Estimation of the Band Broadening Parameters in Single Detection Size Exclusion Chromatography: A Comparative Study of Various Column Combinations

Autores
Schnöll Bitai, Irene; Vega, Jorge Ruben; Mader, Christoph
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Alternative approaches for the determination of band broadening in size-exclusion chromatography based on the use of exponentially modified Gaussian (EMG) functionswere used to experimentally investigate the performance of two different column sets. In both cases, the columns were combined in order to cover the complete fractionation range (from 103 to 5×106 g mol-1), which is of interest in many applications. When analyzing experimental chromatograms the question of proper data treatment (especially the necessary smoothing routines) became obvious and is discussed accordingly. First results indicate that the exponential decay time of the EMG decreases and the standard deviation of its Gaussian component slightly increases (or remains almost constant) with increasing retention volumes. As a consequence, the total variance and the asymmetry of the EMG both decrease with the retention volume. A favorable agreement with independent experimental results (obtained by other researchers on the basis of analyzing ultra narrow standards) was found. Additionally, the skew was also investigated as a function of the retention volume and the trend was found to be in concordance with the predictions of theoretical models. The comparison with theoretical models is also discussed.
Fil: Schnöll Bitai, Irene. University of Vienna; Austria
Fil: Vega, Jorge Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Mader, Christoph. Isovolta AG; Austria
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/20768
Acceder
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spelling Estimation of the Band Broadening Parameters in Single Detection Size Exclusion Chromatography: A Comparative Study of Various Column CombinationsSchnöll Bitai, IreneVega, Jorge RubenMader, Christophhttps://purl.org/becyt/ford/2.4https://purl.org/becyt/ford/2Alternative approaches for the determination of band broadening in size-exclusion chromatography based on the use of exponentially modified Gaussian (EMG) functionswere used to experimentally investigate the performance of two different column sets. In both cases, the columns were combined in order to cover the complete fractionation range (from 103 to 5×106 g mol-1), which is of interest in many applications. When analyzing experimental chromatograms the question of proper data treatment (especially the necessary smoothing routines) became obvious and is discussed accordingly. First results indicate that the exponential decay time of the EMG decreases and the standard deviation of its Gaussian component slightly increases (or remains almost constant) with increasing retention volumes. As a consequence, the total variance and the asymmetry of the EMG both decrease with the retention volume. A favorable agreement with independent experimental results (obtained by other researchers on the basis of analyzing ultra narrow standards) was found. Additionally, the skew was also investigated as a function of the retention volume and the trend was found to be in concordance with the predictions of theoretical models. The comparison with theoretical models is also discussed.Fil: Schnöll Bitai, Irene. University of Vienna; AustriaFil: Vega, Jorge Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Mader, Christoph. Isovolta AG; AustriaElsevier Science2007-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/20768Schnöll Bitai, Irene; Vega, Jorge Ruben; Mader, Christoph; Estimation of the Band Broadening Parameters in Single Detection Size Exclusion Chromatography: A Comparative Study of Various Column Combinations; Elsevier Science; Analytica Chimica Acta; 604; 1; 12-2007; 9-170003-2670CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.aca.2007.06.002info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0003267007010082?via%3Dihubinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:53:17Zoai:ri.conicet.gov.ar:11336/20768instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:53:17.436CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Estimation of the Band Broadening Parameters in Single Detection Size Exclusion Chromatography: A Comparative Study of Various Column Combinations
title Estimation of the Band Broadening Parameters in Single Detection Size Exclusion Chromatography: A Comparative Study of Various Column Combinations
spellingShingle Estimation of the Band Broadening Parameters in Single Detection Size Exclusion Chromatography: A Comparative Study of Various Column Combinations
Schnöll Bitai, Irene
title_short Estimation of the Band Broadening Parameters in Single Detection Size Exclusion Chromatography: A Comparative Study of Various Column Combinations
