Antifungal activity of Zuccagnia punctata Cav.: Evidence for the mechanism of action

Autores
Svetaz, Laura Andrea; Agüero, María Belén; Alvarez, Sandra; Luna, Lorena Celina; Feresin, Gabriela Egly; Derita, Marcos Gabriel; Tapia, Alejandro; Zacchino, Susana Alicia Stella
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Petroleum ether and dichloromethane extracts of fruits, aerial parts and exudate of Zuccagnia punctata Cav. (Fabaceae) showed moderate antifungal activities against the yeasts C. albicans, S. cerevisiae and C. neoformans (MICs: 62.5-250 μg/mL) and very strong antifungal activities against the dermatophytes M. gypseum, T. rubrum and T. mentagrophytes (MICs: 8-16 μg/mL) thus supporting the ethnopharmacological use of this plant. Antifungal activity-directed fractionation of active extracts by using bioautography led to the isolation of 2′,4′-dihydroxy-3′-methoxychalcone (1) and 2′,4′-dihydroxychalcone (2) as the compounds responsible for the antifungal activity. Second-order studies included MIC80, MIC 50 and MFC of both chalcones in an extended panel of clinical isolates of the most sensitive fungi, and also comprised a series of targeted assays. They showed that the most active chalcone 2 is fungicidal rather than fungistatic, does not disrupt the fungal membranes up to 4 x MFC and does not act by inhibiting the fungal cell wall. So, 2′,4′-dihydroxychalcone would act by a different mechanism of action than the antifungal drugs in current clinical use, such as amphotericin B, azoles or echinocandins, and thus appears to be very promising as a novel antifungal agent. © Georg Thieme Verlag KG Stuttgart.
Fil: Svetaz, Laura Andrea. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Departamento de Producción Vegetal. Cátedra de Cultivos Intensivos; Argentina. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Agüero, María Belén. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Alvarez, Sandra. Universidad Nacional de Rosario; Argentina
Fil: Luna, Lorena Celina. Universidad Nacional San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Feresin, Gabriela Egly. Universidad Nacional San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Derita, Marcos Gabriel. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Departamento de Producción Vegetal. Cátedra de Cultivos Intensivos; Argentina. Universidad Nacional de Rosario; Argentina
Fil: Tapia, Alejandro. Universidad Nacional San Juan; Argentina
Fil: Zacchino, Susana Alicia Stella. Universidad Nacional de Rosario; Argentina. Farmacognosia; Argentina
Materia
ANTIFUNGAL
BIOAUTOGRAPHY
CHALCONES
DENSITOMETRY
FABACEAE
MECHANISM OF ACTION
ZUCCAGNIA PUNCTATA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/99780
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/99780
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antifungal activity of Zuccagnia punctata Cav.: Evidence for the mechanism of actionSvetaz, Laura AndreaAgüero, María BelénAlvarez, SandraLuna, Lorena CelinaFeresin, Gabriela EglyDerita, Marcos GabrielTapia, AlejandroZacchino, Susana Alicia StellaANTIFUNGALBIOAUTOGRAPHYCHALCONESDENSITOMETRYFABACEAEMECHANISM OF ACTIONZUCCAGNIA PUNCTATAhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Petroleum ether and dichloromethane extracts of fruits, aerial parts and exudate of Zuccagnia punctata Cav. (Fabaceae) showed moderate antifungal activities against the yeasts C. albicans, S. cerevisiae and C. neoformans (MICs: 62.5-250 μg/mL) and very strong antifungal activities against the dermatophytes M. gypseum, T. rubrum and T. mentagrophytes (MICs: 8-16 μg/mL) thus supporting the ethnopharmacological use of this plant. Antifungal activity-directed fractionation of active extracts by using bioautography led to the isolation of 2′,4′-dihydroxy-3′-methoxychalcone (1) and 2′,4′-dihydroxychalcone (2) as the compounds responsible for the antifungal activity. Second-order studies included MIC80, MIC 50 and MFC of both chalcones in an extended panel of clinical isolates of the most sensitive fungi, and also comprised a series of targeted assays. They showed that the most active chalcone 2 is fungicidal rather than fungistatic, does not disrupt the fungal membranes up to 4 x MFC and does not act by inhibiting the fungal cell wall. So, 2′,4′-dihydroxychalcone would act by a different mechanism of action than the antifungal drugs in current clinical use, such as amphotericin B, azoles or echinocandins, and thus appears to be very promising as a novel antifungal agent. © Georg Thieme Verlag KG Stuttgart.Fil: Svetaz, Laura Andrea. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Departamento de Producción Vegetal. Cátedra de Cultivos Intensivos; Argentina. