Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera base on its whole-genome analysis
- Autores
- Lazarte, Jorge Nicolás; Poliero, Aimará Ayelen; Berón, Corina Marta
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- The insecticidal proteins of Bacillus thuringiensis are used as formulations of spore-crystal complexes and their genes have been incorporated into several crops, which has provided a model for genetic engineering in agriculture. Despite the variability of the Cry proteins described so far, it is still necessary to look for toxins with a broad spectrum of action, since a significant number of pests are not controlled with the available Cry proteins. It is also important to provide alternatives to address the problem of insect resistance, which has already appeared with the use of formulations and in transgenic plants that express cry genes that code for insecticidal proteins. We report the characterization of a novel B. thuringiensis isolate native to Argentina (FCC7) toxic against lepidoptera and coleoptera insects. The strain shows a rounded crystal harboring mainly a protein of about 130 kDa. Through the whole-genome sequencing by Illumina Miseq 1500 platform we detected two crystal protein genes with cry8-like genes homology, three vegetative insecticidal protein (Vip) genes and multiple virulence factors such as phospholipases, proteases, enhancins, chitinases, among others. The two cry8-like genes, homologous to the sequences Cry8Ac1 and Cry8Qa1 with 73,4 % and 88,9 % identity respectively, were cloned and expressed into the 4Q7/pSTAB system and the larvicidal activity were tested against Spodoptera frugiperda and Tenebrio molitor. Two of the Vip genes were identified as Vip1-like with an identity of 74,9% and 70,3% respectively while the third Vip gene was 82% identical to Vip2 sequences.
Fil: Lazarte, Jorge Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Poliero, Aimará Ayelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
Fil: Berón, Corina Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
International Congress on Invertebrate Pathology and Microbial Control & 52nd Annual Meeting of the Society for Invertebrate Pathology & 17th Meeting of the IOBC‐WPRS Working Group "Microbial and Nematode Control of Invertebrate Pests"
Valencia
España
Societyfor Invertebrate Pathology
Organisation of IOBC - Materia
-
BIOLOGICAL CONTROL
GENOME
BACILLUS THURINGIENSIS
INSECT PESTS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/131669
Ver los metadatos del registro completo
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Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera base on its whole-genome analysisLazarte, Jorge NicolásPoliero, Aimará AyelenBerón, Corina MartaBIOLOGICAL CONTROLGENOMEBACILLUS THURINGIENSISINSECT PESTShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The insecticidal proteins of Bacillus thuringiensis are used as formulations of spore-crystal complexes and their genes have been incorporated into several crops, which has provided a model for genetic engineering in agriculture. Despite the variability of the Cry proteins described so far, it is still necessary to look for toxins with a broad spectrum of action, since a significant number of pests are not controlled with the available Cry proteins. It is also important to provide alternatives to address the problem of insect resistance, which has already appeared with the use of formulations and in transgenic plants that express cry genes that code for insecticidal proteins. We report the characterization of a novel B. thuringiensis isolate native to Argentina (FCC7) toxic against lepidoptera and coleoptera insects. The strain shows a rounded crystal harboring mainly a protein of about 130 kDa. Through the whole-genome sequencing by Illumina Miseq 1500 platform we detected two crystal protein genes with cry8-like genes homology, three vegetative insecticidal protein (Vip) genes and multiple virulence factors such as phospholipases, proteases, enhancins, chitinases, among others. The two cry8-like genes, homologous to the sequences Cry8Ac1 and Cry8Qa1 with 73,4 % and 88,9 % identity respectively, were cloned and expressed into the 4Q7/pSTAB system and the larvicidal activity were tested against Spodoptera frugiperda and Tenebrio molitor. Two of the Vip genes were identified as Vip1-like with an identity of 74,9% and 70,3% respectively while the third Vip gene was 82% identical to Vip2 sequences.Fil: Lazarte, Jorge Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaFil: Poliero, Aimará Ayelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaFil: Berón, Corina Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaInternational Congress on Invertebrate Pathology and Microbial Control & 52nd Annual Meeting of the Society for Invertebrate Pathology & 17th Meeting of the IOBC‐WPRS Working Group "Microbial and Nematode Control of Invertebrate Pests"ValenciaEspañaSocietyfor Invertebrate PathologyOrganisation of IOBCSociety for Invertebrate Pathology2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/131669Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera base on its whole-genome analysis; International Congress on Invertebrate Pathology and Microbial Control & 52nd Annual Meeting of the Society for Invertebrate Pathology & 17th Meeting of the IOBC‐WPRS Working Group "Microbial and Nematode Control of Invertebrate Pests"; Valencia; España; 2019; 96-96CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sipweb.org/docs/abstract-program2019Valencia.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:04:31Zoai:ri.conicet.gov.ar:11336/131669instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:04:31.449CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera base on its whole-genome analysis |
title |
Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera base on its whole-genome analysis |
spellingShingle |
Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera base on its whole-genome analysis Lazarte, Jorge Nicolás BIOLOGICAL CONTROL GENOME BACILLUS THURINGIENSIS INSECT PESTS |
title_short |
Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera base on its whole-genome analysis |
title_full |
Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera base on its whole-genome analysis |
title_fullStr |
Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera base on its whole-genome analysis |
title_full_unstemmed |
Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera base on its whole-genome analysis |
title_sort |
Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera base on its whole-genome analysis |
dc.creator.none.fl_str_mv |
Lazarte, Jorge Nicolás Poliero, Aimará Ayelen Berón, Corina Marta |
author |
Lazarte, Jorge Nicolás |
author_facet |
Lazarte, Jorge Nicolás Poliero, Aimará Ayelen Berón, Corina Marta |
author_role |
author |
author2 |
Poliero, Aimará Ayelen Berón, Corina Marta |
author2_role |
author author |
dc.subject.none.fl_str_mv |
BIOLOGICAL CONTROL GENOME BACILLUS THURINGIENSIS INSECT PESTS |
topic |
BIOLOGICAL CONTROL GENOME BACILLUS THURINGIENSIS INSECT PESTS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The insecticidal proteins of Bacillus thuringiensis are used as formulations of spore-crystal complexes and their genes have been incorporated into several crops, which has provided a model for genetic engineering in agriculture. Despite the variability of the Cry proteins described so far, it is still necessary to look for toxins with a broad spectrum of action, since a significant number of pests are not controlled with the available Cry proteins. It is also important to provide alternatives to address the problem of insect resistance, which has already appeared with the use of formulations and in transgenic plants that express cry genes that code for insecticidal proteins. We report the characterization of a novel B. thuringiensis isolate native to Argentina (FCC7) toxic against lepidoptera and coleoptera insects. The strain shows a rounded crystal harboring mainly a protein of about 130 kDa. Through the whole-genome sequencing by Illumina Miseq 1500 platform we detected two crystal protein genes with cry8-like genes homology, three vegetative insecticidal protein (Vip) genes and multiple virulence factors such as phospholipases, proteases, enhancins, chitinases, among others. The two cry8-like genes, homologous to the sequences Cry8Ac1 and Cry8Qa1 with 73,4 % and 88,9 % identity respectively, were cloned and expressed into the 4Q7/pSTAB system and the larvicidal activity were tested against Spodoptera frugiperda and Tenebrio molitor. Two of the Vip genes were identified as Vip1-like with an identity of 74,9% and 70,3% respectively while the third Vip gene was 82% identical to Vip2 sequences. Fil: Lazarte, Jorge Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina Fil: Poliero, Aimará Ayelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina Fil: Berón, Corina Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina International Congress on Invertebrate Pathology and Microbial Control & 52nd Annual Meeting of the Society for Invertebrate Pathology & 17th Meeting of the IOBC‐WPRS Working Group "Microbial and Nematode Control of Invertebrate Pests" Valencia España Societyfor Invertebrate Pathology Organisation of IOBC |
description |
The insecticidal proteins of Bacillus thuringiensis are used as formulations of spore-crystal complexes and their genes have been incorporated into several crops, which has provided a model for genetic engineering in agriculture. Despite the variability of the Cry proteins described so far, it is still necessary to look for toxins with a broad spectrum of action, since a significant number of pests are not controlled with the available Cry proteins. It is also important to provide alternatives to address the problem of insect resistance, which has already appeared with the use of formulations and in transgenic plants that express cry genes that code for insecticidal proteins. We report the characterization of a novel B. thuringiensis isolate native to Argentina (FCC7) toxic against lepidoptera and coleoptera insects. The strain shows a rounded crystal harboring mainly a protein of about 130 kDa. Through the whole-genome sequencing by Illumina Miseq 1500 platform we detected two crystal protein genes with cry8-like genes homology, three vegetative insecticidal protein (Vip) genes and multiple virulence factors such as phospholipases, proteases, enhancins, chitinases, among others. The two cry8-like genes, homologous to the sequences Cry8Ac1 and Cry8Qa1 with 73,4 % and 88,9 % identity respectively, were cloned and expressed into the 4Q7/pSTAB system and the larvicidal activity were tested against Spodoptera frugiperda and Tenebrio molitor. Two of the Vip genes were identified as Vip1-like with an identity of 74,9% and 70,3% respectively while the third Vip gene was 82% identical to Vip2 sequences. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Congreso Book http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/131669 Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera base on its whole-genome analysis; International Congress on Invertebrate Pathology and Microbial Control & 52nd Annual Meeting of the Society for Invertebrate Pathology & 17th Meeting of the IOBC‐WPRS Working Group "Microbial and Nematode Control of Invertebrate Pests"; Valencia; España; 2019; 96-96 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/131669 |
identifier_str_mv |
Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera base on its whole-genome analysis; International Congress on Invertebrate Pathology and Microbial Control & 52nd Annual Meeting of the Society for Invertebrate Pathology & 17th Meeting of the IOBC‐WPRS Working Group "Microbial and Nematode Control of Invertebrate Pests"; Valencia; España; 2019; 96-96 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.sipweb.org/docs/abstract-program2019Valencia.pdf |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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application/pdf application/pdf application/pdf |
dc.coverage.none.fl_str_mv |
Internacional |
dc.publisher.none.fl_str_mv |
Society for Invertebrate Pathology |
publisher.none.fl_str_mv |
Society for Invertebrate Pathology |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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