Highlighting Astyanax species diversity through DNA barcoding

Autores
Rossini, Bruno César; Oliveira, Carlos Alexandre Miranda; Melo, Filipe Augusto Gonçalves De; Bertaco, Vinicius De Araú Jo; Díaz de Astarloa, Juan Martín; Rosso, Juan Jose; Foresti, Fausto; Claudio, Oliveira
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
DNA barcoding has been used extensively to solve taxonomic questions and identify new species. Neotropical fishes are found in a wide variety of shapes and sizes, with a large number of species yet to be described, many of which are very difficult to identify. Characidae is the most species-rich family of the Characiformes, and many of its genera are affected by taxonomic uncertainties, including the widely-distributed, species-rich genus Astyanax. In this study, we present an extensive analysis of Astyanax covering almost its entire area of occurrence, based on DNA barcoding. The use of different approaches (ABGD, GMYC and BIN) to the clustering of the sequences revealed ample consistency in the results obtained by the initial cutoff value of 2% divergence for putative species in the Neighbor-Joining analysis using the Kimura-2-parameter model. The results indicate the existence of five Astyanax lineages. Some groups, such as that composed by the trans- Andean forms, are mostly composed of well-defined species, and in others a number of nominal species are clustered together, hampering the delimitation of species, which in many cases proved impossible. The results confirm the extreme complexity of the systematics of the genus Astyanax and show that DNA barcoding can be an useful tool to address these complexes questions.
Fil: Rossini, Bruno César. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Oliveira, Carlos Alexandre Miranda. Universidade Estadual de Maringá; Brasil
Fil: Melo, Filipe Augusto Gonçalves De. Universidade Estadual do Piauí; Brasil
Fil: Bertaco, Vinicius De Araú Jo. Fundação Zoobotânica do Rio Grande do Sul. Museu de Ciências Naturais; Brasil
Fil: Díaz de Astarloa, Juan Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Rosso, Juan Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Foresti, Fausto. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Claudio, Oliveira. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Materia
Astyanax
Diversity
DNA Barcoding
South America
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/62035
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Highlighting Astyanax species diversity through DNA barcodingRossini, Bruno CésarOliveira, Carlos Alexandre MirandaMelo, Filipe Augusto Gonçalves DeBertaco, Vinicius De Araú JoDíaz de Astarloa, Juan MartínRosso, Juan JoseForesti, FaustoClaudio, OliveiraAstyanaxDiversityDNA BarcodingSouth Americahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1DNA barcoding has been used extensively to solve taxonomic questions and identify new species. Neotropical fishes are found in a wide variety of shapes and sizes, with a large number of species yet to be described, many of which are very difficult to identify. Characidae is the most species-rich family of the Characiformes, and many of its genera are affected by taxonomic uncertainties, including the widely-distributed, species-rich genus Astyanax. In this study, we present an extensive analysis of Astyanax covering almost its entire area of occurrence, based on DNA barcoding. The use of different approaches (ABGD, GMYC and BIN) to the clustering of the sequences revealed ample consistency in the results obtained by the initial cutoff value of 2% divergence for putative species in the Neighbor-Joining analysis using the Kimura-2-parameter model. The results indicate the existence of five Astyanax lineages. Some groups, such as that composed by the trans- Andean forms, are mostly composed of well-defined species, and in others a number of nominal species are clustered together, hampering the delimitation of species, which in many cases proved impossible. The results confirm the extreme complexity of the systematics of the genus Astyanax and show that DNA barcoding can be an useful tool to address these complexes questions.Fil: Rossini, Bruno César. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Oliveira, Carlos Alexandre Miranda. Universidade Estadual de Maringá; BrasilFil: Melo, Filipe Augusto Gonçalves De. Universidade Estadual do Piauí; BrasilFil: Bertaco, Vinicius De Araú Jo. Fundação Zoobotânica do Rio Grande do Sul. Museu de Ciências Naturais; BrasilFil: Díaz de Astarloa, Juan Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Rosso, Juan Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Foresti, Fausto. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Claudio, Oliveira. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilPublic Library of Science2016-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/62035Rossini, Bruno César; Oliveira, Carlos Alexandre Miranda; Melo, Filipe Augusto Gonçalves De; Bertaco, Vinicius De Araú Jo ; Díaz de Astarloa, Juan Martín; et al.; Highlighting Astyanax species diversity through DNA barcoding; Public Library of Science; Plos One; 11; 12; 12-2016; 1-20; e01672031932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0167203info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0167203info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:12:01Zoai:ri.conicet.gov.ar:11336/62035instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:12:01.925CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Highlighting Astyanax species diversity through DNA barcoding
title Highlighting Astyanax species diversity through DNA barcoding
spellingShingle Highlighting Astyanax species diversity through DNA barcoding
