Usnic acid and Triacylglycerides Production by the Cultured Lichen Mycobiont of Ramalina celastri

Autores
Fazio, Alejandra Teresa; Adler, Monica Teresa; Maier, Marta Silvia
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A strain of the lichen mycobiont of Ramalina celastri, isolated from ascospores, was cultured axenically on two solid media containing high amounts of the carbon source: sucrose in MY10 and mannitol in BMRM. Usnic acid, the major cortical lichen metabolite, was produced by the colonies grown on MY10, with a very high yield (7.9%) in comparison with that in the lichen thallus. Mycelia grown on BMRM did not produce the lichen secondary metabolite and rendered triacylglycerides (8.5%) instead. Analysis by GC-MS of the fatty acid methyl esters revealed the presence of oleic, palmitic and stearic acids as the main triacylglyceride constituents. The present results highlight the impact of the culture conditions on the lichen mycobiont secondary metabolism and confirm that MY10 is a useful medium to obtain usnic acid from mycobionts in the laboratory.
Fil: Fazio, Alejandra Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: Adler, Monica Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: Maier, Marta Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos en Química Orgánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Unidad de Microanálisis y Métodos Físicos en Química Orgánica; Argentina
Materia
Usnic Acid
Triacylglycerides
Ramalina Celastri
Axenic Cultures
Lichen Metabolites
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/18717
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Usnic acid and Triacylglycerides Production by the Cultured Lichen Mycobiont of Ramalina celastriFazio, Alejandra TeresaAdler, Monica TeresaMaier, Marta SilviaUsnic AcidTriacylglyceridesRamalina CelastriAxenic CulturesLichen Metaboliteshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A strain of the lichen mycobiont of Ramalina celastri, isolated from ascospores, was cultured axenically on two solid media containing high amounts of the carbon source: sucrose in MY10 and mannitol in BMRM. Usnic acid, the major cortical lichen metabolite, was produced by the colonies grown on MY10, with a very high yield (7.9%) in comparison with that in the lichen thallus. Mycelia grown on BMRM did not produce the lichen secondary metabolite and rendered triacylglycerides (8.5%) instead. Analysis by GC-MS of the fatty acid methyl esters revealed the presence of oleic, palmitic and stearic acids as the main triacylglyceride constituents. The present results highlight the impact of the culture conditions on the lichen mycobiont secondary metabolism and confirm that MY10 is a useful medium to obtain usnic acid from mycobionts in the laboratory.Fil: Fazio, Alejandra Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaFil: Adler, Monica Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaFil: Maier, Marta Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos en Química Orgánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Unidad de Microanálisis y Métodos Físicos en Química Orgánica; ArgentinaNatural Products2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/18717Fazio, Alejandra Teresa; Adler, Monica Teresa; Maier, Marta Silvia; Usnic acid and Triacylglycerides Production by the Cultured Lichen Mycobiont of Ramalina celastri; Natural Products; Natural Product Communications; 9; 2; 2-2014; 213-2141934-578X1555-9475CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/abs/10.1177/1934578X1701200725info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:17:11Zoai:ri.conicet.gov.ar:11336/18717instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:17:12.083CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Usnic acid and Triacylglycerides Production by the Cultured Lichen Mycobiont of Ramalina celastri
title Usnic acid and Triacylglycerides Production by the Cultured Lichen Mycobiont of Ramalina celastri
spellingShingle Usnic acid and Triacylglycerides Production by the Cultured Lichen Mycobiont of Ramalina celastri
Fazio, Alejandra Teresa
Usnic Acid
Triacylglycerides
Ramalina Celastri
Axenic Cultures
Lichen Metabolites
title_short Usnic acid and Triacylglycerides Production by the Cultured Lichen Mycobiont of Ramalina celastri
title_full Usnic acid and Triacylglycerides Production by the Cultured Lichen Mycobiont of Ramalina celastri
title_fullStr Usnic acid and Triacylglycerides Production by the Cultured Lichen Mycobiont of Ramalina celastri
title_full_unstemmed Usnic acid and Triacylglycerides Production by the Cultured Lichen Mycobiont of Ramalina celastri
title_sort Usnic acid and Triacylglycerides Production by the Cultured Lichen Mycobiont of Ramalina celastri
dc.creator.none.fl_str_mv Fazio, Alejandra Teresa
Adler, Monica Teresa
Maier, Marta Silvia
author Fazio, Alejandra Teresa
author_facet Fazio, Alejandra Teresa
Adler, Monica Teresa
Maier, Marta Silvia
author_role author
author2 Adler, Monica Teresa
Maier, Marta Silvia
author2_role author
author
dc.subject.none.fl_str_mv Usnic Acid
Triacylglycerides
Ramalina Celastri
Axenic Cultures
Lichen Metabolites
topic Usnic Acid
Triacylglycerides
Ramalina Celastri
Axenic Cultures
Lichen Metabolites
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A strain of the lichen mycobiont of Ramalina celastri, isolated from ascospores, was cultured axenically on two solid media containing high amounts of the carbon source: sucrose in MY10 and mannitol in BMRM. Usnic acid, the major cortical lichen metabolite, was produced by the colonies grown on MY10, with a very high yield (7.9%) in comparison with that in the lichen thallus. Mycelia grown on BMRM did not produce the lichen secondary metabolite and rendered triacylglycerides (8.5%) instead. Analysis by GC-MS of the fatty acid methyl esters revealed the presence of oleic, palmitic and stearic acids as the main triacylglyceride constituents. The present results highlight the impact of the culture conditions on the lichen mycobiont secondary metabolism and confirm that MY10 is a useful medium to obtain usnic acid from mycobionts in the laboratory.
Fil: Fazio, Alejandra Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: Adler, Monica Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: Maier, Marta Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos en Química Orgánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Unidad de Microanálisis y Métodos Físicos en Química Orgánica; Argentina
description A strain of the lichen mycobiont of Ramalina celastri, isolated from ascospores, was cultured axenically on two solid media containing high amounts of the carbon source: sucrose in MY10 and mannitol in BMRM. Usnic acid, the major cortical lichen metabolite, was produced by the colonies grown on MY10, with a very high yield (7.9%) in comparison with that in the lichen thallus. Mycelia grown on BMRM did not produce the lichen secondary metabolite and rendered triacylglycerides (8.5%) instead. Analysis by GC-MS of the fatty acid methyl esters revealed the presence of oleic, palmitic and stearic acids as the main triacylglyceride constituents. The present results highlight the impact of the culture conditions on the lichen mycobiont secondary metabolism and confirm that MY10 is a useful medium to obtain usnic acid from mycobionts in the laboratory.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/18717
Fazio, Alejandra Teresa; Adler, Monica Teresa; Maier, Marta Silvia; Usnic acid and Triacylglycerides Production by the Cultured Lichen Mycobiont of Ramalina celastri; Natural Products; Natural Product Communications; 9; 2; 2-2014; 213-214
1934-578X
1555-9475
CONICET Digital
CONICET
url http://hdl.handle.net/11336/18717
identifier_str_mv Fazio, Alejandra Teresa; Adler, Monica Teresa; Maier, Marta Silvia; Usnic acid and Triacylglycerides Production by the Cultured Lichen Mycobiont of Ramalina celastri; Natural Products; Natural Product Communications; 9; 2; 2-2014; 213-214
1934-578X
1555-9475
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/abs/10.1177/1934578X1701200725
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Natural Products
publisher.none.fl_str_mv Natural Products
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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