Regulation of the Expression of the β-Lactam Antibiotic-Resistance Determinants in Methicillin-Resistant Staphylococcus aureus (MRSA)

Autores
Blázquez, Blas; Llarrull, Leticia Irene; Luque Ortega, Juan R.; Alfonso, Carlos; Boggess, Bill; Mobashery, Shahriar
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
β-Lactam antibiotics have faced obsolescence with the emergence of methicillin-resistant Staphylococcus aureus (MRSA). A complex set of events ensues upon exposure of MRSA to these antibiotics, which culminates in proteolysis of BlaI or MecI, two gene repressors, and results in the induction of resistance. We report studies on the mechanism of binding of these gene repressors to the operator regions by fluorescence anisotropy. Within the range of in vivo concentrations for BlaI and MecI, these proteins interact with their regulatory elements in a reversible manner, as both a monomer and a dimer.
Fil: Blázquez, Blas. University Of Notre Dame-Indiana; Estados Unidos
Fil: Llarrull, Leticia Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Luque Ortega, Juan R.. Consejo Superior de Investigaciones Cientificas; España
Fil: Alfonso, Carlos. Consejo Superior de Investigaciones Cientificas; España
Fil: Boggess, Bill. University Of Notre Dame-Indiana; Estados Unidos
Fil: Mobashery, Shahriar. University Of Notre Dame-Indiana; Estados Unidos
Materia
Methicillin-Resistant Staphylococcus Aureus
Blai
Meci
Regulation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/21382
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/21382
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Regulation of the Expression of the β-Lactam Antibiotic-Resistance Determinants in Methicillin-Resistant Staphylococcus aureus (MRSA)Blázquez, BlasLlarrull, Leticia IreneLuque Ortega, Juan R.Alfonso, CarlosBoggess, BillMobashery, ShahriarMethicillin-Resistant Staphylococcus AureusBlaiMeciRegulationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1β-Lactam antibiotics have faced obsolescence with the emergence of methicillin-resistant Staphylococcus aureus (MRSA). A complex set of events ensues upon exposure of MRSA to these antibiotics, which culminates in proteolysis of BlaI or MecI, two gene repressors, and results in the induction of resistance. We report studies on the mechanism of binding of these gene repressors to the operator regions by fluorescence anisotropy. Within the range of in vivo concentrations for BlaI and MecI, these proteins interact with their regulatory elements in a reversible manner, as both a monomer and a dimer.Fil: Blázquez, Blas. University Of Notre Dame-Indiana; Estados UnidosFil: Llarrull, Leticia Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Luque Ortega, Juan R.. Consejo Superior de Investigaciones Cientificas; EspañaFil: Alfonso, Carlos. Consejo Superior de Investigaciones Cientificas; EspañaFil: Boggess, Bill. University Of Notre Dame-Indiana; Estados UnidosFil: Mobashery, Shahriar. University Of Notre Dame-Indiana; Estados UnidosAmerican Chemical Society2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/21382Blázquez, Blas; Llarrull, Leticia Irene; Luque Ortega, Juan R.; Alfonso, Carlos; Boggess, Bill; et al.; Regulation of the Expression of the β-Lactam Antibiotic-Resistance Determinants in Methicillin-Resistant Staphylococcus aureus (MRSA); American Chemical Society; Biochemistry; 53; 10; 2-2014; 1548-15500006-2960CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1021/bi500074winfo:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/bi500074winfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:17Zoai:ri.conicet.gov.ar:11336/21382instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:17.55CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Regulation of the Expression of the β-Lactam Antibiotic-Resistance Determinants in Methicillin-Resistant Staphylococcus aureus (MRSA)
title Regulation of the Expression of the β-Lactam Antibiotic-Resistance Determinants in Methicillin-Resistant Staphylococcus aureus (MRSA)
spellingShingle Regulation of the Expression of the β-Lactam Antibiotic-Resistance Determinants in Methicillin-Resistant Staphylococcus aureus (MRSA)
Blázquez, Blas
Methicillin-Resistant Staphylococcus Aureus
Blai
Meci
Regulation
title_short Regulation of the Expression of the β-Lactam Antibiotic-Resistance Determinants in Methicillin-Resistant Staphylococcus aureus (MRSA)
title_full Regulation of the Expression of the β-Lactam Antibiotic-Resistance Determinants in Methicillin-Resistant Staphylococcus aureus (MRSA)
title_fullStr Regulation of the Expression of the β-Lactam Antibiotic-Resistance Determinants in Methicillin-Resistant Staphylococcus aureus (MRSA)
title_full_unstemmed Regulation of the Expression of the β-Lactam Antibiotic-Resistance Determinants in Methicillin-Resistant Staphylococcus aureus (MRSA)
title_sort Regulation of the Expression of the β-Lactam Antibiotic-Resistance Determinants in Methicillin-Resistant Staphylococcus aureus (MRSA)
dc.creator.none.fl_str_mv Blázquez, Blas
Llarrull, Leticia Irene
Luque Ortega, Juan R.