Rossini, Bruno César
Astyanax
Diversity
DNA Barcoding
South America
title_short Highlighting Astyanax species diversity through DNA barcoding
title_full Highlighting Astyanax species diversity through DNA barcoding
title_fullStr Highlighting Astyanax species diversity through DNA barcoding
title_full_unstemmed Highlighting Astyanax species diversity through DNA barcoding
title_sort Highlighting Astyanax species diversity through DNA barcoding
dc.creator.none.fl_str_mv Rossini, Bruno César
Oliveira, Carlos Alexandre Miranda
Melo, Filipe Augusto Gonçalves De
Bertaco, Vinicius De Araú Jo
Díaz de Astarloa, Juan Martín
Rosso, Juan Jose
Foresti, Fausto
Claudio, Oliveira
author Rossini, Bruno César
author_facet Rossini, Bruno César
Oliveira, Carlos Alexandre Miranda
Melo, Filipe Augusto Gonçalves De
Bertaco, Vinicius De Araú Jo
Díaz de Astarloa, Juan Martín
Rosso, Juan Jose
Foresti, Fausto
Claudio, Oliveira
author_role author
author2 Oliveira, Carlos Alexandre Miranda
Melo, Filipe Augusto Gonçalves De
Bertaco, Vinicius De Araú Jo
Díaz de Astarloa, Juan Martín
Rosso, Juan Jose
Foresti, Fausto
Claudio, Oliveira
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Astyanax
Diversity
DNA Barcoding
South America
topic Astyanax
Diversity
DNA Barcoding
South America
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv DNA barcoding has been used extensively to solve taxonomic questions and identify new species. Neotropical fishes are found in a wide variety of shapes and sizes, with a large number of species yet to be described, many of which are very difficult to identify. Characidae is the most species-rich family of the Characiformes, and many of its genera are affected by taxonomic uncertainties, including the widely-distributed, species-rich genus Astyanax. In this study, we present an extensive analysis of Astyanax covering almost its entire area of occurrence, based on DNA barcoding. The use of different approaches (ABGD, GMYC and BIN) to the clustering of the sequences revealed ample consistency in the results obtained by the initial cutoff value of 2% divergence for putative species in the Neighbor-Joining analysis using the Kimura-2-parameter model. The results indicate the existence of five Astyanax lineages. Some groups, such as that composed by the trans- Andean forms, are mostly composed of well-defined species, and in others a number of nominal species are clustered together, hampering the delimitation of species, which in many cases proved impossible. The results confirm the extreme complexity of the systematics of the genus Astyanax and show that DNA barcoding can be an useful tool to address these complexes questions.
Fil: Rossini, Bruno César. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Oliveira, Carlos Alexandre Miranda. Universidade Estadual de Maringá; Brasil
Fil: Melo, Filipe Augusto Gonçalves De. Universidade Estadual do Piauí; Brasil
Fil: Bertaco, Vinicius De Araú Jo. Fundação Zoobotânica do Rio Grande do Sul. Museu de Ciências Naturais; Brasil
Fil: Díaz de Astarloa, Juan Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Rosso, Juan Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencia Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Foresti, Fausto. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Claudio, Oliveira. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
description DNA barcoding has been used extensively to solve taxonomic questions and identify new species. Neotropical fishes are found in a wide variety of shapes and sizes, with a large number of species yet to be described, many of which are very difficult to identify. Characidae is the most species-rich family of the Characiformes, and many of its genera are affected by taxonomic uncertainties, including the widely-distributed, species-rich genus Astyanax. In this study, we present an extensive analysis of Astyanax covering almost its entire area of occurrence, based on DNA barcoding. The use of different approaches (ABGD, GMYC and BIN) to the clustering of the sequences revealed ample consistency in the results obtained by the initial cutoff value of 2% divergence for putative species in the Neighbor-Joining analysis using the Kimura-2-parameter model. The results indicate the existence of five Astyanax lineages. Some groups, such as that composed by the trans- Andean forms, are mostly composed of well-defined species, and in others a number of nominal species are clustered together, hampering the delimitation of species, which in many cases proved impossible. The results confirm the extreme complexity of the systematics of the genus Astyanax and show that DNA barcoding can be an useful tool to address these complexes questions.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/62035
Rossini, Bruno César; Oliveira, Carlos Alexandre Miranda; Melo, Filipe Augusto Gonçalves De; Bertaco, Vinicius De Araú Jo ; Díaz de Astarloa, Juan Martín; et al.; Highlighting Astyanax species diversity through DNA barcoding; Public Library of Science; Plos One; 11; 12; 12-2016; 1-20; e0167203
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/62035
identifier_str_mv Rossini, Bruno César; Oliveira, Carlos Alexandre Miranda; Melo, Filipe Augusto Gonçalves De; Bertaco, Vinicius De Araú Jo ; Díaz de Astarloa, Juan Martín; et al.; Highlighting Astyanax species diversity through DNA barcoding; Public Library of Science; Plos One; 11; 12; 12-2016; 1-20; e0167203
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0167203
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0167203
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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