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Agüero, María Belén. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaFil: Alvarez, Sandra. Universidad Nacional de Rosario; ArgentinaFil: Luna, Lorena Celina. Universidad Nacional San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Feresin, Gabriela Egly. Universidad Nacional San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Derita, Marcos Gabriel. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Departamento de Producción Vegetal. Cátedra de Cultivos Intensivos; Argentina. Universidad Nacional de Rosario; ArgentinaFil: Tapia, Alejandro. Universidad Nacional San Juan; ArgentinaFil: Zacchino, Susana Alicia Stella. Universidad Nacional de Rosario; Argentina. Farmacognosia; ArgentinaGeorg Thieme Verlag Kg2007-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/99780Svetaz, Laura Andrea; Agüero, María Belén; Alvarez, Sandra; Luna, Lorena Celina; Feresin, Gabriela Egly; et al.; Antifungal activity of Zuccagnia punctata Cav.: Evidence for the mechanism of action; Georg Thieme Verlag Kg; Planta Medica; 73; 10; 8-2007; 1074-10800032-0943CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.thieme-connect.de/products/ejournals/pdf/10.1055/s-2007-981561.pdfinfo:eu-repo/semantics/altIdentifier/doi/10.1055/s-2007-981561info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:00:27Zoai:ri.conicet.gov.ar:11336/99780instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:00:27.53CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antifungal activity of Zuccagnia punctata Cav.: Evidence for the mechanism of action
title Antifungal activity of Zuccagnia punctata Cav.: Evidence for the mechanism of action
spellingShingle Antifungal activity of Zuccagnia punctata Cav.: Evidence for the mechanism of action
Svetaz, Laura Andrea
ANTIFUNGAL
BIOAUTOGRAPHY
CHALCONES
DENSITOMETRY
FABACEAE
MECHANISM OF ACTION
ZUCCAGNIA PUNCTATA
title_short Antifungal activity of Zuccagnia punctata Cav.: Evidence for the mechanism of action
title_full Antifungal activity of Zuccagnia punctata Cav.: Evidence for the mechanism of action
title_fullStr Antifungal activity of Zuccagnia punctata Cav.: Evidence for the mechanism of action
title_full_unstemmed Antifungal activity of Zuccagnia punctata Cav.: Evidence for the mechanism of action
title_sort Antifungal activity of Zuccagnia punctata Cav.: Evidence for the mechanism of action
dc.creator.none.fl_str_mv Svetaz, Laura Andrea
Agüero, María Belén
Alvarez, Sandra
Luna, Lorena Celina
Feresin, Gabriela Egly
Derita, Marcos Gabriel
Tapia, Alejandro
Zacchino, Susana Alicia Stella
author Svetaz, Laura Andrea
author_facet Svetaz, Laura Andrea
Agüero, María Belén
Alvarez, Sandra
Luna, Lorena Celina
Feresin, Gabriela Egly
Derita, Marcos Gabriel
Tapia, Alejandro
Zacchino, Susana Alicia Stella
author_role author
author2 Agüero, María Belén
Alvarez, Sandra
Luna, Lorena Celina
Feresin, Gabriela Egly
Derita, Marcos Gabriel
Tapia, Alejandro
Zacchino, Susana Alicia Stella
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIFUNGAL
BIOAUTOGRAPHY
CHALCONES
DENSITOMETRY
FABACEAE
MECHANISM OF ACTION
ZUCCAGNIA PUNCTATA
topic ANTIFUNGAL
BIOAUTOGRAPHY
CHALCONES
DENSITOMETRY
FABACEAE
MECHANISM OF ACTION
ZUCCAGNIA PUNCTATA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Petroleum ether and dichloromethane extracts of fruits, aerial parts and exudate of Zuccagnia punctata Cav. (Fabaceae) showed moderate antifungal activities against the yeasts C. albicans, S. cerevisiae and C. neoformans (MICs: 62.5-250 μg/mL) and very strong antifungal activities against the dermatophytes M. gypseum, T. rubrum and T. mentagrophytes (MICs: 8-16 μg/mL) thus supporting the ethnopharmacological use of this plant. Antifungal activity-directed fractionation of active extracts by using bioautography led to the isolation of 2′,4′-dihydroxy-3′-methoxychalcone (1) and 2′,4′-dihydroxychalcone (2) as the compounds responsible for the antifungal activity. Second-order studies included MIC80, MIC 50 and MFC of both chalcones in an extended panel of clinical isolates of the most sensitive fungi, and also comprised a series of targeted assays. They showed that the most active chalcone 2 is fungicidal rather than fungistatic, does not disrupt the fungal membranes up to 4 x MFC and does not act by inhibiting the fungal cell wall. So, 2′,4′-dihydroxychalcone would act by a different mechanism of action than the antifungal drugs in current clinical use, such as amphotericin B, azoles or echinocandins, and thus appears to be very promising as a novel antifungal agent. © Georg Thieme Verlag KG Stuttgart.
