program2019Valencia.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Society for Invertebrate Pathology
publisher.none.fl_str_mv Society for Invertebrate Pathology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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