Genetic characterization of Escherichia coli isolated from cattle carcasses and feces in Mexico State

Autores
Reyes Rodríguez, Nydia E.; Soriano Vargas, Edgardo; Barba León, Jeannette; Navarro, Armando; Talavera Rojas, Martín; Sanso, Andrea Mariel; Bustamante, Ana Victoria
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Meat of bovine origin is one of the major vehicles in the transmission of verotoxigenic Escherichia coli (VTEC) to human consumers. This pathogen can produce serious human illness, including bloody diarrhea and hemolytic uremic syndrome. The aim of the current study was to characterize E. coli isolates (mainly VTEC strains) belonging to several serotypes in samples from cattle carcasses and feces of three municipal slaughter plants from Mexico State. The genetic diversity and molecular relatedness among the isolates was evaluated with multiple-locus variable-number tandem repeat analysis (MLVA). To our knowledge, and with the exception of E. coli O157:H7, this is the first time that serotypes analyzed here have been subtyped by MLVA in Mexico. MLVA typing grouped the 37 strains from this study into 30 distinct genotypes, 26 of which were unique. These findings indicate that cattle carcasses and feces from slaughter plants in Mexico are a source of VTEC that are genetically diverse in terms of serotypes and virulence profiles. The presence of these pathogens in carcasses indicates the high probability of the spread of VTEC strains during slaughter and processing.
Fil: Reyes Rodríguez, Nydia E.. Universidad Nacional Autónoma de México; México
Fil: Soriano Vargas, Edgardo. Universidad Nacional Autónoma de México; México
Fil: Barba León, Jeannette. Universidad de Guadalajara; México
Fil: Navarro, Armando. Universidad Nacional Autónoma de México; México
Fil: Talavera Rojas, Martín. Universidad Nacional Autónoma de México; México
Fil: Sanso, Andrea Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Bustamante, Ana Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Materia
Escherichia Coli
Genetic Characterization
Mlva
Cattle
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/51209
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/51209
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genetic characterization of Escherichia coli isolated from cattle carcasses and feces in Mexico StateReyes Rodríguez, Nydia E.Soriano Vargas, EdgardoBarba León, JeannetteNavarro, ArmandoTalavera Rojas, MartínSanso, Andrea MarielBustamante, Ana VictoriaEscherichia ColiGenetic CharacterizationMlvaCattlehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Meat of bovine origin is one of the major vehicles in the transmission of verotoxigenic Escherichia coli (VTEC) to human consumers. This pathogen can produce serious human illness, including bloody diarrhea and hemolytic uremic syndrome. The aim of the current study was to characterize E. coli isolates (mainly VTEC strains) belonging to several serotypes in samples from cattle carcasses and feces of three municipal slaughter plants from Mexico State. The genetic diversity and molecular relatedness among the isolates was evaluated with multiple-locus variable-number tandem repeat analysis (MLVA). To our knowledge, and with the exception of E. coli O157:H7, this is the first time that serotypes analyzed here have been subtyped by MLVA in Mexico. MLVA typing grouped the 37 strains from this study into 30 distinct genotypes, 26 of which were unique. These findings indicate that cattle carcasses and feces from slaughter plants in Mexico are a source of VTEC that are genetically diverse in terms of serotypes and virulence profiles. The presence of these pathogens in carcasses indicates the high probability of the spread of VTEC strains during slaughter and processing.Fil: Reyes Rodríguez, Nydia E.. Universidad Nacional Autónoma de México; MéxicoFil: Soriano Vargas, Edgardo. Universidad Nacional Autónoma de México; MéxicoFil: Barba León, Jeannette. Universidad de Guadalajara; MéxicoFil: Navarro, Armando. Universidad Nacional Autónoma de México; MéxicoFil: Talavera Rojas, Martín. Universidad Nacional Autónoma de México; MéxicoFil: Sanso, Andrea Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Bustamante, Ana Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaInt Assoc Food Protection2015-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/51209Reyes Rodríguez, Nydia E.; Soriano Vargas, Edgardo; Barba León, Jeannette; Navarro, Armando; Talavera Rojas, Martín; et al.; Genetic characterization of Escherichia coli isolated from cattle carcasses and feces in Mexico State; Int Assoc Food Protection; Journal of Food Protection; 78; 4; 4-2015; 796-8010362-028XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4315/0362-028X.JFP-14-425info:eu-repo/semantics/altIdentifier/url/http://jfoodprotection.org/doi/10.4315/0362-028X.JFP-14-425?code=fopr-siteinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:32:39Zoai:ri.conicet.gov.ar:11336/51209instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:32:39.338CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genetic characterization of Escherichia coli isolated from cattle carcasses and feces in Mexico State
title Genetic characterization of Escherichia coli isolated from cattle carcasses and feces in Mexico State
spellingShingle Genetic characterization of Escherichia coli isolated from cattle carcasses and feces in Mexico State
Reyes Rodríguez, Nydia E.