title_full Estimation of the Band Broadening Parameters in Single Detection Size Exclusion Chromatography: A Comparative Study of Various Column Combinations
title_fullStr Estimation of the Band Broadening Parameters in Single Detection Size Exclusion Chromatography: A Comparative Study of Various Column Combinations
title_full_unstemmed Estimation of the Band Broadening Parameters in Single Detection Size Exclusion Chromatography: A Comparative Study of Various Column Combinations
title_sort Estimation of the Band Broadening Parameters in Single Detection Size Exclusion Chromatography: A Comparative Study of Various Column Combinations
dc.creator.none.fl_str_mv Schnöll Bitai, Irene
Vega, Jorge Ruben
Mader, Christoph
author Schnöll Bitai, Irene
author_facet Schnöll Bitai, Irene
Vega, Jorge Ruben
Mader, Christoph
author_role author
author2 Vega, Jorge Ruben
Mader, Christoph
author2_role author
author
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.4
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Alternative approaches for the determination of band broadening in size-exclusion chromatography based on the use of exponentially modified Gaussian (EMG) functionswere used to experimentally investigate the performance of two different column sets. In both cases, the columns were combined in order to cover the complete fractionation range (from 103 to 5×106 g mol-1), which is of interest in many applications. When analyzing experimental chromatograms the question of proper data treatment (especially the necessary smoothing routines) became obvious and is discussed accordingly. First results indicate that the exponential decay time of the EMG decreases and the standard deviation of its Gaussian component slightly increases (or remains almost constant) with increasing retention volumes. As a consequence, the total variance and the asymmetry of the EMG both decrease with the retention volume. A favorable agreement with independent experimental results (obtained by other researchers on the basis of analyzing ultra narrow standards) was found. Additionally, the skew was also investigated as a function of the retention volume and the trend was found to be in concordance with the predictions of theoretical models. The comparison with theoretical models is also discussed.
Fil: Schnöll Bitai, Irene. University of Vienna; Austria
Fil: Vega, Jorge Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Mader, Christoph. Isovolta AG; Austria
description Alternative approaches for the determination of band broadening in size-exclusion chromatography based on the use of exponentially modified Gaussian (EMG) functionswere used to experimentally investigate the performance of two different column sets. In both cases, the columns were combined in order to cover the complete fractionation range (from 103 to 5×106 g mol-1), which is of interest in many applications. When analyzing experimental chromatograms the question of proper data treatment (especially the necessary smoothing routines) became obvious and is discussed accordingly. First results indicate that the exponential decay time of the EMG decreases and the standard deviation of its Gaussian component slightly increases (or remains almost constant) with increasing retention volumes. As a consequence, the total variance and the asymmetry of the EMG both decrease with the retention volume. A favorable agreement with independent experimental results (obtained by other researchers on the basis of analyzing ultra narrow standards) was found. Additionally, the skew was also investigated as a function of the retention volume and the trend was found to be in concordance with the predictions of theoretical models. The comparison with theoretical models is also discussed.
publishDate 2007
dc.date.none.fl_str_mv 2007-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/20768
Schnöll Bitai, Irene; Vega, Jorge Ruben; Mader, Christoph; Estimation of the Band Broadening Parameters in Single Detection Size Exclusion Chromatography: A Comparative Study of Various Column Combinations; Elsevier Science; Analytica Chimica Acta; 604; 1; 12-2007; 9-17
0003-2670
CONICET Digital
CONICET
url http://hdl.handle.net/11336/20768
identifier_str_mv Schnöll Bitai, Irene; Vega, Jorge Ruben; Mader, Christoph; Estimation of the Band Broadening Parameters in Single Detection Size Exclusion Chromatography: A Comparative Study of Various Column Combinations; Elsevier Science; Analytica Chimica Acta; 604; 1; 12-2007; 9-17
0003-2670
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aca.2007.06.002
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0003267007010082?via%3Dihub
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.221938

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