Determination of the Band Broadening Function in Size Exclusion Chromatography with Light-scattering Detection

Autores
Yossen, Mariana Matilde; Vega, Jorge Ruben; Chang, Taihyun; Meira, Gregorio Raul
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This work describes the determination of the band broadening function (BBF) in a size exclusion chromatograph fitted with 2 mixed-gel columns, a light scattering (LS) detector, and a differential refractometer (DR). The raw data were the chromatograms of 4 narrow polystyrene standards. First, the interdetector volume shift was indirectly estimated from its upper and lower limiting values. Then, for each of the standards, their ‘‘local’’ BBFs were estimated by application of an existing theoretical method. Each local BBF is an assumed elution-volume invariant in the narrow ranges of the analyzed standards and is represented by an exponentially-modified Gaussian of standard deviation rBB and exponential decay sBB. Finally, a ‘‘global’’ BBF (valid for the complete fractionation range) was interpolated from the local BBF parameters. For increasing elution volumes, the global BBF exhibits an increasing rBB and a decreasing sBB. In addition, the asymmetry factor [sBB=rBB] and the global variance [r2 BB þ s2 BB] both decrease with elution volume.
Fil: Yossen, Mariana Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico Para la Industria Química; Argentina
Fil: Vega, Jorge Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Chang, Taihyun. Pohang University of Science and Technology; Corea del Sur
Fil: Meira, Gregorio Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Materia
Band Broadening
Differential Refractometer And Light Scattering Detector
Interdetector Volume
Hplc And Sec
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/18790
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/18790
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Determination of the Band Broadening Function in Size Exclusion Chromatography with Light-scattering DetectionYossen, Mariana MatildeVega, Jorge RubenChang, TaihyunMeira, Gregorio RaulBand BroadeningDifferential Refractometer And Light Scattering DetectorInterdetector VolumeHplc And Sechttps://purl.org/becyt/ford/2.4https://purl.org/becyt/ford/2This work describes the determination of the band broadening function (BBF) in a size exclusion chromatograph fitted with 2 mixed-gel columns, a light scattering (LS) detector, and a differential refractometer (DR). The raw data were the chromatograms of 4 narrow polystyrene standards. First, the interdetector volume shift was indirectly estimated from its upper and lower limiting values. Then, for each of the standards, their ‘‘local’’ BBFs were estimated by application of an existing theoretical method. Each local BBF is an assumed elution-volume invariant in the narrow ranges of the analyzed standards and is represented by an exponentially-modified Gaussian of standard deviation rBB and exponential decay sBB. Finally, a ‘‘global’’ BBF (valid for the complete fractionation range) was interpolated from the local BBF parameters. For increasing elution volumes, the global BBF exhibits an increasing rBB and a decreasing sBB. In addition, the asymmetry factor [sBB=rBB] and the global variance [r2 BB þ s2 BB] both decrease with elution volume.Fil: Yossen, Mariana Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico Para la Industria Química; ArgentinaFil: Vega, Jorge Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Chang, Taihyun. Pohang University of Science and Technology; Corea del SurFil: Meira, Gregorio Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaTaylor2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/18790Yossen, Mariana Matilde; Vega, Jorge Ruben; Chang, Taihyun; Meira, Gregorio Raul; Determination of the Band Broadening Function in Size Exclusion Chromatography with Light-scattering Detection; Taylor; Journal Of Liquid Chromatography And Related Technologies; 35; 1; 1-2012; 79-941082-6076CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.1080/10826076.2011.597061info:eu-repo/semantics/altIdentifier/doi/10.1080/10826076.2011.597061info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:13:39Zoai:ri.conicet.gov.ar:11336/18790instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:13:39.51CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Determination of the Band Broadening Function in Size Exclusion Chromatography with Light-scattering Detection
title Determination of the Band Broadening Function in Size Exclusion Chromatography with Light-scattering Detection
spellingShingle Determination of the Band Broadening Function in Size Exclusion Chromatography with Light-scattering Detection
Yossen, Mariana Matilde
Band Broadening
Differential Refractometer And Light Scattering Detector
Interdetector Volume
Hplc And Sec