Fil: Svetaz, Laura Andrea. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Departamento de Producción Vegetal. Cátedra de Cultivos Intensivos; Argentina. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Agüero, María Belén. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Alvarez, Sandra. Universidad Nacional de Rosario; Argentina
Fil: Luna, Lorena Celina. Universidad Nacional San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Feresin, Gabriela Egly. Universidad Nacional San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Derita, Marcos Gabriel. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Departamento de Producción Vegetal. Cátedra de Cultivos Intensivos; Argentina. Universidad Nacional de Rosario; Argentina
Fil: Tapia, Alejandro. Universidad Nacional San Juan; Argentina
Fil: Zacchino, Susana Alicia Stella. Universidad Nacional de Rosario; Argentina. Farmacognosia; Argentina
description Petroleum ether and dichloromethane extracts of fruits, aerial parts and exudate of Zuccagnia punctata Cav. (Fabaceae) showed moderate antifungal activities against the yeasts C. albicans, S. cerevisiae and C. neoformans (MICs: 62.5-250 μg/mL) and very strong antifungal activities against the dermatophytes M. gypseum, T. rubrum and T. mentagrophytes (MICs: 8-16 μg/mL) thus supporting the ethnopharmacological use of this plant. Antifungal activity-directed fractionation of active extracts by using bioautography led to the isolation of 2′,4′-dihydroxy-3′-methoxychalcone (1) and 2′,4′-dihydroxychalcone (2) as the compounds responsible for the antifungal activity. Second-order studies included MIC80, MIC 50 and MFC of both chalcones in an extended panel of clinical isolates of the most sensitive fungi, and also comprised a series of targeted assays. They showed that the most active chalcone 2 is fungicidal rather than fungistatic, does not disrupt the fungal membranes up to 4 x MFC and does not act by inhibiting the fungal cell wall. So, 2′,4′-dihydroxychalcone would act by a different mechanism of action than the antifungal drugs in current clinical use, such as amphotericin B, azoles or echinocandins, and thus appears to be very promising as a novel antifungal agent. © Georg Thieme Verlag KG Stuttgart.
publishDate 2007
dc.date.none.fl_str_mv 2007-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/99780
Svetaz, Laura Andrea; Agüero, María Belén; Alvarez, Sandra; Luna, Lorena Celina; Feresin, Gabriela Egly; et al.; Antifungal activity of Zuccagnia punctata Cav.: Evidence for the mechanism of action; Georg Thieme Verlag Kg; Planta Medica; 73; 10; 8-2007; 1074-1080
0032-0943
CONICET Digital
CONICET
url http://hdl.handle.net/11336/99780
identifier_str_mv Svetaz, Laura Andrea; Agüero, María Belén; Alvarez, Sandra; Luna, Lorena Celina; Feresin, Gabriela Egly; et al.; Antifungal activity of Zuccagnia punctata Cav.: Evidence for the mechanism of action; Georg Thieme Verlag Kg; Planta Medica; 73; 10; 8-2007; 1074-1080
0032-0943
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.thieme-connect.de/products/ejournals/pdf/10.1055/s-2007-981561.pdf
info:eu-repo/semantics/altIdentifier/doi/10.1055/s-2007-981561
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Georg Thieme Verlag Kg
publisher.none.fl_str_mv Georg Thieme Verlag Kg
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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