Control of Brown Rot Produced by Monilinia fructicola in Peaches Using a Full-Spectrum Extract of Zuccagnia punctata Cav

Autores
Di Liberto, Melina Gabriela; Stegmayer, María Inés; Fernandez, Laura Noemí; Quiroga, Ariel Dario; Svetaz, Laura Andrea; Derita, Marcos Gabriel
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Brown rot of stone fruit, caused by Monilinia spp., is one of the most important diseases worldwide, causing significant production losses. Currently, the standard practices for controlling this infection consist of repetitive use of synthetic fungicides. The global tendency encourages the demand for high-quality food products harmless to health and the environment, leading to a reduction in the use of these types of substances. Zuccagnia punctata (Fabaceae) is a perennial shrub extensively used for the treatment of fungal and bacterial infections in Argentinean traditional medicine. In this study, we isolated and characterized (morphologically and molecularly) a pathogenic and virulent strain of Monilinia fructicola, which is the most hostile species of the genus. Consequently, we explored the in vitro antifungal activity of the ethanolic extract of Z. punctata against this phytopathogen. The chalcones 2′,4′-dihydroxy-3′-methoxychalcone and 2′,4′-dihydroxychalcone were isolated from the extract and evaluated against M. fructicola demonstrating that they were responsible for this activity. To promote full-spectrum extract rather than pure compounds, we performed ex-vivo assays using fresh peaches inoculated with the pathogen, and then treated by immersion in an extract solution of 250 µg/mL concentration. Treatment with Z. punctata extract did not show a statistically significant difference from commercial fungicides in the control of fruit rot. In addition, Huh7 cell cytotoxicity evaluation showed that Z. punctata extract was less cytotoxic than commercial fungicides at the assayed concentrations. Based on our research, this plant extract could potentially offer a safer alternative to commercial fungicides for treating peach brown rot.
Fil: Di Liberto, Melina Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Stegmayer, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral; Argentina
Fil: Fernandez, Laura Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral; Argentina
Fil: Quiroga, Ariel Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Fisiología Experimental. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental; Argentina
Fil: Svetaz, Laura Andrea. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Derita, Marcos Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral; Argentina
Materia
ANTIFUNGAL
BROWN ROT
CHALCONES
MONILINIA
PEACH
PHYTOPATHOGENIC FUNGI
ZUCCAGNIA PUNCTATA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/224708
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/224708
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Control of Brown Rot Produced by Monilinia fructicola in Peaches Using a Full-Spectrum Extract of Zuccagnia punctata CavDi Liberto, Melina GabrielaStegmayer, María InésFernandez, Laura NoemíQuiroga, Ariel DarioSvetaz, Laura AndreaDerita, Marcos GabrielANTIFUNGALBROWN ROTCHALCONESMONILINIAPEACHPHYTOPATHOGENIC FUNGIZUCCAGNIA PUNCTATAhttps://purl.org/becyt/ford/4.5https://purl.org/becyt/ford/4Brown rot of stone fruit, caused by Monilinia spp., is one of the most important diseases worldwide, causing significant production losses. Currently, the standard practices for controlling this infection consist of repetitive use of synthetic fungicides. The global tendency encourages the demand for high-quality food products harmless to health and the environment, leading to a reduction in the use of these types of substances. Zuccagnia punctata (Fabaceae) is a perennial shrub extensively used for the treatment of fungal and bacterial infections in Argentinean traditional medicine. In this study, we isolated and characterized (morphologically and molecularly) a pathogenic and virulent strain of Monilinia fructicola, which is the most hostile species of the genus. Consequently, we explored the in vitro antifungal activity of the ethanolic extract of Z. punctata against this phytopathogen. The chalcones 2′,4′-dihydroxy-3′-methoxychalcone and 2′,4′-dihydroxychalcone were isolated from the extract and evaluated against M. fructicola demonstrating that they were responsible for this activity. To promote full-spectrum extract rather than pure compounds, we performed ex-vivo assays using fresh peaches inoculated with the pathogen, and then treated by immersion in an extract solution of 250 µg/mL concentration. Treatment with Z. punctata extract did not show a statistically significant difference from commercial fungicides in the control of fruit rot. In addition, Huh7 cell cytotoxicity evaluation showed that Z. punctata extract was less cytotoxic than commercial fungicides at the assayed concentrations. Based on our research, this plant extract could potentially offer a safer alternative to commercial fungicides for treating peach brown rot.Fil: Di Liberto, Melina Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Stegmayer, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral; ArgentinaFil: Fernandez, Laura Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral; ArgentinaFil: Quiroga, Ariel Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Fisiología Experimental. