DNA barcoding supports identification of Malacobdella species (Nemertea: Hoplonemertea)

Autores
Fernandez Alfaya, Jose Elias; Bigatti, Gregorio; Kajihara, Hiroshi; Strand, Malin; Sundberg, Per; Machordom, Annie
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Nemerteans of the genus Malacobdella live inside of the mantle cavity of marine bivalves. The genus currently contains only six species, five of which are host-specific and usually found in a single host species, while the sixth species, M. grossa, has a wide host range and has been found in 27 different bivalve species to date. The main challenge of Malacobdella species identification resides in the similarity of the external morphology between species (terminal sucker, gut undulations number, anus position and gonad colouration), and thus, the illustrations provided in the original descriptions do not allow reliable identification. In this article, we analyse the relationships among three species of Malacobdella: M. arrokeana, M. japonica and M. grossa, adding new data for the M. grossa and reporting the first for M. japonica, analysing 658 base pairs of the mitochondrial cytochrome c oxidase subunit I gene (COI). Based on these analyses, we present and discuss the potential of DNA barcoding for Malacobdella species identification. Results: Sixty-four DNA barcoding fragments of the mitochondrial COI gene from three different Malacobdella species (M. arrokeana, M. japonica and M. grossa) are analysed (24 of them newly sequenced for this study, along with four outgroup specimens) and used to delineate species. Divergences, measured as uncorrected differences, between the three species were M. arrokeana-M. grossa 11.73%, M. arrokeana-M. japonica 10.62% and M. grossa-M. japonica 10.97%. The mean intraspecific divergence within the ingroup species showed a patent gap with respect to the interspecific ones: 0.18% for M. arrokeana, 0.13% for M. grossa and 0.02% for M. japonica (ranges from 0 to 0.91%). Conclusions: We conclude that there is a clear correspondence between the molecular data and distinguishing morphological characters. Our results thus indicate that some morphological characters are useful for species identification and support the potential of DNA barcoding for species identification in a taxonomic group with subtle morphological external differences.
Fil: Fernandez Alfaya, Jose Elias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biología de Organismos Marinos; Argentina. Universidad Nacional de la Patagonia; Argentina
Fil: Bigatti, Gregorio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biología de Organismos Marinos; Argentina. Universidad Nacional de la Patagonia; Argentina
Fil: Kajihara, Hiroshi. Hokkaido University; Japón
Fil: Strand, Malin. The Swedish Species Information Centre SLU; Suecia
Fil: Sundberg, Per. University of Gothenburg; Suecia
Fil: Machordom, Annie. Consejo Superior de Investigaciones Cientificas. Museo Nacional de Ciencias Naturales; España
Materia
DNA BARCODING
COI GENE
BIVALVIA
ENTOCOMMENSAL NEMERTEAN
MALACOBDELLA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/27953
Acceder
id CONICETDig_23452b811afe1b06b0e583acfd23415d
oai_identifier_str oai:ri.conicet.gov.ar:11336/27953
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling DNA barcoding supports identification of Malacobdella species (Nemertea: Hoplonemertea)Fernandez Alfaya, Jose EliasBigatti, GregorioKajihara, HiroshiStrand, MalinSundberg, PerMachordom, AnnieDNA BARCODINGCOI GENEBIVALVIAENTOCOMMENSAL NEMERTEANMALACOBDELLAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Nemerteans of the genus Malacobdella live inside of the mantle cavity of marine bivalves. The genus currently contains only six species, five of which are host-specific and usually found in a single host species, while the sixth species, M. grossa, has a wide host range and has been found in 27 different bivalve species to date. The main challenge of Malacobdella species identification resides in the similarity of the external morphology between species (terminal sucker, gut undulations number, anus position and gonad colouration), and thus, the illustrations provided in the original descriptions do not allow reliable identification. In this article, we analyse the relationships among three species of Malacobdella: M. arrokeana, M. japonica and M. grossa, adding new data for the M. grossa and reporting the first for M. japonica, analysing 658 base pairs of the mitochondrial cytochrome c oxidase subunit I gene (COI). Based on these analyses, we present and discuss the potential of DNA barcoding for Malacobdella species identification. Results: Sixty-four DNA barcoding fragments of the mitochondrial COI gene from three different Malacobdella species (M. arrokeana, M. japonica and M. grossa) are analysed (24 of them newly sequenced for this study, along with four outgroup specimens) and used to delineate species. Divergences, measured as uncorrected differences, between the three species were M. arrokeana-M. grossa 11.73%, M. arrokeana-M. japonica 10.62% and M. grossa-M. japonica 10.97%. The mean intraspecific divergence within the ingroup species showed a patent gap with respect to the interspecific ones: 0.18% for M. arrokeana, 0.13% for M. grossa and 0.02% for M. japonica (ranges from 0 to 