Band Broadening Function in Size Exclusion Chromatography of Polymers: Review of Some Recent Developments

Autores
Meira, Gregorio Raul; Netopilík, M.; Schnöll Bitai, I.; Potschka, M.; Vega, Jorge Ruben
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This article reviews some recent developments on the determination of the Band Broadening Function (BBF) in Size Exclusion Chromatography (SEC) of polymers. It was carried out in the frame of the IUPAC Project: "Data Treatment in Size Exclusion Chromatography of Polymers". The correction for band broadening (BB) is important for quantitative determinations of the molar mass distribution (MMD) of narrow-distributed (or highly multimodal) polymers, and of derived variables such as kinetic parameters. In the narrow range of a molar mass standard, the BBF is uniform and of positive skewness. In a broad chromatographic range, the BBF is non-uniform and skewed; and it can be adequately represented by an exponentially-modified Gaussian function (EMG) of 2 parameters that vary slightly with elution volume: an increasing Gaussian variance and a decreasing exponential decay. Additionally, the total BBF variance remains almost constant if not close to the total exclusion limit. The following methods for determining BBF parameters are reviewed: a) a direct method based on assuming Poisson-distributed MMDs; b) a direct method based on measuring the mass- and molar mass chromatograms of narrow standards; c) a theoretical method based on a stochastic model that is equivalent to the Giddings-Eyring model; and d) a theoretical method based on a deterministic model obtained through an extension of the classical van Deemter expression. Ideally, the correction for BB requires a robust numerical inversion algorithm. However, alternative simplified solutions are also possible.
Fil: Meira, Gregorio Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Netopilík, M.. Institute of Macromolecular Chemistry; República Checa
Fil: Schnöll Bitai, I.. Porzellangasse; Austria
Fil: Potschka, M.. Technische Universitat Wien; Austria
Fil: Vega, Jorge Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
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oai:ri.conicet.gov.ar:11336/25947
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spelling Band Broadening Function in Size Exclusion Chromatography of Polymers: Review of Some Recent DevelopmentsMeira, Gregorio RaulNetopilík, M.Schnöll Bitai, I.Potschka, M.Vega, Jorge Rubenhttps://purl.org/becyt/ford/2.4https://purl.org/becyt/ford/2This article reviews some recent developments on the determination of the Band Broadening Function (BBF) in Size Exclusion Chromatography (SEC) of polymers. It was carried out in the frame of the IUPAC Project: "Data Treatment in Size Exclusion Chromatography of Polymers". The correction for band broadening (BB) is important for quantitative determinations of the molar mass distribution (MMD) of narrow-distributed (or highly multimodal) polymers, and of derived variables such as kinetic parameters. In the narrow range of a molar mass standard, the BBF is uniform and of positive skewness. In a broad chromatographic range, the BBF is non-uniform and skewed; and it can be adequately represented by an exponentially-modified Gaussian function (EMG) of 2 parameters that vary slightly with elution volume: an increasing Gaussian variance and a decreasing exponential decay. Additionally, the total BBF variance remains almost constant if not close to the total exclusion limit. The following methods for determining BBF parameters are reviewed: a) a direct method based on assuming Poisson-distributed MMDs; b) a direct method based on measuring the mass- and molar mass chromatograms of narrow standards; c) a theoretical method based on a stochastic model that is equivalent to the Giddings-Eyring model; and d) a theoretical method based on a deterministic model obtained through an extension of the classical van Deemter expression. Ideally, the correction for BB requires a robust numerical inversion algorithm. However, alternative simplified solutions are also possible.Fil: Meira, Gregorio Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Netopilík, M.. Institute of Macromolecular Chemistry; República ChecaFil: Schnöll Bitai, I.. Porzellangasse; AustriaFil: Potschka, M.. Technische Universitat Wien; AustriaFil: Vega, Jorge Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaWiley2007-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25947Meira, Gregorio Raul; Netopilík, M.; Schnöll Bitai, I.; Potschka, M.; Vega, Jorge Ruben; Band Broadening Function in Size Exclusion Chromatography of Polymers: Review of Some Recent Developments; Wiley; Macromolecular Symposia; 258; 1; 11-2007; 186-1971022-1360CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/masy.200751221info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/masy.200751221/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:45:59Zoai:ri.conicet.gov.ar:11336/25947instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:45:59.29CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Band Broadening Function in Size Exclusion Chromatography of Polymers: Review of Some Recent Developments
title Band Broadening Function in Size Exclusion Chromatography of Polymers: Review of Some Recent Developments
spellingShingle Band Broadening Function in Size Exclusion Chromatography of Polymers: Review of Some Recent Developments
Meira, Gregorio Raul
title_short Band Broadening Function in Size Exclusion Chromatography of Polymers: Review of Some Recent Developments
title_full Band Broadening Function in Size Exclusion Chromatography of Polymers: Review of Some Recent Developments
title_fullStr Band Broadening Function in Size Exclusion Chromatography of Polymers: Review of Some Recent Developments
title_full_unstemmed Band Broadening Function in Size Exclusion Chromatography of Polymers: Review of Some Recent Developments
title_sort Band Broadening Function in Size Exclusion Chromatography of Polymers: Review of Some Recent Developments
dc.creator.none.fl_str_mv Meira, Gregorio Raul
Netopilík, M.