title_short Determination of the Band Broadening Function in Size Exclusion Chromatography with Light-scattering Detection
title_full Determination of the Band Broadening Function in Size Exclusion Chromatography with Light-scattering Detection
title_fullStr Determination of the Band Broadening Function in Size Exclusion Chromatography with Light-scattering Detection
title_full_unstemmed Determination of the Band Broadening Function in Size Exclusion Chromatography with Light-scattering Detection
title_sort Determination of the Band Broadening Function in Size Exclusion Chromatography with Light-scattering Detection
dc.creator.none.fl_str_mv Yossen, Mariana Matilde
Vega, Jorge Ruben
Chang, Taihyun
Meira, Gregorio Raul
author Yossen, Mariana Matilde
author_facet Yossen, Mariana Matilde
Vega, Jorge Ruben
Chang, Taihyun
Meira, Gregorio Raul
author_role author
author2 Vega, Jorge Ruben
Chang, Taihyun
Meira, Gregorio Raul
author2_role author
author
author
dc.subject.none.fl_str_mv Band Broadening
Differential Refractometer And Light Scattering Detector
Interdetector Volume
Hplc And Sec
topic Band Broadening
Differential Refractometer And Light Scattering Detector
Interdetector Volume
Hplc And Sec
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.4
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv This work describes the determination of the band broadening function (BBF) in a size exclusion chromatograph fitted with 2 mixed-gel columns, a light scattering (LS) detector, and a differential refractometer (DR). The raw data were the chromatograms of 4 narrow polystyrene standards. First, the interdetector volume shift was indirectly estimated from its upper and lower limiting values. Then, for each of the standards, their ‘‘local’’ BBFs were estimated by application of an existing theoretical method. Each local BBF is an assumed elution-volume invariant in the narrow ranges of the analyzed standards and is represented by an exponentially-modified Gaussian of standard deviation rBB and exponential decay sBB. Finally, a ‘‘global’’ BBF (valid for the complete fractionation range) was interpolated from the local BBF parameters. For increasing elution volumes, the global BBF exhibits an increasing rBB and a decreasing sBB. In addition, the asymmetry factor [sBB=rBB] and the global variance [r2 BB þ s2 BB] both decrease with elution volume.
Fil: Yossen, Mariana Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico Para la Industria Química; Argentina
Fil: Vega, Jorge Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Chang, Taihyun. Pohang University of Science and Technology; Corea del Sur
Fil: Meira, Gregorio Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
description This work describes the determination of the band broadening function (BBF) in a size exclusion chromatograph fitted with 2 mixed-gel columns, a light scattering (LS) detector, and a differential refractometer (DR). The raw data were the chromatograms of 4 narrow polystyrene standards. First, the interdetector volume shift was indirectly estimated from its upper and lower limiting values. Then, for each of the standards, their ‘‘local’’ BBFs were estimated by application of an existing theoretical method. Each local BBF is an assumed elution-volume invariant in the narrow ranges of the analyzed standards and is represented by an exponentially-modified Gaussian of standard deviation rBB and exponential decay sBB. Finally, a ‘‘global’’ BBF (valid for the complete fractionation range) was interpolated from the local BBF parameters. For increasing elution volumes, the global BBF exhibits an increasing rBB and a decreasing sBB. In addition, the asymmetry factor [sBB=rBB] and the global variance [r2 BB þ s2 BB] both decrease with elution volume.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/18790
Yossen, Mariana Matilde; Vega, Jorge Ruben; Chang, Taihyun; Meira, Gregorio Raul; Determination of the Band Broadening Function in Size Exclusion Chromatography with Light-scattering Detection; Taylor; Journal Of Liquid Chromatography And Related Technologies; 35; 1; 1-2012; 79-94
1082-6076
CONICET Digital
CONICET
url http://hdl.handle.net/11336/18790
identifier_str_mv Yossen, Mariana Matilde; Vega, Jorge Ruben; Chang, Taihyun; Meira, Gregorio Raul; Determination of the Band Broadening Function in Size Exclusion Chromatography with Light-scattering Detection; Taylor; Journal Of Liquid Chromatography And Related Technologies; 35; 1; 1-2012; 79-94
1082-6076
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.1080/10826076.2011.597061
info:eu-repo/semantics/altIdentifier/doi/10.1080/10826076.2011.597061
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor
publisher.none.fl_str_mv Taylor
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083283853508608
score 13.221938

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