0.91%). Conclusions: We conclude that there is a clear correspondence between the molecular data and distinguishing morphological characters. Our results thus indicate that some morphological characters are useful for species identification and support the potential of DNA barcoding for species identification in a taxonomic group with subtle morphological external differences.Fil: Fernandez Alfaya, Jose Elias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biología de Organismos Marinos; Argentina. Universidad Nacional de la Patagonia; ArgentinaFil: Bigatti, Gregorio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biología de Organismos Marinos; Argentina. Universidad Nacional de la Patagonia; ArgentinaFil: Kajihara, Hiroshi. Hokkaido University; JapónFil: Strand, Malin. The Swedish Species Information Centre SLU; SueciaFil: Sundberg, Per. University of Gothenburg; SueciaFil: Machordom, Annie. Consejo Superior de Investigaciones Cientificas. Museo Nacional de Ciencias Naturales; EspañaSpringer2015-01-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/27953Fernandez Alfaya, Jose Elias; Bigatti, Gregorio; Kajihara, Hiroshi; Strand, Malin; Sundberg, Per; et al.; DNA barcoding supports identification of Malacobdella species (Nemertea: Hoplonemertea); Springer; Zoological Studies; 54; 10; 10-1-2015; 1-91810-522XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186/s40555-014-0086-3info:eu-repo/semantics/altIdentifier/url/http://zoolstud.sinica.edu.tw/54.htminfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:34Zoai:ri.conicet.gov.ar:11336/27953instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:35.127CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv DNA barcoding supports identification of Malacobdella species (Nemertea: Hoplonemertea)
title DNA barcoding supports identification of Malacobdella species (Nemertea: Hoplonemertea)
spellingShingle DNA barcoding supports identification of Malacobdella species (Nemertea: Hoplonemertea)
Fernandez Alfaya, Jose Elias
DNA BARCODING
COI GENE
BIVALVIA
ENTOCOMMENSAL NEMERTEAN
MALACOBDELLA
title_short DNA barcoding supports identification of Malacobdella species (Nemertea: Hoplonemertea)
title_full DNA barcoding supports identification of Malacobdella species (Nemertea: Hoplonemertea)
title_fullStr DNA barcoding supports identification of Malacobdella species (Nemertea: Hoplonemertea)
title_full_unstemmed DNA barcoding supports identification of Malacobdella species (Nemertea: Hoplonemertea)
title_sort DNA barcoding supports identification of Malacobdella species (Nemertea: Hoplonemertea)
dc.creator.none.fl_str_mv Fernandez Alfaya, Jose Elias
Bigatti, Gregorio
Kajihara, Hiroshi
Strand, Malin
Sundberg, Per
Machordom, Annie
author Fernandez Alfaya, Jose Elias
author_facet Fernandez Alfaya, Jose Elias
Bigatti, Gregorio
Kajihara, Hiroshi
Strand, Malin
Sundberg, Per
Machordom, Annie
author_role author
author2 Bigatti, Gregorio
Kajihara, Hiroshi
Strand, Malin
Sundberg, Per
Machordom, Annie
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv DNA BARCODING
COI GENE
BIVALVIA
ENTOCOMMENSAL NEMERTEAN
MALACOBDELLA
topic DNA BARCODING
COI GENE
BIVALVIA
ENTOCOMMENSAL NEMERTEAN
MALACOBDELLA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Nemerteans of the genus Malacobdella live inside of the mantle cavity of marine bivalves. The genus currently contains only six species, five of which are host-specific and usually found in a single host species, while the sixth species, M. grossa, has a wide host range and has been found in 27 different bivalve species to date. The main challenge of Malacobdella species identification resides in the similarity of the external morphology between species (terminal sucker, gut undulations number, anus position and gonad colouration), and thus, the illustrations provided in the original descriptions do not allow reliable identification. In this article, we analyse the relationships among three species of Malacobdella: M. arrokeana, M. japonica and M. grossa, adding new data for the M. grossa and reporting the first for M. japonica, analysing 658 base pairs of the mitochondrial cytochrome c oxidase subunit I gene (COI). Based on these analyses, we present and discuss the potential of DNA barcoding for Malacobdella species identification. Results: Sixty-four DNA barcoding fragments of the mitochondrial COI gene from three different Malacobdella species (M. arrokeana, M. japonica and M. grossa) are analysed (24 of them newly sequenced for this study, along with four outgroup specimens) and used to delineate species. Divergences, measured as uncorrected differences, between the three species were M. arrokeana-M. grossa 11.73%, M. arrokeana-M. japonica 10.62% and M. grossa-M. japonica 10.97%. The mean intraspecific divergence within the ingroup species showed a patent gap with respect to the interspecific ones: 0.18% for M. arrokeana, 0.13% for M. grossa and 0.02% for M. japonica (ranges from 0 to 0.91%). Conclusions: We conclude that there is a clear correspondence between the molecular data and distinguishing morphological characters. Our results thus indicate that some morphological characters are useful for species identification and support the potential of DNA barcoding for species identification in a taxonomic group with subtle morphological external differences.
