Production of the bioactive pigment elsinochrome A by a cultured mycobiont strain of the lichen Graphis elongata

Autores
Fazio, Alejandra Teresa; Adler, Monica Teresa; Parnmen, Sittiporn; Lücking, Robert; Maier, Marta Silvia
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We report the production of the perylenequinone pigment elsinochrome A in aposymbiotic culture of the mycobiont of the crustose epiphytic lichen Graphis elongata Zenker (Lecanoromycetes), collected in Argentina (Buenos Aires). The substance was not detected in the lichenized thallus (using HPLC techniques) and is otherwise only known from one unrelated lichen and a few genera of non-lichenized, plant-pathogenic fungi in the class Dothideomycetes. The phylogenetic affinities of the lichen mycobiont and the cultured fungus were confirmed using DNA sequence data of the mitochondrial small subunit rDNA (mtSSU), which place the lichen fungus into the Allographa clade within Graphidaceae. The mycobiont pigment was purified and characterized by spectroscopic methods. This is the first case where a rare pigment, otherwise known from non-lichenized, plant-pathogenic fungi, is produced in aposymbiotic culture of a lichen mycobiont, while, at the same time, being absent from the lichen thallus itself. Based on this finding, we discuss the previously postulated hypothesis that lichen mycobionts maintain secondary metabolic pathways of non-lichenized ancestors in their genome, while gene expression and production of metabolites is suppressed in the lichenized state due to toxicity to the photobiont.
Fil: Fazio, Alejandra Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: Adler, Monica Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: Parnmen, Sittiporn. National Institute of Health. Toxicology Center; Tailandia. Associate Researcher at the Field Museum; Estados Unidos
Fil: Lücking, Robert. Associate Researcher at the Field Museum; Estados Unidos. Botanic Garden and Botanical Museum; Alemania
Fil: Maier, Marta Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos en Química Orgánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Unidad de Microanálisis y Métodos Físicos en Química Orgánica; Argentina
Materia
AXENIC CULTURE
ELSINOCHROME A
GRAPHIS ELONGATA
LICHEN
PERYLENEQUINONES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/86688
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/86688
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Production of the bioactive pigment elsinochrome A by a cultured mycobiont strain of the lichen Graphis elongataFazio, Alejandra TeresaAdler, Monica TeresaParnmen, SittipornLücking, RobertMaier, Marta SilviaAXENIC CULTUREELSINOCHROME AGRAPHIS ELONGATALICHENPERYLENEQUINONEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1We report the production of the perylenequinone pigment elsinochrome A in aposymbiotic culture of the mycobiont of the crustose epiphytic lichen Graphis elongata Zenker (Lecanoromycetes), collected in Argentina (Buenos Aires). The substance was not detected in the lichenized thallus (using HPLC techniques) and is otherwise only known from one unrelated lichen and a few genera of non-lichenized, plant-pathogenic fungi in the class Dothideomycetes. The phylogenetic affinities of the lichen mycobiont and the cultured fungus were confirmed using DNA sequence data of the mitochondrial small subunit rDNA (mtSSU), which place the lichen fungus into the Allographa clade within Graphidaceae. The mycobiont pigment was purified and characterized by spectroscopic methods. This is the first case where a rare pigment, otherwise known from non-lichenized, plant-pathogenic fungi, is produced in aposymbiotic culture of a lichen mycobiont, while, at the same time, being absent from the lichen thallus itself. Based on this finding, we discuss the previously postulated hypothesis that lichen mycobionts maintain secondary metabolic pathways of non-lichenized ancestors in their genome, while gene expression and production of metabolites is suppressed in the lichenized state due to toxicity to the photobiont.Fil: Fazio, Alejandra Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaFil: Adler, Monica Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaFil: Parnmen, Sittiporn. National Institute of Health. Toxicology Center; Tailandia. Associate Researcher at the Field Museum; Estados UnidosFil: Lücking, Robert. Associate Researcher at the Field Museum; Estados Unidos. Botanic Garden and Botanical Museum; AlemaniaFil: Maier, Marta Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos en Química Orgánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Unidad de Microanálisis y Métodos Físicos en Química Orgánica; ArgentinaSpringer Heidelberg2018-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/86688Fazio, Alejandra Teresa; Adler, Monica Teresa; Parnmen, Sittiporn; Lücking, Robert; Maier, Marta Silvia; Production of the bioactive pigment elsinochrome A by a cultured mycobiont strain of the lichen Graphis elongata; Springer Heidelberg; Mycological Progress; 17; 4; 4-2018; 479-4871617-416X1861-8952CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s11557-017-1374-1info:eu-repo/semantics/altIdentifier/doi/10.1007/s11557-017-1374-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:36:06Zoai:ri.conicet.gov.ar:11336/86688instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:36:07.128CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Production of the bioactive pigment elsinochrome A by a cultured mycobiont strain of the lichen Graphis elongata
title Production of the bioactive pigment elsinochrome A by a cultured mycobiont strain of the lichen Graphis elongata
spellingShingle Production of the bioactive pigment elsinochrome A by a cultured mycobiont strain of the lichen Graphis elongata