Schnöll Bitai, I.
Potschka, M.
Vega, Jorge Ruben
author Meira, Gregorio Raul
author_facet Meira, Gregorio Raul
Netopilík, M.
Schnöll Bitai, I.
Potschka, M.
Vega, Jorge Ruben
author_role author
author2 Netopilík, M.
Schnöll Bitai, I.
Potschka, M.
Vega, Jorge Ruben
author2_role author
author
author
author
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.4
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv This article reviews some recent developments on the determination of the Band Broadening Function (BBF) in Size Exclusion Chromatography (SEC) of polymers. It was carried out in the frame of the IUPAC Project: "Data Treatment in Size Exclusion Chromatography of Polymers". The correction for band broadening (BB) is important for quantitative determinations of the molar mass distribution (MMD) of narrow-distributed (or highly multimodal) polymers, and of derived variables such as kinetic parameters. In the narrow range of a molar mass standard, the BBF is uniform and of positive skewness. In a broad chromatographic range, the BBF is non-uniform and skewed; and it can be adequately represented by an exponentially-modified Gaussian function (EMG) of 2 parameters that vary slightly with elution volume: an increasing Gaussian variance and a decreasing exponential decay. Additionally, the total BBF variance remains almost constant if not close to the total exclusion limit. The following methods for determining BBF parameters are reviewed: a) a direct method based on assuming Poisson-distributed MMDs; b) a direct method based on measuring the mass- and molar mass chromatograms of narrow standards; c) a theoretical method based on a stochastic model that is equivalent to the Giddings-Eyring model; and d) a theoretical method based on a deterministic model obtained through an extension of the classical van Deemter expression. Ideally, the correction for BB requires a robust numerical inversion algorithm. However, alternative simplified solutions are also possible.
Fil: Meira, Gregorio Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Netopilík, M.. Institute of Macromolecular Chemistry; República Checa
Fil: Schnöll Bitai, I.. Porzellangasse; Austria
Fil: Potschka, M.. Technische Universitat Wien; Austria
Fil: Vega, Jorge Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
description This article reviews some recent developments on the determination of the Band Broadening Function (BBF) in Size Exclusion Chromatography (SEC) of polymers. It was carried out in the frame of the IUPAC Project: "Data Treatment in Size Exclusion Chromatography of Polymers". The correction for band broadening (BB) is important for quantitative determinations of the molar mass distribution (MMD) of narrow-distributed (or highly multimodal) polymers, and of derived variables such as kinetic parameters. In the narrow range of a molar mass standard, the BBF is uniform and of positive skewness. In a broad chromatographic range, the BBF is non-uniform and skewed; and it can be adequately represented by an exponentially-modified Gaussian function (EMG) of 2 parameters that vary slightly with elution volume: an increasing Gaussian variance and a decreasing exponential decay. Additionally, the total BBF variance remains almost constant if not close to the total exclusion limit. The following methods for determining BBF parameters are reviewed: a) a direct method based on assuming Poisson-distributed MMDs; b) a direct method based on measuring the mass- and molar mass chromatograms of narrow standards; c) a theoretical method based on a stochastic model that is equivalent to the Giddings-Eyring model; and d) a theoretical method based on a deterministic model obtained through an extension of the classical van Deemter expression. Ideally, the correction for BB requires a robust numerical inversion algorithm. However, alternative simplified solutions are also possible.
publishDate 2007
dc.date.none.fl_str_mv 2007-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25947
Meira, Gregorio Raul; Netopilík, M.; Schnöll Bitai, I.; Potschka, M.; Vega, Jorge Ruben; Band Broadening Function in Size Exclusion Chromatography of Polymers: Review of Some Recent Developments; Wiley; Macromolecular Symposia; 258; 1; 11-2007; 186-197
1022-1360
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25947
identifier_str_mv Meira, Gregorio Raul; Netopilík, M.; Schnöll Bitai, I.; Potschka, M.; Vega, Jorge Ruben; Band Broadening Function in Size Exclusion Chromatography of Polymers: Review of Some Recent Developments; Wiley; Macromolecular Symposia; 258; 1; 11-2007; 186-197
1022-1360
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/masy.200751221
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/masy.200751221/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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