Fil: Fernandez Alfaya, Jose Elias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biología de Organismos Marinos; Argentina. Universidad Nacional de la Patagonia; Argentina
Fil: Bigatti, Gregorio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biología de Organismos Marinos; Argentina. Universidad Nacional de la Patagonia; Argentina
Fil: Kajihara, Hiroshi. Hokkaido University; Japón
Fil: Strand, Malin. The Swedish Species Information Centre SLU; Suecia
Fil: Sundberg, Per. University of Gothenburg; Suecia
Fil: Machordom, Annie. Consejo Superior de Investigaciones Cientificas. Museo Nacional de Ciencias Naturales; España
description Background: Nemerteans of the genus Malacobdella live inside of the mantle cavity of marine bivalves. The genus currently contains only six species, five of which are host-specific and usually found in a single host species, while the sixth species, M. grossa, has a wide host range and has been found in 27 different bivalve species to date. The main challenge of Malacobdella species identification resides in the similarity of the external morphology between species (terminal sucker, gut undulations number, anus position and gonad colouration), and thus, the illustrations provided in the original descriptions do not allow reliable identification. In this article, we analyse the relationships among three species of Malacobdella: M. arrokeana, M. japonica and M. grossa, adding new data for the M. grossa and reporting the first for M. japonica, analysing 658 base pairs of the mitochondrial cytochrome c oxidase subunit I gene (COI). Based on these analyses, we present and discuss the potential of DNA barcoding for Malacobdella species identification. Results: Sixty-four DNA barcoding fragments of the mitochondrial COI gene from three different Malacobdella species (M. arrokeana, M. japonica and M. grossa) are analysed (24 of them newly sequenced for this study, along with four outgroup specimens) and used to delineate species. Divergences, measured as uncorrected differences, between the three species were M. arrokeana-M. grossa 11.73%, M. arrokeana-M. japonica 10.62% and M. grossa-M. japonica 10.97%. The mean intraspecific divergence within the ingroup species showed a patent gap with respect to the interspecific ones: 0.18% for M. arrokeana, 0.13% for M. grossa and 0.02% for M. japonica (ranges from 0 to 0.91%). Conclusions: We conclude that there is a clear correspondence between the molecular data and distinguishing morphological characters. Our results thus indicate that some morphological characters are useful for species identification and support the potential of DNA barcoding for species identification in a taxonomic group with subtle morphological external differences.
publishDate 2015
dc.date.none.fl_str_mv 2015-01-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/27953
Fernandez Alfaya, Jose Elias; Bigatti, Gregorio; Kajihara, Hiroshi; Strand, Malin; Sundberg, Per; et al.; DNA barcoding supports identification of Malacobdella species (Nemertea: Hoplonemertea); Springer; Zoological Studies; 54; 10; 10-1-2015; 1-9
1810-522X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/27953
identifier_str_mv Fernandez Alfaya, Jose Elias; Bigatti, Gregorio; Kajihara, Hiroshi; Strand, Malin; Sundberg, Per; et al.; DNA barcoding supports identification of Malacobdella species (Nemertea: Hoplonemertea); Springer; Zoological Studies; 54; 10; 10-1-2015; 1-9
1810-522X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/s40555-014-0086-3
info:eu-repo/semantics/altIdentifier/url/http://zoolstud.sinica.edu.tw/54.htm
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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