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental; ArgentinaFil: Svetaz, Laura Andrea. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Derita, Marcos Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral; ArgentinaMDPI2023-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/224708Di Liberto, Melina Gabriela; Stegmayer, María Inés; Fernandez, Laura Noemí; Quiroga, Ariel Dario; Svetaz, Laura Andrea; et al.; Control of Brown Rot Produced by Monilinia fructicola in Peaches Using a Full-Spectrum Extract of Zuccagnia punctata Cav; MDPI; Horticulturae; 9; 10; 10-2023; 1141-11542311-7524CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2311-7524/9/10/1141info:eu-repo/semantics/altIdentifier/doi/10.3390/horticulturae9101141info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:11Zoai:ri.conicet.gov.ar:11336/224708instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:12.048CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Control of Brown Rot Produced by Monilinia fructicola in Peaches Using a Full-Spectrum Extract of Zuccagnia punctata Cav
title Control of Brown Rot Produced by Monilinia fructicola in Peaches Using a Full-Spectrum Extract of Zuccagnia punctata Cav
spellingShingle Control of Brown Rot Produced by Monilinia fructicola in Peaches Using a Full-Spectrum Extract of Zuccagnia punctata Cav
Di Liberto, Melina Gabriela
ANTIFUNGAL
BROWN ROT
CHALCONES
MONILINIA
PEACH
PHYTOPATHOGENIC FUNGI
ZUCCAGNIA PUNCTATA
title_short Control of Brown Rot Produced by Monilinia fructicola in Peaches Using a Full-Spectrum Extract of Zuccagnia punctata Cav
title_full Control of Brown Rot Produced by Monilinia fructicola in Peaches Using a Full-Spectrum Extract of Zuccagnia punctata Cav
title_fullStr Control of Brown Rot Produced by Monilinia fructicola in Peaches Using a Full-Spectrum Extract of Zuccagnia punctata Cav
title_full_unstemmed Control of Brown Rot Produced by Monilinia fructicola in Peaches Using a Full-Spectrum Extract of Zuccagnia punctata Cav
title_sort Control of Brown Rot Produced by Monilinia fructicola in Peaches Using a Full-Spectrum Extract of Zuccagnia punctata Cav
dc.creator.none.fl_str_mv Di Liberto, Melina Gabriela
Stegmayer, María Inés
Fernandez, Laura Noemí
Quiroga, Ariel Dario
Svetaz, Laura Andrea
Derita, Marcos Gabriel
author Di Liberto, Melina Gabriela
author_facet Di Liberto, Melina Gabriela
Stegmayer, María Inés
Fernandez, Laura Noemí
Quiroga, Ariel Dario
Svetaz, Laura Andrea
Derita, Marcos Gabriel
author_role author
author2 Stegmayer, María Inés
Fernandez, Laura Noemí
Quiroga, Ariel Dario
Svetaz, Laura Andrea
Derita, Marcos Gabriel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ANTIFUNGAL
BROWN ROT
CHALCONES
MONILINIA
PEACH
PHYTOPATHOGENIC FUNGI
ZUCCAGNIA PUNCTATA
topic ANTIFUNGAL
BROWN ROT
CHALCONES
MONILINIA
PEACH
PHYTOPATHOGENIC FUNGI
ZUCCAGNIA PUNCTATA
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.5
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Brown rot of stone fruit, caused by Monilinia spp., is one of the most important diseases worldwide, causing significant production losses. Currently, the standard practices for controlling this infection consist of repetitive use of synthetic fungicides. The global tendency encourages the demand for high-quality food products harmless to health and the environment, leading to a reduction in the use of these types of substances. Zuccagnia punctata (Fabaceae) is a perennial shrub extensively used for the treatment of fungal and bacterial infections in Argentinean traditional medicine. In this study, we isolated and characterized (morphologically and molecularly) a pathogenic and virulent strain of Monilinia fructicola, which is the most hostile species of the genus. Consequently, we explored the in vitro antifungal activity of the ethanolic extract of Z. punctata against this phytopathogen. The chalcones 2′,4′-dihydroxy-3′-methoxychalcone and 2′,4′-dihydroxychalcone were isolated from the extract and evaluated against M. fructicola demonstrating that they were responsible for this activity. To promote full-spectrum extract rather than pure compounds, we performed ex-vivo assays using fresh peaches inoculated with the pathogen, and then treated by immersion in an extract solution of 250 µg/mL concentration. Treatment with Z. punctata extract did not show a statistically significant difference from commercial fungicides in the control of fruit rot. In addition, Huh7 cell cytotoxicity evaluation showed that Z. punctata extract was less cytotoxic than commercial fungicides at the assayed concentrations. Based on our research, this plant extract could potentially offer a safer alternative to commercial fungicides for treating peach brown rot.