Alfonso, Carlos
Boggess, Bill
Mobashery, Shahriar
author Blázquez, Blas
author_facet Blázquez, Blas
Llarrull, Leticia Irene
Luque Ortega, Juan R.
Alfonso, Carlos
Boggess, Bill
Mobashery, Shahriar
author_role author
author2 Llarrull, Leticia Irene
Luque Ortega, Juan R.
Alfonso, Carlos
Boggess, Bill
Mobashery, Shahriar
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Methicillin-Resistant Staphylococcus Aureus
Blai
Meci
Regulation
topic Methicillin-Resistant Staphylococcus Aureus
Blai
Meci
Regulation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv β-Lactam antibiotics have faced obsolescence with the emergence of methicillin-resistant Staphylococcus aureus (MRSA). A complex set of events ensues upon exposure of MRSA to these antibiotics, which culminates in proteolysis of BlaI or MecI, two gene repressors, and results in the induction of resistance. We report studies on the mechanism of binding of these gene repressors to the operator regions by fluorescence anisotropy. Within the range of in vivo concentrations for BlaI and MecI, these proteins interact with their regulatory elements in a reversible manner, as both a monomer and a dimer.
Fil: Blázquez, Blas. University Of Notre Dame-Indiana; Estados Unidos
Fil: Llarrull, Leticia Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Luque Ortega, Juan R.. Consejo Superior de Investigaciones Cientificas; España
Fil: Alfonso, Carlos. Consejo Superior de Investigaciones Cientificas; España
Fil: Boggess, Bill. University Of Notre Dame-Indiana; Estados Unidos
Fil: Mobashery, Shahriar. University Of Notre Dame-Indiana; Estados Unidos
description β-Lactam antibiotics have faced obsolescence with the emergence of methicillin-resistant Staphylococcus aureus (MRSA). A complex set of events ensues upon exposure of MRSA to these antibiotics, which culminates in proteolysis of BlaI or MecI, two gene repressors, and results in the induction of resistance. We report studies on the mechanism of binding of these gene repressors to the operator regions by fluorescence anisotropy. Within the range of in vivo concentrations for BlaI and MecI, these proteins interact with their regulatory elements in a reversible manner, as both a monomer and a dimer.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/21382
Blázquez, Blas; Llarrull, Leticia Irene; Luque Ortega, Juan R.; Alfonso, Carlos; Boggess, Bill; et al.; Regulation of the Expression of the β-Lactam Antibiotic-Resistance Determinants in Methicillin-Resistant Staphylococcus aureus (MRSA); American Chemical Society; Biochemistry; 53; 10; 2-2014; 1548-1550
0006-2960
CONICET Digital
CONICET
url http://hdl.handle.net/11336/21382
identifier_str_mv Blázquez, Blas; Llarrull, Leticia Irene; Luque Ortega, Juan R.; Alfonso, Carlos; Boggess, Bill; et al.; Regulation of the Expression of the β-Lactam Antibiotic-Resistance Determinants in Methicillin-Resistant Staphylococcus aureus (MRSA); American Chemical Society; Biochemistry; 53; 10; 2-2014; 1548-1550
0006-2960
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1021/bi500074w
info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/bi500074w
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269572829282304
score 13.13397

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