Escherichia Coli
Genetic Characterization
Mlva
Cattle
title_short Genetic characterization of Escherichia coli isolated from cattle carcasses and feces in Mexico State
title_full Genetic characterization of Escherichia coli isolated from cattle carcasses and feces in Mexico State
title_fullStr Genetic characterization of Escherichia coli isolated from cattle carcasses and feces in Mexico State
title_full_unstemmed Genetic characterization of Escherichia coli isolated from cattle carcasses and feces in Mexico State
title_sort Genetic characterization of Escherichia coli isolated from cattle carcasses and feces in Mexico State
dc.creator.none.fl_str_mv Reyes Rodríguez, Nydia E.
Soriano Vargas, Edgardo
Barba León, Jeannette
Navarro, Armando
Talavera Rojas, Martín
Sanso, Andrea Mariel
Bustamante, Ana Victoria
author Reyes Rodríguez, Nydia E.
author_facet Reyes Rodríguez, Nydia E.
Soriano Vargas, Edgardo
Barba León, Jeannette
Navarro, Armando
Talavera Rojas, Martín
Sanso, Andrea Mariel
Bustamante, Ana Victoria
author_role author
author2 Soriano Vargas, Edgardo
Barba León, Jeannette
Navarro, Armando
Talavera Rojas, Martín
Sanso, Andrea Mariel
Bustamante, Ana Victoria
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Escherichia Coli
Genetic Characterization
Mlva
Cattle
topic Escherichia Coli
Genetic Characterization
Mlva
Cattle
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Meat of bovine origin is one of the major vehicles in the transmission of verotoxigenic Escherichia coli (VTEC) to human consumers. This pathogen can produce serious human illness, including bloody diarrhea and hemolytic uremic syndrome. The aim of the current study was to characterize E. coli isolates (mainly VTEC strains) belonging to several serotypes in samples from cattle carcasses and feces of three municipal slaughter plants from Mexico State. The genetic diversity and molecular relatedness among the isolates was evaluated with multiple-locus variable-number tandem repeat analysis (MLVA). To our knowledge, and with the exception of E. coli O157:H7, this is the first time that serotypes analyzed here have been subtyped by MLVA in Mexico. MLVA typing grouped the 37 strains from this study into 30 distinct genotypes, 26 of which were unique. These findings indicate that cattle carcasses and feces from slaughter plants in Mexico are a source of VTEC that are genetically diverse in terms of serotypes and virulence profiles. The presence of these pathogens in carcasses indicates the high probability of the spread of VTEC strains during slaughter and processing.
Fil: Reyes Rodríguez, Nydia E.. Universidad Nacional Autónoma de México; México
Fil: Soriano Vargas, Edgardo. Universidad Nacional Autónoma de México; México
Fil: Barba León, Jeannette. Universidad de Guadalajara; México
Fil: Navarro, Armando. Universidad Nacional Autónoma de México; México
Fil: Talavera Rojas, Martín. Universidad Nacional Autónoma de México; México
Fil: Sanso, Andrea Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Bustamante, Ana Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
description Meat of bovine origin is one of the major vehicles in the transmission of verotoxigenic Escherichia coli (VTEC) to human consumers. This pathogen can produce serious human illness, including bloody diarrhea and hemolytic uremic syndrome. The aim of the current study was to characterize E. coli isolates (mainly VTEC strains) belonging to several serotypes in samples from cattle carcasses and feces of three municipal slaughter plants from Mexico State. The genetic diversity and molecular relatedness among the isolates was evaluated with multiple-locus variable-number tandem repeat analysis (MLVA). To our knowledge, and with the exception of E. coli O157:H7, this is the first time that serotypes analyzed here have been subtyped by MLVA in Mexico. MLVA typing grouped the 37 strains from this study into 30 distinct genotypes, 26 of which were unique. These findings indicate that cattle carcasses and feces from slaughter plants in Mexico are a source of VTEC that are genetically diverse in terms of serotypes and virulence profiles. The presence of these pathogens in carcasses indicates the high probability of the spread of VTEC strains during slaughter and processing.
publishDate 2015
dc.date.none.fl_str_mv 2015-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/51209
Reyes Rodríguez, Nydia E.; Soriano Vargas, Edgardo; Barba León, Jeannette; Navarro, Armando; Talavera Rojas, Martín; et al.; Genetic characterization of Escherichia coli isolated from cattle carcasses and feces in Mexico State; Int Assoc Food Protection; Journal of Food Protection; 78; 4; 4-2015; 796-801
0362-028X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/51209
identifier_str_mv Reyes Rodríguez, Nydia E.; Soriano Vargas, Edgardo; Barba León, Jeannette; Navarro, Armando; Talavera Rojas, Martín; et al.; Genetic characterization of Escherichia coli isolated from cattle carcasses and feces in Mexico State; Int Assoc Food Protection; Journal of Food Protection; 78; 4; 4-2015; 796-801
0362-028X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4315/0362-028X.JFP-14-425
info:eu-repo/semantics/altIdentifier/url/http://jfoodprotection.org/doi/10.4315/0362-028X.JFP-14-425?code=fopr-site
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Int Assoc Food Protection
publisher.none.fl_str_mv Int Assoc Food Protection
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614340330651648
score 13.070432

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