Fil: Di Liberto, Melina Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Stegmayer, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral; Argentina
Fil: Fernandez, Laura Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral; Argentina
Fil: Quiroga, Ariel Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Fisiología Experimental. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental; Argentina
Fil: Svetaz, Laura Andrea. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Derita, Marcos Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral; Argentina
description Brown rot of stone fruit, caused by Monilinia spp., is one of the most important diseases worldwide, causing significant production losses. Currently, the standard practices for controlling this infection consist of repetitive use of synthetic fungicides. The global tendency encourages the demand for high-quality food products harmless to health and the environment, leading to a reduction in the use of these types of substances. Zuccagnia punctata (Fabaceae) is a perennial shrub extensively used for the treatment of fungal and bacterial infections in Argentinean traditional medicine. In this study, we isolated and characterized (morphologically and molecularly) a pathogenic and virulent strain of Monilinia fructicola, which is the most hostile species of the genus. Consequently, we explored the in vitro antifungal activity of the ethanolic extract of Z. punctata against this phytopathogen. The chalcones 2′,4′-dihydroxy-3′-methoxychalcone and 2′,4′-dihydroxychalcone were isolated from the extract and evaluated against M. fructicola demonstrating that they were responsible for this activity. To promote full-spectrum extract rather than pure compounds, we performed ex-vivo assays using fresh peaches inoculated with the pathogen, and then treated by immersion in an extract solution of 250 µg/mL concentration. Treatment with Z. punctata extract did not show a statistically significant difference from commercial fungicides in the control of fruit rot. In addition, Huh7 cell cytotoxicity evaluation showed that Z. punctata extract was less cytotoxic than commercial fungicides at the assayed concentrations. Based on our research, this plant extract could potentially offer a safer alternative to commercial fungicides for treating peach brown rot.
publishDate 2023
dc.date.none.fl_str_mv 2023-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/224708
Di Liberto, Melina Gabriela; Stegmayer, María Inés; Fernandez, Laura Noemí; Quiroga, Ariel Dario; Svetaz, Laura Andrea; et al.; Control of Brown Rot Produced by Monilinia fructicola in Peaches Using a Full-Spectrum Extract of Zuccagnia punctata Cav; MDPI; Horticulturae; 9; 10; 10-2023; 1141-1154
2311-7524
CONICET Digital
CONICET
url http://hdl.handle.net/11336/224708
identifier_str_mv Di Liberto, Melina Gabriela; Stegmayer, María Inés; Fernandez, Laura Noemí; Quiroga, Ariel Dario; Svetaz, Laura Andrea; et al.; Control of Brown Rot Produced by Monilinia fructicola in Peaches Using a Full-Spectrum Extract of Zuccagnia punctata Cav; MDPI; Horticulturae; 9; 10; 10-2023; 1141-1154
2311-7524
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2311-7524/9/10/1141
info:eu-repo/semantics/altIdentifier/doi/10.3390/horticulturae9101141
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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