Fazio, Alejandra Teresa
AXENIC CULTURE
ELSINOCHROME A
GRAPHIS ELONGATA
LICHEN
PERYLENEQUINONES
title_short Production of the bioactive pigment elsinochrome A by a cultured mycobiont strain of the lichen Graphis elongata
title_full Production of the bioactive pigment elsinochrome A by a cultured mycobiont strain of the lichen Graphis elongata
title_fullStr Production of the bioactive pigment elsinochrome A by a cultured mycobiont strain of the lichen Graphis elongata
title_full_unstemmed Production of the bioactive pigment elsinochrome A by a cultured mycobiont strain of the lichen Graphis elongata
title_sort Production of the bioactive pigment elsinochrome A by a cultured mycobiont strain of the lichen Graphis elongata
dc.creator.none.fl_str_mv Fazio, Alejandra Teresa
Adler, Monica Teresa
Parnmen, Sittiporn
Lücking, Robert
Maier, Marta Silvia
author Fazio, Alejandra Teresa
author_facet Fazio, Alejandra Teresa
Adler, Monica Teresa
Parnmen, Sittiporn
Lücking, Robert
Maier, Marta Silvia
author_role author
author2 Adler, Monica Teresa
Parnmen, Sittiporn
Lücking, Robert
Maier, Marta Silvia
author2_role author
author
author
author
dc.subject.none.fl_str_mv AXENIC CULTURE
ELSINOCHROME A
GRAPHIS ELONGATA
LICHEN
PERYLENEQUINONES
topic AXENIC CULTURE
ELSINOCHROME A
GRAPHIS ELONGATA
LICHEN
PERYLENEQUINONES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We report the production of the perylenequinone pigment elsinochrome A in aposymbiotic culture of the mycobiont of the crustose epiphytic lichen Graphis elongata Zenker (Lecanoromycetes), collected in Argentina (Buenos Aires). The substance was not detected in the lichenized thallus (using HPLC techniques) and is otherwise only known from one unrelated lichen and a few genera of non-lichenized, plant-pathogenic fungi in the class Dothideomycetes. The phylogenetic affinities of the lichen mycobiont and the cultured fungus were confirmed using DNA sequence data of the mitochondrial small subunit rDNA (mtSSU), which place the lichen fungus into the Allographa clade within Graphidaceae. The mycobiont pigment was purified and characterized by spectroscopic methods. This is the first case where a rare pigment, otherwise known from non-lichenized, plant-pathogenic fungi, is produced in aposymbiotic culture of a lichen mycobiont, while, at the same time, being absent from the lichen thallus itself. Based on this finding, we discuss the previously postulated hypothesis that lichen mycobionts maintain secondary metabolic pathways of non-lichenized ancestors in their genome, while gene expression and production of metabolites is suppressed in the lichenized state due to toxicity to the photobiont.
Fil: Fazio, Alejandra Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: Adler, Monica Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: Parnmen, Sittiporn. National Institute of Health. Toxicology Center; Tailandia. Associate Researcher at the Field Museum; Estados Unidos
Fil: Lücking, Robert. Associate Researcher at the Field Museum; Estados Unidos. Botanic Garden and Botanical Museum; Alemania
Fil: Maier, Marta Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos en Química Orgánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Unidad de Microanálisis y Métodos Físicos en Química Orgánica; Argentina
description We report the production of the perylenequinone pigment elsinochrome A in aposymbiotic culture of the mycobiont of the crustose epiphytic lichen Graphis elongata Zenker (Lecanoromycetes), collected in Argentina (Buenos Aires). The substance was not detected in the lichenized thallus (using HPLC techniques) and is otherwise only known from one unrelated lichen and a few genera of non-lichenized, plant-pathogenic fungi in the class Dothideomycetes. The phylogenetic affinities of the lichen mycobiont and the cultured fungus were confirmed using DNA sequence data of the mitochondrial small subunit rDNA (mtSSU), which place the lichen fungus into the Allographa clade within Graphidaceae. The mycobiont pigment was purified and characterized by spectroscopic methods. This is the first case where a rare pigment, otherwise known from non-lichenized, plant-pathogenic fungi, is produced in aposymbiotic culture of a lichen mycobiont, while, at the same time, being absent from the lichen thallus itself. Based on this finding, we discuss the previously postulated hypothesis that lichen mycobionts maintain secondary metabolic pathways of non-lichenized ancestors in their genome, while gene expression and production of metabolites is suppressed in the lichenized state due to toxicity to the photobiont.
publishDate 2018
dc.date.none.fl_str_mv 2018-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/86688
Fazio, Alejandra Teresa; Adler, Monica Teresa; Parnmen, Sittiporn; Lücking, Robert; Maier, Marta Silvia; Production of the bioactive pigment elsinochrome A by a cultured mycobiont strain of the lichen Graphis elongata; Springer Heidelberg; Mycological Progress; 17; 4; 4-2018; 479-487
1617-416X
1861-8952
CONICET Digital
CONICET
url http://hdl.handle.net/11336/86688
identifier_str_mv Fazio, Alejandra Teresa; Adler, Monica Teresa; Parnmen, Sittiporn; Lücking, Robert; Maier, Marta Silvia; Production of the bioactive pigment elsinochrome A by a cultured mycobiont strain of the lichen Graphis elongata; Springer Heidelberg; Mycological Progress; 17; 4; 4-2018; 479-487
1617-416X
1861-8952
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s11557-017-1374-1
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11557-017-1374-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Heidelberg
publisher.none.fl_str_mv Springer Heidelberg
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.216834

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